BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0045.p (387 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00084177.1| COG2807: Cyanate permease [Pseudomonas fluore... 169 9e-41 ref|NP_743131.1| cyanate MFS transporter [Pseudomonas putida KT2... 166 1e-39 ref|NP_470283.1| hypothetical transport protein [Listeria innocu... 157 3e-37 ref|NP_391928.1| similar to ABC transporter (permease) [Bacillus... 157 4e-37 ref|ZP_00125585.1| COG2807: Cyanate permease [Pseudomonas syring... 153 6e-36 >ref|ZP_00084177.1| COG2807: Cyanate permease [Pseudomonas fluorescens PfO-1] Length = 434 Score = 169 bits (428), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 106/344 (30%), Positives = 181/344 (52%), Gaps = 6/344 (1%) Query: 16 NLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTIFF 75 NLRP ++S+A LLG + LG++A A LLTTLPVLC+G+F+P+A +L+ R G ER + Sbjct: 61 NLRPALSSMAPLLGEVSKSLGLSAAQAGLLTTLPVLCLGLFAPLAPLLARRFGAERVVLG 120 Query: 76 SLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPD-IVSIYSI 134 L + +LR S + L +++GG I + G +L +K+ F + + +Y++ Sbjct: 121 ILLTLAGGIILRSSFGEIG-LFAGSVLGGASIGIIGVLLPGIVKRDFAKHAGTMTGVYTM 179 Query: 135 SMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIRQR 194 ++ +GAA+A+G T+P+ H +++ + L W I +A + W+ + KH N R R Sbjct: 180 ALCLGAAMAAGSTVPLSEHFDHSWAMGLGFWVIPALVAAVFWLPQIGQKHGAHNVAYRVR 239 Query: 195 LPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQIPIAFL 254 + + A Q T++ GL +S+ Y + W+ I + G + + + L+ +IQ+ + Sbjct: 240 GLLRDPLAWQVTLYMGLQSSLAYIVFGWLPSILIGRGLTPTQAGLVLSGSVIIQLASSLA 299 Query: 255 VPGSVKRSGKPKXXXXXXXXXXXIG--VGLLLFQFPMLLA-VLFLGIGAGGLFPLALMLP 311 P R GK + +G G L L + LG+G GG F LAL L Sbjct: 300 APWLATR-GKDQRLAIVVVMALTLGGLFGCLFAPIEGLWGWAILLGLGQGGTFSLALTLI 358 Query: 312 ISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNS 355 + ++ + A S+MSQ GY + + GP +G + D +G +N+ Sbjct: 359 VLRSRDSHVAANLSSMSQGFGYTLASMGPFAVGVVHDWTGGWNA 402 >ref|NP_743131.1| cyanate MFS transporter [Pseudomonas putida KT2440] gb|AAN66595.1| cyanate MFS transporter [Pseudomonas putida KT2440] Length = 434 Score = 166 bits (419), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 109/358 (30%), Positives = 186/358 (51%), Gaps = 6/358 (1%) Query: 18 RPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTIFFSL 77 RP ++S+A +LG + LG+NA A LLTTLPVLC+G+F+P+A VL+ R G ER I L Sbjct: 63 RPALSSMAPVLGQVSEGLGLNASQAGLLTTLPVLCLGLFAPLAPVLARRFGSERVILGIL 122 Query: 78 FLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPD-IVSIYSISM 136 + L V+R + ++ + L +L+ G I + G +L +K+ FP+ + +Y++++ Sbjct: 123 LTLALGLVVRSTFGAIGVFL-GSLMAGASIGIIGVLLPGIVKRDFPQHAGTLTGVYTMAL 181 Query: 137 TIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIRQRLP 196 +GAA+A+G T+P+ H + + + L W + +A+L W+ R H R R Sbjct: 182 CLGAAMAAGATVPLAQHFDGSWALGLGFWVLPALLAMLVWLPQARQGHGLHKVAYRVRGL 241 Query: 197 IANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQIPIAFLVP 256 + A Q T++ GL +S+ Y + W+ I + G + + + L+ ++Q+ + P Sbjct: 242 WRDPLAWQVTLYMGLQSSLAYIVFGWLPSILIGRGLTPTEAGLVLSGSVIVQLISSLSAP 301 Query: 257 GSVKRSGKPKXXXXXXXXXXXIGV-GLLLFQFPMLLA-VLFLGIGAGGLFPLALMLPISE 314 R + G+ G L L + LG+G GG F LAL L + Sbjct: 302 WLATRGKDQRLAIVVVMSGTLAGLFGCLYAPLSGLWGWAIVLGLGQGGTFALALTLIVLR 361 Query: 315 TKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNS--SIFAMLIIIMVMIGV 370 +K A A S+M+Q GY + + GP +G + D +G + + IFA+L + V+ G+ Sbjct: 362 SKDAHVAANLSSMAQGVGYTLASMGPFAVGLVHDLTGGWTALGWIFAVLGVGAVVFGL 419 >ref|NP_470283.1| hypothetical transport protein [Listeria innocua] pir||AI1550 hypothetical transport protein lin0946 [imported] - Listeria innocua (strain Clip11262) emb|CAC96177.1| lin0946 [Listeria innocua] Length = 405 Score = 157 bits (398), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 111/363 (30%), Positives = 192/363 (52%), Gaps = 9/363 (2%) Query: 14 AMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTI 73 A NLR ITSV LLG IQS L + A LLTT+P+L SP + LS +G+E I Sbjct: 27 AANLRAPITSVGPLLGMIQSDLHLTNTMAGLLTTIPLLAFAGLSPFVSKLSRSLGIEVLI 86 Query: 74 FFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPDIVS-IY 132 F +L + + ++LR L T ++ G+ I++ +L S IKK FP K +++ +Y Sbjct: 87 FIALLTLVIGSLLRPFGGEFYLFAGTFMI-GLAIAVGNVILPSLIKKEFPFKLGLMTGVY 145 Query: 133 SISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIR 192 SISM + AALA+G T+P+ +++++ ++ WA+LG +AL+ W+ +R + Sbjct: 146 SISMNLCAALAAGLTVPIAMNNKFSWHGSMVFWAMLGVLALVIWLPQLRYNQRSSEKIAT 205 Query: 193 QRLPIANKK---AIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQI 249 + + K A + +++ G +++FY +W+ I G S SS + L++ + Sbjct: 206 HLVTTSVWKSPLAWKISLYMGSQSAIFYISIAWLPNILADQGLSSTSSGLMLSLMQFAVM 265 Query: 250 PIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQ----FPMLLAVLFLGIGAGGLFP 305 PI F++P R + IG+ ++F F + A++ GIG+G F Sbjct: 266 PITFIIPIIAGRMKNQQLLVGISVLLFMIGIFGIMFASGNLFILATAIILYGIGSGMSFS 325 Query: 306 LALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAMLIIIM 365 L++M T +A E+ S M+Q GY+ A GP+L G + D G++ +++ ++ I + Sbjct: 326 LSMMFFNLRTTSATESADLSGMAQSIGYLFAASGPILFGTLHDALGSWQPTLYVLIGITL 385 Query: 366 VMI 368 +M+ Sbjct: 386 IML 388 >ref|NP_391928.1| similar to ABC transporter (permease) [Bacillus subtilis] sp|P37482|YYCB_BACSU Hypothetical transport protein yycB pir||S65973 transport protein homolog yycB - Bacillus subtilis dbj|BAA05179.1| unknown [Bacillus subtilis] emb|CAB16085.1| yycB [Bacillus subtilis subsp. subtilis str. 168] Length = 402 Score = 157 bits (397), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 121/359 (33%), Positives = 189/359 (52%), Gaps = 6/359 (1%) Query: 14 AMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTI 73 A NLRP ITSV ++ +I++ L ++ A LT LP+L + SP+A L R+G ERT+ Sbjct: 22 AFNLRPAITSVGPVISSIRAELHMSNGAAGFLTALPLLSFAVLSPLAPKLGQRLGNERTL 81 Query: 74 FFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPDI-VSIY 132 + L ++ + + R + + L TAL+ G+GI++ +L S IK +PEKP I +S+Y Sbjct: 82 WLGLVILLIGVLTRSTGYTAALFFGTALI-GVGIAIGNVLLPSLIKHKYPEKPGIMISLY 140 Query: 133 SISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIR 192 + SM I AALASG ++P+ T A W L +ALL WI +R++ Sbjct: 141 TTSMNIFAALASGVSVPLATQMNGGWKQAFLLWGGLALLALLIWIPQLRHRDTANQTMKL 200 Query: 193 QRLPI-ANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQIPI 251 Q I A+K A TIF GL + +FYS +W I S G ++ +++ +P Sbjct: 201 QSSSIWASKMAWYVTIFMGLQSFLFYSSIAWFPEILRSHGIDTATAGWMVSLMQFASLPS 260 Query: 252 AFLVPGSVKRSGKPKXXXXXXXXXXXIGV-GLLLFQFPMLLAV--LFLGIGAGGLFPLAL 308 FL P R + + IG+ GLL LLA+ + +GIG G LAL Sbjct: 261 TFLTPVLADRVKQQRGIVAALASVYLIGLCGLLAGGSHTLLAIWMIIIGIGQGSSISLAL 320 Query: 309 MLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAMLIIIMVM 367 L ++ A++A S MSQ GY++ A GP+ +G++ DQ+ ++ I ++ ++VM Sbjct: 321 TLIGLRSENAQQAAALSGMSQSFGYLLAAVGPIFVGYLFDQTHSWTMPIVLLIAALIVM 379 >ref|ZP_00125585.1| COG2807: Cyanate permease [Pseudomonas syringae pv. syringae B728a] Length = 414 Score = 153 bits (387), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 107/349 (30%), Positives = 176/349 (50%), Gaps = 6/349 (1%) Query: 18 RPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIGLERTIFFSL 77 RP ++S+A LL T+ LG++A A LLTTLPVLC+G+F+P+A +L+ R G ER + L Sbjct: 43 RPALSSIAPLLDTVSDSLGMSAAEAGLLTTLPVLCLGLFAPLAPILARRFGSERVVMVVL 102 Query: 78 FLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPD-IVSIYSISM 136 + +R V L +L+ G I + G +L +K+ FP K + +Y++++ Sbjct: 103 LTLAAGLAVRSLFGQVG-LFGGSLLAGASIGIIGVLLPGIVKRDFPHKAGAMTGVYTMAL 161 Query: 137 TIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQTNATIRQRLP 196 +GAA+A+G T+P+ + + I L W + IA W R H R R Sbjct: 162 CLGAAIAAGSTVPLSQYVGGSWQIGLGFWLVPALIAAAFWFPQAREVHGTHRDVYRVRGL 221 Query: 197 IANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIFTLIQIPIAFLVP 256 + + A Q T++ GL +S+ Y + W+ I + G + + + L+ + Q+ A VP Sbjct: 222 LRDPLAWQVTLYMGLQSSLSYIVFGWLPSILIGRGLTPTQAGLLLSGSIIAQVISALAVP 281 Query: 257 GSVKRSGKPKXXXXXXXXXXXIGV-GLLLFQFPMLLA-VLFLGIGAGGLFPLALMLPISE 314 R + IG+ G L L + LGIG GG F LAL L + Sbjct: 282 YLATRGKDQRLAIMLVMSLTLIGLFGCLYAPLGGLWGWAILLGIGQGGTFSLALALIVLR 341 Query: 315 TKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNS--SIFAML 361 ++ + A S+M+Q GY + + GP +G + D +G++++ IFA+L Sbjct: 342 SRDSHVAANLSSMAQGVGYTIASTGPFAVGVVHDMTGSWSAIGWIFAVL 390 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 496,799,929 Number of extensions: 18956654 Number of successful extensions: 84636 Number of sequences better than 1.0e-05: 108 Number of HSP's gapped: 84479 Number of HSP's successfully gapped: 110 Length of query: 387 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 260 Effective length of database: 412,420,005 Effective search space: 107229201300 Effective search space used: 107229201300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)