BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0018.p (358 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388609.1| similar to CDP-glucose 4,6-dehydratase [Bacillu... 253 7e-66 gb|EAD88423.1| unknown [environmental sequence] 207 3e-52 gb|EAI01557.1| unknown [environmental sequence] 200 4e-50 gb|EAK40535.1| unknown [environmental sequence] 188 2e-46 gb|EAF51537.1| unknown [environmental sequence] 174 4e-42 >ref|NP_388609.1| similar to CDP-glucose 4,6-dehydratase [Bacillus subtilis] pir||B69815 CDP-glucose 4,6-dehydratase homolog yfnG - Bacillus subtilis dbj|BAA20116.1| YfnG [Bacillus subtilis] emb|CAB12547.1| yfnG [Bacillus subtilis subsp. subtilis str. 168] Length = 301 Score = 253 bits (645), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 132/306 (43%), Positives = 196/306 (64%), Gaps = 8/306 (2%) Query: 42 LTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADYNLIERAITDGKINTIF 101 + LIEQGA VT ++ + P+S + KK+ GS+ D +IERA+ + +I+T+F Sbjct: 1 MVKELIEQGANVTGLVRDHVPQSNLYQGEHIKKMNIVRGSLEDLAVIERALGEYEIDTVF 60 Query: 102 HLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKIIVASSDKVYGDSPDLPY 161 HLAA ++ +A + P TFE N+ GT+N+LE CR H I ++IVASSDK YGD +LPY Sbjct: 61 HLAAQAIVGVANRNPISTFEANILGTWNILEACRKH-PLIKRVIVASSDKAYGDQENLPY 119 Query: 162 DEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFGGGDLNFRRLIPGTIQR 221 DE MP+QG++PYD SKSC+DLIS +Y H++ LP+ I R GN++GGGDLNF R+IP TIQ Sbjct: 120 DENMPLQGKHPYDVSKSCADLISHTYFHTYGLPVCITRCGNLYGGGDLNFNRIIPQTIQL 179 Query: 222 LYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMYHYV-NNEGTGNVFNFGTGKLWD 280 + P IR S+G + YI+D + AY+ + + N G FNF Sbjct: 180 VLNGEAPEIR--SDG---TFVRDYFYIEDAVQAYLLLAEKMEENNLAGEAFNFSNEIQLT 234 Query: 281 IKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSALNWSAETSLEEGIRKTVD 340 + +++E I + MN +++P +Q ++EI HQYLS EKA+ LNW+ +++EG+ KT++ Sbjct: 235 VLELVEKILKKMN-SNLKPKVLNQGSNEIKHQYLSAEKARKLLNWTPAYTIDEGLEKTIE 293 Query: 341 WYHNYW 346 WY ++ Sbjct: 294 WYTEFF 299 >gb|EAD88423.1| unknown [environmental sequence] Length = 266 Score = 207 bits (527), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 102/258 (39%), Positives = 159/258 (61%), Gaps = 6/258 (2%) Query: 86 NLIERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKII 145 N ++ +I + +I+ ++HL A ++ + A P TFE+N++GTYNLLE C + + I+ Sbjct: 1 NFLKSSIKEHEIDIVYHLGAQAIVETAANNPLTTFESNIRGTYNLLEACHSSEQNLKAIV 60 Query: 146 VASSDKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFG 205 VASSDK YG +LPY E + G PY+ SKSC+DL+S+SY ++++PI I R GN++G Sbjct: 61 VASSDKAYGTHGNLPYTETTSLNGIYPYEVSKSCTDLLSRSYAITYDMPIAIARCGNVYG 120 Query: 206 GGDLNFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAM-YHYVNN 264 G DLN+ R++PGTI LY PIIR S+G ++Y++D++ AY+++ ++ Sbjct: 121 GADLNWNRIVPGTINDLYNDRSPIIR--SDG---THLRDYIYVKDIVDAYISLSLATLSG 175 Query: 265 EGTGNVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSALN 324 G FNFG + +++ I +M HI+P E+ EI QYL + KAK LN Sbjct: 176 IPNGEAFNFGNNNPVSVLDLVDKISGLMGKNHIKPTIENSAIGEIHSQYLDSSKAKQMLN 235 Query: 325 WSAETSLEEGIRKTVDWY 342 W SLE+G+ +T++WY Sbjct: 236 WEPNYSLEKGLTETIEWY 253 >gb|EAI01557.1| unknown [environmental sequence] Length = 324 Score = 200 bits (509), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 109/317 (34%), Positives = 181/317 (57%), Gaps = 9/317 (2%) Query: 30 LVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADYNLIE 89 L+TG+SG +G+W+ L+ A + + + + + + K + +Y I++++ +E Sbjct: 13 LITGSSGLLGSWMVDKLLSLDANLIGIAKDKSMNTLLESKNILKGFKTFYLDISEFSKVE 72 Query: 90 RAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKIIVASS 149 + ++ +I+ FHLAA + A P +TF++N++GT+NLLE+ R+ I+VASS Sbjct: 73 KVFSENEIDITFHLAAQTQVIDALINPLETFKSNIEGTWNLLELSRLFD---RPIVVASS 129 Query: 150 DKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFGGGDL 209 DK YG S LPY E P++G PY+ SKS +DL+S++Y ++NLP+V R GNIFGGGDL Sbjct: 130 DKAYGSSEKLPYKENYPLEGEFPYEVSKSTTDLLSKTYSSTYNLPVVTLRCGNIFGGGDL 189 Query: 210 NFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAM-YHYVNNEGTG 268 NF RLIPG I L PI+R +NG ++Y++D++ AY+ + N+ Sbjct: 190 NFDRLIPGVINWLLNDEKPILR--TNG---DYIREWVYVEDVVNAYLGTGKALLENKNNH 244 Query: 269 NVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSALNWSAE 328 + +NF +G+ + ++ EI+ + IEP ++ EI +Q L + LN + Sbjct: 245 SAYNFSSGEKKSVIEIYEILCNKICGNFIEPEFKLSSEKEIKNQELDSSLINDDLNIYSL 304 Query: 329 TSLEEGIRKTVDWYHNY 345 L E + +T+ WY Y Sbjct: 305 YQLNESLDETISWYKEY 321 >gb|EAK40535.1| unknown [environmental sequence] Length = 350 Score = 188 bits (477), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 118/348 (33%), Positives = 196/348 (56%), Gaps = 18/348 (5%) Query: 21 KSSLSDCRALVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYG 80 KS + L+TG +GF+G+ L+ L+ GA++ + ++ S + K I+ +Y Sbjct: 7 KSYWKNKTVLITGINGFIGSNLSKLLLSLGAKIVGIANDKKKNSLLEYYAIKKDIKIHYL 66 Query: 81 SIADYNLIERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQ 140 ++DY ++ I + +I+T HLAA ++A P+ TFE+N++GTYNLLE+ R HQ Sbjct: 67 DLSDYKKLDTIIKNYQIHTCIHLAAQVDVNVASVNPFLTFESNIRGTYNLLELLR-HQKS 125 Query: 141 ISKIIVASSDKVYGD--SPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHS-FNLPIVI 197 I I+VASSDK YG + +LPY E ++ + PYD SK+ D+I++SY F +PI+I Sbjct: 126 IKSIVVASSDKAYGHYATKELPYKEDYDLRAKYPYDVSKAAGDMIAKSYASDMFKMPIMI 185 Query: 198 GRFGNIFGGGDLNFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMA 257 RF NI+G G LNF LIP I + I R SNG FL+++D+ Y+ Sbjct: 186 TRFTNIYGPGQLNFTALIPDCILSCFNYRKFIPR--SNG---MNKRDFLFVEDVCDLYLC 240 Query: 258 MYHYVNNEGT--GNVFNFGTGKLWDIKKVIEII----QRVMNLEHIEPLYEHQKN-SEIL 310 + + + + + G VFN GTG + +K +++ I N + I P ++++K+ EI Sbjct: 241 LSYNLAKDKSLRGEVFNAGTGSGYKVKDIVKKICIKSNNNENYQSILPRFKNKKSFGEIK 300 Query: 311 HQYLSTEKAKSALNWSAETSLEEGIRKTVDWYHNYWNSDSQNYESALN 358 +Q+++ +K W S+++GI KT+ WY ++ +Y+S L+ Sbjct: 301 NQFMTFDKINKYFKWKPRYSIDDGIHKTIIWYTDFLK--KHDYKSFLS 346 >gb|EAF51537.1| unknown [environmental sequence] Length = 235 Score = 174 bits (440), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 90/196 (45%), Positives = 124/196 (63%), Gaps = 3/196 (1%) Query: 28 RALVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADYNL 87 + LVTG +GFVG L LI QGAEV ++ N S LDKKI+ + G + D +L Sbjct: 9 KVLVTGINGFVGGNLCKGLINQGAEVFGLIRNKNYESFLYYENLDKKIKIFEGDLCDKDL 68 Query: 88 IERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKIIVA 147 R I++ +IN IFHLAA + P+ TFE+N++GTY+LLE CR+ D + II+A Sbjct: 69 FSRIISEEQINNIFHLAAQVEVGVGISNPYLTFESNIRGTYSLLEACRLFPDNLESIIIA 128 Query: 148 SSDKVYGDSP--DLPYDEGMPIQGRNPYDASKSCSDLISQSYRHS-FNLPIVIGRFGNIF 204 SSDK YG P +PY E P+ + PYD SK+C+DL++QSY + + LPI++ RF NI+ Sbjct: 129 SSDKSYGSYPVDMMPYKEDYPLLPKFPYDTSKACADLVAQSYANDIYKLPIIVTRFCNIY 188 Query: 205 GGGDLNFRRLIPGTIQ 220 G G LNF +IP I+ Sbjct: 189 GPGQLNFSAVIPDGIR 204 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 537,111,348 Number of extensions: 22094097 Number of successful extensions: 70870 Number of sequences better than 1.0e-05: 2575 Number of HSP's gapped: 67887 Number of HSP's successfully gapped: 2579 Length of query: 358 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 231 Effective length of database: 412,420,005 Effective search space: 95269021155 Effective search space used: 95269021155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)