BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0004.p (575 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_712310.1| DNA repair helicase [Leptospira interrogans ser... 516 e-145 ref|NP_825089.1| putative ATP-dependent DNA helicase [Streptomyc... 478 e-133 ref|NP_737501.1| putative DNA helicase [Corynebacterium efficien... 476 e-133 ref|NP_600045.1| putative helicase [Corynebacterium glutamicum A... 474 e-132 ref|NP_488743.1| DNA repair helicase [Nostoc sp. PCC 7120] >gi|2... 473 e-132 >ref|NP_712310.1| DNA repair helicase [Leptospira interrogans serovar lai str. 56601] gb|AAN49328.1| DNA repair helicase [Leptospira interrogans serovar lai str. 56601] gb|AAS70380.1| ATP-dependent DNA helicase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 569 Score = 516 bits (1328), Expect = e-145, Method: Compositional matrix adjust. Identities = 265/555 (47%), Positives = 351/555 (63%), Gaps = 10/555 (1%) Query: 15 IIRKDRTILLECGHPGFEAARTALADFAELVKSPPAYHTYRITQLSLWNAAAAGKTAQEI 74 I++ DRT+LLE +P FEA ++ ++ FAEL KSP HTYRI+ LSLWNAA+ +A EI Sbjct: 6 IVQSDRTMLLEVDNPEFEACQSVVSKFAELEKSPEYLHTYRISPLSLWNAASIKMSADEI 65 Query: 75 LAVLSLLSRCGIPAGLEEEVRQLVSRYGRLELHSGADGGSLMVLRADNPVXXXXXXXXXX 134 + L SR +P + E+R+ +SRYG+++L + G L +L N Sbjct: 66 VECLEKFSRYSVPKNIVNEIREQISRYGKVKL-VKEESGELAIL--SNEKGFLQEIGNHR 122 Query: 135 XXXXXXNRTAPLECRCPAEQRGLLKQELARLGFPVLDYAGYRDGQALNVPWKKAAGSGAV 194 T P + E RG +KQ L ++GFPV D AGY +G + + SG Sbjct: 123 AVQPFIESTFPDKIYIKKEYRGHIKQALIKIGFPVEDLAGYDEGNKYGFNLRPTSISGKK 182 Query: 195 YGESVGLRDYQEEAVRRFXXXXXXXXXXXXX-LPCGAGKTLVGLAVLESLQCETLILTSN 253 +G +RDYQ V F LPCGAGKT+VG+ V++ + ETLIL +N Sbjct: 183 FG----MRDYQRACVEVFHAGGGNEGGSGVVVLPCGAGKTIVGIGVMQIVGAETLILVTN 238 Query: 254 ATSVAQWKAELLKRTGLDEEAVGEYSGVKREVRPITVATYQILTHRRSKGGPFGHMNLFN 313 S+ QW+ E+L +T + E +GEYSG +E+RPIT+ATY ILTHR+ KGG F H +LF Sbjct: 239 TLSIRQWRNEILDKTDIPPEDIGEYSGEVKEIRPITIATYNILTHRKKKGGDFTHFHLFG 298 Query: 314 ERNWGLIIYDEVHLLPAPVFRATADIQATRRLGLXXXATLVREDGREDDVFSLIGPKCYE 373 NWGLI+YDEVHLLPAPVFR T+++QA RRLGL ATLVREDG E+DVFSLIGPK Y+ Sbjct: 299 ANNWGLIVYDEVHLLPAPVFRMTSELQAKRRLGL--TATLVREDGLEEDVFSLIGPKKYD 356 Query: 374 LPWKVLEKQGWIAAVDCIEVVVPMGTDIRHRYMYAGAKEQFRVXXXXXXXXXXXXXXXXX 433 +PWK LE + WIA C E+ V M D+R +Y A +E+FR+ Sbjct: 357 VPWKELESKSWIAEAKCKEIRVNMEDDLRLKYSIADDREKFRLASENPEKMKAIGLIMKK 416 Query: 434 XXXXXVLVIGQYLDQLKQLAVSLGAPLITGKTSQQERNELYAAFNEGAIRLLVVSKVANF 493 +LVIGQY++QL++++ PLITGKT ER LY AF G I+ LVVSKVANF Sbjct: 417 HSESHLLVIGQYINQLEEISKKFNIPLITGKTPLPERQTLYDAFRSGKIKSLVVSKVANF 476 Query: 494 AVNLPDASVAIEVSGAYGSRQEEAQRLGRILRPKPGENKAYFYTLVSGDSREQDFALRRR 553 +++LPDA++AI+VSG +GSRQEEAQRLGRILRPK +N A FY+L+S D+ E+ F R+ Sbjct: 477 SIDLPDANIAIQVSGTFGSRQEEAQRLGRILRPKGHDNTAVFYSLISRDTNEERFGQNRQ 536 Query: 554 LFLTEQGYDYAVQTV 568 LFLTEQGY+Y + T+ Sbjct: 537 LFLTEQGYEYEIYTL 551 >ref|NP_825089.1| putative ATP-dependent DNA helicase [Streptomyces avermitilis MA-4680] dbj|BAC71624.1| putative ATP-dependent DNA helicase [Streptomyces avermitilis MA-4680] Length = 547 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 258/555 (46%), Positives = 341/555 (61%), Gaps = 19/555 (3%) Query: 11 KGTCIIRKDRTILLECGHPGFEAARTALADFAELVKSPPAYHTYRITQLSLWNAAAAGKT 70 G I++ D+T+LLE H R A+A FAEL ++P HTYR+T L LWNA AAG Sbjct: 2 NGPLIVQSDKTLLLEVDHEQANECRRAIAPFAELERAPEHIHTYRVTPLGLWNARAAGHD 61 Query: 71 AQEILAVLSLLSRCGIPAGLEEEVRQLVSRYGRLELHSGADGGSLMVLRADNPVXXXXXX 130 A++++ L SR +P L ++ + + RYGRL L G L++ D PV Sbjct: 62 AEQVVDALVQYSRYPVPHALLVDIAETMDRYGRLTLSKHPAHG-LVLTTTDRPVLEEILR 120 Query: 131 XXXXXXXXXXNRTAPLECRCPAEQRGLLKQELARLGFPVLDYAGYRDGQALNVPWKKAAG 190 R P +RG +KQ L +LG+P D AGY DG+A + + Sbjct: 121 SKRVAPLVGA-RLDPDTVAVHPSERGQIKQTLLKLGWPAEDLAGYVDGEAHPIELAE--- 176 Query: 191 SGAVYGESVGLRDYQEEAVRRFXXXXXXXXXXXXXLPCGAGKTLVGLAVLESLQCETLIL 250 + LR YQ++AV F LPCGAGKTLVG + + TLIL Sbjct: 177 ------DGWALRPYQKQAVENFWHGGSGVVV----LPCGAGKTLVGAGSMAQAKATTLIL 226 Query: 251 TSNATSVAQWKAELLKRTGLDEEAVGEYSGVKREVRPITVATYQILTHRRSKGGPFGHMN 310 +N S QWK EL+KRT L E+ +GEYSG K+E+RP+T+ATYQ+LT RR G + H+ Sbjct: 227 VTNTVSARQWKHELVKRTSLTEDEIGEYSGTKKEIRPVTIATYQVLTTRRK--GVYPHLE 284 Query: 311 LFNERNWGLIIYDEVHLLPAPVFRATADIQATRRLGLXXXATLVREDGREDDVFSLIGPK 370 LF+ R+WGLI+YDEVHLLPAPVF+ TAD+QA RRLGL ATLVREDGRE DVFSLIGPK Sbjct: 285 LFDSRDWGLIVYDEVHLLPAPVFKFTADLQARRRLGLT--ATLVREDGRESDVFSLIGPK 342 Query: 371 CYELPWKVLEKQGWIAAVDCIEVVVPMGTDIRHRYMYAGAKEQFRVXXXXXXXXXXXXXX 430 ++ PWK +E QG+IA DC+EV V + R Y A +E++R Sbjct: 343 RFDAPWKEIEAQGYIAPADCVEVRVNLTETERLAYATAETEEKYRFCATTATKRKVTEAL 402 Query: 431 XXXXXXXXVLVIGQYLDQLKQLAVSLGAPLITGKTSQQERNELYAAFNEGAIRLLVVSKV 490 +LVIGQY+DQL +L L AP+I G+TS +R +L+ AF EG I +LVVSKV Sbjct: 403 VRKFAGQQILVIGQYIDQLDELGEHLNAPVIKGETSNAQREKLFDAFREGEISVLVVSKV 462 Query: 491 ANFAVNLPDASVAIEVSGAYGSRQEEAQRLGRILRPKPGENKAYFYTLVSGDSREQDFAL 550 ANF+++LP+A+VAI+VSG +GSRQEEAQRLGR+LRPK ++A+FY++V+ D+ +QDFA Sbjct: 463 ANFSIDLPEATVAIQVSGTFGSRQEEAQRLGRVLRPKADGHQAHFYSVVARDTIDQDFAA 522 Query: 551 RRRLFLTEQGYDYAV 565 R+ FL EQGY Y + Sbjct: 523 HRQRFLAEQGYAYRI 537 >ref|NP_737501.1| putative DNA helicase [Corynebacterium efficiens YS-314] dbj|BAC17701.1| putative DNA helicase [Corynebacterium efficiens YS-314] Length = 557 Score = 476 bits (1226), Expect = e-133, Method: Compositional matrix adjust. Identities = 260/556 (46%), Positives = 345/556 (62%), Gaps = 17/556 (3%) Query: 10 GKGTCIIRKDRTILLECGHPGFEAARTALADFAELVKSPPAYHTYRITQLSLWNAAAAGK 69 G G I++ D+T+LLE GH AR ALA FAEL ++P HTYRIT L+LWNA AAG Sbjct: 4 GDGPLIVQSDKTVLLETGHDLAGEARAALAPFAELERAPEHVHTYRITPLALWNARAAGH 63 Query: 70 TAQEILAVLSLLSRCGIPAGLEEEVRQLVSRYGRLELHSGADGGSLMVLRADNPVXXXXX 129 A++++ VL SR +P L +V + +SRYGR+ +H G ++L + P Sbjct: 64 DAEQVVDVLERYSRFPVPQPLLVDVAETMSRYGRVRMHKHPAHG--LILESSEPAILVEI 121 Query: 130 XXXXXXXXXXXNRTAPLECRCPAEQRGLLKQELARLGFPVLDYAGYRDGQALNVPWKKAA 189 + P +RG LKQEL ++G+P D AGY DG+A + A Sbjct: 122 SRHRKIKPMLGQQLDPETIAVHPSERGRLKQELLKIGWPAEDLAGYVDGEAHPI-----A 176 Query: 190 GSGAVYGESVGLRDYQEEAVRRFXXXXXXXXXXXXXLPCGAGKTLVGLAVLESLQCETLI 249 S V E LRDYQ+ A F LPCGAGKT+VG A + Q TLI Sbjct: 177 LSTEV--EDWSLRDYQQYAADSFWEGGSGVVV----LPCGAGKTMVGAASMARAQATTLI 230 Query: 250 LTSNATSVAQWKAELLKRTGLDEEAVGEYSGVKREVRPITVATYQILTHRRSKGGPFGHM 309 L +N + QWK ELL+RT L E+ +GEYSG ++E+RP+T+ATYQ++T RR+KG + + Sbjct: 231 LVTNTVAGRQWKDELLRRTTLTEDEIGEYSGERKEIRPVTIATYQVVT-RRTKG-EYKAL 288 Query: 310 NLFNERNWGLIIYDEVHLLPAPVFRATADIQATRRLGLXXXATLVREDGREDDVFSLIGP 369 LF+ R+WGLIIYDEVHLLPAPVFR T+D+Q+ RRLGL ATLVREDGRE DVFSLIGP Sbjct: 289 ELFDSRDWGLIIYDEVHLLPAPVFRMTSDLQSRRRLGLT--ATLVREDGREGDVFSLIGP 346 Query: 370 KCYELPWKVLEKQGWIAAVDCIEVVVPMGTDIRHRYMYAGAKEQFRVXXXXXXXXXXXXX 429 K Y+ PWK LE QG+IA DC+EV M R Y A +++R+ Sbjct: 347 KRYDAPWKDLEAQGFIATADCVEVRTTMTDAERMVYATAETADRYRLAATAHTKTRVVRR 406 Query: 430 XXXXXXXXXVLVIGQYLDQLKQLAVSLGAPLITGKTSQQERNELYAAFNEGAIRLLVVSK 489 L+IG YLDQL++L AP++ GKTS ++R EL+ F G + +LVVSK Sbjct: 407 ILEQHQGQPTLIIGAYLDQLEELGAEFDAPIVDGKTSNKKRGELFDRFRSGELNVLVVSK 466 Query: 490 VANFAVNLPDASVAIEVSGAYGSRQEEAQRLGRILRPKPGENKAYFYTLVSGDSREQDFA 549 VANF+++LP+A+VAI+VSG +GSRQEEAQRLGR+LRPK +A+FY++VS D+ + ++A Sbjct: 467 VANFSIDLPEAAVAIQVSGTFGSRQEEAQRLGRLLRPKADGGEAHFYSVVSRDTLDTEYA 526 Query: 550 LRRRLFLTEQGYDYAV 565 R+ FL EQGY Y + Sbjct: 527 AHRQRFLAEQGYAYRI 542 >ref|NP_600045.1| putative helicase [Corynebacterium glutamicum ATCC 13032] dbj|BAB98209.1| DNA or RNA helicases of superfamily II [Corynebacterium glutamicum ATCC 13032] emb|CAF19522.1| DNA or RNA helicase of superfamily II [Corynebacterium glutamicum ATCC 13032] Length = 557 Score = 474 bits (1221), Expect = e-132, Method: Compositional matrix adjust. Identities = 255/556 (45%), Positives = 345/556 (62%), Gaps = 17/556 (3%) Query: 10 GKGTCIIRKDRTILLECGHPGFEAARTALADFAELVKSPPAYHTYRITQLSLWNAAAAGK 69 G G I++ D+T+LLE HP AR ALA FAEL ++P HTYRIT L+LWNA AAG Sbjct: 4 GDGPLIVQSDKTVLLEIDHPQAGEARIALAPFAELERAPEHIHTYRITPLALWNARAAGH 63 Query: 70 TAQEILAVLSLLSRCGIPAGLEEEVRQLVSRYGRLELHSGADGGSLMVLRADNPVXXXXX 129 A++++ +L SR +P L ++ + +SRYGR+ LH G ++L + P Sbjct: 64 DAEQVVDMLERFSRFPVPQALLIDIAETMSRYGRVRLHRHPAHG--LILESGEPAILVEI 121 Query: 130 XXXXXXXXXXXNRTAPLECRCPAEQRGLLKQELARLGFPVLDYAGYRDGQALNVPWKKAA 189 + P +RG LKQEL ++G+P D AGY DG++ + Sbjct: 122 SRHKKIKPMLGAQVDPETIVVHPSERGRLKQELLKVGWPAEDLAGYVDGESHPI------ 175 Query: 190 GSGAVYGESVGLRDYQEEAVRRFXXXXXXXXXXXXXLPCGAGKTLVGLAVLESLQCETLI 249 G + E LRDYQ+ A F LPCGAGKT+VG A + Q TLI Sbjct: 176 GLSTEF-EDWSLRDYQQMAADSFWEGGSGVVV----LPCGAGKTMVGAASMARAQATTLI 230 Query: 250 LTSNATSVAQWKAELLKRTGLDEEAVGEYSGVKREVRPITVATYQILTHRRSKGGPFGHM 309 L +N + QWK ELL+RT L E+ +GEYSG ++E+RP+T+ATYQ++T RR+KG + + Sbjct: 231 LVTNTVAGRQWKDELLRRTTLTEDEIGEYSGERKEIRPVTIATYQVVT-RRTKG-EYKAL 288 Query: 310 NLFNERNWGLIIYDEVHLLPAPVFRATADIQATRRLGLXXXATLVREDGREDDVFSLIGP 369 LF+ R+WGLIIYDEVHLLPAPVFR T+D+Q+ RRLGL ATLVREDGRE DVFSLIGP Sbjct: 289 ELFDSRDWGLIIYDEVHLLPAPVFRMTSDLQSRRRLGLT--ATLVREDGREGDVFSLIGP 346 Query: 370 KCYELPWKVLEKQGWIAAVDCIEVVVPMGTDIRHRYMYAGAKEQFRVXXXXXXXXXXXXX 429 K Y+ PWK LE QG+IA DC+E+ M R Y A + +++R+ Sbjct: 347 KRYDAPWKDLESQGFIATADCVEIRSTMTDAERMVYATAESADRYRLAATAHTKVAVVRK 406 Query: 430 XXXXXXXXXVLVIGQYLDQLKQLAVSLGAPLITGKTSQQERNELYAAFNEGAIRLLVVSK 489 L+IG YLDQL++L AP+I GKT ++R L+ F G++ +LVVSK Sbjct: 407 LLEEHAGKPTLIIGAYLDQLEELGAEFNAPVIDGKTPNKKREALFDQFRSGSLSVLVVSK 466 Query: 490 VANFAVNLPDASVAIEVSGAYGSRQEEAQRLGRILRPKPGENKAYFYTLVSGDSREQDFA 549 VANF+++LP+ASVAI+VSG +GSRQEEAQRLGR+LRPK ++A+FY++VS D+ + ++A Sbjct: 467 VANFSIDLPEASVAIQVSGTFGSRQEEAQRLGRLLRPKHDGSEAHFYSIVSRDTLDTEYA 526 Query: 550 LRRRLFLTEQGYDYAV 565 R+ FL EQGY Y + Sbjct: 527 AHRQRFLAEQGYAYRI 542 >ref|NP_488743.1| DNA repair helicase [Nostoc sp. PCC 7120] pir||AG2393 DNA repair helicase [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB76402.1| DNA repair helicase [Nostoc sp. PCC 7120] Length = 563 Score = 473 bits (1216), Expect = e-132, Method: Compositional matrix adjust. Identities = 258/564 (45%), Positives = 345/564 (61%), Gaps = 10/564 (1%) Query: 6 MNGSGKGTCIIRKDRTILLECGHPGFEAARTALADFAELVKSPPAYHTYRITQLSLWNAA 65 M+ + + + I++ DRTILLE P AR A+A FAEL+KSP HTY+IT LS+WNA Sbjct: 1 MSYNPENSLIVQSDRTILLEVHSPTAAKAREAIAPFAELIKSPEHIHTYQITPLSIWNAR 60 Query: 66 AAGKTAQEILAVLSLLSRCGIPAGLEEEVRQLVSRYGRLELHSGADGGSLMVLRADNPVX 125 AAG + ++A L ++ IP + +E+ L RYG + D + LR + Sbjct: 61 AAGMQVEAMVAALRDYAKYPIPEAVAQEISTLGERYGLTVIERDEDS---LRLRVSDVAL 117 Query: 126 XXXXXXXXXXXXXXXNRTAPLECRCPAEQRGLLKQELARLGFPVLDYAGYRDGQALNVPW 185 R + + + A RG LKQ L +G+P D AGY G AL++ Sbjct: 118 AELLVRDKQVAPLLGKRLSEVSFQVEAGLRGTLKQALLAVGYPAEDIAGYVSGDALSIKL 177 Query: 186 KKAAGSGAVYGESVGLRDYQEEAVRRFXXXXXXXXXX-XXXLPCGAGKTLVGLAVLESLQ 244 ++ +G + LRDYQ +A F LPCGAGKT+VG++ + ++Q Sbjct: 178 REETRTGKKFL----LRDYQRQAAEAFYQSGRVQGGSGVIVLPCGAGKTIVGMSAIAAVQ 233 Query: 245 CETLILTSNATSVAQWKAELLKRTGLDEEAVGEYSGVKREVRPITVATYQILTHRRSKGG 304 TLIL+++ TSV QW+ ELL +T L EEA+ EYSG + PIT++TYQILT+R ++ Sbjct: 234 ENTLILSTSLTSVRQWRRELLDKTDLPEEAIAEYSGEVKNTGPITLSTYQILTYRPNRED 293 Query: 305 PFGHMNLFNERNWGLIIYDEVHLLPAPVFRATADIQATRRLGLXXXATLVREDGREDDVF 364 F H +LF+ R+WGLIIYDEVHLLPAPVFR TA++QA RRLGL ATL+REDGRE DVF Sbjct: 294 DFPHFDLFSARSWGLIIYDEVHLLPAPVFRITAELQARRRLGLT--ATLIREDGREGDVF 351 Query: 365 SLIGPKCYELPWKVLEKQGWIAAVDCIEVVVPMGTDIRHRYMYAGAKEQFRVXXXXXXXX 424 +LIGPK Y++PW+ LE +G+IA C E+ V + + Y A + QFRV Sbjct: 352 ALIGPKRYDVPWRELETEGFIATASCTEIRVSQDAERQMTYALAPRRNQFRVAAENPHKV 411 Query: 425 XXXXXXXXXXXXXXVLVIGQYLDQLKQLAVSLGAPLITGKTSQQERNELYAAFNEGAIRL 484 +L+IG++L QL+ LA G PLITGKT ++ER +LY AF EG + Sbjct: 412 QVVKELLEKESGHRILIIGEFLAQLETLAKVTGLPLITGKTPEKEREQLYQAFREGKLGG 471 Query: 485 LVVSKVANFAVNLPDASVAIEVSGAYGSRQEEAQRLGRILRPKPGENKAYFYTLVSGDSR 544 LV+S+V NFA++LPDA + I+VSG YGSRQEEAQRLGR+LRPK A FYTLVS + Sbjct: 472 LVLSRVGNFAIDLPDADILIQVSGKYGSRQEEAQRLGRVLRPKSDGRPAQFYTLVSLRTC 531 Query: 545 EQDFALRRRLFLTEQGYDYAVQTV 568 E+DFA R+LFLTEQGY Y +Q V Sbjct: 532 EEDFARHRQLFLTEQGYSYNIQIV 555 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 743,524,906 Number of extensions: 27674865 Number of successful extensions: 59782 Number of sequences better than 1.0e-05: 100 Number of HSP's gapped: 59466 Number of HSP's successfully gapped: 131 Length of query: 575 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 444 Effective length of database: 401,559,609 Effective search space: 178292466396 Effective search space used: 178292466396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)