BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0011.p (378 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAF71997.1| alpha-glucosidase [Bacillus sp. DG0303] 432 e-120 gb|EAJ76917.1| unknown [environmental sequence] 422 e-117 ref|NP_388662.1| trehalose-6-phosphate hydrolase [Bacillus subti... 421 e-116 ref|NP_241738.1| alpha,alpha-phosphotrehalase [Bacillus halodura... 412 e-114 emb|CAA56495.1| phospho alpha (1-1) glucosidase [Bacillus subtil... 410 e-113 >gb|AAF71997.1| alpha-glucosidase [Bacillus sp. DG0303] Length = 562 Score = 432 bits (1112), Expect = e-120, Method: Compositional matrix adjust. Identities = 199/377 (52%), Positives = 271/377 (71%), Gaps = 3/377 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW +KGVDGFR+DV+NL+SKDQ F R+FYTDGPR+HE++ E+ +VF Sbjct: 186 MHFWFQKGVDGFRLDVVNLLSKDQRFLDDDGSMPPGDGRKFYTDGPRIHEFLHEMNREVF 245 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+++TVGEMSSTT++HCI+Y+ PE RE +M FNFHHLKVDY NG+KW + +DF LK Sbjct: 246 SKYDVMTVGEMSSTTIDHCIKYTNPERRELNMVFNFHHLKVDYPNGEKWAVADFDFLALK 305 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ M + GGWNALFW NHDQPR +SR+ DDGKY KESAKMLAT +H MQGTPY+ Sbjct: 306 RILSEWQVEMHKGGGWNALFWCNHDQPRIVSRYGDDGKYHKESAKMLATVIHMMQGTPYI 365 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GM +P + I ++ D+ES NMY IL+E+G S ++ L+I++ +SRDNSRTPMQWD Sbjct: 366 YQGEEIGMTDPKFERIDDYRDVESLNMYHILREQGKSEQEVLEILKRKSRDNSRTPMQWD 425 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S +AGFT+G PW++V Y++INV+ DP S+F HY++LI+LRKQ +IT G ++ L Sbjct: 426 DSENAGFTTGKPWIRVAPNYQQINVKKALEDPTSVFYHYQRLIQLRKQYDIITTGDYQLL 485 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 E HP IFAY R G E L+VV++F G + F D+ +N A+E+LI N + +P Sbjct: 486 LEDHPDIFAYLRNGENEKLLVVNNFYGRETTFILPDDV---DVNGYASEILISNYDDSPS 542 Query: 361 WSQSLEIPPYGSYMWII 377 + + + PY S ++ + Sbjct: 543 DFRKITLRPYESIVYYL 559 >gb|EAJ76917.1| unknown [environmental sequence] Length = 577 Score = 422 bits (1086), Expect = e-117, Method: Compositional matrix adjust. Identities = 203/375 (54%), Positives = 260/375 (69%), Gaps = 10/375 (2%) Query: 3 FWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVFGP 62 FWA+KGVDGFR+DVINLISKDQ+F RRFYTDGPR+HE+++++ VF P Sbjct: 212 FWAKKGVDGFRLDVINLISKDQAFPNDEIGDG----RRFYTDGPRIHEFLQDVSRDVFAP 267 Query: 63 YNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELKEL 122 +TVGEMSST+LEHC RY A + E SM FNFHHLKVDY NG KW P+DF ELK + Sbjct: 268 VGAMTVGEMSSTSLEHCQRYGALDGSELSMVFNFHHLKVDYPNGDKWTKAPFDFLELKRI 327 Query: 123 FTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYVYQ 182 F WQ+GM GW+ALFW NHDQPR +SRF D+G++R +AKMLA+TLHG+QGTPY+YQ Sbjct: 328 FNHWQSGMH-GKGWSALFWCNHDQPRIVSRFGDEGEHRVVAAKMLASTLHGLQGTPYIYQ 386 Query: 183 GEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWDAS 242 GEE+GM NP + I ++ D+ES+N++ I Q G+S + L I+ +SRDNSRTPMQW A+ Sbjct: 387 GEEIGMTNPGYQRIDDYQDVESRNIFAIKQTEGMSKAEILAILGAKSRDNSRTPMQWSAA 446 Query: 243 GHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRLDE 302 +AGFT GTPWLK Y IN +A D S+F HY+ LIRLRK P+ T G ++ L Sbjct: 447 ANAGFTQGTPWLKPAANYPLINAEAALADQHSVFWHYRDLIRLRKTHPIFTQGDYQELLA 506 Query: 303 GHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPVWS 362 GHPQI+AYAR N +TL+VVS+F G A F+ DE + + LL+GN +P Sbjct: 507 GHPQIWAYARRANGQTLLVVSNFYGEPATFALPDELATGK-----GRLLLGNYPDSPARP 561 Query: 363 QSLEIPPYGSYMWII 377 QS + PY S +W++ Sbjct: 562 QSCMLRPYESLIWLV 576 >ref|NP_388662.1| trehalose-6-phosphate hydrolase [Bacillus subtilis] sp|P39795|TREC_BACSU Trehalose-6-phosphate hydrolase (Alpha,alpha-phosphotrehalase) pir||B69725 alpha,alpha-phosphotrehalase (EC 3.2.1.93) treA - Bacillus subtilis dbj|BAA23408.1| TreC [Bacillus subtilis] emb|CAB12610.1| trehalose-6-phosphate hydrolase [Bacillus subtilis subsp. subtilis str. 168] Length = 561 Score = 421 bits (1082), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/375 (53%), Positives = 268/375 (71%), Gaps = 8/375 (2%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW EKG+DGFR+DVINLISKDQ F R FYTDGPRVHE++ E+ KVF Sbjct: 189 MHFWFEKGIDGFRLDVINLISKDQRFPNAEEGDG----RSFYTDGPRVHEFLHEMNEKVF 244 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+ +TVGEMSSTT++HCIRY+ P+ +E MTF+FHHLKVDY NG+KW L P+DF +LK Sbjct: 245 SHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYPNGEKWALAPFDFLKLK 304 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 E+ + WQTGM GGWNALFW NHDQPR +SR+ DDG YR +SAKMLAT +H MQGTPY+ Sbjct: 305 EILSDWQTGMHAGGGWNALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQGTPYI 364 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEELGM NP + DIS + D+ES NMY +E+G++ + I++ +SRDNSRTP+QWD Sbjct: 365 YQGEELGMTNPKFTDISSYRDVESLNMYHAFKEKGMADQDITAILQAKSRDNSRTPVQWD 424 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 A+ + GFT+GTPW+ V Y+EIN +A D +S+F HY++LI++RK ++T+G ++ + Sbjct: 425 ATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGTYEII 484 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 + P IFAY R G+ E L+V+++F GT+A F+ D S + AE+L+ N E Sbjct: 485 AKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPD---SLAPDEWKAEVLLTNDEAREG 541 Query: 361 WSQSLEIPPYGSYMW 375 Q++ + PY S ++ Sbjct: 542 L-QNMTLRPYESIVY 555 >ref|NP_241738.1| alpha,alpha-phosphotrehalase [Bacillus halodurans] pir||H83758 alpha,alpha-phosphotrehalase treA [imported] - Bacillus halodurans (strain C-125) dbj|BAB04591.1| alpha,alpha-phosphotrehalase [Bacillus halodurans] Length = 559 Score = 412 bits (1060), Expect = e-114, Method: Compositional matrix adjust. Identities = 197/378 (52%), Positives = 259/378 (68%), Gaps = 3/378 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW KGVDGFR+DVINLISKDQ+F RRFYTDGPR HE++ E+ KVF Sbjct: 185 MDFWFTKGVDGFRLDVINLISKDQAFPNDDGSVPPGDGRRFYTDGPRAHEFMNEMNRKVF 244 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+ +TVGEMSSTTLEHCI+Y+ PE +E SMTFNFHHLKVDY NG+KW + DF LK Sbjct: 245 SKYDAMTVGEMSSTTLEHCIQYTRPENKELSMTFNFHHLKVDYPNGEKWAIGDMDFFALK 304 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 E+ ++WQ GM + GGWNALFW NHDQPR +SR+ +DGKY ++SAKMLATT+H MQGTPY+ Sbjct: 305 EILSKWQVGMHKGGGWNALFWCNHDQPRVVSRYGNDGKYHEKSAKMLATTIHMMQGTPYI 364 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE GM +P + I ++ D+E+ N Y IL+E+GLS E+ ++II+ +SRDNSRTP+QWD Sbjct: 365 YQGEEFGMTDPKFDRIDQYRDVETLNYYDILKEQGLSEEEVMEIIKRKSRDNSRTPVQWD 424 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFTSGTPW+ V Y+ IN + D SIF HYK+LI LRK ++T G ++ L Sbjct: 425 DSPHAGFTSGTPWIDVASNYRSINAKKALADKQSIFYHYKKLIELRKTYDIVTYGDYELL 484 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 P++FAY R ETL+VV+ F + ++ + + ++L+ N Sbjct: 485 LPEDPRVFAYVRRYQDETLLVVNHFFAGEVTIQVPEDVTAEK---HQVDVLLANYHDPSR 541 Query: 361 WSQSLEIPPYGSYMWIIR 378 Q L + PY S ++ ++ Sbjct: 542 EFQQLVLRPYESVVYYMK 559 >emb|CAA56495.1| phospho alpha (1-1) glucosidase [Bacillus subtilis] emb|CAA91015.1| TreA [Bacillus subtilis] Length = 555 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 192/356 (53%), Positives = 255/356 (71%), Gaps = 7/356 (1%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW EK +DGFR+DVINLISKDQ F R FYTDGPRVHE++ E+ KVF Sbjct: 188 MHFWFEKEIDGFRLDVINLISKDQRFPNAEEGDG----RSFYTDGPRVHEFLHEMNEKVF 243 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+ +TVGEMSSTT++HCIRY+ P+ +E MTF+FHHLKVDY +KW L P+DF +LK Sbjct: 244 SHYDSMTVGEMSSTTVDHCIRYTNPDNKELDMTFSFHHLKVDYLTAEKWALAPFDFLKLK 303 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 E+ + WQTGM GGWNALFW NHDQPR +SR+ DDG YR +SAKMLAT +H MQGTPY+ Sbjct: 304 EILSDWQTGMHAGGGWNALFWCNHDQPRVVSRYGDDGAYRVKSAKMLATAIHMMQGTPYI 363 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEELGM NP + DIS + D ES NMY +E+G++ + I++ +SRDNSRTP+QWD Sbjct: 364 YQGEELGMTNPKFTDISSYRDAESLNMYHAFKEKGMADQDITAILQAKSRDNSRTPVQWD 423 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 A+ + GFT+GTPW+ V Y+EIN +A D +S+F HY++LI++RK ++T+G ++ + Sbjct: 424 ATENGGFTTGTPWIPVAGNYREINAEAALRDQNSVFYHYQKLIQIRKMYDIVTEGTYEII 483 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTE 356 + P IFAY R G+ E L+V+++F GT+A F+ D S + AE+L+ N E Sbjct: 484 AKDDPNIFAYLRHGSNEKLLVINNFYGTEAAFTLPD---SLAPDEWKAEVLLTNDE 536 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 593,335,135 Number of extensions: 24889626 Number of successful extensions: 56527 Number of sequences better than 1.0e-05: 334 Number of HSP's gapped: 55767 Number of HSP's successfully gapped: 340 Length of query: 378 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 251 Effective length of database: 412,420,005 Effective search space: 103517421255 Effective search space used: 103517421255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)