BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0554.0008.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I40791 acetoin dehydrogenase (TPP-dependent) (EC 1.-.-.-) b... 405 e-112 ref|NP_879468.1| putative pyruvate dehydrogenase E1 beta subunit... 336 5e-91 ref|NP_241643.1| acetoin dehydrogenase (TPP-dependent) beta chai... 335 6e-91 ref|NP_891237.1| putative pyruvate dehydrogenase E1 beta subunit... 335 1e-90 ref|NP_342959.1| Pyruvate dehydrogenase, beta subunit (lipoamide... 324 1e-87 >pir||I40791 acetoin dehydrogenase (TPP-dependent) (EC 1.-.-.-) beta chain - Clostridium magnum gb|AAA21745.1| TPP-dependent acetoin dehydrogenase beta-subunit Length = 333 Score = 405 bits (1041), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/333 (59%), Positives = 252/333 (75%), Gaps = 10/333 (3%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M+ MTY E +RE MR KM ED V++LGEDVG +GG FG+T GL++EFG++RV+DTPISE Sbjct: 1 MKTMTYMEALREAMRIKMKEDEKVLILGEDVGAFGGCFGLTAGLFDEFGDKRVKDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 L+P+AE+M DF+T MD LVNQAAK+RYMFGGKIS+P+V+RLP G Sbjct: 61 GAIVGCAIGAAATGLKPIAEIMMGDFVTVAMDMLVNQAAKLRYMFGGKISLPMVVRLPGG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G+SA AQHS SLEAW+THVPG+KVVYPS DA G++LTAI+DDNPV +IE K +Y K Sbjct: 121 AGLSAAAQHSQSLEAWLTHVPGIKVVYPSTPADAAGLLLTAIDDDNPVAFIEHKAMYGLK 180 Query: 181 FEVDD----------TVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEA 230 EV D + +PFGVA +KREG+DV+II GK V +AL AA+ L+++GIE Sbjct: 181 GEVPDDIKPIPFGVADIKPIPFGVADIKREGNDVTIIATGKMVHEALKAAEQLSKDGIEV 240 Query: 231 EVIDLRSLYPLDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVR 290 EV+D R+L+PLDKE IF+SV KT + +VVTEE KRGGYGGE+SAMISE FD LDAPVVR Sbjct: 241 EVVDPRTLFPLDKETIFNSVNKTGKVVVVTEENKRGGYGGEISAMISEEIFDSLDAPVVR 300 Query: 291 IGALDTPVPYSPALEAYVLPNADDIIQGIKSMF 323 IGAL+TP+P++P LE+YV+P + DI+ +K +F Sbjct: 301 IGALNTPIPFAPNLESYVIPASKDIVNWVKGLF 333 >ref|NP_879468.1| putative pyruvate dehydrogenase E1 beta subunit [Bordetella pertussis Tohama I] emb|CAE44954.1| putative pyruvate dehydrogenase E1 beta subunit [Bordetella pertussis] Length = 324 Score = 336 bits (861), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 159/322 (49%), Positives = 217/322 (67%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M ++ + I + M EDP V L GED+ GG FGVT+GL + FG +R+RDTPISE Sbjct: 1 MAELRFAHAINRALAECMEEDPMVFLFGEDIAEAGGPFGVTRGLHDRFGSDRIRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 L+PV E+MF+DF+T MD LVNQAAK R+MFGG+ SVP+V+R P G Sbjct: 61 ATMANAAVGAALSGLKPVLEIMFMDFMTLTMDALVNQAAKARFMFGGQASVPMVVRTPHG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 GGISAG QHS LEAW H+PGLKVV PSN DA+G++ +AI D +PVV++E+K LY+ K Sbjct: 121 GGISAGPQHSQCLEAWFAHIPGLKVVCPSNPADAYGLLKSAIRDPDPVVFVENKALYAAK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV D V +P G A + R G D+++++YG V AA+ LA +GIEAEV+DLRS+ P Sbjct: 181 GEVPDDVGPIPLGQARIARAGRDLTVVSYGAMVHKVERAAEALARDGIEAEVLDLRSIQP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 D+ A+ +S+ +THR L+V E V+ G G E++A ++++ FDELDAP+VR+ A PVP+ Sbjct: 241 WDEAAVLASLRRTHRLLIVHEAVEAFGVGAEIAARMADIGFDELDAPIVRVAAPFVPVPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSM 322 +P+LEA P DII + + Sbjct: 301 APSLEAQYQPQEADIIAAARKL 322 >ref|NP_241643.1| acetoin dehydrogenase (TPP-dependent) beta chain [Bacillus halodurans] pir||A83747 acetoin dehydrogenase (TPP-dependent) beta chain BH0777 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04496.1| acetoin dehydrogenase (TPP-dependent) beta chain [Bacillus halodurans] Length = 327 Score = 335 bits (860), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 168/324 (51%), Positives = 221/324 (68%), Gaps = 1/324 (0%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 MR++TY E IRE M +M ++ +V +LGED+G YGG FGVT+G+ EEFG ERVR+TPISE Sbjct: 1 MREITYLEAIREAMTLEMRKNEDVFILGEDIGVYGGAFGVTRGMIEEFGSERVRNTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 +RP+ EL F DF+T MD +VNQAAK+RYM+GGK VP+VLR PAG Sbjct: 61 AAISGTAIGAALTGMRPILELQFSDFITIAMDNMVNQAAKLRYMYGGKAKVPMVLRTPAG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 G A AQHS SLEAW+TH+PGLKVV P+ A DA G++ AI+D+NPV++ E K+ Y K Sbjct: 121 SGTGAAAQHSQSLEAWMTHIPGLKVVQPATAYDAKGLLKAAIDDNNPVIFYEHKLCYRTK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 V + ++P G A VKR+G+DV+++ V AL AA L +EGI EVID R+L P Sbjct: 181 CHVPEEEYSIPLGKADVKRKGTDVTVVATAVMVHKALEAAVELEKEGISVEVIDPRTLVP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISEL-CFDELDAPVVRIGALDTPVP 299 LD+E I SV KT R +VV E VKRGG+GGE++++I+E FD LDAP+ R+G P+P Sbjct: 241 LDEETIIRSVKKTSRLIVVHEAVKRGGFGGEIASIIAESEAFDYLDAPIKRLGGKPVPIP 300 Query: 300 YSPALEAYVLPNADDIIQGIKSMF 323 Y+P LE +P DII+ +K Sbjct: 301 YNPTLERAAIPQVPDIIEAVKETL 324 >ref|NP_891237.1| putative pyruvate dehydrogenase E1 beta subunit [Bordetella bronchiseptica RB50] emb|CAE35067.1| putative pyruvate dehydrogenase E1 beta subunit [Bordetella bronchiseptica] Length = 324 Score = 335 bits (858), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 159/322 (49%), Positives = 216/322 (67%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 M ++ + I + M EDP V L GED+ GG FGVT+GL + FG +R+RDTPISE Sbjct: 1 MAELRFAHAINRALAECMEEDPMVFLFGEDIAEAGGPFGVTRGLHDRFGPDRIRDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 L+PV E+MF+DF+T MD LVNQAAK R+MFGG+ SVP+V+R P G Sbjct: 61 ATMANAAVGAALSGLKPVLEIMFMDFMTLTMDALVNQAAKARFMFGGQASVPMVVRTPHG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 GGISAG QHS LEAW H+PGLKVV PSN DA+G++ +AI D +PVV++E+K LY+ K Sbjct: 121 GGISAGPQHSQCLEAWFAHIPGLKVVCPSNPADAYGLLKSAIRDPDPVVFVENKALYAAK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV D +P G A V R G D+++++YG V AA+ LA +GIEAEV+DLRS+ P Sbjct: 181 GEVPDDAGPIPLGQARVARAGRDLTVVSYGAMVHKVERAAEALARDGIEAEVLDLRSIQP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 D+ A+ +S+ +THR L+V E V+ G G E++A ++++ FDELDAP+VR+ A PVP+ Sbjct: 241 WDEAAVLASLQRTHRLLIVHEAVEAFGVGAEIAARMADIGFDELDAPIVRVAAPFVPVPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSM 322 +P+LEA P DII + + Sbjct: 301 APSLEAQYQPQEADIIAAARKL 322 >ref|NP_342959.1| Pyruvate dehydrogenase, beta subunit (lipoamide). (pdhB-2) [Sulfolobus solfataricus] pir||F90311 hypothetical protein pdhB-2 [imported] - Sulfolobus solfataricus gb|AAK41749.1| Pyruvate dehydrogenase, beta subunit (lipoamide). (pdhB-2) [Sulfolobus solfataricus] Length = 324 Score = 324 bits (831), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 158/323 (48%), Positives = 217/323 (67%) Query: 1 MRKMTYGEGIREGMRSKMLEDPNVVLLGEDVGPYGGTFGVTKGLWEEFGEERVRDTPISE 60 MR++T+ E I E +R +M DP+V+L+GED+G YGG FGVTKGL E+FG +RV DTPISE Sbjct: 1 MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISE 60 Query: 61 XXXXXXXXXXXXXXLRPVAELMFIDFLTFGMDGLVNQAAKMRYMFGGKISVPLVLRLPAG 120 LRPV ELMF+DF MD + NQ AK+RYM GG++ VPL LR P G Sbjct: 61 AGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIG 120 Query: 121 GGISAGAQHSGSLEAWVTHVPGLKVVYPSNAQDAWGMMLTAIEDDNPVVYIESKVLYSKK 180 GISA AQHS +L + HVPGLKVV PS DA G+++++I DDNPVV++E KVLY K Sbjct: 121 AGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIRDDNPVVFLEHKVLYGIK 180 Query: 181 FEVDDTVAAVPFGVASVKREGSDVSIITYGKQVDDALNAAKVLAEEGIEAEVIDLRSLYP 240 EV + +P G A ++REG DV++I + V +L AA+ L++E I EVID+RS+ P Sbjct: 181 GEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP 240 Query: 241 LDKEAIFSSVAKTHRALVVTEEVKRGGYGGELSAMISELCFDELDAPVVRIGALDTPVPY 300 DKE + SV KT R ++V E+ R G+ +S++I++ F+ LDAP+ RI + P+P+ Sbjct: 241 FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPF 300 Query: 301 SPALEAYVLPNADDIIQGIKSMF 323 SP LE Y+LP++ I+ +KS+ Sbjct: 301 SPPLEQYILPDSKKIVNTVKSIL 323 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 464,258,261 Number of extensions: 18634810 Number of successful extensions: 49611 Number of sequences better than 1.0e-05: 1074 Number of HSP's gapped: 48707 Number of HSP's successfully gapped: 1077 Length of query: 323 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 197 Effective length of database: 415,135,104 Effective search space: 81781615488 Effective search space used: 81781615488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)