BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0023.p (210 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_390847.1| acetoin dehydrogenase [Bacillus subtilis] >gi|7... 252 3e-66 ref|NP_981095.1| acetoin utilization protein AcuA [Bacillus cere... 238 5e-62 ref|NP_834366.1| Acetoin utilization protein acuA [Bacillus cere... 238 6e-62 ref|NP_658695.1| Acetyltransf, Acetyltransferase (GNAT) family [... 236 2e-61 ref|NP_244101.1| acetoin dehydrogenase [Bacillus halodurans] >gi... 234 9e-61 >ref|NP_390847.1| acetoin dehydrogenase [Bacillus subtilis] sp|P39065|ACUA_BACSU Acetoin utilization protein acuA pir||S39645 acetoin utilization protein acuA - Bacillus subtilis gb|AAA68286.1| acetoin utilization protein gb|AAC00396.1| acetoin catabolism protein AcuA [Bacillus subtilis] emb|CAB14947.1| acetoin dehydrogenase [Bacillus subtilis subsp. subtilis str. 168] Length = 210 Score = 252 bits (644), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 120/210 (57%), Positives = 148/210 (70%) Query: 1 MEHRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPE 60 MEH K S + + + GP+SP+ L G + H DL AFR P+EQ EAL++IAGLPE Sbjct: 1 MEHHKTYHSANIKTATGSLLIEGPVSPEDLAGYEFHKDLTAFRPPREQHEALVDIAGLPE 60 Query: 61 GRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSA 120 GR+I A + I GYVT+ YPD LE WS+G MEDLIELGA+EVA +RG + K L+T + Sbjct: 61 GRIIIARDGRTIVGYVTYLYPDPLERWSEGNMEDLIELGAIEVAPDYRGCAVGKTLLTVS 120 Query: 121 FEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPA 180 EQ+EN IV TTEYYWHWDLKG DVW YR++MEK+M +VW+ATD+PEI SHPA Sbjct: 121 MMDEQMENYIVMTTEYYWHWDLKGMKKDVWEYRKIMEKMMNAGGLVWFATDEPEISSHPA 180 Query: 181 NCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 NCLM RIG+ V S E FDR+RF R+MY Sbjct: 181 NCLMARIGKNVSQESIEQFDRLRFYHRYMY 210 >ref|NP_981095.1| acetoin utilization protein AcuA [Bacillus cereus ATCC 10987] gb|AAS43703.1| acetoin utilization protein AcuA [Bacillus cereus ATCC 10987] Length = 214 Score = 238 bits (608), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 110/208 (52%), Positives = 145/208 (69%) Query: 3 HRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPEGR 62 H+KI + L + + + GP+S L+ + HPDL AFR ++Q +A++EI+ LPE R Sbjct: 7 HKKIYNARNLKTAKGTLIIEGPVSTHNLEMYEFHPDLIAFRPAEQQYKAIVEISKLPEAR 66 Query: 63 VIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSAFE 122 +I A DQ I GYVT+ YPD LE WS+G ME+LIELGA+EV FRG + K L+ + Sbjct: 67 LIIARHDQTIVGYVTYLYPDPLERWSEGKMENLIELGAIEVVPAFRGCAVGKNLLEVSMM 126 Query: 123 REQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPANC 182 + +E+ I+ TTEYYWHWDLK +GL+VW YR++MEK+M + W ATDDPEICSHPANC Sbjct: 127 DDYMEDYIILTTEYYWHWDLKQTGLNVWEYRKVMEKMMNAGGLQWMATDDPEICSHPANC 186 Query: 183 LMVRIGREVPMSSQETFDRVRFRQRFMY 210 LMVRIG+ V S + FDR+RF RFMY Sbjct: 187 LMVRIGKRVDTDSIQAFDRLRFHNRFMY 214 >ref|NP_834366.1| Acetoin utilization protein acuA [Bacillus cereus ATCC 14579] gb|AAP11567.1| Acetoin utilization protein acuA [Bacillus cereus ATCC 14579] Length = 214 Score = 238 bits (607), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 110/208 (52%), Positives = 145/208 (69%) Query: 3 HRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPEGR 62 H+KI + L + + + GP+S L+ + HPDL AFR ++Q +A++EI+ LPE R Sbjct: 7 HKKIYNARNLKTAKGTLIIEGPVSTHNLEMYEFHPDLIAFRPAEQQYKAIVEISKLPEAR 66 Query: 63 VIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSAFE 122 +I A DQ I GYVT+ YPD LE WS+G ME+LIELGA+EV FRG + K L+ + Sbjct: 67 LIIARHDQTIVGYVTYLYPDPLERWSEGKMENLIELGAIEVVPAFRGSSVGKNLLEVSMM 126 Query: 123 REQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPANC 182 + +E+ I+ TTEYYWHWDLK +GL+VW YR++MEK+M + W ATDDPEICSHPANC Sbjct: 127 DDYMEDYIILTTEYYWHWDLKQTGLNVWEYRKVMEKMMNAGGLQWMATDDPEICSHPANC 186 Query: 183 LMVRIGREVPMSSQETFDRVRFRQRFMY 210 LMVRIG+ V S + FDR+RF RFMY Sbjct: 187 LMVRIGKRVDTDSIQAFDRLRFHNRFMY 214 >ref|NP_658695.1| Acetyltransf, Acetyltransferase (GNAT) family [Bacillus anthracis A2012] ref|NP_847114.1| acetoin utilization protein AcuA [Bacillus anthracis str. Ames] gb|AAP28600.1| acetoin utilization protein AcuA [Bacillus anthracis str. Ames] Length = 210 Score = 236 bits (603), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 110/210 (52%), Positives = 145/210 (69%) Query: 1 MEHRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPE 60 M H+KI + L + + + GP+S L+ + H DL AFR ++Q +A++EI+ LPE Sbjct: 1 MIHKKIYNARNLKTAKGTLIIEGPVSTHNLEMYEFHTDLVAFRPAEQQYKAIVEISKLPE 60 Query: 61 GRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSA 120 R+I A DQ I GYVT+ YPD LE WS+G ME+LIELGA+EV FRG + K L+ + Sbjct: 61 ARLIIARHDQTIVGYVTYLYPDPLERWSEGKMENLIELGAIEVVPAFRGCAVGKNLLEVS 120 Query: 121 FEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPA 180 + +E+ I+ TTEYYWHWDLK +GL+VW YR++MEK+M + W ATDDPEICSHPA Sbjct: 121 MMDDYMEDYIILTTEYYWHWDLKQTGLNVWEYRKVMEKMMNAGGLQWMATDDPEICSHPA 180 Query: 181 NCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 NCLMVRIG+ V S + FDR+RF RFMY Sbjct: 181 NCLMVRIGKRVDTDSIQAFDRLRFHNRFMY 210 >ref|NP_244101.1| acetoin dehydrogenase [Bacillus halodurans] pir||C84054 acetoin dehydrogenase acuA [imported] - Bacillus halodurans (strain C-125) dbj|BAB06954.1| acetoin dehydrogenase [Bacillus halodurans] Length = 210 Score = 234 bits (597), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 111/192 (57%), Positives = 140/192 (72%) Query: 19 ITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPEGRVIAAVVDQVIAGYVTF 78 + + GPLS L L H L AFR P+EQ +ALIEIA LPEGRV +Q+I GYVTF Sbjct: 19 LVLEGPLSGKQLSELDFHEGLVAFRPPKEQRKALIEIADLPEGRVNIVRHEQMIVGYVTF 78 Query: 79 HYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSAFEREQLENCIVFTTEYYW 138 YPD LE WSQG +E+LIELGA+E+A +RG + K L+ + + + +E+ I+ TTEYYW Sbjct: 79 VYPDPLERWSQGQIENLIELGAIEIAPEYRGAQVGKNLLRLSMKDDAMEDYIIITTEYYW 138 Query: 139 HWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPANCLMVRIGREVPMSSQET 198 HWDLKGSGL+VW YR++MEK+M + WYATDDPEICSHPANCLM RIG+ V S + Sbjct: 139 HWDLKGSGLNVWEYRKVMEKMMNAGGLEWYATDDPEICSHPANCLMARIGKRVAPESIQA 198 Query: 199 FDRVRFRQRFMY 210 FD++RF+ RFMY Sbjct: 199 FDQLRFQNRFMY 210 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 323,021,303 Number of extensions: 12954827 Number of successful extensions: 31338 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 31337 Number of HSP's successfully gapped: 11 Length of query: 210 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 90 Effective length of database: 431,425,698 Effective search space: 38828312820 Effective search space used: 38828312820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 123 (52.0 bits)