BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0016.p (458 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_979332.1| glutamate 5-kinase [Bacillus cereus ATCC 10987]... 281 2e-74 ref|NP_691973.1| gamma-glutamyl kinase [Oceanobacillus iheyensis... 280 4e-74 ref|NP_389195.1| gamma-glutamyl kinase [Bacillus subtilis] >gi|3... 278 3e-73 ref|NP_656860.1| aakinase, Amino acid kinase family [Bacillus an... 277 3e-73 ref|NP_832721.1| Glutamate 5-kinase [Bacillus cereus ATCC 14579]... 276 1e-72 >ref|NP_979332.1| glutamate 5-kinase [Bacillus cereus ATCC 10987] gb|AAS41940.1| glutamate 5-kinase [Bacillus cereus ATCC 10987] Length = 367 Score = 281 bits (720), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 148/317 (46%), Positives = 202/317 (63%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSL GG++++ ++ A +A L+++G EVLL+TSGAVAAGF A+GYP+RP Sbjct: 7 VVKIGSSSLADSHGGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNR 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++S++EL FGDND LSALV+ L+ A L++ TD++GLY +P+K+ A + Sbjct: 127 SALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKK 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + E+T EI ++ M+SKIDAAK A GV VF+G Sbjct: 187 YYFLPEVTEEIASLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEKFVDVLK 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY + + + KQW+ S G + +D GA A++ G SLLP GV + G Sbjct: 247 GKGDGTYVGNAPQKEIKMNKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSG 306 Query: 300 SFHSGDVVEVLGPDARL 316 F GDVVEV+ R+ Sbjct: 307 FFKVGDVVEVMTQQGRV 323 >ref|NP_691973.1| gamma-glutamyl kinase [Oceanobacillus iheyensis HTE831] sp|Q8CUQ5|PROB_OCEIH Glutamate 5-kinase (Gamma-glutamyl kinase) (GK) dbj|BAC13008.1| gamma-glutamyl kinase [Oceanobacillus iheyensis HTE831] Length = 372 Score = 280 bits (717), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 142/310 (45%), Positives = 199/310 (64%), Gaps = 1/310 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 V+KIGSSSLT +G ++ K + +A L + EV+LV+SGAVAAGFR +GY +RP Sbjct: 7 VIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLGYSSRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 L KQA+AA+GQ +L+ Y + F EY I +AQ+LLTR+DF ++ NA T+ ELL + Sbjct: 67 TLKGKQASAAIGQGLLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTMLELLER 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 G +PI NENDTV+VDEL FGDND LSALV+ + A+ L++LTD+DG+Y P K+ A R Sbjct: 127 GVIPIINENDTVAVDELTFGDNDMLSALVSGSIHAAQLIILTDIDGIYDKHPGKYASAMR 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y+ ++ IT ++ M+SK+ AAK+A GVPVF+G+ L Sbjct: 187 YDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSKLLEIVN 246 Query: 241 GTGKGTYFDTTLS-SLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G+GTY + + +P++KQW+ ST G +++D+GA +AL+H G SLL GV +G Sbjct: 247 GHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAGVINTQG 306 Query: 300 SFHSGDVVEV 309 F GDVVEV Sbjct: 307 DFEDGDVVEV 316 >ref|NP_389195.1| gamma-glutamyl kinase [Bacillus subtilis] sp|P39820|PROB_BACSU Glutamate 5-kinase 1 (Gamma-glutamyl kinase 1) (GK 1) pir||D69682 gamma-glutamyl kinase proB - Bacillus subtilis emb|CAA05591.1| gamma-glutamyl kinase [Bacillus subtilis] emb|CAB13169.1| glutamate 5-kinase [Bacillus subtilis subsp. subtilis str. 168] Length = 365 Score = 278 bits (710), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 146/313 (46%), Positives = 193/313 (61%), Gaps = 1/313 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSLT +G ++ + I+ L+K G E++L+TSG VA GF ++GYP+RP Sbjct: 7 VVKIGSSSLTNSKGSIDEAKIREHVQAISVLKKAGHEMILITSGPVARGFSSLGYPSRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQ Y F +Y + QILLTR DF R NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQTLLMQQYMNQFKQYSLTPGQILLTRNDFSKRERYRNAYATIMELLER 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 G +PI NEND+ SV+EL FGDND LSALV+ L+ A L++LTD++GLY +P ++P A R Sbjct: 127 GVIPIINENDSTSVEELTFGDNDMLSALVSGLIHADQLMILTDINGLYDANPNENPEAKR 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 ++ + EITPE+ M+SK+ A + A GV VF+G + L Sbjct: 187 FDYLPEITPELLGYAGSAGSKVGTGGMKSKLLATQTALSLGVKVFIGTGSGEQKLADILD 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY D LSS+ +QW+ F S G + +D GA EA++H G SLLP GV + G Sbjct: 247 GRGDGTYIGDKELSSVNNTRQWIQFHSPISGEIIIDAGAEEAMIHNGSSLLPAGVVGVNG 306 Query: 300 SFHSGDVVEVLGP 312 SF G VVEV GP Sbjct: 307 SFPKGAVVEVRGP 319 >ref|NP_656860.1| aakinase, Amino acid kinase family [Bacillus anthracis A2012] ref|NP_845326.1| glutamate 5-kinase [Bacillus anthracis str. Ames] sp|Q81P26|PROB_BACAA Glutamate 5-kinase (Gamma-glutamyl kinase) (GK) gb|AAP26812.1| glutamate 5-kinase [Bacillus anthracis str. Ames] Length = 367 Score = 277 bits (709), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 145/317 (45%), Positives = 201/317 (63%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSL G++++ ++ A +A L+++G EVLL+TSGAVAAGF A+GYP+RP Sbjct: 7 VVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNR 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++S++EL FGDND LSALV+ L+ A L++ TD++GLY +P+K+ A + Sbjct: 127 SALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKK 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + E+T EI ++ M+SK+DAAK A GV VF+G Sbjct: 187 YYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLK 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY + + + KQW+ S G + +D GA A++ G SLLP GV + G Sbjct: 247 GKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSG 306 Query: 300 SFHSGDVVEVLGPDARL 316 F G+VVEV+ R+ Sbjct: 307 FFQVGEVVEVMTQQGRV 323 >ref|NP_832721.1| Glutamate 5-kinase [Bacillus cereus ATCC 14579] sp|Q81C10|PROB_BACCR Glutamate 5-kinase (Gamma-glutamyl kinase) (GK) gb|AAP09922.1| Glutamate 5-kinase [Bacillus cereus ATCC 14579] Length = 367 Score = 276 bits (705), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 147/317 (46%), Positives = 200/317 (63%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSL GG++ + ++ A +A L++DG EV+L+TSGAVAAGF A+GYP+RP Sbjct: 7 VVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALGYPSRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNR 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++S++EL FGDND LSALV+ L+ A L++ TD++GLY +P+K+ A + Sbjct: 127 SALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNADAKK 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + E+T EI ++ M+SKIDAAK A GV VF+G Sbjct: 187 YYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEKFVNVLK 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY + + + KQW+ S G + VD GA A++ G SLLP GV + Sbjct: 247 GKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAGVTNVSR 306 Query: 300 SFHSGDVVEVLGPDARL 316 F G+VVEV+ R+ Sbjct: 307 FFQVGEVVEVVTQQGRV 323 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 619,501,794 Number of extensions: 23662020 Number of successful extensions: 57136 Number of sequences better than 1.0e-05: 596 Number of HSP's gapped: 56578 Number of HSP's successfully gapped: 596 Length of query: 458 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 329 Effective length of database: 406,989,807 Effective search space: 133899646503 Effective search space used: 133899646503 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)