BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0028.p (380 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q45421|MTLD_BACST Mannitol-1-phosphate 5-dehydrogenase >gi|14... 350 4e-95 ref|NP_784055.1| mannitol-1-phosphate 5-dehydrogenase [Lactobaci... 332 9e-90 ref|NP_244718.1| PTS system, mannitol-1-phosphate dehydrogenase ... 325 1e-87 ref|ZP_00037046.1| COG0246: Mannitol-1-phosphate/altronate dehyd... 318 2e-85 gb|EAJ02942.1| unknown [environmental sequence] 308 2e-82 >sp|Q45421|MTLD_BACST Mannitol-1-phosphate 5-dehydrogenase gb|AAC44466.1| mannitol-1-phosphate dehydrogenase [Geobacillus stearothermophilus] Length = 382 Score = 350 bits (897), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 171/370 (46%), Positives = 247/370 (66%), Gaps = 1/370 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LLSQ+GYEV FVD+N+ V L+E++EY V +A E ++ ++++NV+A++S T Sbjct: 13 RGFIGSLLSQSGYEVVFVDINDELVRLLKEKQEYRVIIADENRQELLIRNVSAVNSQTER 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 E+V I +A L+TTAVG IL IA ++A+G++KR+ ++ PLH+IACEN IGGS LK Sbjct: 73 EKVIDYITKAHLITTAVGPHILPAIATILAEGLQKRITINKTPLHIIACENMIGGSDVLK 132 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 V+ K+ + + F N AVDRIVP Q+H+D L VVVEPF+EWVI+ +I Sbjct: 133 SHVFEKISEADKPLFEKYYGFLNCAVDRIVPNQKHDDPLSVVVEPFFEWVIEKRNIIGAI 192 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 P+ G H+VD+L+PYIERKLFTVNTGH A+YLGY + +TIQ+AM D+ + + V + L Sbjct: 193 PPIQGAHFVDDLKPYIERKLFTVNTGHAIASYLGYYKKLQTIQEAMCDQEIRSDVEKALH 252 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 E+GAVL++K+G+++ +H YI KI++RF NP+++DEV RV RSP+RKL NDRL+ PA Q Sbjct: 253 ESGAVLVKKYGWNENEHQSYIQKIIQRFINPSISDEVVRVARSPIRKLGANDRLIGPATQ 312 Query: 312 AYDR-GLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQ 370 YD G L + +A LLFD ++D + +Y+ + DH + Sbjct: 313 YYDLFGQVPHGLVKGIAALLLFDYENDEEAVALQKTIQETGVEGALYQYSQLEKDHPLVI 372 Query: 371 SIMSEYAKLK 380 +I ++ LK Sbjct: 373 AIKDQWQHLK 382 >ref|NP_784055.1| mannitol-1-phosphate 5-dehydrogenase [Lactobacillus plantarum WCFS1] sp|Q88ZS1|MTLD_LACPL Mannitol-1-phosphate 5-dehydrogenase emb|CAD62894.1| mannitol-1-phosphate 5-dehydrogenase [Lactobacillus plantarum WCFS1] Length = 385 Score = 332 bits (851), Expect = 9e-90, Method: Compositional matrix adjust. Identities = 159/327 (48%), Positives = 224/327 (68%), Gaps = 1/327 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG L+ G+++ FVDVN+ + +L +R Y + LA+EGQ+ + V +V +++ Sbjct: 13 RGFIGETLADNGFKITFVDVNDTLIDELNKRNGYTIELAAEGQKHIEVHDVKGINNGKDP 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNA-PLHVIACENAIGGSAQL 130 + VA IA+AD+VTTA+G ILK IA ++ADG++ R A +N P+ +IACEN IGGS L Sbjct: 73 KAVAEEIAQADMVTTAIGPKILKFIAPLIADGLKLRQANNNTTPIDIIACENMIGGSQSL 132 Query: 131 KELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPG 190 K+ VY L+ + ++ AD + FPNAAVDRIVPLQ+H+D L V VEPF EWVID SQM Sbjct: 133 KKSVYESLNEDEQAWADQNAGFPNAAVDRIVPLQKHDDPLFVSVEPFKEWVIDKSQMKNP 192 Query: 191 YVPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVL 250 + + GV Y D+LEPYIERKLF+VNTGH + AY G ++GY+TI +A+ D+++ Q + VL Sbjct: 193 KIQLKGVDYADDLEPYIERKLFSVNTGHATVAYTGNMKGYKTIGEAVKDDSVVDQAKHVL 252 Query: 251 EETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPAL 310 ETG +LIQK GFD H Y KIL RF NP ++D++ RVGR+P+RKL N+R + P Sbjct: 253 GETGDLLIQKWGFDPEVHHAYQKKILSRFENPYISDDIERVGRTPIRKLGFNERFIRPIR 312 Query: 311 QAYDRGLGYTALTRSMAGALLFDVKDD 337 + +RG Y+AL ++ F+ +D Sbjct: 313 ELKERGRDYSALVDTVGEMFFFNYPND 339 >ref|NP_244718.1| PTS system, mannitol-1-phosphate dehydrogenase (enzyme III) [Bacillus halodurans] sp|Q9K681|MTLD_BACHD Mannitol-1-phosphate 5-dehydrogenase pir||C84131 PTS system, mannitol-1-phosphate dehydrogenase (enzyme III) mtlD [imported] - Bacillus halodurans (strain C-125) dbj|BAB07570.1| PTS system, mannitol-1-phosphate dehydrogenase (enzyme III) [Bacillus halodurans] Length = 374 Score = 325 bits (833), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 171/362 (47%), Positives = 222/362 (61%), Gaps = 3/362 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LLS+A YEV FVDVN + +L E++ Y V A E E ++ NV L+S T Sbjct: 13 RGFIGALLSKANYEVVFVDVNAQVIDRLNEQRSYTVLTADEDNEENVIHNVRGLNSRTQM 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 E+V A IA ADLVTTAVG ++L H+A + G+ +R + AP+ VIACENAIG S+ L+ Sbjct: 73 EQVLAEIATADLVTTAVGPSVLPHLAHPIGQGLLQR---NGAPIQVIACENAIGASSMLQ 129 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 E A L E SK D FPNA VDRIVP Q H D L V VEPFYEWVI++ M Sbjct: 130 EYTKASLSEEEWSKVDRVTGFPNATVDRIVPAQDHADPLTVSVEPFYEWVIETKSMKGEP 189 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 + GV YV++L PYIERKLFTVNTGH AYLG+ +G TI++A++D+ + + R+ L Sbjct: 190 PTIDGVTYVEDLTPYIERKLFTVNTGHAMVAYLGFQKGLMTIKEAISDQTIAEKTRQALA 249 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 ET +L+ K+ F H +YI+KI +R+ NP L+D V RVGR+P+RKL N+RLV PA Q Sbjct: 250 ETKGLLVHKYNFSPEAHDEYIEKIFKRYNNPYLSDRVERVGRNPIRKLGYNERLVKPARQ 309 Query: 312 AYDRGLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQS 371 D G TAL + A F V+DD V + TG+ A H + Q Sbjct: 310 LLDLGHQPTALLAGIQAAFAFFVEDDQESMELQEKRQVQGLEQTVVEVTGLPAVHPLVQM 369 Query: 372 IM 373 I+ Sbjct: 370 IV 371 >ref|ZP_00037046.1| COG0246: Mannitol-1-phosphate/altronate dehydrogenases [Enterococcus faecium] Length = 383 Score = 318 bits (814), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 166/326 (50%), Positives = 216/326 (66%), Gaps = 5/326 (1%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG +L+ G+E+ FVDVN + L ERKEY + LA E +E + VKNV +++ T+ Sbjct: 13 RGFIGEVLAANGFEITFVDVNHTIIDALNERKEYEIELADESKEKITVKNVKGINNQTNP 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAV-SNAPLHVIACENAIGGSAQL 130 E+V IAEADLVTTA+G IL IA ++A GI++R S L +IACEN IGGSA L Sbjct: 73 EKVIQEIAEADLVTTAIGPNILPFIAELIAKGIQEREKEGSTTSLDIIACENMIGGSAFL 132 Query: 131 KELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPG 190 ++ VY L S D + FPNAAVDRIVPLQ HED L V VEPF EWVID SQ Sbjct: 133 EKEVYKYLP--ETSFTDKYIGFPNAAVDRIVPLQHHEDPLFVQVEPFKEWVIDDSQRKNK 190 Query: 191 YVPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVL 250 + + GV YVD+LEPYIERKLF+VNTGH + AY G L GY+TI +AM D + AQ++ VL Sbjct: 191 KIQLKGVLYVDDLEPYIERKLFSVNTGHATVAYTGALLGYQTIDEAMQDALVVAQLKSVL 250 Query: 251 EETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPAL 310 +ETG++LI K GFD QH YI+KI+ RF+N ++D ++RV R+PLRKL +R P Sbjct: 251 QETGSLLIAKWGFDAEQHHAYIEKIIHRFQNKNISDAITRVARTPLRKLGYQERFTRPVR 310 Query: 311 QAYDRGLGYTALTRSMAGALLFDVKD 336 + + L LT +M ++F+ D Sbjct: 311 ELQEHNLTCPHLTATM--GIIFNYYD 334 >gb|EAJ02942.1| unknown [environmental sequence] Length = 381 Score = 308 bits (788), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 169/372 (45%), Positives = 227/372 (61%), Gaps = 6/372 (1%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LL+ A ++V F DVNE + QL R+EY V + Q+ +V+NV A+SS H Sbjct: 13 RGFIGKLLADASHQVTFADVNETLIDQLNHRQEYKVHVVGADQKLDVVRNVAAVSSAGH- 71 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSN-APLHVIACENAIGGSAQL 130 EV A I ADLVTTAVG IL IA +A G++ R N PL+VIACEN + G++ L Sbjct: 72 -EVIARIITADLVTTAVGPNILDKIASTLAKGLQARFDAGNLTPLNVIACENMVRGTSHL 130 Query: 131 KELVYAKLDGESRSKADVSVAFPNAAVDRIVP-LQQHEDILKVVVEPFYEWVIDSSQMIP 189 K+ V L + D + F ++AVDRIVP ++D L+V VE F EW++D +Q Sbjct: 131 KQEVLKYLPVAYHATLDACIGFVDSAVDRIVPPAAANDDPLEVTVESFSEWIVDQTQF-K 189 Query: 190 GYVP-VAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVRE 248 G +P VAG+ DNL ++ERKLFT+NTGH AYLG LRGY TI++A+ D + ++VR Sbjct: 190 GELPQVAGMEPTDNLMAFVERKLFTLNTGHIVTAYLGKLRGYRTIREAIEDPLIRSKVRR 249 Query: 249 VLEETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSP 308 +EE+GAVL+Q++GFD H+ YI+KIL RF NP L DE+ RVGR PLRKL+ DRLV P Sbjct: 250 AMEESGAVLVQRYGFDPRLHAAYIEKILARFANPYLVDEIDRVGRQPLRKLAAGDRLVKP 309 Query: 309 ALQAYDRGLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTV 368 L + GL L +A AL + DDP + + TG+AAD + Sbjct: 310 LLGTLEYGLPSDHLQEGIAAALHYRNADDPQAVELQQLLAELGPAQALARITGLAADSDI 369 Query: 369 HQSIMSEYAKLK 380 +I++ Y LK Sbjct: 370 VSAIVTRYGNLK 381 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 475,641,709 Number of extensions: 17847658 Number of successful extensions: 48526 Number of sequences better than 1.0e-05: 188 Number of HSP's gapped: 48097 Number of HSP's successfully gapped: 189 Length of query: 380 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 253 Effective length of database: 412,420,005 Effective search space: 104342261265 Effective search space used: 104342261265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)