BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0009.p (433 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I40793 dihydrolipoamide S-acetyltransferase (EC 2.3.1.12) -... 431 e-119 ref|NP_241644.1| dihydrolipoamide S-acetyltransferase [Bacillus ... 247 3e-64 ref|NP_720602.1| putative dihydrolipoamide acetyltransferase [St... 245 1e-63 ref|NP_687894.1| acetoin dehydrogenase, thymine PPi dependent, E... 227 4e-58 ref|NP_623265.1| Dihydrolipoamide acyltransferases [Thermoanaero... 224 3e-57 >pir||I40793 dihydrolipoamide S-acetyltransferase (EC 2.3.1.12) - Clostridium magnum gb|AAA21747.1| dihydrolipoamide acetyltransferase Length = 443 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 224/436 (51%), Positives = 286/436 (65%), Gaps = 9/436 (2%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEG-TVGIFQPV 59 MTEGT+ W+KAEGD V GEI F+V TDK++NEVEA G++RK++V EG TV +PV Sbjct: 14 MTEGTLVTWKKAEGDQVKVGEIFFEVSTDKLTNEVEASDEGIVRKLLVNEGDTVECLKPV 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 A+IG A+EDIS+LL ++ ASP AKK A Sbjct: 74 AIIGSADEDISSLLNGSSEGSESAEQNDTKAPKKEAEAPKGAVEKQQGKVKASPAAKKLA 133 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 A ++++ V G+GPQGRIT DV K+ + + L +D S + Sbjct: 134 AENNIDITLVEGTGPQGRITTEDVEKYIEDSKNASKASPMASKVAAE----LNVDLSTIE 189 Query: 180 KEGRIMKEDIRSFAA--ASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPA 237 K+GRIMKED+ S A + P+ +++ VPM +R+II+ RM ES SP Sbjct: 190 KDGRIMKEDVLSLCKGNAPEECKVNPSEDKYTEKI--VPMTQIRKIISARMHESWITSPT 247 Query: 238 VTYNMKLDTTALGLLRQQLKDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVLRNYA 297 VTY++K+D T+L + LKD KVTYTDL+V I +KVLL+FPLLNCS+ G EL+ RNY Sbjct: 248 VTYDIKVDMTSLKRFKDALKDVCKVTYTDLIVKIVSKVLLQFPLLNCSINGNELITRNYV 307 Query: 298 NIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTITNLG 357 N+GVAV ++ GL+VPVVK A +KGL EIS EVK LA A++N L PE +TGGTFTITNLG Sbjct: 308 NMGVAVAIDGGLVVPVVKYANEKGLKEISTEVKDLAKKAKSNQLKPENMTGGTFTITNLG 367 Query: 358 MYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAVAAQF 417 M+G+E FSPIINQPEVAILGVN I +TPV NGEIVI+PLMNLSLTA+HRAVDG+VAAQF Sbjct: 368 MFGIEYFSPIINQPEVAILGVNKITETPVVQNGEIVIKPLMNLSLTANHRAVDGSVAAQF 427 Query: 418 MQKIKAYAENPALLLL 433 + K+K Y E P LL+L Sbjct: 428 LSKVKEYMEKPELLML 443 >ref|NP_241644.1| dihydrolipoamide S-acetyltransferase [Bacillus halodurans] pir||B83747 dihydrolipoamide S-acetyltransferase BH0778 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04497.1| dihydrolipoamide S-acetyltransferase [Bacillus halodurans] Length = 436 Score = 247 bits (631), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 148/440 (33%), Positives = 233/440 (52%), Gaps = 24/440 (5%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEA-KLGGVLRKIIVEEGTVGIFQPV 59 M EGT+ W K EGD V GE LF++ TDKI+ EVEA + G +L++ E+ + + + Sbjct: 14 MQEGTLLQWFKEEGDRVEVGEPLFEIMTDKINIEVEAYEEGTLLKRYYGEDDEIPVNHVI 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 IG +E + + A+P A++ A Sbjct: 74 GYIGTPDESVPT---EPPGASEITASSTDEAGDHRTTAVKKAPSSDRENVRATPAARRIA 130 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSGLH 179 ++L+QV GSGP+GR+ A DV +G + G Sbjct: 131 KEKRIDLRQVEGSGPEGRVQAVDVATFKKKGQKATPLAKKVAEVKGVALEKV----QGSG 186 Query: 180 KEGRIMKEDIRSFAAASTKTTAQPAVQAAIQEVTRVPMNSMRRIIAKRMLESQAVSPAVT 239 G++ +ED+ AAS P RV ++ +R+++AKRM++S +P VT Sbjct: 187 PYGKVYREDVEHAQAAS------PVEDKG----NRVKLSGLRKVVAKRMVDSAFSAPHVT 236 Query: 240 YNMKLDTTALGLLRQQL------KDTLKVTYTDLLVSIAAKVLLEFPLLNCSVEGTELVL 293 ++D ++ +R QL + +++YT++++ A L+ P +N S E+V Sbjct: 237 ITTEIDMSSTIKIRSQLLGMIEQETGYRLSYTEIVMKAVAHALMSHPTINASFFENEIVY 296 Query: 294 RNYANIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLTPEELTGGTFTI 353 +IG+AV +E GL+VPVVK+ +KGLA+++ E K +A AR+N L+ E ++GGTFTI Sbjct: 297 HEDVHIGLAVAVEGGLVVPVVKHVDKKGLAQLTNECKTVAMAARDNRLSQEMMSGGTFTI 356 Query: 354 TNLGMYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSLTADHRAVDGAV 413 +NLGMY ++ F+P+INQPE AILGV I + PV ++G+I +RP+M SL+ DHR +DGA Sbjct: 357 SNLGMYAIDVFTPVINQPESAILGVGRIQEKPVGIDGQIELRPMMTASLSFDHRVIDGAP 416 Query: 414 AAQFMQKIKAYAENPALLLL 433 AA F+ +K+ E P LL+ Sbjct: 417 AAAFLTDVKSMLEQPFQLLM 436 >ref|NP_720602.1| putative dihydrolipoamide acetyltransferase [Streptococcus mutans UA159] gb|AAN57908.1| putative dihydrolipoamide acetyltransferase [Streptococcus mutans UA159] Length = 455 Score = 245 bits (626), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 166/451 (36%), Positives = 234/451 (51%), Gaps = 27/451 (5%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEG-TVGIFQPV 59 M EG I W+K EGD V +GEIL ++ +DK + E+EA+ GVL KI+ G V + + + Sbjct: 14 MQEGEIIEWKKQEGDEVKEGEILLEIMSDKTNMEIEAEDSGVLLKIVKGNGQVVPVTEVI 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIIASPYAKKTA 119 IG A E + + +I A+P A+K A Sbjct: 74 GYIGQAGEVLE--IADVPASTVPKENSAAPAEKTKAMSSPTVAAAPQGKIRATPAARKAA 131 Query: 120 ALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGID---PS 176 L V L QV G+G +GR+ DV A GID S Sbjct: 132 RDLGVNLNQVSGTGAKGRVHKEDVESFKA-------AQPKATPLARKIAIDKGIDLASVS 184 Query: 177 GLHKEGRIMKEDIRSF--AAASTKTTAQPAVQAAI--QEVTRVPMNSMRRIIAKRMLESQ 232 G G+I+KEDI + AA + PA +AA + V + M++MR+ +AK M+ S Sbjct: 185 GTGFGGKIIKEDILNLFEAAQPVNDVSDPAKEAAALPEGVEVIKMSAMRKAVAKSMVNSY 244 Query: 233 AVSPAVTYNMKLDTTALGLLRQQLKDTL------KVTYTDLLVSIAAKVLL--EFPLLNC 284 +P T N +D T + LR++L D + KV++TDL+ K L+ E LN Sbjct: 245 LTAPTFTLNYDIDMTEMIALRKKLIDPIMEKTGFKVSFTDLIGLAVVKTLMKPEHRYLNA 304 Query: 285 SV--EGTELVLRNYANIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNSLT 342 S+ + TE+ L + N+G+AVGL+EGL+VPVV A + L++ K + A+ L Sbjct: 305 SLINDATEIELHQFVNLGIAVGLDEGLLVPVVHGADKMSLSDFVIASKDVIKKAQTGKLK 364 Query: 343 PEELTGGTFTITNLGMYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNLSL 402 E++G TF+ITNLGM+G ++F+PIINQP AILGV A + TP V+GEI IRP+M L L Sbjct: 365 ATEMSGSTFSITNLGMFGTKTFNPIINQPNSAILGVGATIQTPTVVDGEIKIRPIMALCL 424 Query: 403 TADHRAVDGAVAAQFMQKIKAYAENPALLLL 433 T DHR VDG A+FM +K ENP LL+ Sbjct: 425 TIDHRLVDGMNGAKFMVDLKKLMENPFTLLI 455 >ref|NP_687894.1| acetoin dehydrogenase, thymine PPi dependent, E2 component, dihydrolipoamide acetyltransferase [Streptococcus agalactiae 2603V/R] gb|AAM99766.1| acetoin dehydrogenase, thymine PPi dependent, E2 component, dihydrolipoamide acetyltransferase [Streptococcus agalactiae 2603V/R] Length = 462 Score = 227 bits (579), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 156/453 (34%), Positives = 230/453 (50%), Gaps = 24/453 (5%) Query: 1 MTEGTISNWRKAEGDTVTQGEILFDVETDKISNEVEAKLGGVLRKIIVEEG-TVGIFQPV 59 M EG I W+K GD V +G++L ++ +DK + E+EA+ GVL KI G V + + + Sbjct: 14 MQEGEILEWKKQVGDVVNEGDVLLEIMSDKTNMEIEAEDSGVLLKITHGNGDVVPVTETI 73 Query: 60 AVIGDANEDISALLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--RIIASPYAKK 117 IG E+++ ++ A+P A+K Sbjct: 74 GYIGAEGEEVTEASSSENTSVEENATQVTSEPEKVEETSEPSVPAATSGEKVRATPAARK 133 Query: 118 TAALLSVELQQVRGSGPQGRITAGDVRKHAAEGGSGXXXXXXXXXXXXXXXXXLGIDPSG 177 A +S++L V G+G GR+ DV +G I SG Sbjct: 134 LAREMSIDLALVSGTGANGRVHREDVENF--KGAQPRITPLARRIAEDQGVDIAEITGSG 191 Query: 178 LHKEGRIMKEDIRSF-----AAASTKTTAQPAVQAA--IQEVTRVPMNSMRRIIAKRMLE 230 + G+I+K D+ + A A +T A P + + V + M++MR+ I+K M Sbjct: 192 IR--GKIVKNDVLAAMSPQAAEAPVETKATPTTEEKQLPEGVEVIKMSAMRKAISKGMTN 249 Query: 231 SQAVSPAVTYNMKLDTTALGLLRQQLKDT------LKVTYTDLLVSIAAKVLL--EFPLL 282 S +P+ T N +D T + LR++L D LKV++TDL+ K L+ E L Sbjct: 250 SYLTAPSFTLNYDIDMTEMMALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYL 309 Query: 283 NCSV--EGTELVLRNYANIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAAGARNNS 340 N S+ + E+ L N+ NIG+AVGL++GLIVPVV NA Q L++ K + + Sbjct: 310 NASLINDAQEIELHNFVNIGIAVGLDDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGK 369 Query: 341 LTPEELTGGTFTITNLGMYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVIRPLMNL 400 L E++G TF+ITNLGM+G ++F+PIINQP AILGV A + TP V+GEIV RP+M + Sbjct: 370 LKSAEMSGSTFSITNLGMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAM 429 Query: 401 SLTADHRAVDGAVAAQFMQKIKAYAENPALLLL 433 LT DHR VDG A+FM +K ENP LL+ Sbjct: 430 CLTIDHRIVDGMNGAKFMVDLKNLMENPFGLLI 462 >ref|NP_623265.1| Dihydrolipoamide acyltransferases [Thermoanaerobacter tengcongensis] gb|AAM24869.1| Dihydrolipoamide acyltransferases [Thermoanaerobacter tengcongensis] Length = 219 Score = 224 bits (572), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 114/219 (52%), Positives = 150/219 (68%), Gaps = 2/219 (0%) Query: 217 MNSMRRIIAKRMLESQAVSPAVTYNMKLDTTALGLLRQQLKDT--LKVTYTDLLVSIAAK 274 M++MRR I++RM +S P VT ++K+D T L LR+ L + K TYTDL+ Sbjct: 1 MDTMRRTISQRMKKSWTEIPHVTEDIKVDVTELVNLRENLNKSGEHKFTYTDLIAKACVI 60 Query: 275 VLLEFPLLNCSVEGTELVLRNYANIGVAVGLEEGLIVPVVKNAAQKGLAEISQEVKRLAA 334 L + P+LN S+EG E++ N+G+AV LE+GLIVPVVKNA K L E+S+E+K L+ Sbjct: 61 ALKKNPVLNWSIEGDEIIKNPNINLGIAVALEDGLIVPVVKNAENKSLLELSKEIKELSE 120 Query: 335 GARNNSLTPEELTGGTFTITNLGMYGMESFSPIINQPEVAILGVNAIVDTPVAVNGEIVI 394 AR N LTP+E+TGGTFTITNLGMY ++SF+PIIN PE AILGVN I PV + IVI Sbjct: 121 KARENKLTPDEITGGTFTITNLGMYEIDSFTPIINPPESAILGVNKIYKEPVVIEDNIVI 180 Query: 395 RPLMNLSLTADHRAVDGAVAAQFMQKIKAYAENPALLLL 433 R M LSL+ DHR +DGA AA+F+ +K ENP +L+ Sbjct: 181 RHTMKLSLSFDHRLIDGATAAKFLLDLKKILENPVSMLI 219 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 500,494,998 Number of extensions: 17801203 Number of successful extensions: 50820 Number of sequences better than 1.0e-05: 1535 Number of HSP's gapped: 49381 Number of HSP's successfully gapped: 1666 Length of query: 433 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 304 Effective length of database: 406,989,807 Effective search space: 123724901328 Effective search space used: 123724901328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)