BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0029.p (144 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_622029.1| Phosphotransferase system mannitol/fructose-spe... 132 3e-30 sp|P17876|PTMA_STACA PTS system, mannitol-specific IIA component... 132 3e-30 sp|Q45420|PTMA_BACST PTS system, mannitol-specific IIA component... 130 1e-29 ref|NP_693522.1| PTS system mannitol-specific enzyme II A compon... 122 3e-27 ref|NP_372682.1| PTS system, mannitol specific IIA component [St... 120 7e-27 >ref|NP_622029.1| Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Thermoanaerobacter tengcongensis] gb|AAM23633.1| Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Thermoanaerobacter tengcongensis] Length = 146 Score = 132 bits (332), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 60/142 (42%), Positives = 95/142 (66%) Query: 3 ILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIPH 62 IL+E +++N ++ K EAI G LLV+ G+V +EY+ M +REE ++TY+G G+AIPH Sbjct: 5 ILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGVAIPH 64 Query: 63 GTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDDA 122 G E +IK +G+ + ++PDGVDFG A++VIGIA GD H+ +L+ +A+ ++ Sbjct: 65 GVSEYVKYIKKSGIVIAQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQYEEN 124 Query: 123 IERVMNAPTPADVIAIFEGGLE 144 +E++ A TP ++I I E G E Sbjct: 125 VEKLKKAKTPQEIIEILEKGDE 146 >sp|P17876|PTMA_STACA PTS system, mannitol-specific IIA component (EIIA-Mtl) (Mannitol-permease IIA component) (Phosphotransferase enzyme II, A component) (EIII-Mtl) pir||JQ0088 phosphotransferase system enzyme II (EC 2.7.1.69), mannitol-specific, factor III - Staphylococcus carnosus gb|AAA26656.1| mannitol-specific enzyme-III Length = 144 Score = 132 bits (331), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 60/138 (43%), Positives = 99/138 (71%), Gaps = 1/138 (0%) Query: 3 ILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIPH 62 + S + +N + +D+ EAI AG+ LVDAG VTE+Y+ M +RE VVST+MG GLAIPH Sbjct: 4 LFSNENIFLNQSFEDQNEAIEKAGQALVDAGAVTEDYIQAMKDREAVVSTFMGNGLAIPH 63 Query: 63 GTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDDA 122 GT EA+ + +GL++++ P+GV + GD+ A VV+GIA H+++L+ +A+ F+E++ Sbjct: 64 GTDEAKSAVLQSGLTLLQIPEGVQW-GDDVAKVVVGIAGKDGEHLDLLSKIAITFSEEEN 122 Query: 123 IERVMNAPTPADVIAIFE 140 ++R++N +P ++ A+FE Sbjct: 123 VDRIVNTKSPEEIKAVFE 140 >sp|Q45420|PTMA_BACST PTS system, mannitol-specific IIA component (EIIA-Mtl) (Mannitol-permease IIA component) (Phosphotransferase enzyme II, A component) (EIII-Mtl) gb|AAC44465.1| mannitol enzyme IIA [Geobacillus stearothermophilus] Length = 145 Score = 130 bits (326), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 56/139 (40%), Positives = 98/139 (70%) Query: 1 MSILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAI 60 M IL + ++++ ++K EAI +AG++LV+ G+V + Y+ KM ERE + STYMG +AI Sbjct: 3 MPILKKENIVLHARVENKTEAIRLAGQILVNNGYVEDSYIDKMFEREALTSTYMGNFIAI 62 Query: 61 PHGTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTED 120 PHGT++A+ F+K +G+S+I+ PDGVDFG ++IGIA + H+E+L+ +A++ +E Sbjct: 63 PHGTEDAKQFVKHSGISIIQIPDGVDFGDGNIVKLLIGIAGKNNEHLEILSKIAIVCSEV 122 Query: 121 DAIERVMNAPTPADVIAIF 139 + +E ++ A T ++++I Sbjct: 123 ENVETMIKAATEEEILSIL 141 >ref|NP_693522.1| PTS system mannitol-specific enzyme II A component [Oceanobacillus iheyensis HTE831] dbj|BAC14557.1| PTS system, mannitol-specific enzyme II, A component [Oceanobacillus iheyensis HTE831] Length = 145 Score = 122 bits (306), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 57/138 (41%), Positives = 90/138 (65%) Query: 3 ILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIPH 62 ILS+ + + K EAI G++L G+V + Y+ KMLEREE+ ST+MG LAIPH Sbjct: 5 ILSKENIQLGVEFSSKEEAIRYTGEILFRNGYVEQTYIEKMLEREELTSTFMGNALAIPH 64 Query: 63 GTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDDA 122 GT++AR + TGLSV+ P G+DFG ++IGIA GD H+E+L+ +A+I +E + Sbjct: 65 GTEDAREMVIDTGLSVVTVPKGIDFGEGNQVQILIGIAGKGDEHLEILSQIAIICSEVEN 124 Query: 123 IERVMNAPTPADVIAIFE 140 +E+++ A + +V+ + + Sbjct: 125 VEKLVAASSKEEVLELLQ 142 >ref|NP_372682.1| PTS system, mannitol specific IIA component [Staphylococcus aureus subsp. aureus Mu50] ref|NP_375272.1| PTS system, mannitol specific IIA component [Staphylococcus aureus subsp. aureus N315] ref|NP_646901.1| PTS system mannitol specific IIA component [Staphylococcus aureus subsp. aureus MW2] sp|P17875|PTMA_STAAM PTS system, mannitol-specific IIA component (EIIA-Mtl) (Mannitol-permease IIA component) (Phosphotransferase enzyme II, A component) (EIII-Mtl) pir||B90011 PTS system, mannitol specific IIA component [imported] - Staphylococcus aureus (strain N315) emb|CAB55331.1| putative mannitol-specific IIA component [Staphylococcus aureus] dbj|BAB43251.1| PTS system, mannitol specific IIA component [Staphylococcus aureus subsp. aureus N315] dbj|BAB58320.1| PTS system, mannitol specific IIA component [Staphylococcus aureus subsp. aureus Mu50] dbj|BAB95949.1| PTS system mannitol specific IIA component [Staphylococcus aureus subsp. aureus MW2] Length = 144 Score = 120 bits (302), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 56/138 (40%), Positives = 94/138 (68%), Gaps = 1/138 (0%) Query: 3 ILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIPH 62 + S + + +N + EAI AGK LVD+G VT+ Y+ M +RE+VVST+MG GLAIPH Sbjct: 4 LFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAMKDREQVVSTFMGNGLAIPH 63 Query: 63 GTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDDA 122 GT EA+ + +GL++++ P+GVD+ G E VV+GIA H+++L+ +A+ F+E++ Sbjct: 64 GTDEAKTNVIHSGLTLLQIPEGVDWDG-EVVKVVVGIAGKDGEHLDLLSKIAITFSEEEN 122 Query: 123 IERVMNAPTPADVIAIFE 140 ++R++ A + ++ +FE Sbjct: 123 VDRIVQAKSAEEIKQVFE 140 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 216,089,959 Number of extensions: 8821219 Number of successful extensions: 18887 Number of sequences better than 1.0e-05: 141 Number of HSP's gapped: 18959 Number of HSP's successfully gapped: 141 Length of query: 144 Length of database: 757,237,578 Length adjustment: 104 Effective length of query: 40 Effective length of database: 474,867,282 Effective search space: 18994691280 Effective search space used: 18994691280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)