BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0070.p (332 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060101.1| COG1323: Predicted nucleotidyltransferase [Cl... 202 1e-50 ref|NP_623098.1| conserved hypothetical protein [Thermoanaerobac... 185 1e-45 ref|NP_980266.1| conserved hypothetical protein [Bacillus cereus... 182 7e-45 ref|NP_657961.1| hypothetical protein predicted by GeneMark [Bac... 182 9e-45 ref|NP_243451.1| BH2585~unknown conserved protein [Bacillus halo... 180 3e-44 >ref|ZP_00060101.1| COG1323: Predicted nucleotidyltransferase [Clostridium thermocellum ATCC 27405] Length = 415 Score = 202 bits (513), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 121/334 (36%), Positives = 181/334 (54%), Gaps = 16/334 (4%) Query: 1 LSGADRSIVVMSGPFTQRGEPAAVSKQARTEMALHMGADVVIELPVAYAVQPAEWFAFGA 60 +SGAD + VMSG F QRGEPA V+K ART+MAL GAD+VIELP++ A+ AE+FA GA Sbjct: 27 ISGADFVVCVMSGNFIQRGEPAIVNKWARTKMALSAGADLVIELPLSCAMASAEYFASGA 86 Query: 61 VALLEATGVVDSLCFGSEAGTLSALQPMAEFLADESSALQGEIRRRMALGXXXXXXXXXX 120 V +L G+VD +CFGSE G + L +A+ L +E + + ++ + G Sbjct: 87 VRILNDIGIVDYICFGSEHGDVKTLDYIAQILVEEPESYKSFLKEELDNGLSYPAARESA 146 Query: 121 XXXXXXXXXXXXXRRPGAAGLLRQPNNSLGLHYLIALRRLGSKIRPLTAPRTGAGFHDPL 180 ++ NN LG+ YL ALRR+ S I PLT R ++ Sbjct: 147 LKKYTAHSI-------NIPQIISSSNNILGIEYLKALRRIKSSIIPLTIKRINNDYNTEN 199 Query: 181 QSGSPIASATAIRRLLSEGGSP------AAYMPEYSMSVLEREHAAGRGPLNLESFRIPL 234 +GS I+SA++IR+ +S S A MP+ S+ +L E +AGRGP+ E F + Sbjct: 200 ITGS-ISSASSIRKYISTSNSTSFDDVLAMTMPKTSVDILFEEFSAGRGPVFKEDFYPVV 258 Query: 235 RHLLATRTAAELHGLQDMNEGLENRLLRVLPALPHFTVSGLLEALKSKRYTHTRLQRLLI 294 L+ T ++ ++EGLENR+ A T L+E++ ++RYT TR+QR+L+ Sbjct: 259 TSLIRKMTPEQIRNFAYVSEGLENRIKS--AADTAGTYEELVESICTRRYTKTRVQRILM 316 Query: 295 HALLNHSKQEMAPAVLAQGPGYIRVLGFRESGRQ 328 L+ + +++ P Y R+LGF G+Q Sbjct: 317 GILMGVTSKDLDMLSRFDSPQYARILGFNSKGKQ 350 >ref|NP_623098.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24702.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 401 Score = 185 bits (469), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 119/329 (36%), Positives = 175/329 (53%), Gaps = 18/329 (5%) Query: 1 LSGADRSIVVMSGPFTQRGEPAAVSKQARTEMALHMGADVVIELPVAYAVQPAEWFAFGA 60 L+ + VMSG F QRGEPA V K R EMAL G D+VIELPV YA AE FA+GA Sbjct: 27 LTKCQYVVAVMSGNFVQRGEPAIVDKWKRAEMALKAGVDLVIELPVVYATSTAENFAYGA 86 Query: 61 VALLEATGVVDSLCFGSEAGTLSALQPMAEFLADESSALQGEIRRRMALGXXXXXXXXXX 120 V LL++ V+D + FGSE G L L +A+ L +E + ++ + G Sbjct: 87 VKLLDSLKVIDCIAFGSEEGDLEKLSKIADILLEEPPDYKRALKENLGKGLTFAKARELA 146 Query: 121 XXXXXXXXXXXXXRRPGAAGLLRQPNNSLGLHYLIALRRLGSKIRPLTAPRTGAGFHDPL 180 + L+ NN LG+ YL AL+++ S I P T R GA + L Sbjct: 147 LVKVTGD--------ESISKTLQTSNNILGIEYLKALKKIKSSIVPFTIKRRGA-LYTSL 197 Query: 181 QSGSPIASATAIRRLLSEGGSPAA--YMPEYSMSVLEREHAAGRGPLNLESFRIPLRHLL 238 + ASAT+IR+ + E G A Y+P++++ +LER G+GP+ L+ F + +LL Sbjct: 198 KLEGEFASATSIRKAIEEKGIKAVKNYVPDFTLKILERAFKEGQGPVYLQDFSPIILYLL 257 Query: 239 ATRTAAELHGLQDMNEGLENRLLRVLPALPHFTVSGLLEALKSKRYTHTRLQRLLIHALL 298 R+ L + D+ EG++N++ + + + L++ KSKRYT +R++ +L+H LL Sbjct: 258 --RSGHPLENIFDVAEGIDNKIYKAASMTNN--IKDLIKLTKSKRYTESRIRHILLHLLL 313 Query: 299 NHSKQEMAPAVLAQGPGYIRVLGFRESGR 327 K+ GP YIRVLGF E G+ Sbjct: 314 KIDKKLFKE---FDGPNYIRVLGFSEKGK 339 >ref|NP_980266.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAS42874.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 393 Score = 182 bits (463), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 116/332 (34%), Positives = 170/332 (51%), Gaps = 18/332 (5%) Query: 1 LSGADRSIVVMSGPFTQRGEPAAVSKQARTEMALHMGADVVIELPVAYAVQPAEWFAFGA 60 L+ +D +I VMSGPF QRGEPA VSK RT+MAL G D+V+ELP A++ Q AE FA GA Sbjct: 7 LTHSDITIAVMSGPFLQRGEPALVSKWYRTKMALACGVDLVVELPYAFSTQKAETFANGA 66 Query: 61 VALLEATGVVDSLCFGSEAGTLSALQPMAEFLADESSALQGEIRRRMALGXXXXXXXXXX 120 +++L A V + +CFGSE G + +E +++ M G Sbjct: 67 ISILNALHVSE-ICFGSEDGQIENFYNTISVQKNEEETFNRLVKQFMNAGNSYAKATSEA 125 Query: 121 XXXXXXXXXXXXXRRPGAAGLLRQPNNSLGLHYLIALRRLGSKIRPLTAPRTGAGFHDPL 180 + QPNN LG Y+ A+ S ++ T R + +HD Sbjct: 126 FLHILSSEKNID---------MSQPNNILGFQYIKAILMQNSSMQAQTIKRFASHYHDET 176 Query: 181 QSGSPIASATAIRR-LLSEGGSPAA---YMPEYSMSVLEREHAAGRGPL-NLESFRIPLR 235 + IASAT+IR+ L SE S ++P+ + S+L + G L N E + + Sbjct: 177 FNDQHIASATSIRKQLFSENSSFTEIEPFIPKATASLLA-SYKQNYGTLHNWEQYFSFFK 235 Query: 236 HLLATRTAAELHGLQDMNEGLENRLLRVLPALPHFTVSGLLEALKSKRYTHTRLQRLLIH 295 + L T + +L + ++ EGLE+R+L + F +EALK+KRYT TRLQR H Sbjct: 236 YKLMTMSPEDLRHIYEIEEGLEHRILSKIQTSSSF--HSFMEALKTKRYTWTRLQRACTH 293 Query: 296 ALLNHSKQEMAPAVLAQGPGYIRVLGFRESGR 327 L N +K+E+ A + Q YIR+LG + G+ Sbjct: 294 ILTNTTKEEIHCANIEQHAPYIRLLGMSQKGQ 325 >ref|NP_657961.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846374.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP27860.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 393 Score = 182 bits (462), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 115/332 (34%), Positives = 170/332 (51%), Gaps = 18/332 (5%) Query: 1 LSGADRSIVVMSGPFTQRGEPAAVSKQARTEMALHMGADVVIELPVAYAVQPAEWFAFGA 60 L+ +D +I VMSGPF QRGEPA VSK RT+MAL G D+V+ELP A++ Q AE FA GA Sbjct: 7 LTHSDITIAVMSGPFLQRGEPALVSKWYRTKMALACGVDLVVELPYAFSTQKAETFANGA 66 Query: 61 VALLEATGVVDSLCFGSEAGTLSALQPMAEFLADESSALQGEIRRRMALGXXXXXXXXXX 120 +++L A V + +CFGSE G + +E +++ M G Sbjct: 67 ISILNALHVSE-ICFGSEDGQIENFYNTISVQKNEEETFNRLVKQFMNAGNSYAKATSEA 125 Query: 121 XXXXXXXXXXXXXRRPGAAGLLRQPNNSLGLHYLIALRRLGSKIRPLTAPRTGAGFHDPL 180 + QPNN LG Y+ A+ S ++ T R + +HD Sbjct: 126 FLHILSSEKNID---------MSQPNNILGFQYIKAILMQNSSMQAQTIKRFASHYHDET 176 Query: 181 QSGSPIASATAIRR-LLSEGGSPA---AYMPEYSMSVLEREHAAGRGPL-NLESFRIPLR 235 + IASAT+IR+ L SE S +++P+ + S+L + G L N E + + Sbjct: 177 FNDQHIASATSIRKQLFSENSSFTEIESFIPKATASLLA-SYKQNYGTLHNWEQYFSFFK 235 Query: 236 HLLATRTAAELHGLQDMNEGLENRLLRVLPALPHFTVSGLLEALKSKRYTHTRLQRLLIH 295 + L T + L + ++ EGLE+R+L + F +E+LK+KRYT TRLQR H Sbjct: 236 YKLMTMSPENLRHIYEIEEGLEHRILSKIQTSSSF--HSFMESLKTKRYTWTRLQRACTH 293 Query: 296 ALLNHSKQEMAPAVLAQGPGYIRVLGFRESGR 327 L N +K+E+ A + Q YIR+LG + G+ Sbjct: 294 ILTNTTKEEIYCANIEQHAPYIRLLGMSQKGQ 325 >ref|NP_243451.1| BH2585~unknown conserved protein [Bacillus halodurans] pir||A83973 hypothetical protein BH2585 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06304.1| BH2585~unknown conserved protein [Bacillus halodurans] Length = 416 Score = 180 bits (457), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 123/334 (36%), Positives = 173/334 (51%), Gaps = 23/334 (6%) Query: 2 SGADRSIVVMSGPFTQRGEPAAVSKQARTEMALHMGADVVIELPVAYAVQPAEWFAFGAV 61 + AD I VMSG F QRGEPA + K RT +AL GAD+V+ELP A++ Q AEWFA GAV Sbjct: 28 AKADVVIAVMSGYFLQRGEPAILPKWERTSLALQGGADLVVELPYAFSTQKAEWFATGAV 87 Query: 62 ALLEATGVVDSLCFGSEAGTLSALQPMAEFLADESSALQGEIRRRMALGXXXXXXXXXXX 121 ++L A D+LCFGSE GT+ + F+A A I+ + G Sbjct: 88 SILAALE-ADALCFGSEEGTIEPFHRLYHFMAKHRLAWDRMIKEELDKG----------- 135 Query: 122 XXXXXXXXXXXXRRPGAAGLLR--QPNNSLGLHYLIALRRLGSKIRPLTAPRTGAGFHDP 179 R G+A L +PNN LG HY+ A+ L + I+ +T PR AG+HD Sbjct: 136 MSYPTATSLAFKRLEGSAEHLDLSRPNNILGFHYVKAIYDLHTSIKAMTIPRIKAGYHDD 195 Query: 180 LQSGSPIASATAIRRLL--SEGGSPAA-YMPEYSMSVLEREHAAGRGPLNLESFRIPLRH 236 + S IASAT+IR+ L EG +P Y+ +L+ + E L++ Sbjct: 196 SLNESSIASATSIRKSLKTKEGWQMVDRVVPSYTTEMLKSFEKETTFLPSWERLFPLLKY 255 Query: 237 LLATRTAAELHGLQDMNEGLENRLLRVLPALPHFTVSGLLEALKSKRYTHTRLQRLLIHA 296 L T T +LH + + EGLE R L+ + + F + +K+KRYT TR+QR H Sbjct: 256 RLLTATPEQLHAIYEGEEGLEYRALKTIVSATSF--HDWMTKMKTKRYTWTRIQRYATHL 313 Query: 297 LLNHSKQEMAPAVLAQGPG---YIRVLGFRESGR 327 N +K+E+ +VL +G YIR+LG G+ Sbjct: 314 FTNTTKEEIH-SVLPRGTESLPYIRLLGMTSRGQ 346 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 423,478,826 Number of extensions: 15986202 Number of successful extensions: 34489 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 34422 Number of HSP's successfully gapped: 51 Length of query: 332 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 206 Effective length of database: 415,135,104 Effective search space: 85517831424 Effective search space used: 85517831424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)