BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0555.0008.p (340 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAL18013.1| dTDP-glucose 4,6-dehydratase [Aneurinibacillus th... 543 e-153 gb|AAR99612.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearot... 540 e-152 gb|AAQ23681.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearot... 518 e-146 gb|AAQ23687.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearot... 506 e-142 ref|NP_276895.1| dTDP-glucose 4,6-dehydratase [Methanothermobact... 490 e-137 >gb|AAL18013.1| dTDP-glucose 4,6-dehydratase [Aneurinibacillus thermoaerophilus] Length = 341 Score = 543 bits (1400), Expect = e-153, Method: Compositional matrix adjust. Identities = 251/336 (74%), Positives = 290/336 (86%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MK+LVTGGAGFIGSNFV YM+ ++P Y +NVDALTYAGNLENLK +EN PNYTF KADI Sbjct: 1 MKVLVTGGAGFIGSNFVNYMVHKYPNYTFVNVDALTYAGNLENLKPVENAPNYTFVKADI 60 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 TD + M+ L + GVDV+VNFAAESHVDRSI EPD FVKTN++GTQ+LLD A+K N+ K+V Sbjct: 61 TDREKMEKLVADGVDVIVNFAAESHVDRSITEPDTFVKTNIMGTQILLDIARKCNINKYV 120 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 Q+STDEVYGTLG TG FTEETPL PNSPYSASKA DLLVRAYHET+GLPVNITRCSNNY Sbjct: 121 QISTDEVYGTLGETGYFTEETPLAPNSPYSASKASADLLVRAYHETYGLPVNITRCSNNY 180 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPY FPEKLIPLMI AL D+ LPVYGDG+NIRDWLYVEDHC+AIDLVIH+G +GEVYN+ Sbjct: 181 GPYHFPEKLIPLMIINALHDKELPVYGDGLNIRDWLYVEDHCAAIDLVIHKGKDGEVYNV 240 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GGNNERTN+ IVK +L+ELGK ++LI +V+DRPGHDRRY ID TK+ ELGW+PK+ FET Sbjct: 241 GGNNERTNIEIVKRILKELGKSENLIRFVKDRPGHDRRYAIDATKLKTELGWQPKYDFET 300 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRL 336 GI +TIQWYL+N++WW RI SG+YQ Y E QY RL Sbjct: 301 GIVKTIQWYLDNRDWWERIISGDYQTYYEQQYKQRL 336 >gb|AAR99612.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearothermophilus] Length = 342 Score = 540 bits (1390), Expect = e-152, Method: Compositional matrix adjust. Identities = 247/339 (72%), Positives = 291/339 (85%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 MK+L+TGGAGFIGSNFV YM+Q++P Y+IIN+DALTYAGNLEN+K +EN PNY F K DI Sbjct: 3 MKVLITGGAGFIGSNFVNYMVQKYPSYQIINLDALTYAGNLENVKEVENEPNYRFVKGDI 62 Query: 61 TDVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFV 120 TD + ++ LF++G+D VVNFAAESHVDRSI +P +F+KTNV GTQVLLDAAKKY V K++ Sbjct: 63 TDRELIESLFAEGIDAVVNFAAESHVDRSIADPGIFIKTNVQGTQVLLDAAKKYGVKKYI 122 Query: 121 QVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNY 180 Q+STDEVYGTLG TG FTE TPL PNSPYSASKAG DLLVRAYHET+GLPVNITRCSNNY Sbjct: 123 QISTDEVYGTLGETGYFTETTPLAPNSPYSASKAGADLLVRAYHETYGLPVNITRCSNNY 182 Query: 181 GPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNI 240 GPY FPEKLIPLMI AL +Q LPVYGDG+N+RDWL+VEDHCSAIDLV+H G NGEVYN+ Sbjct: 183 GPYHFPEKLIPLMIINALNNQPLPVYGDGLNVRDWLHVEDHCSAIDLVLHHGKNGEVYNV 242 Query: 241 GGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFET 300 GGNNERTN+ +VK +L+ L KP+SLIT+V+DR GHDRRY ID TKI ELGW+PK+TFE Sbjct: 243 GGNNERTNIEVVKAILRYLNKPESLITFVEDRLGHDRRYAIDATKIREELGWQPKYTFEE 302 Query: 301 GIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRLGDS 339 G+K+TI WYL N+ WW I SGEY++Y + QYG +LG S Sbjct: 303 GLKQTINWYLENRSWWENILSGEYKEYYKRQYGQKLGRS 341 >gb|AAQ23681.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearothermophilus] Length = 337 Score = 518 bits (1334), Expect = e-146, Method: Compositional matrix adjust. Identities = 243/337 (72%), Positives = 281/337 (83%), Gaps = 1/337 (0%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 M LLVTGGAGFIGSNFV YML+++P YK++N D LTYAGNLENLK +ENHPNYTF K DI Sbjct: 1 MNLLVTGGAGFIGSNFVRYMLEKYPNYKVVNYDLLTYAGNLENLKDVENHPNYTFVKGDI 60 Query: 61 TDVQAMDVLF-SQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKF 119 + + +D L + +DV+VNFAAESHVDRSI +PD+FVKTNVLGTQ LLD AK ++ K+ Sbjct: 61 NNRELVDYLVKTHEIDVIVNFAAESHVDRSITDPDIFVKTNVLGTQALLDVAKANHIKKY 120 Query: 120 VQVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNN 179 VQ+STDEVYGTLG TG FTEETPL PNSPYSASKAGGDLLVRAYHET+GL VNITRCSNN Sbjct: 121 VQISTDEVYGTLGETGYFTEETPLAPNSPYSASKAGGDLLVRAYHETYGLNVNITRCSNN 180 Query: 180 YGPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYN 239 YGPY FPEKLIPLMI+ AL + LP+YGDG NIRDWL+V+DHC+AIDLVIH+G GEVYN Sbjct: 181 YGPYHFPEKLIPLMITNALEGKELPIYGDGQNIRDWLHVKDHCAAIDLVIHKGRPGEVYN 240 Query: 240 IGGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFE 299 IGG+NERTN IV ++++LG LI YV DRPGHDRRY IDPTKI ELGWKP++TFE Sbjct: 241 IGGHNERTNNEIVHLIVEKLGVSKDLIKYVADRPGHDRRYAIDPTKIMTELGWKPQYTFE 300 Query: 300 TGIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRL 336 GI ETIQWY++NQEWW I+SGEY Y + QYG +L Sbjct: 301 KGIAETIQWYIDNQEWWKNIKSGEYMNYYQKQYGDKL 337 >gb|AAQ23687.1| dTDP-glucose 4,6-dehydratase [Geobacillus stearothermophilus] Length = 337 Score = 506 bits (1302), Expect = e-142, Method: Compositional matrix adjust. Identities = 238/336 (70%), Positives = 279/336 (83%), Gaps = 1/336 (0%) Query: 1 MKLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADI 60 M LLVTGGAGFIGSNFV YML ++PEYKI+N D LTYAGNLENLK I+ +P Y F + DI Sbjct: 1 MNLLVTGGAGFIGSNFVRYMLNKYPEYKIVNYDLLTYAGNLENLKDIQENPRYVFVRGDI 60 Query: 61 TDVQAMD-VLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKF 119 + Q +D ++ S +DV+VNFAAESHVDRSI +P VFVKTNVLGTQVLLD AK ++ K+ Sbjct: 61 RNYQLVDYIVKSHHIDVIVNFAAESHVDRSISDPSVFVKTNVLGTQVLLDVAKANSIQKY 120 Query: 120 VQVSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNN 179 VQ+STDEVYG+LG TG FTEETPL PNSPYSASKA DLLVRAYHET+GL VNITRCSNN Sbjct: 121 VQISTDEVYGSLGDTGYFTEETPLAPNSPYSASKASADLLVRAYHETYGLNVNITRCSNN 180 Query: 180 YGPYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYN 239 YGPY FPEKLIPL+I+ AL + LP+YGDG +IRDWLYV+DHC+AIDLVIH+G GEVYN Sbjct: 181 YGPYHFPEKLIPLIITNALEGKELPIYGDGQHIRDWLYVKDHCAAIDLVIHKGKPGEVYN 240 Query: 240 IGGNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFE 299 IGG+NERTN IV ++++LG SLI YV DRPGHDRRY IDPTKI ELGWKP++TF+ Sbjct: 241 IGGHNERTNNEIVHLIVEKLGVSKSLIKYVSDRPGHDRRYAIDPTKIMTELGWKPQYTFD 300 Query: 300 TGIKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSR 335 GI+ETIQWY+NN++WW RI+SGEY Y + QYG R Sbjct: 301 KGIEETIQWYINNKDWWKRIKSGEYMAYYQKQYGER 336 >ref|NP_276895.1| dTDP-glucose 4,6-dehydratase [Methanothermobacter thermautotrophicus] pir||H69105 dTDP-glucose 4,6-dehydratase - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB86255.1| dTDP-glucose 4,6-dehydratase [Methanothermobacter thermautotrophicus str. Delta H] Length = 336 Score = 490 bits (1261), Expect = e-137, Method: Compositional matrix adjust. Identities = 223/337 (66%), Positives = 280/337 (83%), Gaps = 3/337 (0%) Query: 2 KLLVTGGAGFIGSNFVIYMLQQHPEYKIINVDALTYAGNLENLKSIENHPNYTFAKADIT 61 K+LVTGGAGFIGSNF+ YMLQ+HP Y IIN+DALTY GNLENL+ +E+ P YTF + IT Sbjct: 3 KILVTGGAGFIGSNFIRYMLQEHP-YHIINLDALTYCGNLENLRGVEDEPRYTFVRGSIT 61 Query: 62 DVQAMDVLFSQGVDVVVNFAAESHVDRSILEPDVFVKTNVLGTQVLLDAAKKYNVTKFVQ 121 D + +D + + VD V+NFAAESHVDRSI +P++F++TN+LGTQ LL+A++K+ V +F+Q Sbjct: 62 DRKLVDGII-KDVDAVINFAAESHVDRSIEDPEIFIRTNILGTQTLLEASRKHGVERFIQ 120 Query: 122 VSTDEVYGTLGATGLFTEETPLTPNSPYSASKAGGDLLVRAYHETFGLPVNITRCSNNYG 181 +STDEVYG+ G FTEETPL PNSPYSASKA DL+VRAYH T+GLPVNITRCSNNYG Sbjct: 121 ISTDEVYGS-AEKGYFTEETPLAPNSPYSASKASADLMVRAYHRTYGLPVNITRCSNNYG 179 Query: 182 PYQFPEKLIPLMISRALADQALPVYGDGMNIRDWLYVEDHCSAIDLVIHEGVNGEVYNIG 241 PYQFPEKLIPLMI+ AL ++ LPVYGDGMN+RDW++V DHC A+DLV+H G GEVYNIG Sbjct: 180 PYQFPEKLIPLMITNALENKPLPVYGDGMNVRDWIHVLDHCRAVDLVLHRGRVGEVYNIG 239 Query: 242 GNNERTNVHIVKTVLQELGKPDSLITYVQDRPGHDRRYGIDPTKITRELGWKPKHTFETG 301 GN+ER N+ IV+ +++ELGK +SLI +V+DRPGHDRRY ID +KI ELGWKP ++FE G Sbjct: 240 GNSERRNIEIVELIVRELGKDESLIRFVEDRPGHDRRYAIDASKIRNELGWKPLYSFEEG 299 Query: 302 IKETIQWYLNNQEWWTRIQSGEYQKYAELQYGSRLGD 338 I+ETI+WY++N++WW I+SGEY +Y E YG RL D Sbjct: 300 IRETIRWYIDNRDWWENIKSGEYLRYYERMYGGRLQD 336 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 531,549,888 Number of extensions: 22565319 Number of successful extensions: 72289 Number of sequences better than 1.0e-05: 3795 Number of HSP's gapped: 66790 Number of HSP's successfully gapped: 3807 Length of query: 340 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 214 Effective length of database: 415,135,104 Effective search space: 88838912256 Effective search space used: 88838912256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)