BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0548.0004.p (809 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||JC7657 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Clo... 115 3e-24 ref|ZP_00060158.1| COG4124: Beta-mannanase [Clostridium thermoce... 115 4e-24 gb|AAC44232.1| beta-1,4-mannanase 111 6e-23 gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] 103 1e-20 sp|P55296|MANA_PIRSP Mannan endo-1,4-beta-mannosidase A precurso... 103 1e-20 >pir||JC7657 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Clostridium thermocellum dbj|BAB19050.1| mannanase 26B [Clostridium thermocellum] Length = 591 Score = 115 bits (289), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 76/205 (37%), Positives = 106/205 (51%), Gaps = 12/205 (5%) Query: 538 VNPGATVEAAALLDRLYAATGNTIYTGQ------HNYYEEPAKWYDKAAELTGYYPALWG 591 VNP AT EA AL+ L G I +GQ HNY A+ + E TG PA+ G Sbjct: 180 VNPNATKEAKALMSYLVDIYGKHILSGQQEICGSHNYPGSEAE-FTYIQEKTGKLPAVRG 238 Query: 592 SDFAYYTGEDMGGLRQSMIDEAILQ--GKAGTMVTLTYHQTRPMDPATSGWESVTGDVTE 649 DF Y G G + E +++ + G + T+ +H P D S + T Sbjct: 239 FDFMNYRGN--GLMWDDQCAERVIEWYKEKGGIPTVCWHWFSPGDIGKKADNSFYTESTT 296 Query: 650 AQMTDIVTPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGAR-PEL 708 ++ +TPGTE +D +A L+Q+QD G+PVL+RP HE +FWWGA PE Sbjct: 297 FSISRALTPGTEENIALLNDIDTIARKLKQVQDAGVPVLFRPLHEAEGGWFWWGAEGPEP 356 Query: 709 FKQLWHNMYDRLTHLHGLNNLIWVW 733 +L+ +YD+ T+ +GLNNLIWVW Sbjct: 357 CVRLYRLLYDKFTNEYGLNNLIWVW 381 >ref|ZP_00060158.1| COG4124: Beta-mannanase [Clostridium thermocellum ATCC 27405] Length = 597 Score = 115 bits (288), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 76/205 (37%), Positives = 106/205 (51%), Gaps = 12/205 (5%) Query: 538 VNPGATVEAAALLDRLYAATGNTIYTGQ------HNYYEEPAKWYDKAAELTGYYPALWG 591 VNP AT EA AL+ L G I +GQ HNY A+ + E TG PA+ G Sbjct: 186 VNPNATKEAKALMSYLVDIYGKHILSGQQEICGSHNYPGSEAE-FTYIQEKTGKLPAIRG 244 Query: 592 SDFAYYTGEDMGGLRQSMIDEAILQ--GKAGTMVTLTYHQTRPMDPATSGWESVTGDVTE 649 DF Y G G + E +++ + G + T+ +H P D S + T Sbjct: 245 FDFMNYRGN--GLMWDDQCAERVIEWYKEKGGIPTVCWHWFSPGDIGKKADNSFYTESTT 302 Query: 650 AQMTDIVTPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGAR-PEL 708 ++ +TPGTE +D +A L+Q+QD G+PVL+RP HE +FWWGA PE Sbjct: 303 FSISRALTPGTEENIALLNDIDTMARKLKQVQDAGVPVLFRPLHEAEGGWFWWGAEGPEP 362 Query: 709 FKQLWHNMYDRLTHLHGLNNLIWVW 733 +L+ +YD+ T+ +GLNNLIWVW Sbjct: 363 CVRLYRLLYDKFTNEYGLNNLIWVW 387 >gb|AAC44232.1| beta-1,4-mannanase Length = 911 Score = 111 bits (278), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 74/207 (35%), Positives = 104/207 (50%), Gaps = 15/207 (7%) Query: 538 VNPGATVEAAALLDRLYAATGNTIYTGQHNYYEEPAKWYDKAAELTGYYPALWGSDFAYY 597 V P A L+ L + G I +GQ + K ++T YPA+ G DF Y Sbjct: 591 VTPNPHPNAQKLMKYLASIYGEKILSGQQS--SGDGKEIQMIFDVTKRYPAVRGFDFMDY 648 Query: 598 TGEDM-GGLRQSMIDEAILQGKAGTMVTLTYHQTRPM----DPATSGWESVTGDVTEAQM 652 + + G + + ++EAI K+G +V +H P P W G TEA Sbjct: 649 SPSRVEHGTKGTDVEEAIKWWKSGGIVAFCWHWNAPTGLIDQPGKEWWR---GFYTEATT 705 Query: 653 TDIV----TPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGAR-PE 707 DI P + Y +D +A L++LQ EG+PVL+RP HE + +FWWGA+ PE Sbjct: 706 FDIKKAMDNPNSTEYKLILRDIDAIAEQLKRLQKEGVPVLFRPLHEASGGWFWWGAKGPE 765 Query: 708 LFKQLWHNMYDRLTHLHGLNNLIWVWN 734 + +LW M+DRL + H LNNLIWVWN Sbjct: 766 PYIKLWKLMFDRLVNYHKLNNLIWVWN 792 >gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] Length = 398 Score = 103 bits (258), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 69/203 (33%), Positives = 99/203 (48%), Gaps = 12/203 (5%) Query: 538 VNPGATVEAAALLDRLYAATGNTIYTGQHNYYEEPAKWYDKAAELTGYYPALWGSDFAYY 597 V P + EA L+D L + G +GQ Y + W +T +PA+ G D Y Sbjct: 85 VTPNPSKEAQKLMDYLVSIYGKYTLSGQMGYKDAFWIW-----NITDKFPAICGFDMMDY 139 Query: 598 TGEDM-GGLRQSMIDEAILQGKAGTMVTLTYHQTRPMDP-ATSGWESVTGDVTEAQMTDI 655 + + G +++AI G +V +H P T G E G T A DI Sbjct: 140 SPSRVERGASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLYDTPGKEWWRGFYTNATSFDI 199 Query: 656 V----TPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLWRPYHEMNAEFFWWGAR-PELFK 710 P +E Y +D +A L++LQ+ +P+LWRP HE +FWWGA+ PE K Sbjct: 200 EYALNHPESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWGAKGPEACK 259 Query: 711 QLWHNMYDRLTHLHGLNNLIWVW 733 +LW ++DRL + H +NNLIWVW Sbjct: 260 KLWRLLFDRLVNYHKINNLIWVW 282 >sp|P55296|MANA_PIRSP Mannan endo-1,4-beta-mannosidase A precursor (Beta-mannanase A) (1,4-beta-D-mannan mannanohydrolase A) emb|CAA62968.1| mannan endo-1,4-beta-mannosidase; mannanase A [Piromyces sp.] Length = 606 Score = 103 bits (258), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 70/227 (30%), Positives = 112/227 (49%), Gaps = 22/227 (9%) Query: 524 AASALQVTTGKMAP--VNPGATVEAAALLDRLYAATGNTIYTGQHNYYEEPAKWYDKAAE 581 A +A + + K+ P VNP A A L D L G I +GQ + D+ E Sbjct: 142 APNAAKDVSSKLNPTLVNPKAIPAAKKLYDFLKTNYGKRILSGQVGAAGQAG---DEGQE 198 Query: 582 L------TGYYPALWGSDFAYYTGEDMGGLRQSMIDEAIL------QGKAGTMVTLTYHQ 629 + TG PA+W DF + + + + I E + +GK ++ +H Sbjct: 199 IQRIQKATGKLPAVWNMDFIFESNDCTWRPQNPDITEMAINWWKKYEGKG--IMAAQWHW 256 Query: 630 TRPMDPATSGWESVTGDVTEAQMTDIVTPGTELYNQWAAQVDEVAGYLEQLQDEGIPVLW 689 + T + + D T + + VT GT Y + +D V+G++++LQ +P++W Sbjct: 257 N--IAGKTGDFAFYSKDTT-FNLENAVTEGTWEYEKIIKDIDRVSGHIKKLQAVNMPLIW 313 Query: 690 RPYHEMNAEFFWWGARPELFKQLWHNMYDRLTHLHGLNNLIWVWNPN 736 RP HE N ++FWWG P+ +LW +Y+R+ + HGLNNLIW+WN N Sbjct: 314 RPLHENNGDWFWWGNNPKACAKLWKILYERMVNYHGLNNLIWLWNGN 360 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,281,510,345 Number of extensions: 55666706 Number of successful extensions: 128590 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 128602 Number of HSP's successfully gapped: 46 Length of query: 809 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 675 Effective length of database: 393,414,312 Effective search space: 265554660600 Effective search space used: 265554660600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)