BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0021.p (402 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|O52594|ACKA_CLOTM Acetate kinase (Acetokinase) >gi|2791839|gb... 480 e-134 ref|NP_971543.1| acetate kinase [Treponema denticola ATCC 35405]... 474 e-132 ref|NP_618481.1| acetate kinase [Methanosarcina acetivorans str.... 472 e-132 dbj|BAB71961.2| acetate kinase [Methanosarcina mazei] 462 e-129 sp|P38502|ACKA_METTE Acetate kinase (Acetokinase) >gi|538999|pir... 462 e-129 >sp|O52594|ACKA_CLOTM Acetate kinase (Acetokinase) gb|AAB96952.1| acetate kinase [Clostridium thermocellum] Length = 399 Score = 480 bits (1236), Expect = e-134, Method: Compositional matrix adjust. Identities = 225/400 (56%), Positives = 306/400 (76%), Gaps = 1/400 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 MNILVIN+GSSSLKYQL +MT+ESVLAKG+ +RIG++ S L H TG + V ++ H Sbjct: 1 MNILVINTGSSSLKYQLIDMTNESVLAKGVCDRIGLEHSFLKHTKTGGETVVIEKDLYNH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 AI++V++ LTD++ GVI S+ EI+AVGHR+VHGGE FK SA++D D IR +LA Sbjct: 61 KLAIQEVISALTDEKIGVIKSMSEISAVGHRIVHGGEKFKESAIIDEDVMKAIRDCVELA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP+ ++GI A + +P VP V VFDTAFHQTMP AY+YA+P +Y KYK+R+YG H Sbjct: 121 PLHNPSNIIGIEACKQILPDVPMVAVFDTAFHQTMPRHAYIYALPYEIYEKYKLRKYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V+ AA L +P+E LK+ITCH+GNGAS+ AV GG+S+DTSMG TPL+GL MGT Sbjct: 181 GTSHKYVAHRAAQMLGKPIESLKLITCHLGNGASICAVKGGKSVDTSMGFTPLQGLCMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 RSG++DPA++ Y+M KE++++ ++N+ LNK SG+L ISGVSSD RD+ D AEKG+ + L Sbjct: 241 RSGNVDPAVITYLMEKEKMNINDINNFLNKKSGVLGISGVSSDFRDVQDAAEKGDDRAQL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A D++ Y +RKYIG Y A +NGVD +VFTAG+GEN A +R +VL ++ F GI++D + N+ Sbjct: 301 ALDIFCYGVRKYIGKYIAVLNGVDAVVFTAGIGENNAYIRREVLKDMDFFGIKIDLDKNE 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQGI 400 V+ G IS ++KV+ LV+PTNEEL IAR+T R+V+ + Sbjct: 361 VK-GKEADISAPDAKVKTLVIPTNEELEIARETLRLVKNL 399 >ref|NP_971543.1| acetate kinase [Treponema denticola ATCC 35405] gb|AAS11424.1| acetate kinase [Treponema denticola ATCC 35405] Length = 397 Score = 474 bits (1220), Expect = e-132, Method: Compositional matrix adjust. Identities = 230/398 (57%), Positives = 294/398 (73%), Gaps = 1/398 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M ILVIN GSSSLKYQL +MT+E VL KGLVERIG++ S + H+ G V ++ +H Sbjct: 1 MKILVINCGSSSLKYQLIDMTNEDVLVKGLVERIGIEGSRIKHEKKGMDAVLIEEKMNDH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 AI+ VL L DK HGV+ S+DEI AVGHRVVHGGE F S L+D I++ DLA Sbjct: 61 KRAIQLVLEALIDKNHGVVKSMDEITAVGHRVVHGGEEFNKSVLLDDRVMAGIKECIDLA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNPA +MGI A + MP P V VFDTAFHQTMP + Y+YA+P Y KYK+RRYG H Sbjct: 121 PLHNPANIMGIEACKALMPKTPMVAVFDTAFHQTMPAENYIYALPYEYYEKYKIRRYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH FVS+ A++ L+ D K+ITCH+GNG+S+ A+ GG+ +DTSMG+TPLEGL+MGT Sbjct: 181 GTSHRFVSEKASETLKNNSADFKVITCHLGNGSSLAAIKGGKCVDTSMGLTPLEGLVMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 RSGDLDPAI+P++MNKE+LS E++++LNK SG+ ISGVSSD RDI A++G + L Sbjct: 241 RSGDLDPAILPFIMNKEKLSADEMSNVLNKKSGVFGISGVSSDFRDIETAAKEGNKRAQL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A D++ R+RKYI SYAA + GVD +VFTAG+GEN+ LR K+ + L FLG+ELD+E NK Sbjct: 301 ALDVFCNRVRKYIASYAAQLGGVDALVFTAGIGENSIELREKICHGLEFLGVELDSEKNK 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 VR G S SKV+VL++PTNEEL+IA+DT +V+ Sbjct: 361 VR-GKLTDASKASSKVKVLIIPTNEELMIAKDTMALVK 397 >ref|NP_618481.1| acetate kinase [Methanosarcina acetivorans str. C2A] sp|Q8TK19|ACKA_METAC Acetate kinase (Acetokinase) gb|AAM06961.1| acetate kinase [Methanosarcina acetivorans str. C2A] Length = 408 Score = 472 bits (1214), Expect = e-132, Method: Compositional matrix adjust. Identities = 220/398 (55%), Positives = 305/398 (76%), Gaps = 1/398 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M +LVIN+GSSSLKYQL +MT+ES LA GL ERIG+D+SI+ K + +++ + +++ H Sbjct: 1 MKVLVINAGSSSLKYQLIDMTNESPLAIGLCERIGIDNSIITQKRSDGKKLEKQTDLPNH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 A+ +V+ LTD E GVI S+DEINAVGHRVVHGGE F +SAL+D + I+ F+LA Sbjct: 61 KVALEEVVKALTDSELGVIKSMDEINAVGHRVVHGGEKFTSSALIDEGVEQAIKDCFELA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP +MGI+A + MPGVP V VFDTAFHQT+P AYMYA+P LY KY +R+YG H Sbjct: 121 PLHNPPNMMGITACQEIMPGVPMVAVFDTAFHQTIPAYAYMYALPYTLYEKYGIRKYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V++ AA L +P E++K+ITCH+GNG+S+TAV+GG+S++T+MG TPLEG+ MGT Sbjct: 181 GTSHFYVARRAAAMLGKPEEEVKVITCHLGNGSSITAVNGGKSVETTMGFTPLEGVAMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 R G +DPA+VP+VM KE L+ E+++++NK SG+L +SG+S+D RD+ + A KG + L Sbjct: 241 RCGSIDPAVVPFVMEKEGLTTREIDTLMNKKSGVLGVSGLSNDFRDLDEAASKGNQRAEL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A +++ Y+++K IG Y+A +NG D +VFTAG+GEN+A +R ++L+ L LGIE+D E NK Sbjct: 301 ALEIFAYKIKKVIGEYSAVLNGADAVVFTAGIGENSASIRKRILSGLDGLGIEIDEEKNK 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 +R G IST ++KV+VLV+PTNEEL IARDT I + Sbjct: 361 IR-GQEIDISTPDAKVRVLVIPTNEELTIARDTKEICE 397 >dbj|BAB71961.2| acetate kinase [Methanosarcina mazei] Length = 408 Score = 462 bits (1190), Expect = e-129, Method: Compositional matrix adjust. Identities = 218/398 (54%), Positives = 300/398 (75%), Gaps = 1/398 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M +LVIN+GSSSLKYQL +MT+ES LA GL ERIG+D+SI+ K +++ + +++ H Sbjct: 1 MKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQTDLPNH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 A+ +V+ LTD E GVI S+DEINAVGHRVVHGGE F +SAL++ + I+ F+LA Sbjct: 61 KIALEEVVKALTDSEFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIKDCFELA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP +MGIS+ + MPGVP V VFDTAFH T+P AYMYA+P LY KY +R+YG H Sbjct: 121 PLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHTIPPYAYMYALPYELYEKYGIRKYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V+K AA L +P +D+K+ITCH+GNG+S+TAV GG+SI+T+MG TPLEG+ MGT Sbjct: 181 GTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLEGVAMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 R G +DPA+VP++M KE LS E+++++NK SG+L +S +S+D RD+ + A KG + L Sbjct: 241 RCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKGNQKAEL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A +++ Y+++K IG Y A +NGVD IVFTAG+GEN+A +R ++L +L +GI++D E NK Sbjct: 301 ALEIFAYKIKKVIGEYIAVLNGVDAIVFTAGIGENSATIRKRILADLDGIGIKIDEEKNK 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 +R G IST ++ V+VLV+PTNEEL IARDT I + Sbjct: 361 IR-GQEIDISTPDATVRVLVIPTNEELTIARDTKEICE 397 >sp|P38502|ACKA_METTE Acetate kinase (Acetokinase) pir||B49338 acetate kinase (EC 2.7.2.1) - Methanosarcina thermophila pdb|1G99|A Chain A, An Ancient Enzyme: Acetate Kinase From Methanosarcina Thermophila pdb|1G99|B Chain B, An Ancient Enzyme: Acetate Kinase From Methanosarcina Thermophila gb|AAA72042.1| acetate kinase Length = 408 Score = 462 bits (1190), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/398 (55%), Positives = 299/398 (75%), Gaps = 1/398 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M +LVIN+GSSSLKYQL +MT+ES LA GL ERIG+D+SI+ K +++ +++++ H Sbjct: 1 MKVLVINAGSSSLKYQLIDMTNESALAVGLCERIGIDNSIITQKKFDGKKLEKLTDLPTH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 A+ +V+ LTD E GVI + EINAVGHRVVHGGE F SAL D + I+ F+LA Sbjct: 61 KDALEEVVKALTDDEFGVIKDMGEINAVGHRVVHGGEKFTTSALYDEGVEKAIKDCFELA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP +MGISA MPG P V+VFDTAFHQTMP AYMYA+P LY K+ VR+YG H Sbjct: 121 PLHNPPNMMGISACAEIMPGTPMVIVFDTAFHQTMPPYAYMYALPYDLYEKHGVRKYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V++ AA L +P E+ KIITCH+GNG+S+TAV+GG+S++TSMG TPLEGL MGT Sbjct: 181 GTSHKYVAERAALMLGKPAEETKIITCHLGNGSSITAVEGGKSVETSMGFTPLEGLAMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 R G +DPAIVP++M KE L+ E+++++NK SG+L +SG+S+D RD+ + A KG + L Sbjct: 241 RCGSIDPAIVPFLMEKEGLTTREIDTLMNKKSGVLGVSGLSNDFRDLDEAASKGNRKAEL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A +++ Y+++K+IG Y+A +NG D +VFTAG+GEN+A +R ++L L +GI++D E NK Sbjct: 301 ALEIFAYKVKKFIGEYSAVLNGADAVVFTAGIGENSASIRKRILTGLDGIGIKIDDEKNK 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 +R G IST ++KV+V V+PTNEEL IAR+T IV+ Sbjct: 361 IR-GQEIDISTPDAKVRVFVIPTNEELAIARETKEIVE 397 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 555,631,500 Number of extensions: 22507942 Number of successful extensions: 60695 Number of sequences better than 1.0e-05: 270 Number of HSP's gapped: 60043 Number of HSP's successfully gapped: 271 Length of query: 402 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 274 Effective length of database: 409,704,906 Effective search space: 112259144244 Effective search space used: 112259144244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)