BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0064.p (621 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO47386.1| cyclodextrinase enzyme; CDase; 78 kD [Paenibacill... 623 e-177 pdb|1EA9|C Chain C, Cyclomaltodextrinase >gi|21730148|pdb|1EA9|D... 615 e-174 pdb|1SMA|A Chain A, Crystal Structure Of A Maltogenic Amylase >g... 608 e-172 gb|AAA92925.1| cyclomaltodextrinase 607 e-172 dbj|BAB63955.1| alpha-cyclodextrinase [Geobacillus stearothermop... 606 e-172 >gb|AAO47386.1| cyclodextrinase enzyme; CDase; 78 kD [Paenibacillus sp. A11] Length = 653 Score = 623 bits (1607), Expect = e-177, Method: Compositional matrix adjust. Identities = 294/588 (50%), Positives = 396/588 (67%), Gaps = 7/588 (1%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EA++H P NW+Y Y+ T HLR+RT K ++ V+AL GDKY W+H E M K+A+D+ Sbjct: 4 EAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLATDE 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE V P +R Y F +Q G E WMTE F TE PA P+ +E P+I+ D+F+ Sbjct: 64 LFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANPDRLFEYPFINPVDVFQ 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLDYLAR 204 P W K A+FYQI P+RFANGD DP WG PT FFGGDLQG++DHLD+L++ Sbjct: 124 PPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDHLSK 183 Query: 205 LGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNH 264 LG+ A+Y TP+F+A +NHKYDT DY ++DP FGD + LKKLV H +GIRV+LDAVFNH Sbjct: 184 LGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAVFNH 243 Query: 265 ISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEAR 324 P F V+++GE+S+Y WFHI + P++V DG P YD F F MPKLNT +PE + Sbjct: 244 SGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHPEVK 303 Query: 325 KYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWL 384 +YLL A YW+ E GIDGWRLDVANE+ H FWR+FR++VK NP+A+I+G+VW +S WL Sbjct: 304 EYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGDVWHESSIWL 363 Query: 385 NGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTP 444 GDQFD+VMNYP T+ ++++ + +A +F LG L YP+QA+EV+FNLL SHDT Sbjct: 364 EGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSHDTA 423 Query: 445 RILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDL 504 R+LTQ GD K+KLA F FT+ GTPCI+YGDE+GL+G DP CRKCM W+ + ++DL Sbjct: 424 RLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHDKDL 483 Query: 505 FESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRL 564 F YQ++IRLR+A AL+TG F+ L+A+ R Y R D+ ++ +N + TL L Sbjct: 484 FAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHTLTL 543 Query: 565 PLED-RFQDALNGEKLIPSDGQMVLDLAPYSYKILIKETD-----RPP 606 P+ ++ N + L + GQ+ + L Y + +L +D RPP Sbjct: 544 PVRHAQWTHLWNDDVLTAAHGQLTVKLPAYGFAVLKASSDWSSWGRPP 591 >pdb|1EA9|C Chain C, Cyclomaltodextrinase pdb|1EA9|D Chain D, Cyclomaltodextrinase Length = 583 Score = 615 bits (1586), Expect = e-174, Method: Compositional matrix adjust. Identities = 289/580 (49%), Positives = 392/580 (67%), Gaps = 2/580 (0%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EA++H P N++Y Y+ T HLR+RT K ++ V+AL GDKY W+H E M K+A+D+ Sbjct: 4 EAVYHRPRKNFSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLATDE 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE V P +R Y F +Q G E WMTE F TE PA P+ +E P+I+ D+F+ Sbjct: 64 LFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANPDRLFEYPFINPVDVFQ 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLDYLAR 204 P W K A+FYQI P+RFANGD DP WG PT FFGGDLQG++DHLD+L++ Sbjct: 124 PPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDHLSK 183 Query: 205 LGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNH 264 LG+ A+Y TP+F+A +NHKYDT DY ++DP FGD + LKKLV H +GIRV+LDAVFNH Sbjct: 184 LGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAVFNH 243 Query: 265 ISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEAR 324 P F V+++GE+S+Y WFHI + P++V DG P YD F F MPKLNT +P+ + Sbjct: 244 SGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHPDVK 303 Query: 325 KYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWL 384 +YLL A YW+ E GIDGWRLDVANE+ H FWR+FR++VK NP+A+I+GEVW +S WL Sbjct: 304 EYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGEVWHESSIWL 363 Query: 385 NGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTP 444 GDQFD+VMNYP T+ ++++ + +A +F LG L YP+QA+EV+FNLL SHDT Sbjct: 364 EGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSHDTA 423 Query: 445 RILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDL 504 R+LTQ GD K+KLA F FT+ GTPCI+YGDE+GL+G DP CRKCM W+ + ++DL Sbjct: 424 RLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHDKDL 483 Query: 505 FESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRL 564 F YQ++IRLR+A AL+TG F+ L+A+ R Y R D+ ++ +N + TL L Sbjct: 484 FAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHTLTL 543 Query: 565 PLED-RFQDALNGEKLIPSDGQMVLDLAPYSYKILIKETD 603 P+ ++ + L + GQ+ + L Y + +L +D Sbjct: 544 PVRHAQWTHLWQDDVLTAAHGQLTVKLPAYGFAVLKASSD 583 >pdb|1SMA|A Chain A, Crystal Structure Of A Maltogenic Amylase pdb|1SMA|B Chain B, Crystal Structure Of A Maltogenic Amylase gb|AAC15072.1| maltogenic amylase [Thermus sp. IM6501] Length = 588 Score = 608 bits (1569), Expect = e-172, Method: Compositional matrix adjust. Identities = 296/588 (50%), Positives = 387/588 (65%), Gaps = 9/588 (1%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEH---YHEEHRMQ 79 +++EA+HH N+AY YD +T HLR++T K +VD V L GD Y+W + M+ Sbjct: 1 MRKEAIHHRSTDNFAYAYDSETLHLRLQTKKNDVDHVELLFGDPYEWHDGAWQFQTMPMR 60 Query: 80 KIASDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYY-ERPYI 138 K SD FDYW VKP +R Y F ++AG E L TE GF+ E P+ YY P++ Sbjct: 61 KTGSDGLFDYWLAEVKPPYRRLRYGFVLRAGGEKLVYTEKGFYHEAPSDDTAYYFCFPFL 120 Query: 139 HEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWG-DTPTAESFFGGDLQGIMD 197 H DLF+AP+W K V+YQI P+RFANG+P P WG + PT SFFGGDLQGI+D Sbjct: 121 HRVDLFQAPDWVKDTVWYQIFPERFANGNPAISPKGARPWGSEDPTPTSFFGGDLQGIID 180 Query: 198 HLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVV 257 HLDYLA LGIT IYLTPIF APSNHKYDT DY ++DP+FGD E LK LV H KGIRV+ Sbjct: 181 HLDYLADLGITGIYLTPIFRAPSNHKYDTADYFEIDPHFGDKETLKTLVKRCHEKGIRVM 240 Query: 258 LDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLN 317 LDAVFNH + FQ V+++G S Y WFHI FP++ T+ +PNYD F F MPKLN Sbjct: 241 LDAVFNHCGYEFAPFQDVLKNGAASRYKDWFHIREFPLQ-TEPRPNYDTFAFVPHMPKLN 299 Query: 318 TTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVW 377 T +PE ++YLLD A YW+ E IDGWRLDVANEIDH FWR+FR+ VK + P+ +I+GE+W Sbjct: 300 TAHPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHQFWREFRQAVKALKPDVYILGEIW 359 Query: 378 SDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNL 437 D+M WL GDQFD+VMNYPL + + D++A F +L ++L YP+Q NE FNL Sbjct: 360 HDAMPWLRGDQFDAVMNYPLADAALRFFAKEDMSASEFADRLMHVLHSYPKQVNEAAFNL 419 Query: 438 LASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEG 497 L SHDTPR+LT GGD K+KL F TFTG+PCI+YGDEIG+ G DP+CRKCM+W+ Sbjct: 420 LGSHDTPRLLTVCGGDVRKVKLLFLFQLTFTGSPCIYYGDEIGMTGGNDPECRKCMVWDP 479 Query: 498 ERQNRDLFESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSE 557 E+QN++L+E + LI LRK + AL+ G L+AD + VY + D N +I +N S Sbjct: 480 EKQNKELYEHVKQLIALRKQYRALRRGDVAFLTADDEVNHLVYAKTDGNETVMIIINRSN 539 Query: 558 QPATLRLPLEDR---FQDALNGEKLIPSDGQMVLDLAPYSYKILIKET 602 + A + +P++ R + L GE+ + + L PY + + E+ Sbjct: 540 EAAEIPMPIDARGKWLVNLLTGERFAAEAETLCVSLPPYGFVLYAVES 587 >gb|AAA92925.1| cyclomaltodextrinase Length = 558 Score = 607 bits (1564), Expect = e-172, Method: Compositional matrix adjust. Identities = 281/540 (52%), Positives = 373/540 (69%), Gaps = 1/540 (0%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EA++H P NW+Y Y+ T HLR+RT K ++ V+AL GDKY W+H E M K+A+D+ Sbjct: 4 EAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLATDE 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE V P +R Y F +Q G E WMTE F TE P P+ +E P+I+ D+F+ Sbjct: 64 LFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPRNPDRLFEYPFINPVDVFQ 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLDYLAR 204 P W K A+FYQI P+RFANGD DP WG PT FFGGDLQG++DHLD+L++ Sbjct: 124 PPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDHLSK 183 Query: 205 LGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNH 264 LG+ A+Y TP+F+A +NHKYDT DY ++DP FGD + LKKLV H +GIRV+LDAVFNH Sbjct: 184 LGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAVFNH 243 Query: 265 ISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEAR 324 P F V+++GE+S+Y WFHI + P++V DG P YD F F MPKLNT +P+ + Sbjct: 244 SGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHPDVK 303 Query: 325 KYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWL 384 +YLL A YW+ E GIDGWRLDVANE+ H FWR+FR++VK NP+A+I+GEVW +S WL Sbjct: 304 EYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGEVWHESSIWL 363 Query: 385 NGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTP 444 GDQFD+VMNYP T+ ++++ + +A +F LG L YP+QA+EV+FNLL SHDT Sbjct: 364 EGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSHDTA 423 Query: 445 RILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDL 504 R+LTQ GD K+KLA F FT+ GTPCI+YGDE+GL+G DP CRKCM W+ + ++DL Sbjct: 424 RLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHDKDL 483 Query: 505 FESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRL 564 F YQ++IRLR+A AL+TG F+ L+A+ R Y R D+ ++ +N + TLR Sbjct: 484 FAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHTLRC 543 >dbj|BAB63955.1| alpha-cyclodextrinase [Geobacillus stearothermophilus] Length = 588 Score = 606 bits (1562), Expect = e-172, Method: Compositional matrix adjust. Identities = 295/588 (50%), Positives = 386/588 (65%), Gaps = 9/588 (1%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEH---YHEEHRMQ 79 +++EA+HH ++AY YD +T HLR++T K +VD V L GD Y+W + M+ Sbjct: 1 MRKEAIHHRSTDHFAYAYDSETLHLRLQTKKHDVDHVELLFGDPYEWHDGAWQFQTMPMR 60 Query: 80 KIASDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYY-ERPYI 138 K SD FDYW VKP +R Y F ++AGDE L TE GF+ E P+ YY P++ Sbjct: 61 KTGSDGLFDYWLAEVKPPYRRLRYGFVLRAGDEKLVYTEKGFYHEAPSDDTAYYFCFPFL 120 Query: 139 HEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWG-DTPTAESFFGGDLQGIMD 197 H DLF+AP+W K V+YQI P+RFANG+P P WG + PT SFFGGDLQGI+D Sbjct: 121 HRVDLFQAPDWVKDTVWYQIFPERFANGNPAISPKGARPWGSEDPTPTSFFGGDLQGIID 180 Query: 198 HLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVV 257 HLDYLA LGIT IYLTPIF APSNHKYDT DY ++DP+FGD E LK LV H KGIRV+ Sbjct: 181 HLDYLADLGITGIYLTPIFRAPSNHKYDTADYFEIDPHFGDKETLKTLVKRCHEKGIRVM 240 Query: 258 LDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLN 317 LDAVFNH + FQ V+++G S Y WFHI FP++ T+ +PNYD F F MPKLN Sbjct: 241 LDAVFNHCGYEFAPFQDVLKNGAASRYKDWFHIREFPLQ-TEPRPNYDTFAFVPQMPKLN 299 Query: 318 TTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVW 377 T +PE ++YLLD A YW+ E IDGWRLDVANEIDH FWR+FR+ VK + P+ +I+GE+W Sbjct: 300 TAHPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHQFWREFRQAVKALKPDVYILGEIW 359 Query: 378 SDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNL 437 D+M WL GDQFD+VMNYP T + + +I+A +F + LL YP+ NE FNL Sbjct: 360 HDAMPWLRGDQFDAVMNYPFTDGALRFFAKEEISARQFADIMVRLLHSYPKHVNEAAFNL 419 Query: 438 LASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEG 497 L SHDTPR+LT GGD K KL F TFTG+PCI+YGDEIG+ G DP+CRKCM+W+ Sbjct: 420 LGSHDTPRLLTVCGGDVRKAKLLFLFQLTFTGSPCIYYGDEIGMTGGNDPECRKCMVWDT 479 Query: 498 ERQNRDLFESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSE 557 E+QN++L+E + LI LR+ + AL+ G L+AD + VYE+ D N +I +N S Sbjct: 480 EKQNKELYEHVKQLIALRRQYRALRRGDVAFLAADDEANHLVYEKTDGNETVMIIINRSN 539 Query: 558 QPATLRLPLEDR---FQDALNGEKLIPSDGQMVLDLAPYSYKILIKET 602 + A + +P++ R + L GE+ + + L PY + + E+ Sbjct: 540 EAAEIPMPIDARGKWLVNLLTGERFAAEAETLCVSLPPYGFVLYAVES 587 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,045,130,376 Number of extensions: 47679063 Number of successful extensions: 96629 Number of sequences better than 1.0e-05: 1042 Number of HSP's gapped: 94435 Number of HSP's successfully gapped: 1078 Length of query: 621 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 489 Effective length of database: 398,844,510 Effective search space: 195034965390 Effective search space used: 195034965390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)