BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0010.p (462 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I40794 dihydrolipoamide dehydrogenase (EC 1.8.1.4) [validat... 491 e-137 ref|NP_242691.1| acetoin dehydrogenase E3 component (dihydrolipo... 389 e-107 ref|ZP_00018092.1| hypothetical protein [Chloroflexus aurantiacus] 369 e-100 ref|NP_175237.1| dihydrolipoamide dehydrogenase 1, mitochondrial... 350 3e-95 pir||F96520 dihydrolipoamide dehydrogenase (EC 1.8.1.4) [similar... 350 4e-95 >pir||I40794 dihydrolipoamide dehydrogenase (EC 1.8.1.4) [validated] - Clostridium magnum gb|AAA21748.1| dihydrolipoamide dehydrogenase Length = 578 Score = 491 bits (1263), Expect = e-137, Method: Compositional matrix adjust. Identities = 259/460 (56%), Positives = 332/460 (72%), Gaps = 2/460 (0%) Query: 2 RIVVLGGGPGGYVAAIRAAQLGAEVTLVEKKALGGTCLNVGCIPTKVLLHTTELYTHLKR 61 +VV+GGGPGGYVAAIRAAQLGA+VTL+EK++LGGTCLNVGCIPTKVLLH+++L T +K Sbjct: 117 NLVVIGGGPGGYVAAIRAAQLGAKVTLIEKESLGGTCLNVGCIPTKVLLHSSQLLTEMK- 175 Query: 62 EGKALGIE-AADVSVNWGQLQKRKDKIIRMNTGGIEALLTKNKVARLSGSGKFISARELE 120 EG LGI+ + VNW +QKRK +I+ G+ LLT NKV + G+ KF S + Sbjct: 176 EGDKLGIDIEGSIVVNWKHIQKRKKIVIKKLVSGVSGLLTCNKVKVIKGTAKFESKDTIL 235 Query: 121 VTAADGSVRTVGFDRAIIATGSEPSSVPIPGAGLEGVITSDGALSLPAVPPSLCIIGGGV 180 VT DG V FD AIIATGS P I G L GVI S GALSL + P S+ IIGGGV Sbjct: 236 VTKEDGVAEKVNFDNAIIATGSMPFIPEIEGNKLSGVIDSTGALSLESNPESIAIIGGGV 295 Query: 181 IGCEFASVYSSLGCKVTVIEMLPELIAAMDGDIVQCLRKEFASSGIEVHTETKVERIEQT 240 IG EFAS+++SLGCKV++IEMLP ++ MD +I + + + GI ++ KV RIEQ Sbjct: 296 IGVEFASIFNSLGCKVSIIEMLPHILPPMDREISEIAKAKLIRDGININNNCKVTRIEQG 355 Query: 241 AAGLRVVTSSGNGSHVIEADKVLLSIGRRPVTAGLGLEDIGVQLTRGAIQVNRKMETCIP 300 GL+V G I+ +KVL+++GRR GL +E IGV+ G+I VN KMET + Sbjct: 356 EDGLKVSFIGDKGEESIDVEKVLIAVGRRSNIEGLDVEKIGVKTEGGSIIVNDKMETNVE 415 Query: 301 GIYAIGDCNGGIMLAHVASAEGVIAAEAIMGEPSPVDFRTVPSCVYTRPELASVGMTETE 360 GIYAIGDC G IMLAHVAS +GV+AAE IMG+ +D++TVP+CVYT+PELASVG+TE + Sbjct: 416 GIYAIGDCTGKIMLAHVASDQGVVAAENIMGQNKKMDYKTVPACVYTKPELASVGLTEEQ 475 Query: 361 AKQQGFRVKTGLFPLQANAKSMIMGESSGLVKYVVDAGNDEILGLHIAGPRATDIIVEGA 420 AK++G K G F L AN KS+IM E+ G++K + D +EILG+HI GPRATD+I E A Sbjct: 476 AKEKGIDYKVGKFQLAANGKSLIMNETGGVIKIITDKKYEEILGVHILGPRATDLITEAA 535 Query: 421 LAIRLEATLEEVVTTIHAHPTVAESLHEAAHAVHGRAIHL 460 LA+RLEATLEE++TT+HAHPTV E++ EAA AV+ +AIH+ Sbjct: 536 LALRLEATLEEIITTVHAHPTVGEAMKEAALAVNNQAIHM 575 >ref|NP_242691.1| acetoin dehydrogenase E3 component (dihydrolipoamide dehydroge) [Bacillus halodurans] pir||A83878 acetoin dehydrogenase E3 component (dihydrolipoamide dehydroge) acoL [imported] - Bacillus halodurans (strain C-125) dbj|BAB05544.1| acetoin dehydrogenase E3 component (dihydrolipoamide dehydroge) [Bacillus halodurans] Length = 459 Score = 389 bits (1000), Expect = e-107, Method: Compositional matrix adjust. Identities = 208/461 (45%), Positives = 293/461 (63%), Gaps = 6/461 (1%) Query: 2 RIVVLGGGPGGYVAAIRAAQLGAEVTLVEKKALGGTCLNVGCIPTKVLLHTTELYTHLKR 61 R+ ++GGGP GYVAAI AA G +VTL+E+K LGGTCLN GC+PTK LL + Y +K Sbjct: 3 RVAIVGGGPAGYVAAITAAHRGKQVTLIEQKKLGGTCLNEGCMPTKSLLESAATYDKVKH 62 Query: 62 EGKALGIEAA--DVSVNWGQLQKRKDKIIRMNTGGIEALLTKNKVARLSGSGKFISAREL 119 GI+ ++ + WGQ+Q RK++I+ GI+ LL K+K+ L G+ F SA EL Sbjct: 63 -ASDYGIKGPVDEMRIEWGQVQARKNQIVHQLVKGIDYLLKKHKIELLEGTASFQSAHEL 121 Query: 120 EVTAADGSVRTVGFDRAIIATGSEPSSVPIPGAGLEGVITSDGALSLPAVPPSLCIIGGG 179 V G +T+ +R IIATGSEP+ +P E VI+S+ A+SLP P S+ I+GGG Sbjct: 122 LVQTERGQ-QTIEANRIIIATGSEPTPLPFAPFDREWVISSEQAMSLPIQPESMIIVGGG 180 Query: 180 VIGCEFASVYSSLGCKVTVIEMLPELIAAMDGDIVQCLRKEFASSGIEVHTETKVERIEQ 239 VIGCEFAS+Y +G KVT+IEM L+ D DI + L +E G+E++ T ++ +++ Sbjct: 181 VIGCEFASIYGRMGTKVTIIEMADRLLFGEDHDICKVLMEELKRQGVEIYPSTVIKEMQR 240 Query: 240 TAAGLRVVTSSGNGSHVIEADKVLLSIGRRPVTAGLGLEDIGVQLTRGAIQVNRKMETCI 299 +VV V+ D VL++IGR+P +GLE IGV T I VN M+T I Sbjct: 241 DRK--QVVVERDGQVEVLTGDYVLVAIGRKPRLQEIGLERIGVDFTSRGITVNEHMQTTI 298 Query: 300 PGIYAIGDCNGGIMLAHVASAEGVIAAEAIMGEPSPVDFRTVPSCVYTRPELASVGMTET 359 P IYA GD GGI LAHVA EG +AA+ + GEP V + VP C+YT PE+A+VG+TET Sbjct: 299 PHIYACGDVIGGIQLAHVAFHEGSVAAKHLCGEPIQVRYEAVPRCIYTSPEIATVGLTET 358 Query: 360 EAKQQGFRVKTGLFPLQANAKSMIMGESSGLVKYVVDAGNDEILGLHIAGPRATDIIVEG 419 +A++ V+ G +P AN K++I+ E+ G VK VV+ + I+GL I GP AT++I +G Sbjct: 359 QARENYGHVRIGEYPFAANGKALIVNETIGKVKVVVEPEYEAIVGLTIVGPHATELIAQG 418 Query: 420 ALAIRLEATLEEVVTTIHAHPTVAESLHEAAHAVHGRAIHL 460 L + E TL+ + + I AHPTV+E++HEA G+A+H+ Sbjct: 419 TLMVHNELTLDSLESFIAAHPTVSEAVHEAVLQALGQAVHV 459 >ref|ZP_00018092.1| hypothetical protein [Chloroflexus aurantiacus] Length = 470 Score = 369 bits (946), Expect = e-100, Method: Compositional matrix adjust. Identities = 208/467 (44%), Positives = 292/467 (62%), Gaps = 13/467 (2%) Query: 3 IVVLGGGPGGYVAAIRAAQLGAEVTLVEKKALGGTCLNVGCIPTKVLLHTTELYTHLKRE 62 +VVLG GPGGYVAAIRAAQLG + +VE ALGG CLN+GCIPTK LLH+ +L +K E Sbjct: 8 LVVLGSGPGGYVAAIRAAQLGMKTAIVEVNALGGVCLNIGCIPTKALLHSADLLEEVK-E 66 Query: 63 GKALGIEAADVSVNWGQLQKRKDKIIRMNTGGIEALLTKNKVARLSGSGKFISARELEVT 122 K GI +V+ K KD +++ +T G+ L+ KNK+ ++G G+ I ++ V Sbjct: 67 AKRFGITVENVAFELAGAMKHKDTVVKQSTDGVAFLMKKNKIEVVAGRGRLIGRGQVHVQ 126 Query: 123 AADGSVRTVGFDRAIIATGSEPSSVPIPGAGLEG--VITSDGALSLPAVPPSLCIIGGGV 180 +G R + I+ATG P P PG +G V++S L+L VP SL IG G Sbjct: 127 LNEGGERVLAAKHIIVATGGRPR--PFPGIPFDGERVLSSTDMLTLKTVPSSLLAIGAGA 184 Query: 181 IGCEFASVYSSLGCKVTVIEMLPELIAAMDGDIVQCLRKEFASSGIEVHTETKVERIEQT 240 IG EFAS++ S G VT++E LP ++ D ++ L K F GI+ KVE ++ Sbjct: 185 IGVEFASMFRSFGSDVTIVEALPRIVPNEDEEVSAELTKAFQRRGIKTLAGAKVEGVDVG 244 Query: 241 AAG--LRVVTSSGNGSHVIEADKVLLSIGRRPVTAGLGLEDIGVQL-TRGAIQVNRKMET 297 + VV SSG I +K+L+SIG P T +GLE++GV++ RG I+ + + T Sbjct: 245 GEKVVVTVVDSSGKPQQ-IAVEKLLVSIGIAPNTENIGLEEVGVKVNNRGFIETDGFLRT 303 Query: 298 CIPGIYAIGDCNGGI-MLAHVASAEGVIAAEAIMGEP-SPVDFRTVPSCVYTRPELASVG 355 G+YAIGDC LAH ASAEG++AAE I G +P+D+ + +C Y PE+ASVG Sbjct: 304 SAEGVYAIGDCTANTPWLAHKASAEGILAAEHIAGHHVTPIDYGKIAACTYCNPEIASVG 363 Query: 356 MTETEAKQQGFRVKTGLFPLQANAKSMIMGESS-GLVKYVVDAGNDEILGLHIAGPRATD 414 +TE +A+++G++VK G FP AN K+ ++G++ G +K V DA DEILG+H+ GPR T+ Sbjct: 364 LTEAKARERGYQVKVGKFPFSANGKARVLGQTRFGFIKLVADAQYDEILGVHMIGPRVTE 423 Query: 415 IIVEGALAIRLEATLEEVVTTIHAHPTVAESLHEAAHA-VHGRAIHL 460 +I EG +A+ EAT E ++ TIHAHPT+ E++ EAAHA VHG IHL Sbjct: 424 MIAEGGIALSHEATGESMMQTIHAHPTLYEAIGEAAHALVHGAPIHL 470 >ref|NP_175237.1| dihydrolipoamide dehydrogenase 1, mitochondrial / lipoamide dehydrogenase 1 (MTLPD1) [Arabidopsis thaliana] ref|NP_849782.1| dihydrolipoamide dehydrogenase 1, mitochondrial / lipoamide dehydrogenase 1 (MTLPD1) [Arabidopsis thaliana] gb|AAG51522.1| lipoamide dehydrogenase, putative; 44693-46402 [Arabidopsis thaliana] gb|AAF34795.3| lipoamide dehydrogenase precursor [Arabidopsis thaliana] Length = 507 Score = 350 bits (899), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 197/462 (42%), Positives = 285/462 (61%), Gaps = 4/462 (0%) Query: 3 IVVLGGGPGGYVAAIRAAQLGAEVTLVEKK-ALGGTCLNVGCIPTKVLLHTTELYTHLKR 61 +V++GGGPGGYVAAI+A+QLG + T +EK+ ALGGTCLNVGCIP+K LLH++ +Y K Sbjct: 46 VVIIGGGPGGYVAAIKASQLGLKTTCIEKRGALGGTCLNVGCIPSKALLHSSHMYHEAKH 105 Query: 62 EGKALGIEAADVSVNWGQLQKRKDKIIRMNTGGIEALLTKNKVARLSGSGKFISARELEV 121 GI+ + V V+ + +KD ++ T GIE L KNKV + G GKFIS E+ V Sbjct: 106 SFANHGIKVSSVEVDLPAMLAQKDNAVKNLTRGIEGLFKKNKVTYVKGYGKFISPNEVSV 165 Query: 122 TAADGSVRTVGFDRAIIATGSEPSSVPIPGAGLEGVITSDGALSLPAVPPSLCIIGGGVI 181 DG V I+ATGS+ S+P + +++S GALSL VP L +IG G I Sbjct: 166 ETIDGGNTIVKGKHIIVATGSDVKSLPGITIDEKKIVSSTGALSLSEVPKKLIVIGAGYI 225 Query: 182 GCEFASVYSSLGCKVTVIEMLPELIAAMDGDIVQCLRKEFASSGIEVHTETKVERIEQTA 241 G E SV+ LG +VTV+E +++ +MDG+I + ++ ++ +TKV ++ ++ Sbjct: 226 GLEMGSVWGRLGSEVTVVEFAGDIVPSMDGEIRKQFQRSLEKQKMKFMLKTKVVSVDSSS 285 Query: 242 AGLRVVTSSGNG--SHVIEADKVLLSIGRRPVTAGLGLEDIGVQLTR-GAIQVNRKMETC 298 G+++ G ++EAD VL+S GR P T+GL LE IGV+ + G I VN + + Sbjct: 286 DGVKLTVEPAEGGEQSILEADVVLVSAGRTPFTSGLDLEKIGVETDKAGRILVNDRFLSN 345 Query: 299 IPGIYAIGDCNGGIMLAHVASAEGVIAAEAIMGEPSPVDFRTVPSCVYTRPELASVGMTE 358 +PG+YAIGD G MLAH A +GV E I G+ VD+ VP VYT PE+ASVG TE Sbjct: 346 VPGVYAIGDVIPGPMLAHKAEEDGVACVEFIAGKHGHVDYDKVPGVVYTHPEVASVGKTE 405 Query: 359 TEAKQQGFRVKTGLFPLQANAKSMIMGESSGLVKYVVDAGNDEILGLHIAGPRATDIIVE 418 + K++G + G FP AN+++ + + GLVK + D D+ILG+HI P A ++I E Sbjct: 406 EQLKKEGVSYRVGKFPFMANSRAKAIDNAEGLVKILADKETDKILGVHIMAPNAGELIHE 465 Query: 419 GALAIRLEATLEEVVTTIHAHPTVAESLHEAAHAVHGRAIHL 460 LAI +A+ E++ HAHPT++E+L EAA A + + IH+ Sbjct: 466 AVLAINYDASSEDIARVCHAHPTMSEALKEAAMATYDKPIHI 507 >pir||F96520 dihydrolipoamide dehydrogenase (EC 1.8.1.4) [similarity] - Arabidopsis thaliana gb|AAF79529.1| F21D18.28 [Arabidopsis thaliana] Length = 505 Score = 350 bits (898), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 197/462 (42%), Positives = 285/462 (61%), Gaps = 4/462 (0%) Query: 3 IVVLGGGPGGYVAAIRAAQLGAEVTLVEKK-ALGGTCLNVGCIPTKVLLHTTELYTHLKR 61 +V++GGGPGGYVAAI+A+QLG + T +EK+ ALGGTCLNVGCIP+K LLH++ +Y K Sbjct: 44 VVIIGGGPGGYVAAIKASQLGLKTTCIEKRGALGGTCLNVGCIPSKALLHSSHMYHEAKH 103 Query: 62 EGKALGIEAADVSVNWGQLQKRKDKIIRMNTGGIEALLTKNKVARLSGSGKFISARELEV 121 GI+ + V V+ + +KD ++ T GIE L KNKV + G GKFIS E+ V Sbjct: 104 SFANHGIKVSSVEVDLPAMLAQKDNAVKNLTRGIEGLFKKNKVTYVKGYGKFISPNEVSV 163 Query: 122 TAADGSVRTVGFDRAIIATGSEPSSVPIPGAGLEGVITSDGALSLPAVPPSLCIIGGGVI 181 DG V I+ATGS+ S+P + +++S GALSL VP L +IG G I Sbjct: 164 ETIDGGNTIVKGKHIIVATGSDVKSLPGITIDEKKIVSSTGALSLSEVPKKLIVIGAGYI 223 Query: 182 GCEFASVYSSLGCKVTVIEMLPELIAAMDGDIVQCLRKEFASSGIEVHTETKVERIEQTA 241 G E SV+ LG +VTV+E +++ +MDG+I + ++ ++ +TKV ++ ++ Sbjct: 224 GLEMGSVWGRLGSEVTVVEFAGDIVPSMDGEIRKQFQRSLEKQKMKFMLKTKVVSVDSSS 283 Query: 242 AGLRVVTSSGNG--SHVIEADKVLLSIGRRPVTAGLGLEDIGVQLTR-GAIQVNRKMETC 298 G+++ G ++EAD VL+S GR P T+GL LE IGV+ + G I VN + + Sbjct: 284 DGVKLTVEPAEGGEQSILEADVVLVSAGRTPFTSGLDLEKIGVETDKAGRILVNDRFLSN 343 Query: 299 IPGIYAIGDCNGGIMLAHVASAEGVIAAEAIMGEPSPVDFRTVPSCVYTRPELASVGMTE 358 +PG+YAIGD G MLAH A +GV E I G+ VD+ VP VYT PE+ASVG TE Sbjct: 344 VPGVYAIGDVIPGPMLAHKAEEDGVACVEFIAGKHGHVDYDKVPGVVYTHPEVASVGKTE 403 Query: 359 TEAKQQGFRVKTGLFPLQANAKSMIMGESSGLVKYVVDAGNDEILGLHIAGPRATDIIVE 418 + K++G + G FP AN+++ + + GLVK + D D+ILG+HI P A ++I E Sbjct: 404 EQLKKEGVSYRVGKFPFMANSRAKAIDNAEGLVKILADKETDKILGVHIMAPNAGELIHE 463 Query: 419 GALAIRLEATLEEVVTTIHAHPTVAESLHEAAHAVHGRAIHL 460 LAI +A+ E++ HAHPT++E+L EAA A + + IH+ Sbjct: 464 AVLAINYDASSEDIARVCHAHPTMSEALKEAAMATYDKPIHI 505 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 649,201,857 Number of extensions: 26909686 Number of successful extensions: 104566 Number of sequences better than 1.0e-05: 2628 Number of HSP's gapped: 97522 Number of HSP's successfully gapped: 2639 Length of query: 462 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 333 Effective length of database: 406,989,807 Effective search space: 135527605731 Effective search space used: 135527605731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)