BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0031.p (588 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243939.1| PTS system, enzyme I [Bacillus halodurans] >gi|... 511 e-143 ref|NP_349687.1| Phosphoenolpyruvate-protein kinase (PTS system ... 506 e-142 ref|NP_623871.1| Phosphoenolpyruvate-protein kinase (PTS system ... 503 e-141 ref|NP_563273.1| phosphoenolpyruvate-protein phosphotransferase ... 498 e-139 sp|P42014|PT1_BACST Phosphoenolpyruvate-protein phosphotransfera... 479 e-134 >ref|NP_243939.1| PTS system, enzyme I [Bacillus halodurans] sp|Q9K8D3|PT1_BACHD Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) pir||A84034 PTS system, enzyme I ptsI [imported] - Bacillus halodurans (strain C-125) dbj|BAB06792.1| PTS system, enzyme I [Bacillus halodurans] Length = 572 Score = 511 bits (1315), Expect = e-143, Method: Compositional matrix adjust. Identities = 247/552 (44%), Positives = 378/552 (68%), Gaps = 3/552 (0%) Query: 15 KAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFD 74 KAF+ + E ++ + + D++ E E+L + SK+E+ IK + +G +++ IF Sbjct: 18 KAFI--HLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKDQTEASMGADKAEIFA 75 Query: 75 AHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVG 134 AHL +LDDP + I+G IE + AE A+KE D FV+MF+ +D+EYMKERA DI+DV Sbjct: 76 AHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDNEYMKERAADIRDVS 135 Query: 135 NRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMA 194 R+L HL+G +L + + +++A++L+PS A LN +V G T +GG+TSHS+IM+ Sbjct: 136 KRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMS 195 Query: 195 RALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQL 254 R+L IP V G + ++ IQ GD++++DG G++ + P E +K Y +RA +++K++ Sbjct: 196 RSLEIPAVVGTK-EVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEW 254 Query: 255 ELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQ 314 L + TKDG + LA NI + K++D L G +G+GL+RTEFLYMDR PTEEEQ Sbjct: 255 AKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFLYMDRDQLPTEEEQ 314 Query: 315 FEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQ 374 FE YK V +++ + VVIRTLDIGGDK L Y QLP E NPFLG+RAIR+ L+++D+F+TQ Sbjct: 315 FEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAIRLCLEKQDIFRTQ 374 Query: 375 MTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVP 434 + A+LRAS YGN+K+MFPMI+++EE++ AKA++ E K++L +GV + +I VGIM+E+P Sbjct: 375 LRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDVSDSIEVGIMVEIP 434 Query: 435 AAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAAR 494 + + A+L A+EVDFFSIGTNDL+QY +A DRMNE+++++Y PYHP +LR++ M ++AA Sbjct: 435 STAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPAILRLVDMVIKAAH 494 Query: 495 RVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALNTLASEARETAKSCF 554 G + +CGEMA DE ++PL L +G+ + SMS ++L + + L E + A+ Sbjct: 495 SEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKLSKEELKPFAQKAL 554 Query: 555 RHRTSTETEEIL 566 T+ E E+++ Sbjct: 555 MLDTAEEVEQLV 566 >ref|NP_349687.1| Phosphoenolpyruvate-protein kinase (PTS system enzyme I) [Clostridium acetobutylicum] pir||H97279 phosphoenolpyruvate-protein kinase (PTS system enzyme I) [imported] - Clostridium acetobutylicum gb|AAK81027.1| Phosphoenolpyruvate-protein kinase (PTS system enzyme I) [Clostridium acetobutylicum] Length = 539 Score = 506 bits (1304), Expect = e-142, Method: Compositional matrix adjust. Identities = 242/520 (46%), Positives = 364/520 (70%), Gaps = 3/520 (0%) Query: 17 FVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFDAH 76 F+ + E + + +++ D+ E RL + +++++ IK + + +G +++++F++H Sbjct: 18 FIKADNEVKIVEKKIS--DIESEKARLQGAVEKAREQLTKIKEKAEKDLGADKAAVFESH 75 Query: 77 LAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVGNR 136 + LDDP F+ II + AE A++ + ++VA+F+ ++DEY+KER D+KDVGNR Sbjct: 76 MMFLDDPDFIGSAENIISDEMINAEKALENVMKNYVAIFEGIEDEYVKERIADVKDVGNR 135 Query: 137 LLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMARA 196 +L++L G +L ++VA +L+PS A L+ V+G +T +GG+TSHS+IMAR Sbjct: 136 VLQNLAGNDMTSLADVDNNTVVVAHDLTPSDTAQLDKNRVIGFLTNIGGRTSHSAIMART 195 Query: 197 LGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQLEL 256 L IP V GL++ I+ ++ GD +V+DG G+ + PDE TLKEY +++A+ ++K+E+L Sbjct: 196 LEIPAVVGLQD-IVESVKNGDTIVVDGAEGIAIINPDEATLKEYEAKKAEYEKKQEKLRE 254 Query: 257 LATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQFE 316 L V+ TK+G ++ + GNI K+++ L+ G GVGLFRTEFLYMDR PTE+EQFE Sbjct: 255 LINVETRTKEGKRVEVCGNIGKAKDVEAVLQNGGDGVGLFRTEFLYMDRDQMPTEDEQFE 314 Query: 317 VYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQMT 376 YK V EK+G + VVIRTLDIGGDK L Y LP+E NPFLGYRAIR+ L RKD+FK Q+ Sbjct: 315 AYKAVVEKMGEKPVVIRTLDIGGDKKLPYLPLPEEMNPFLGYRAIRLCLGRKDIFKIQLR 374 Query: 377 AILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVPAA 436 A+LRAS YGN+K+MFPMISS+EE +AK VL E ELD++G YN + GIM+E+PAA Sbjct: 375 ALLRASVYGNLKIMFPMISSLEEFLSAKEVLKECMGELDKEGKKYNAKLETGIMVEIPAA 434 Query: 437 VMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAARRV 496 + ++ LA+ VDFFSIGTNDL+QY LA DRMNE+++++Y+P HP VL++I+MT+++A + Sbjct: 435 AVNSEELAKYVDFFSIGTNDLIQYTLAADRMNEKVSYLYNPMHPAVLKLIKMTIQSAHKE 494 Query: 497 GIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKH 536 G +CGEMA DE+++P +E G+ + SMS ++L K Sbjct: 495 GKWCGMCGEMAGDEKAIPTLVEYGLDEFSMSASSILTAKE 534 >ref|NP_623871.1| Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria) [Thermoanaerobacter tengcongensis] gb|AAM25475.1| Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria) [Thermoanaerobacter tengcongensis] Length = 573 Score = 503 bits (1295), Expect = e-141, Method: Compositional matrix adjust. Identities = 250/552 (45%), Positives = 379/552 (68%), Gaps = 1/552 (0%) Query: 15 KAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFD 74 KAF+ + ++ ++ + +E + + + +++EIE IK + + G E++ IF+ Sbjct: 15 KAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEKALKEFGKEKAEIFE 74 Query: 75 AHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVG 134 AHL + DP + + +I+ + A+ AV + I+ ++ + L+DEY+KERAVD++DVG Sbjct: 75 AHLMLASDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVG 134 Query: 135 NRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMA 194 NR++++LLG V L + +++A++L+PS A + VLG T +GG+TSH++IMA Sbjct: 135 NRIIENLLGVKSVNLSDLEEEVVVIARDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMA 194 Query: 195 RALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQL 254 R+L IP V GL N + + ++ GDL+++DG G++ + PDE T+++Y S++ ++K E L Sbjct: 195 RSLEIPAVVGLGN-VTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYKSKKESYEKKVEGL 253 Query: 255 ELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQ 314 + L + A T DG ++ LA NI + K++ AL GA+GVGLFRTEFLYMDR+S P+EEEQ Sbjct: 254 KQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFLYMDRNSLPSEEEQ 313 Query: 315 FEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQ 374 FE YK V EK+G V IRTLDIGGDK L Y +P E NPFLGYRAIR+ LDR D+FKTQ Sbjct: 314 FEAYKEVVEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRPDIFKTQ 373 Query: 375 MTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVP 434 + AILRAS YGN+++M+PMISSVEEV+ A ++L EVK ELD++GV Y++ I VGIM+E+P Sbjct: 374 LRAILRASAYGNVQIMYPMISSVEEVRKANSILEEVKAELDREGVKYDKEIKVGIMVEIP 433 Query: 435 AAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAAR 494 +A + AD+LA+EVDFFSIGTNDL QY LAVDRMNE + Y P+HP +LR+++M ++AA Sbjct: 434 SAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPAILRLVKMVIDAAH 493 Query: 495 RVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALNTLASEARETAKSCF 554 + G ++CGEMA D + + L +G+ + SMS ++ ++K+ N +A+E A+ Sbjct: 494 KEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNVEYEKAKEIAEKAL 553 Query: 555 RHRTSTETEEIL 566 + E E+++ Sbjct: 554 NMSEAREIEKMM 565 >ref|NP_563273.1| phosphoenolpyruvate-protein phosphotransferase [Clostridium perfringens] dbj|BAB82063.1| phosphoenolpyruvate-protein phosphotransferase [Clostridium perfringens str. 13] Length = 539 Score = 498 bits (1281), Expect = e-139, Method: Compositional matrix adjust. Identities = 249/519 (47%), Positives = 351/519 (67%), Gaps = 3/519 (0%) Query: 17 FVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFDAH 76 F+ + E + D +V+ D+A E E+L + + SK+++E IK + +G E+ +F+AH Sbjct: 17 FIQEHEEIIISDAKVS--DIAAEKEKLSKALAQSKEQLEAIKEKTANEIGEHEAQVFEAH 74 Query: 77 LAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVGNR 136 L +LDD F ++ IE A AV+ D FV +FD +DD YM+ERA DIKDV R Sbjct: 75 LMLLDDVEFTGQMEMTIENDQLNAMKAVQNVTDTFVMIFDSMDDPYMRERAADIKDVSKR 134 Query: 137 LLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMARA 196 ++ +L G + + ++VA +L+PS A L+ + V+G +T +GG+TSHS+IMAR Sbjct: 135 IIANLAGKGGNGMENVGANTVVVAHDLTPSDTAQLDRSKVIGFLTNIGGRTSHSAIMART 194 Query: 197 LGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQLEL 256 L IP V GL + I T ++ GD +++DG GV + PDE T+ EY +R K + ++E+L+ Sbjct: 195 LEIPAVVGLGD-ITTSVKNGDTVIVDGIEGVAIINPDEATINEYKARLEKFKAEQEELKK 253 Query: 257 LATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQFE 316 L V TK G ++ + GNI +++D L G GVGLFRTEFLYMDR PTE+EQFE Sbjct: 254 LIDVKTTTKSGRRIEVCGNIGKPEDIDQVLANGGDGVGLFRTEFLYMDRDEAPTEDEQFE 313 Query: 317 VYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQMT 376 YK V EK + VVIRTLDIGGDK L Y LP+E NPFLGYRAIR+ LDRKD+F+ Q+ Sbjct: 314 AYKYVLEKADGKQVVIRTLDIGGDKTLPYLPLPEEMNPFLGYRAIRLCLDRKDIFRVQIR 373 Query: 377 AILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVPAA 436 A+LRAS YGN+ +MFPMIS +EE Q AKA + E K EL +G+ Y+ +I GIM+E+PAA Sbjct: 374 ALLRASVYGNLAVMFPMISGLEEFQQAKAFVEECKAELKAEGIAYSDSIQWGIMVEIPAA 433 Query: 437 VMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAARRV 496 + AD LA+ VDFFSIGTNDL+QY LA DRM+E+++++Y+P HP VLR+I+MT++ A + Sbjct: 434 AVYADELAKHVDFFSIGTNDLIQYTLAADRMSEKVSYLYNPMHPAVLRLIKMTIDGAHKH 493 Query: 497 GIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVK 535 G + +CGEMA DER++P +E G+ + SMS ++L K Sbjct: 494 GKWVGMCGEMAGDERAIPTLVEYGLDEFSMSATSILTAK 532 >sp|P42014|PT1_BACST Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase system, enzyme I) gb|AAA86049.1| PTS enzyme I Length = 578 Score = 479 bits (1234), Expect = e-134, Method: Compositional matrix adjust. Identities = 244/540 (45%), Positives = 361/540 (66%), Gaps = 7/540 (1%) Query: 35 DLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFDAHLAILDDPVFMSEIRGIIE 94 D+ E RL + +K+E+E IK+ E +G ++++IF AHL +LDDP ++ I+ I+ Sbjct: 41 DVEAEIARLEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLLVLDDPELLNPIKEKIQ 100 Query: 95 RQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVGNRLLKHLLGAPEVTLPS-DT 153 + AE A+ E F++MF+ +D+EYMKERA DI+DV R+L HLLG ++ PS + Sbjct: 101 TERVNAEYALDETALFFISMFEAMDNEYMKERAADIRDVTKRVLAHLLGVT-ISNPSLIS 159 Query: 154 QPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMARALGIPLVAGLENKILTPI 213 + +++A++L+PS A LN YV G T +GG+TSHS+IMAR+L IP V G + + + Sbjct: 160 EEVVIIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKT-VTAEV 218 Query: 214 QTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQLELLATVDAVTKDGVQLRLA 273 + GD++++DG G + + P L +Y +RA+ + +K + L VT DG+ + LA Sbjct: 219 KNGDIVIVDGLDGQVIINPSPELLAQYEQKRARYEAQKAEWAKLVHEATVTADGIHVELA 278 Query: 274 GNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQFEVYKLVAEKVGNETVVIR 333 NI + ++ AL GA+G+GL+RTEFLYM RS PTE+EQF YK V E++ + VV+R Sbjct: 279 ANIGTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFVAYKTVLEQMNGKPVVVR 338 Query: 334 TLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQMTAILRASHYGNIKMMFPM 393 TLDIGGDK L Y QLP E NPFLG+RAIR+ L+ +DMF+TQ+ A+LRAS YGN+K+MFPM Sbjct: 339 TLDIGGDKELPYLQLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALLRASVYGNLKIMFPM 398 Query: 394 ISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVPAAVMIADLLAEEVDFFSIG 453 I++++E + AKA+L E KE L +QGV I VG+M+E+PAA ++AD A+EVDFFSIG Sbjct: 399 IATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVMADQFAKEVDFFSIG 458 Query: 454 TNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAARRVGIDISVCGEMAADERSL 513 TNDL+QY +A DRMNE++A++Y PY+P +LR+I ++AA R G + +CGEMA D ++ Sbjct: 459 TNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREGKWVGMCGEMAGDPIAI 518 Query: 514 PLWLEMGISDLSMSPQALLKVKHRALNTLASEARETAKSCFRHRTSTETEEILTAFAGRS 573 P+ L +G+ + SMS ++L + + L A+E A S T E + +F R+ Sbjct: 519 PILLALGLDEFSMSATSILPARAQ----LKKLAKEEAARIKETVLSLGTAEEVVSFVKRT 574 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 793,612,264 Number of extensions: 31721571 Number of successful extensions: 100589 Number of sequences better than 1.0e-05: 921 Number of HSP's gapped: 99039 Number of HSP's successfully gapped: 1003 Length of query: 588 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 457 Effective length of database: 401,559,609 Effective search space: 183512741313 Effective search space used: 183512741313 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)