BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0032.p (156 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_228395.1| lipopolysaccharide biosynthesis protein BplA [T... 104 5e-22 gb|EAJ60280.1| unknown [environmental sequence] 102 4e-21 gb|EAC92601.1| unknown [environmental sequence] 100 9e-21 ref|ZP_00098828.1| COG0673: Predicted dehydrogenases and related... 99 4e-20 gb|EAB38883.1| unknown [environmental sequence] 95 5e-19 >ref|NP_228395.1| lipopolysaccharide biosynthesis protein BplA [Thermotoga maritima] pir||B72359 lipopolysaccharide biosynthesis protein BplA - Thermotoga maritima (strain MSB8) gb|AAD35670.1| lipopolysaccharide biosynthesis protein BplA [Thermotoga maritima] Length = 360 Score = 104 bits (260), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 52/144 (36%), Positives = 80/144 (55%), Gaps = 6/144 (4%) Query: 7 VAVADTDPVKAAEAAENYGC------EALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEF 60 VAV D +A AAE++ E +T+Y++LL R D+ ++ + T H + E Sbjct: 32 VAVCDLVEERANRAAEHFEKSGLRRPETMTNYRELLKREDVDVISIATESGKHYQITMEA 91 Query: 61 LEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGR 120 LEAG HVL EKP+AL + E++ +LG+ FQNR+N P +++ +DSG G+ Sbjct: 92 LEAGKHVLVEKPMALSTKHMNEMVELSKQKNLKLGVFFQNRFNPPVQEVRKKLDSGAFGK 151 Query: 121 LICMKGIVTWYRSEDYYTESGWGG 144 + V W R+EDYY ++ W G Sbjct: 152 IFYASVAVRWNRNEDYYKQASWRG 175 >gb|EAJ60280.1| unknown [environmental sequence] Length = 236 Score = 102 bits (253), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 53/144 (36%), Positives = 78/144 (54%) Query: 1 MEGARLVAVADTDPVKAAEAAENYGCEALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEF 60 M A LVA+ D +A E A+ +G E DY LL R DI V +CT HA Sbjct: 27 MADAELVAICSRDLDRAKEIADQFGGEPTADYDALLSRDDIDAVSICTASGEHATFGVPA 86 Query: 61 LEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGR 120 +AG HVL EKPI + + A + + A+ +LG++FQ R+ + +K+ ID GILG Sbjct: 87 AKAGKHVLVEKPIEIHLDKADELIDACDANGVKLGVIFQLRFLDACQEVKKAIDDGILGN 146 Query: 121 LICMKGIVTWYRSEDYYTESGWGG 144 + + +YR ++YY++S W G Sbjct: 147 PVMADCYMKFYRPQEYYSDSRWKG 170 >gb|EAC92601.1| unknown [environmental sequence] Length = 305 Score = 100 bits (250), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 54/142 (38%), Positives = 79/142 (55%), Gaps = 1/142 (0%) Query: 3 GARLVAVADTDPVKAAEAAENYGCEALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEFLE 62 G LVA D KA A+ YG A + + LL D+ IV +C+P+ LHA + + L+ Sbjct: 2 GCNLVATCDIISDKAEVLADKYGAIAYSSIEDLLLNNDVNIVSICSPNALHASHSIQSLK 61 Query: 63 AGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGRLI 122 +G HVL EKP+A++ + + A + +L ++ QNRYN P IK ID G LGR+ Sbjct: 62 SGKHVLCEKPMAINTYDCGEMIKEAEKANKRLFVIKQNRYNPPVAAIKSAIDEGKLGRIT 121 Query: 123 CMKGIVTWYRSEDYYTESGWGG 144 ++ W R+E+YY S W G Sbjct: 122 SVQLSCFWNRNENYYANS-WKG 142 >ref|ZP_00098828.1| COG0673: Predicted dehydrogenases and related proteins [Desulfitobacterium hafniense] Length = 377 Score = 98.6 bits (244), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 48/144 (33%), Positives = 80/144 (55%) Query: 1 MEGARLVAVADTDPVKAAEAAENYGCEALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEF 60 +E A LVAV D P +A A+ YG + T Y+++L + +I +V + T LHAP+ Sbjct: 58 LEEAELVAVCDIIPERAQAFADKYGAKPYTSYEEMLAKEEIDVVTIATESDLHAPIGITC 117 Query: 61 LEAGHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGR 120 +AG HV+ EKP+A+ + SA + +L ++ QNRYN +++ ++ G G+ Sbjct: 118 AKAGKHVMVEKPMAMTLASADELIRTCHEEGVKLSVIHQNRYNKSIKLMRQALEEGRFGK 177 Query: 121 LICMKGIVTWYRSEDYYTESGWGG 144 L + V W R++ YY ++ W G Sbjct: 178 LTHGQATVRWNRNDAYYAQAPWRG 201 >gb|EAB38883.1| unknown [environmental sequence] Length = 314 Score = 95.1 bits (235), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 50/141 (35%), Positives = 72/141 (51%) Query: 4 ARLVAVADTDPVKAAEAAENYGCEALTDYKQLLGRTDIGIVHLCTPHHLHAPMAAEFLEA 63 A LV V D D +A E E +G TD +++G V + TP HA Sbjct: 1 AELVGVTDIDSQRAEEIGERFGVPHFTDMDEMMGTVRPDAVSILTPSGDHADHTVRLAPY 60 Query: 64 GHHVLTEKPIALDVPSARRIQEVAAASQGQLGIVFQNRYNNPSIRIKETIDSGILGRLIC 123 G ++ EKP+AL + A R+ A+ +L +V QNR+N P +R++E IDSG GRL+ Sbjct: 61 GKVIIVEKPMALTLDDADRMIRACDANGCRLFVVKQNRFNLPVLRLREAIDSGRFGRLVS 120 Query: 124 MKGIVTWYRSEDYYTESGWGG 144 V W R ++YY + W G Sbjct: 121 GSVRVRWCRRQEYYDQDAWRG 141 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 222,871,987 Number of extensions: 8156273 Number of successful extensions: 19605 Number of sequences better than 1.0e-05: 573 Number of HSP's gapped: 19787 Number of HSP's successfully gapped: 573 Length of query: 156 Length of database: 757,237,578 Length adjustment: 114 Effective length of query: 42 Effective length of database: 447,716,292 Effective search space: 18804084264 Effective search space used: 18804084264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)