BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0003.p (220 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_904048.1| probable phosphatidylethanolamine N-methyltrans... 122 5e-27 ref|ZP_00008122.1| COG0500: SAM-dependent methyltransferases [Rh... 114 2e-24 sp|Q05197|PMTA_RHOSH Phosphatidylethanolamine N-methyltransferas... 107 3e-22 ref|NP_773274.1| pmtA [Bradyrhizobium japonicum] >gi|27354914|db... 99 6e-20 ref|ZP_00180050.1| hypothetical protein Mther202501 [Moorella th... 93 5e-18 >ref|NP_904048.1| probable phosphatidylethanolamine N-methyltransferase [Chromobacterium violaceum ATCC 12472] gb|AAQ62037.1| probable phosphatidylethanolamine N-methyltransferase [Chromobacterium violaceum ATCC 12472] Length = 205 Score = 122 bits (307), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 70/171 (40%), Positives = 106/171 (61%), Gaps = 5/171 (2%) Query: 17 NNSWNRIIYKLWAPLYDRVFNSGAFAGARKKALSGLDLKPGQQVLLVGAGTGADLPLISG 76 + +NR Y L+AP+YD+V + FA R++A+ L+ +P ++VLLVGAGTG DL + Sbjct: 6 STRFNRWRYDLYAPVYDKV--AQGFARHRRRAIGLLNPQPDERVLLVGAGTGLDLDFLV- 62 Query: 77 RGLSITAIDLSPAMLAEARSKI-KEGEKITFLEMDAQDLRFGDETFDVVIANLILSVVPD 135 R + AID++PAMLA R + K G ++ MD Q L F D +FD V +L+L+V+PD Sbjct: 63 RCRRVAAIDIAPAMLARLRERAGKLGMEVDARVMDGQRLDFPDGSFDAVALHLVLAVIPD 122 Query: 136 AERCMQEIVRVTSHGGKIVIFDKFASNGRVSPVM-KVMRPLISVLGTDIGR 185 C+ E+ RV GG++V+FDKF ++G+ P+ + L + TDI R Sbjct: 123 PRACLLEVERVLKPGGRVVVFDKFLADGKRPPLWRRAGNALARAVATDINR 173 >ref|ZP_00008122.1| COG0500: SAM-dependent methyltransferases [Rhodobacter sphaeroides] Length = 204 Score = 114 bits (285), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 73/192 (38%), Positives = 114/192 (59%), Gaps = 9/192 (4%) Query: 25 YKLWAPLYDRVFNSGAFAGARKKALSGLDLKPGQQVLLVGAGTGADLPLISGRGLSITAI 84 YK WAP+YD F+ G + + ++ + G +VL VG GTG LPL S R +++T I Sbjct: 10 YKRWAPIYD--FSFGKVSESPRRRTAAHVNARGGRVLEVGVGTGLSLPLYSHR-VAVTGI 66 Query: 85 DLSPAMLAEARSKIKEG--EKITFL-EMDAQDLRFGDETFDVVIANLILSVVPDAERCMQ 141 D S MLA AR K++E E + L +MDA++L F DETFD V+A ++SVVP+ ER + Sbjct: 67 DFSHEMLARAREKVEEMGLEPVKELRQMDARELDFPDETFDTVVAMFLVSVVPEPERVVS 126 Query: 142 EIVRVTSHGGKIVIFDKFASN-GRVSPVMKVMRPLISVLGTDIGRDFNLLFLPYNEQLSM 200 E+ RV GG++VI + FA + G ++ V K + + LG DF + + + +L + Sbjct: 127 EMARVCRKGGEVVIVNHFARDKGPLAAVEKALARFENTLGWH--SDFAIERVTADPRLEV 184 Query: 201 KEESPLLMRGMY 212 E++P+ G++ Sbjct: 185 VEQTPMSPGGLF 196 >sp|Q05197|PMTA_RHOSH Phosphatidylethanolamine N-methyltransferase pir||A47149 phosphatidylethanolamine N-methyltransferase (EC 2.1.1.17) [validated] - Rhodobacter sphaeroides gb|AAA26152.1| phosphatidylethanolamine N-methyltransferase Length = 203 Score = 107 bits (266), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 72/192 (37%), Positives = 113/192 (58%), Gaps = 10/192 (5%) Query: 25 YKLWAPLYDRVFNSGAFAGARKKALSGLDLKPGQQVLLVGAGTGADLPLISGRGLSITAI 84 YK WA +YD F+ G + + ++ + G +VL VG GTG LPL S R +++T I Sbjct: 10 YKRWA-IYD--FSFGKVSESPRRRTAAHVNARGGRVLEVGVGTGLSLPLYSHR-VAVTGI 65 Query: 85 DLSPAMLAEARSKIKEG--EKITFL-EMDAQDLRFGDETFDVVIANLILSVVPDAERCMQ 141 D S MLA AR K++E E + L +MDA++L F DETFD V+A ++SVVP+ ER + Sbjct: 66 DFSHEMLARAREKVEEMGLEPVKELRQMDARELDFPDETFDTVVAMFLVSVVPEPERVVS 125 Query: 142 EIVRVTSHGGKIVIFDKFASN-GRVSPVMKVMRPLISVLGTDIGRDFNLLFLPYNEQLSM 200 E+ RV GG++VI + FA + G ++ V K + + LG DF + + + +L + Sbjct: 126 EMARVCRKGGEVVIVNHFARDKGPLAAVEKALARFENTLGWH--SDFAIERVTADPRLEV 183 Query: 201 KEESPLLMRGMY 212 E++P+ G++ Sbjct: 184 VEQTPMSPGGLF 195 >ref|NP_773274.1| pmtA [Bradyrhizobium japonicum] dbj|BAC51899.1| pmtA [Bradyrhizobium japonicum USDA 110] Length = 229 Score = 99.4 bits (246), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 62/161 (38%), Positives = 97/161 (60%), Gaps = 9/161 (5%) Query: 25 YKLWAPLYDRVFNSGAFAGARKKALSGLDLKPGQQVLLVGAGTGADLPLISGRGLSITAI 84 Y WAP+YD VF G FA R+ A++ + K G +VL VG GTG LPL + L I Sbjct: 24 YDRWAPVYDLVFG-GVFAKGRQAAIAATN-KIGGRVLEVGVGTGISLPLYAP-NLRIFGT 80 Query: 85 DLSPAMLAEARSKIKEGEKITFLE----MDAQDLRFGDETFDVVIANLILSVVPDAERCM 140 D+S AML +AR ++ EG K+ +E MDA+ L F D +FDVV+A +++ VP+ E+ + Sbjct: 81 DISEAMLDKARQRVAEG-KLKNVEGLAVMDAEKLEFPDNSFDVVMAQYVVTAVPNPEKAL 139 Query: 141 QEIVRVTSHGGKIVIFDKFASN-GRVSPVMKVMRPLISVLG 180 E RV GG+++I + +++ G + + ++P++ LG Sbjct: 140 DEFARVLRPGGELIILTRVSADAGMRRFIEQKLQPVVRPLG 180 >ref|ZP_00180050.1| hypothetical protein Mther202501 [Moorella thermoacetica ATCC 39073] Length = 208 Score = 92.8 bits (229), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 53/130 (40%), Positives = 73/130 (56%), Gaps = 4/130 (3%) Query: 59 QVLLVGAGTGADLPLISGRGLSITAIDLSPAMLAEARSKIKEGE-KITFLEMDAQDLRFG 117 +VL VG GTGA+ P G +TAID SP MLA AR K+ + EMD Q L F Sbjct: 46 RVLEVGVGTGANFPFYPS-GCRVTAIDFSPGMLARARQKLHLARVPVDLREMDVQHLEFD 104 Query: 118 DETFDVVIANLILSVVPDAERCMQEIVRVTSHGGKIVIFDKFAS-NGRVSPVMKVMRPLI 176 TFD V+A + VPD R +QE+ RV GKIV+ + S + + P+M + PL+ Sbjct: 105 AATFDTVVATCVFCTVPDPVRGLQEVCRVCRPEGKIVLLEHVRSEHWLLGPLMDALNPLV 164 Query: 177 -SVLGTDIGR 185 ++G++I R Sbjct: 165 LYLIGSNINR 174 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.139 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 323,370,973 Number of extensions: 13300010 Number of successful extensions: 35985 Number of sequences better than 1.0e-05: 267 Number of HSP's gapped: 36529 Number of HSP's successfully gapped: 267 Length of query: 220 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 99 Effective length of database: 428,710,599 Effective search space: 42442349301 Effective search space used: 42442349301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)