BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0010.p (208 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q9LCR5|LEUD_PAEPO 3-isopropylmalate dehydratase small subunit... 303 2e-81 ref|NP_284179.1| putative 3-isopropylmalate dehydratase small su... 259 3e-68 ref|NP_274068.1| 3-isopropylmalate dehydratase, small subunit [N... 257 1e-67 ref|NP_870631.1| 3-isopropylmalate dehydratase small subunit [Pi... 251 1e-65 ref|NP_520110.1| PROBABLE 3-ISOPROPYLMALATE DEHYDRATASE (SMALL S... 249 3e-65 >sp|Q9LCR5|LEUD_PAEPO 3-isopropylmalate dehydratase small subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI) dbj|BAA90647.1| LeuD [Paenibacillus polymyxa] Length = 178 Score = 303 bits (776), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 142/175 (81%), Positives = 155/175 (88%) Query: 31 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEMNKPRYKGASILISRANFGCGSP 90 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGN N FE NKPRY GAS+LISRANFGCGS Sbjct: 1 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGNINPEFEPNKPRYAGASVLISRANFGCGSS 60 Query: 91 REHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIKLSESQVDELFKRTAEHDGYKLT 150 REHAPWAIMDYGFR VIAPS+ADIFYNNCFKNGILPIKLSE QV++LF+RTA+HD Y+L Sbjct: 61 REHAPWAIMDYGFRCVIAPSFADIFYNNCFKNGILPIKLSEEQVEDLFQRTAKHDNYQLN 120 Query: 151 VDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLTLQHADEIAAYEERHAAK 205 VDL ++DD+GL I F+LDEHRRQFLLQGLDDIGLTLQH EI AYE++ AAK Sbjct: 121 VDLNEKTITDDYGLRIEFDLDEHRRQFLLQGLDDIGLTLQHESEILAYEQKRAAK 175 >ref|NP_284179.1| putative 3-isopropylmalate dehydratase small subunit [Neisseria meningitidis Z2491] sp|Q9JU81|LEUD_NEIMA 3-isopropylmalate dehydratase small subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI) pir||C81836 probable 3-isopropylmalate dehydratase (EC 4.2.1.33) small chain NMA1452 [imported] - Neisseria meningitidis (strain Z2491 serogroup A) emb|CAB84688.1| putative 3-isopropylmalate dehydratase small subunit [Neisseria meningitidis Z2491] Length = 213 Score = 259 bits (661), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 123/202 (60%), Positives = 154/202 (76%), Gaps = 9/202 (4%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRF---DEAGNDNAA 65 M+AF K+T IVAP+DR NVDTDAIIPKQFLK I+R+GFG F EWR+ E G DN+ Sbjct: 1 MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFDEWRYLDHGEPGMDNSK 60 Query: 66 ------FEMNKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNC 119 F +N+PRY+GA IL++R NFGCGS REHAPWA+ DYGFR VIAPS+ADIF+NNC Sbjct: 61 RPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDYGFRAVIAPSFADIFFNNC 120 Query: 120 FKNGILPIKLSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLL 179 +KNG+LPI L+E QVD LFK ++GY+L++DL L+ G + TF++ EHR+ LL Sbjct: 121 YKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLTTPSGETFTFDITEHRKHCLL 180 Query: 180 QGLDDIGLTLQHADEIAAYEER 201 GLD+IGLTLQHADEI A+EE+ Sbjct: 181 NGLDEIGLTLQHADEIHAFEEK 202 >ref|NP_274068.1| 3-isopropylmalate dehydratase, small subunit [Neisseria meningitidis MC58] sp|Q9JZI6|LEUD_NEIMB 3-isopropylmalate dehydratase small subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI) pir||E81128 3-isopropylmalate dehydratase, small chain NMB1034 [imported] - Neisseria meningitidis (strain MC58 serogroup B) gb|AAF41433.1| 3-isopropylmalate dehydratase, small subunit [Neisseria meningitidis MC58] Length = 213 Score = 257 bits (656), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 122/202 (60%), Positives = 154/202 (76%), Gaps = 9/202 (4%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRF---DEAGNDNAA 65 M+AF K+T IVAP+DR NVDTDAIIPKQFLK I+R+GFG F EWR+ E G DN+ Sbjct: 1 MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFDEWRYLDHGEPGMDNSK 60 Query: 66 ------FEMNKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNC 119 F +N+PRY+GA IL++R NFGCGS REHAPWA+ DYGFR VIAPS+ADIF+NNC Sbjct: 61 RPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDYGFRAVIAPSFADIFFNNC 120 Query: 120 FKNGILPIKLSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLL 179 +KNG+LPI L+E +VD LFK ++GY+L++DL L+ G + TF++ EHR+ LL Sbjct: 121 YKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLTTPSGETFTFDITEHRKHCLL 180 Query: 180 QGLDDIGLTLQHADEIAAYEER 201 GLD+IGLTLQHADEI A+EE+ Sbjct: 181 NGLDEIGLTLQHADEIHAFEEK 202 >ref|NP_870631.1| 3-isopropylmalate dehydratase small subunit [Pirellula sp. 1] emb|CAD77708.1| 3-isopropylmalate dehydratase small subunit [Pirellula sp.] Length = 196 Score = 251 bits (640), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 120/193 (62%), Positives = 147/193 (76%), Gaps = 1/193 (0%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAG-NDNAAFE 67 M+ F G+VA +DR NVDTD IIPKQFLKRIERTGFGQFLF++WRF E G +N FE Sbjct: 1 MQNFTVHQGVVATLDRANVDTDQIIPKQFLKRIERTGFGQFLFFDWRFLEDGETENPDFE 60 Query: 68 MNKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPI 127 +N+ KGASIL++R NFG GS REHA WA+ DYGFR VIAPS+ADIF+NNCFKNG+LPI Sbjct: 61 LNRINVKGASILLTRQNFGSGSSREHAVWALDDYGFRAVIAPSFADIFFNNCFKNGVLPI 120 Query: 128 KLSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGL 187 LSE V+ELF+R + + Y+LTVDLEN +++D G +FE+D RR +L GLDDI Sbjct: 121 ALSEEDVEELFQRAEKGNPYQLTVDLENQVITDGQGFERSFEVDASRRHNMLHGLDDIAQ 180 Query: 188 TLQHADEIAAYEE 200 TL H D+I A+EE Sbjct: 181 TLLHEDKITAFEE 193 >ref|NP_520110.1| PROBABLE 3-ISOPROPYLMALATE DEHYDRATASE (SMALL SUBUNIT) PROTEIN [Ralstonia solanacearum] sp|Q8XXX4|LEUD_RALSO 3-isopropylmalate dehydratase small subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI) emb|CAD15691.1| PROBABLE 3-ISOPROPYLMALATE DEHYDRATASE (SMALL SUBUNIT) PROTEIN [Ralstonia solanacearum] Length = 216 Score = 249 bits (636), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 121/207 (58%), Positives = 151/207 (72%), Gaps = 9/207 (4%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFD---EAGNDNAA 65 ME F TG+VAP+DR NVDTDAIIPKQFLK I+RTGFG LF EWR+ E G DN+ Sbjct: 1 MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSK 60 Query: 66 ------FEMNKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNC 119 F +N+PRY+GAS+L++R NFGCGS REHAPWA+ YGFR +IAPS+ADIF+NNC Sbjct: 61 RPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC 120 Query: 120 FKNGILPIKLSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLL 179 FKNG+LPI L+ESQVD LF T GY+LTVDL+ ++ G + F++ R+ LL Sbjct: 121 FKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVVTPGGTAYPFDVTAFRKYCLL 180 Query: 180 QGLDDIGLTLQHADEIAAYEERHAAKL 206 G DDIGLTL++AD+I AYE + AK+ Sbjct: 181 NGFDDIGLTLRYADQIKAYEAQRLAKM 207 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 311,264,958 Number of extensions: 13154508 Number of successful extensions: 29762 Number of sequences better than 1.0e-05: 652 Number of HSP's gapped: 29189 Number of HSP's successfully gapped: 652 Length of query: 208 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 88 Effective length of database: 431,425,698 Effective search space: 37965461424 Effective search space used: 37965461424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)