BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0028.p (417 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832551.1| Macrolide glycosyltransferase [Bacillus cereus ... 240 5e-62 ref|NP_979129.1| glycosyltransferase, putative [Bacillus cereus ... 237 4e-61 ref|NP_831834.1| Macrolide glycosyltransferase [Bacillus cereus ... 234 3e-60 ref|NP_978481.1| glycosyltransferase, MGT family [Bacillus cereu... 230 5e-59 ref|NP_349322.1| Predicted glycosyl transferase from UDP-glucuro... 223 6e-57 >ref|NP_832551.1| Macrolide glycosyltransferase [Bacillus cereus ATCC 14579] gb|AAP09752.1| Macrolide glycosyltransferase [Bacillus cereus ATCC 14579] Length = 397 Score = 240 bits (612), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 140/415 (33%), Positives = 226/415 (54%), Gaps = 19/415 (4%) Query: 1 VSKIVFLSFPFYSHVMPTLTLVRELVSRGEEVIYYSNESFRETIEAMGVTFRSYGHARGM 60 +++++F++ H+ PTL +V EL+SRGEEV+Y+S E+FRE IE G T R+ + Sbjct: 1 MARVLFINAGSEGHINPTLQVVDELISRGEEVVYFSIEAFRERIEKTGATVRT------I 54 Query: 61 FDNSATTIEVEHPKVLLNYLVPGVIEKSKKIINAILPEVMADNPDYIIRDCDAFWGKQLA 120 D + + L + G++ + +I ++L ++ ++ DYII D G +A Sbjct: 55 DDQKFIKAFLSGGRNYLQERINGLLHTADIVIPSVLEQIEGEHFDYIIHDSMFGCGHLIA 114 Query: 121 SVLDVPVLCYVLTFAINKTMIDAAPLYFLENVFRIPPDKGQEYLDSLGKSRLSEELDVMS 180 +L +P + +FA ++ + + +N IP E D ++ + ++ Sbjct: 115 QILKLPAINSCTSFAQDEKSFEQMLGHLSKN---IPV----EIYD-----KIQNDFQNLT 162 Query: 181 KQIAMAHGIKDFDALDAFTGREELNIVYTTKEFQPFADTFDESYAFIGPNIRQDRTGVDS 240 K IA +G++ + + F L IVYT KEFQPF DTFDE Y F+GP+I D Sbjct: 163 KGIAEKYGVEIKSSYEVFCNPAPLTIVYTIKEFQPFGDTFDEIYKFVGPSISAQMKNRDV 222 Query: 241 RIEEMSGKPLIYISLGSIYTNRPGFYQKCFDAFRDTEWQIVMSIGSLVDIGDI-PVPDNF 299 + K IYISLG+++ FY+ C AF ++E IVMSIGS I D+ +P NF Sbjct: 223 DFTSIEEKSPIYISLGTVFNEAIDFYKLCMKAFENSEHTIVMSIGSKTKISDLGEIPKNF 282 Query: 300 YLSAFHPQMDVLERADVFITHGGYNSVCEAVHNNVPMIMFPQGADQFTISSHLDDMEAGI 359 + + PQ ++L +FITHGG NS E ++N VP+++ PQ ADQ ++ ++ + AGI Sbjct: 283 IVKNYVPQTELLTYTKLFITHGGMNSAHEGLYNGVPLVVIPQSADQPVVAKQVESLGAGI 342 Query: 360 YVRNSEISPRELRETAYKIYQDKKYKENGDKLKRSFQLSGGVRLAVDSIFSFLRQ 414 ++ ++ +L E+ + + +KE LK+SFQ SGG + AVD IF F+ Q Sbjct: 343 KLQMQGLTADQLSESVEMVLNNPSFKEVALNLKKSFQKSGGYKEAVDEIFIFVGQ 397 >ref|NP_979129.1| glycosyltransferase, putative [Bacillus cereus ATCC 10987] gb|AAS41737.1| glycosyltransferase, putative [Bacillus cereus ATCC 10987] Length = 397 Score = 237 bits (604), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 138/422 (32%), Positives = 230/422 (54%), Gaps = 33/422 (7%) Query: 1 VSKIVFLSFPFYSHVMPTLTLVRELVSRGEEVIYYSNESFRETIEAMGVTFRSYGHARGM 60 +++++F++ H+ PTL +V ELVSRGEEV+Y+S E+FRE IE G R+ R + Sbjct: 1 MARVLFINTGSEGHINPTLQVVEELVSRGEEVVYFSIEAFRERIEKTGAIVRTIDDQRFI 60 Query: 61 FDNSATTIEVEHPKVLL----NYL---VPGVIEKSKKIINAILPEVMADNPDYIIRDCDA 113 K L NYL + G++ + +I+++L ++ ++ DYII D Sbjct: 61 -------------KAFLSGGRNYLQERINGLLYTADVVISSVLEQIEGEHFDYIIHDSMF 107 Query: 114 FWGKQLASVLDVPVLCYVLTFAINKTMIDAAPLYFLENVFRIPPDKGQEYLDSLGKSRLS 173 G+ +A +L +P + +FA ++ + + +N+ P + Q+ + Sbjct: 108 GCGRLIAQILKLPAINSCTSFAQDERSFEQMLDHLSKNI----PVEVQD--------NIH 155 Query: 174 EELDVMSKQIAMAHGIKDFDALDAFTGREELNIVYTTKEFQPFADTFDESYAFIGPNIRQ 233 + + ++K I+ +G++ + F L IVYT KEFQPF DTF+E+Y F+GP+I Sbjct: 156 NDFERLTKGISEKYGVEINSPYEVFCNPAPLTIVYTVKEFQPFGDTFNETYKFVGPSISA 215 Query: 234 DRTGVDSRIEEMSGKPLIYISLGSIYTNRPGFYQKCFDAFRDTEWQIVMSIGSLVDIGDI 293 D + K IYISLG+++ FY+ C AF +++ IVMSIG+ +I D+ Sbjct: 216 QVKNEDFDFTSIEEKCPIYISLGTVFNEALDFYKLCMKAFENSDHTIVMSIGNKTNISDL 275 Query: 294 -PVPDNFYLSAFHPQMDVLERADVFITHGGYNSVCEAVHNNVPMIMFPQGADQFTISSHL 352 +P NF + + PQ +L +FITHGG NS E ++N +P+I+ PQ ADQ I+ + Sbjct: 276 GEIPKNFIVKNYVPQTKLLAYTKLFITHGGMNSTHEGLNNGIPLIVIPQSADQPVIAKQV 335 Query: 353 DDMEAGIYVRNSEISPRELRETAYKIYQDKKYKENGDKLKRSFQLSGGVRLAVDSIFSFL 412 +++ GI + E++ +LRE+ + + YKE LK SF+ SGG + A + IF+F+ Sbjct: 336 ENLGVGIKLSMKELTVEQLRESVELVLNNPSYKEAALHLKESFRKSGGYKEATNEIFTFI 395 Query: 413 RQ 414 Q Sbjct: 396 GQ 397 >ref|NP_831834.1| Macrolide glycosyltransferase [Bacillus cereus ATCC 14579] gb|AAP09035.1| Macrolide glycosyltransferase [Bacillus cereus ATCC 14579] Length = 402 Score = 234 bits (597), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 131/412 (31%), Positives = 230/412 (55%), Gaps = 20/412 (4%) Query: 1 VSKIVFLSFPFYSHVMPTLTLVRELVSRGEEVIYYSNESFRETIEAMGVTFRSYGHARGM 60 ++ ++ ++FP H+ PTL +V EL+ RGE V+ Y E +R+ +EA G FR + + Sbjct: 1 MANVLVINFPGEGHINPTLAVVSELIQRGETVVSYCIEDYRKKVEATGAEFRVFENFLSQ 60 Query: 61 FDNSATTIEVEHPKVLLNYLVPGVIEKSKKIINAILPEVMADNPDYIIRDCDAFWGKQLA 120 + E P +L+++ IE S++I+ I+ E + DY+I D G+ + Sbjct: 61 INIMERVNEGGSPLTMLSHM----IEASERIVTQIVEETKEEKYDYLIYDNHFPVGRIIV 116 Query: 121 SVLDVPVLCYVLTFAINKTMIDAAPLYFLENVFRIPPDKGQEYLDSLGKSRLSEELDVMS 180 ++L +P + TFA+N+ + N + + +D L +S L+ ++ + Sbjct: 117 NILQLPSVSSCTTFAVNQYI----------NFHDEQESRQVDEMDPLYQSCLAG-MERWN 165 Query: 181 KQIAMAHGIKDFDALDAFTGREELNIVYTTKEFQPFADTFDESYAFIGPNIRQDRTGVDS 240 KQ +G+K D ++ IVYT+KE+QP ++ FDESY F+GP+I + Sbjct: 166 KQ----YGMKCNSMYDIMNHPGDITIVYTSKEYQPRSEVFDESYKFVGPSIATRKEVGSF 221 Query: 241 RIEEMSGKPLIYISLGSIYTNRPGFYQKCFDAFRDTEWQIVMSIGSLVDIGDIP-VPDNF 299 E++ + +I+IS+G+++ +P Y+KCF+AF+D + +V+ +G ++I +P NF Sbjct: 222 PTEDLKNEKVIFISMGTVFNEQPALYEKCFEAFKDVDATVVLVVGKKINISQFENIPKNF 281 Query: 300 YLSAFHPQMDVLERADVFITHGGYNSVCEAVHNNVPMIMFPQGADQFTISSHLDDMEAGI 359 L + PQ++VL+ ADVF+THGG NS EA++ VP+++ P DQ ++ L ++ AGI Sbjct: 282 KLYNYVPQLEVLQHADVFVTHGGMNSSSEALYYGVPLVVIPVTGDQPFVAKRLTEVGAGI 341 Query: 360 YVRNSEISPRELRETAYKIYQDKKYKENGDKLKRSFQLSGGVRLAVDSIFSF 411 + +E++ LRET K+ D +KEN K+ S + +GG + AV+ IF Sbjct: 342 TLNRNELTSELLRETVKKVMDDVTFKENSLKVGESLRNAGGYQRAVEEIFKL 393 >ref|NP_978481.1| glycosyltransferase, MGT family [Bacillus cereus ATCC 10987] gb|AAS41089.1| glycosyltransferase, MGT family [Bacillus cereus ATCC 10987] Length = 400 Score = 230 bits (586), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 131/410 (31%), Positives = 226/410 (55%), Gaps = 20/410 (4%) Query: 1 VSKIVFLSFPFYSHVMPTLTLVRELVSRGEEVIYYSNESFRETIEAMGVTFRSYGHARGM 60 ++ ++ ++FP H+ PTL +V EL+ RGE V+ Y E +R+ IEA G FR + + Sbjct: 1 MANVLVINFPGEGHINPTLAIVSELIRRGETVVSYCIEDYRKKIEATGAQFRVFENFLSQ 60 Query: 61 FDNSATTIEVEHPKVLLNYLVPGVIEKSKKIINAILPEVMADNPDYIIRDCDAFWGKQLA 120 + E P +L+++ +E S++I+ I+ E + DY+I D G+ +A Sbjct: 61 INIMERVNEGGSPLTMLSHM----MEASERIVTQIVEETKGEKYDYLIYDNHFPVGRIIA 116 Query: 121 SVLDVPVLCYVLTFAINKTMIDAAPLYFLENVFRIPPDKGQEYLDSLGKSRLSEELDVMS 180 +VL +P + TFA N+ + + E+ R + Y L ++ + Sbjct: 117 NVLKLPSVSSCTTFAFNQYIT-----FNDEHESREVDETNPLYQSCLAG------MEKWN 165 Query: 181 KQIAMAHGIKDFDALDAFTGREELNIVYTTKEFQPFADTFDESYAFIGPNIRQDRTGVDS 240 KQ +G+K D ++ IVYT+KE+QP +D FDESY F+GP+I + Sbjct: 166 KQ----YGMKCNSMYDIMNHPGDITIVYTSKEYQPRSDVFDESYKFVGPSIATRKEVGSF 221 Query: 241 RIEEMSGKPLIYISLGSIYTNRPGFYQKCFDAFRDTEWQIVMSIGSLVDIGDIP-VPDNF 299 +E++ + LI+IS+G+++ +P Y+KCF+AF+D E +V+ +G ++I +P+NF Sbjct: 222 PMEDLKDEKLIFISMGTVFNEQPELYEKCFEAFKDVEATVVLVVGKKINISQFENIPNNF 281 Query: 300 YLSAFHPQMDVLERADVFITHGGYNSVCEAVHNNVPMIMFPQGADQFTISSHLDDMEAGI 359 L + PQ+++L+ ADVF+THGG NS EA++ VP+++ P DQ ++ ++++ AGI Sbjct: 282 KLYNYVPQLELLQYADVFVTHGGMNSSSEALYYGVPLVVIPVTGDQPLVAKRVNEVGAGI 341 Query: 360 YVRNSEISPRELRETAYKIYQDKKYKENGDKLKRSFQLSGGVRLAVDSIF 409 + E++ LRE+ K+ D +KE K+ S + +GG AVD I Sbjct: 342 RLNRKELTSEMLRESVKKVMDDVTFKEKSRKVGESLRNAGGYNRAVDEIL 391 >ref|NP_349322.1| Predicted glycosyl transferase from UDP-glucuronosyltransferase family [Clostridium acetobutylicum] pir||C97234 probable glycosyl transferase from UDP-glucuronosyltransferase family CAC2716 [imported] - Clostridium acetobutylicum gb|AAK80662.1| Predicted glycosyl transferase from UDP-glucuronosyltransferase family [Clostridium acetobutylicum] Length = 407 Score = 223 bits (568), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 140/419 (33%), Positives = 218/419 (52%), Gaps = 21/419 (5%) Query: 1 VSKIVFLSFPFYSHVMPTLTLVRELVSRGEEVIYYSNESFRETIEAMGVTFRSYGHARGM 60 +SK++F++FP Y HV PTL LV ELV RGEEV Y+ + F+E+IEA G F SYG Sbjct: 1 MSKVLFMNFPGYGHVNPTLGLVDELVKRGEEVYYFCTDEFKESIEAAGAKFISYGERMSN 60 Query: 61 FDNSATTIEVEHPKVLLNYLVPGVIEKSKKIINAILPEVMADNPDYIIRDCDAFW-GKQL 119 F N+ T ++ ++ G+ E I IL ++ DY++ C F G + Sbjct: 61 FKNANKTAGNRSYADIITKII-GMTECE---IEDILKKIKGMKFDYVVH-CSMFCAGNII 115 Query: 120 ASVLDVPVLCYVLTFAINKTMIDAAPLYFLENVFRIPPDKGQEYLDSLGKSRLSEELDVM 179 A +L VP + FA K ++ E P + Y S KSR+ + V Sbjct: 116 AQILKVPSISSFAVFATRKEVMKMQYSPITEKELESNPIVSRAY--SEAKSRIEKRYFVK 173 Query: 180 SKQIAMAHGIKDFDALDAFTGREELNIVYTTKEFQPFADTFDESYAFIGPNIRQDRTGVD 239 I+ + R +LNI YT KEF + +D+S+ FIGP I + + +D Sbjct: 174 MPAIS-----------ELMACRGDLNIAYTAKEFVSNIEYYDDSFKFIGPPIYKRKENLD 222 Query: 240 SRIEEMSGKPLIYISLGSIYTN-RPGFYQKCFDAFRDTEWQIVMSIGSLVDIGDIPVPDN 298 E++ K +IYISLG+++ N Y F+AF ++ +VMS + +D + +P N Sbjct: 223 FPFEKLKNKKVIYISLGTVFNNTNSNLYNIFFEAFGGSDVVVVMSAYN-IDTSNFNIPKN 281 Query: 299 FYLSAFHPQMDVLERADVFITHGGYNSVCEAVHNNVPMIMFPQGADQFTISSHLDDMEAG 358 F + + PQ ++L+ AD ITH G NS + ++N VP + P GADQ ++S ++ A Sbjct: 282 FIVRNYVPQTEILKYADAAITHAGMNSTSDLIYNKVPFVAIPIGADQLYMASRAKELGAA 341 Query: 359 IYVRNSEISPRELRETAYKIYQDKKYKENGDKLKRSFQLSGGVRLAVDSIFSFLRQKGI 417 I + +++ +ELRE+ K+ D Y EN +K++ F+ +GG R + IF F +KGI Sbjct: 342 IVLNKDKLTAKELRESVNKVMVDASYLENIEKIRDVFREAGGYRKGAEEIFKFKAEKGI 400 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 626,335,252 Number of extensions: 26926525 Number of successful extensions: 66962 Number of sequences better than 1.0e-05: 197 Number of HSP's gapped: 67103 Number of HSP's successfully gapped: 200 Length of query: 417 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 289 Effective length of database: 409,704,906 Effective search space: 118404717834 Effective search space used: 118404717834 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)