BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0556.0003.p (228 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_657222.1| hypothetical protein predicted by GeneMark [Bac... 192 5e-48 ref|NP_845649.1| membrane protein, putative [Bacillus anthracis ... 192 6e-48 ref|NP_979622.1| membrane protein, putative [Bacillus cereus ATC... 191 2e-47 ref|NP_781418.1| conserved membrane protein [Clostridium tetani ... 119 6e-26 ref|ZP_00181173.1| hypothetical protein Mther075101 [Moorella th... 77 4e-13 >ref|NP_657222.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] Length = 233 Score = 192 bits (488), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 90/156 (57%), Positives = 115/156 (73%) Query: 73 RIPFAALPSVKPVSAIVILSAYVFGAEAGFIIGAVAALVSNIYFGQGPWTPWQMFAWGMV 132 R+PF+ LPSV+P S ++I+SA VFG+E GF+IGA AA+VSNI+ GQGPWTPWQMF+WGM+ Sbjct: 74 RVPFSILPSVQPTSFVIIVSAIVFGSETGFMIGATAAIVSNIFLGQGPWTPWQMFSWGMI 133 Query: 133 GLTAGWLRNTWWMKKKGGMLAFGFVWGFLFGWIMNIWYLISLPDAFSWGLVAAAYASSFY 192 G AG LRNT+ MKK G L +G GFLFGWIMN W L+ +W V + Y +SFY Sbjct: 134 GFIAGLLRNTFLMKKLWGRLLYGLFVGFLFGWIMNFWGLLGFIREATWETVISYYIASFY 193 Query: 193 LDLAHALSNVFFLAVLAGGWTKVLKRFRKKYGLLQE 228 DL+HA+SNV FL + + W +L RF+KKYG+L E Sbjct: 194 FDLSHAISNVVFLLLFSTSWITILTRFKKKYGILDE 229 >ref|NP_845649.1| membrane protein, putative [Bacillus anthracis str. Ames] gb|AAP27135.1| membrane protein, putative [Bacillus anthracis str. Ames] Length = 233 Score = 192 bits (488), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 90/156 (57%), Positives = 115/156 (73%) Query: 73 RIPFAALPSVKPVSAIVILSAYVFGAEAGFIIGAVAALVSNIYFGQGPWTPWQMFAWGMV 132 R+PF+ LPSV+P S ++I+SA VFG+E GF+IGA AA+VSNI+ GQGPWTPWQMF+WGM+ Sbjct: 74 RVPFSILPSVQPTSFVIIVSAIVFGSETGFMIGATAAIVSNIFLGQGPWTPWQMFSWGMI 133 Query: 133 GLTAGWLRNTWWMKKKGGMLAFGFVWGFLFGWIMNIWYLISLPDAFSWGLVAAAYASSFY 192 G AG LRNT+ MKK G L +G GFLFGWIMN W L+ +W V + Y +SFY Sbjct: 134 GFIAGLLRNTFLMKKLWGRLLYGLFVGFLFGWIMNFWGLLGFIREATWETVISYYIASFY 193 Query: 193 LDLAHALSNVFFLAVLAGGWTKVLKRFRKKYGLLQE 228 DL+HA+SNV FL + + W +L RF+KKYG+L E Sbjct: 194 FDLSHAISNVVFLLLFSTSWITILTRFKKKYGILDE 229 >ref|NP_979622.1| membrane protein, putative [Bacillus cereus ATCC 10987] gb|AAS42230.1| membrane protein, putative [Bacillus cereus ATCC 10987] Length = 233 Score = 191 bits (484), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 89/156 (57%), Positives = 115/156 (73%) Query: 73 RIPFAALPSVKPVSAIVILSAYVFGAEAGFIIGAVAALVSNIYFGQGPWTPWQMFAWGMV 132 R+PF+ LPSV+P S ++I+SA VFG+E GF+IGA AA+VSNI+ GQGPWTPWQMF+WGM+ Sbjct: 74 RVPFSVLPSVQPTSFVIIVSAIVFGSETGFMIGATAAIVSNIFLGQGPWTPWQMFSWGMI 133 Query: 133 GLTAGWLRNTWWMKKKGGMLAFGFVWGFLFGWIMNIWYLISLPDAFSWGLVAAAYASSFY 192 G AG LRNT+ MKK G L +G GFLFGWIMN W L+ +W V + Y +SFY Sbjct: 134 GFIAGLLRNTFLMKKLWGRLLYGLFVGFLFGWIMNFWGLLGFIREATWETVISYYIASFY 193 Query: 193 LDLAHALSNVFFLAVLAGGWTKVLKRFRKKYGLLQE 228 DL+HA+SNV FL + + W +L RF+KKYG+L + Sbjct: 194 FDLSHAISNVVFLLLFSTSWITILTRFKKKYGILDK 229 >ref|NP_781418.1| conserved membrane protein [Clostridium tetani E88] gb|AAO35355.1| conserved membrane protein [Clostridium tetani E88] Length = 228 Score = 119 bits (298), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 55/155 (35%), Positives = 86/155 (55%) Query: 73 RIPFAALPSVKPVSAIVILSAYVFGAEAGFIIGAVAALVSNIYFGQGPWTPWQMFAWGMV 132 R+PF A+P V+PV+ I +S Y G GFI+GA++A +SN + G GPWTPWQM WG+ Sbjct: 71 RVPFTAIPGVQPVTFICAVSGYTLGGINGFIVGAMSAFISNFFIGHGPWTPWQMIGWGLC 130 Query: 133 GLTAGWLRNTWWMKKKGGMLAFGFVWGFLFGWIMNIWYLISLPDAFSWGLVAAAYASSFY 192 GL G R K + +WG+++G+I+N WY++ + + SF Sbjct: 131 GLFFGIFRGKIEKKGIKAFVILCGIWGYIYGFILNQWYILEFITPVTIKTILMGSILSFS 190 Query: 193 LDLAHALSNVFFLAVLAGGWTKVLKRFRKKYGLLQ 227 D HAL N+ F + +VL+R+ K+ +++ Sbjct: 191 HDTLHALGNIIFAKFFGENFIEVLERYNKRNTVIR 225 >ref|ZP_00181173.1| hypothetical protein Mther075101 [Moorella thermoacetica ATCC 39073] Length = 107 Score = 76.6 bits (187), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 40/97 (41%), Positives = 55/97 (56%) Query: 126 MFAWGMVGLTAGWLRNTWWMKKKGGMLAFGFVWGFLFGWIMNIWYLISLPDAFSWGLVAA 185 MFAWG+ G +AG L+ + M F F+WG+LFGWIMN+W +W A Sbjct: 1 MFAWGLAGTSAGVLKIFFPRAGNKVMTVFSFLWGYLFGWIMNLWTWTGFIHPLTWQSFVA 60 Query: 186 AYASSFYLDLAHALSNVFFLAVLAGGWTKVLKRFRKK 222 YA+SF+LD HAL N F + K+L+RFR++ Sbjct: 61 TYAASFWLDTIHALGNAAFYLFFGSSFVKILQRFRRR 97 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.331 0.143 0.490 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 281,579,653 Number of extensions: 10722190 Number of successful extensions: 36581 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 36566 Number of HSP's successfully gapped: 13 Length of query: 228 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 107 Effective length of database: 428,710,599 Effective search space: 45872034093 Effective search space used: 45872034093 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 124 (52.4 bits)