BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0007.p (479 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA90649.1| CwlV [Paenibacillus polymyxa] 451 e-125 dbj|BAA90648.1| CwlU [Paenibacillus polymyxa] 322 2e-86 pdb|1JWQ|A Chain A, Structure Of The Catalytic Domain Of Cwlv, N... 226 8e-58 ref|ZP_00157744.1| hypothetical protein Avar555501 [Anabaena var... 131 3e-29 ref|NP_484136.1| N-acetylmuramoyl-L-alanine amidase [Nostoc sp. ... 130 7e-29 >dbj|BAA90649.1| CwlV [Paenibacillus polymyxa] Length = 499 Score = 451 bits (1161), Expect = e-125, Method: Compositional matrix adjust. Identities = 248/501 (49%), Positives = 318/501 (63%), Gaps = 29/501 (5%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKKF F+ PG GHAA +I+LD ++L+ + + S MVPLR+I+ Sbjct: 1 MKKFGFLLLLFVFMWAVPGYGHAASSGTQIYLDDQQLSVPSGAKAQVIGGSTMVPLRVIS 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 ENLGY V W Q+++T+TIE+ +I++I+ A+V+ + L +PLLRS+ LVP+RF Sbjct: 61 ENLGYDVTWKQQARTITIEKDSTSIRMIIGSKTATVNGSDISLDASPLLRSNYALVPLRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNLITPEIPDSTSNTGE----SSGDGGT---SVAVPPVDTTAN 173 IGEQ GL V WD+++K V L T S S GE SG+ T SV P + ++ Sbjct: 121 IGEQMGLDVKWDSSSKSVKLYTRS---SGSGNGEFTPPKSGNNSTGTGSVTAPTNNASSG 177 Query: 174 LTMVNGISFSENRLTIAMDGNSQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDLNG 233 +T V G+SFS+NRLTI +P +TGPDR+V+DLP F+D FG Q L +LNG Sbjct: 178 VTQVQGVSFSQNRLTITTASGVKPKAFTMTGPDRVVVDLPATAFADNFGDQQKLDSNLNG 237 Query: 234 SLAVTDYPDVSGVRYSLYSTNPYTVRFVIDLNNAKNYSVNVSGDVSKLVVVDL------- 286 SL + + DVSG+RY+L+ P TVR VIDL +A NY+ D S V+VDL Sbjct: 238 SLDIENEADVSGIRYALFQKEPSTVRVVIDLKHAMNYTA--YNDGSNRVIVDLASKDSVN 295 Query: 287 --------NAASTDDPATLPGN-NGRKLVVLDAGHGAKDSGAVGVTGK-YEKNFNXXXXX 336 + + DD T P N NG+K+VV+DAGHGAKDSGAVG++ K YEK FN Sbjct: 296 TTPDATLPDGSQPDDTQTSPVNSNGKKVVVIDAGHGAKDSGAVGISRKNYEKTFNLAMAL 355 Query: 337 XXXXXXXQENKVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXXXETY 396 Q K++VVLTRSDDTFLELK+R +A NLKA++F+S+HAN ETY Sbjct: 356 KVESILKQNPKLEVVLTRSDDTFLELKQRVKVAENLKANVFVSIHANSSGSSASNGTETY 415 Query: 397 YQRAESKALANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALL 456 YQR+ SKA ANVMHKY ATGL+DRG+RYGNFHVIRETTMPAVLLEVGYLSN +EA L Sbjct: 416 YQRSASKAFANVMHKYFAPATGLTDRGIRYGNFHVIRETTMPAVLLEVGYLSNAKEEATL 475 Query: 457 FSEDLQNKVAAGMVAGIKEYL 477 F ED QN+VA G+ GI EYL Sbjct: 476 FDEDFQNRVAQGIADGITEYL 496 >dbj|BAA90648.1| CwlU [Paenibacillus polymyxa] Length = 524 Score = 322 bits (824), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 192/518 (37%), Positives = 274/518 (52%), Gaps = 46/518 (8%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKK+ ++ P H ++FLDGK + A D E VN +MVPLR++ Sbjct: 1 MKKYGWVILAAILIWLWPSSPHVNAAGAELFLDGKRVEAPADAKPEMVNGKVMVPLRVVG 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 E LGYQ W+ ++ ++I++ + + V +T+A V+ KTV L P+LR +T++P+RF Sbjct: 61 EQLGYQFKWEPQAYKISIQKNSTDMSMYVGRTSADVNGKTVSLDAPPVLRGSSTMIPLRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNL---ITPEIPD-----------------STSNTGESS---- 156 +GEQ GLKV W+N K VNL I + D ST+ T ++ Sbjct: 121 VGEQMGLKVDWNNQNKSVNLSQKIATQPADKQSQSQQPSQTSTQSPKSTNKTSTATATTI 180 Query: 157 ----GDGGTSVAVPPV-------DTTANLTMVNGISFSENRLTIAMDGNSQPVVSKVTGP 205 D T V VP TTAN +V ISF L I+++ + P VSK+TGP Sbjct: 181 NPVEADKHTEV-VPSTSKEQNVEQTTANAVLVQNISFESETLKISLNKDLTPKVSKMTGP 239 Query: 206 DRIVIDLPNATFSDVFGTGQVLSPDLNGSLAVTDYPDVSGVRYSLYSTNPYTVRFVIDLN 265 DRIV+DLP A+ S + D +L ++D DV +R++ R VI LN Sbjct: 240 DRIVVDLPEASLSADLLQSFPVRKDGTRALTISDSEDVQEIRFAPADGTKGGARIVIALN 299 Query: 266 NAKNY--SVNVSGDVSKLVVVDLNAASTDDPATLPGN-NGRKLVVLDAGHGAKDSGAVGV 322 ++Y S N GD++ + L S + P GR++VV+D GHG KD GA V Sbjct: 300 QTRDYDLSTNGVGDIT----LQLRERSVNPEIVAPTYVEGRRIVVIDPGHGGKDPGAGSV 355 Query: 323 TGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDTFLELKERAGMANNLKADLFISVHA 382 TG++EK F + + +VLTR+ DT+ L ER +ANN +A +F+S+H Sbjct: 356 TGRHEKEFTLAVALKVQQLAQNDPDIQIVLTRNGDTYPTLDERPQLANNQQASVFVSIHG 415 Query: 383 NXXXXX---XXXXXETYYQRAESKALANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPA 439 N ETYY R ES +LA +MHK+LV AT D G++ N V R++ MPA Sbjct: 416 NSMPTSNNGKANGSETYYARQESLSLATIMHKHLVAATEFKDNGIKVANHVVTRKSQMPA 475 Query: 440 VLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYL 477 VLLE GYLSN +EA +F+E+ Q K+A G+V G+KEYL Sbjct: 476 VLLECGYLSNPSNEAAMFTEETQQKIAEGIVDGLKEYL 513 >pdb|1JWQ|A Chain A, Structure Of The Catalytic Domain Of Cwlv, N-Acetylmuramoyl- L-Alanine Amidase From Bacillus(Paenibacillus) Polymyxa Var.Colistinus Length = 179 Score = 226 bits (577), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 113/175 (64%), Positives = 131/175 (74%), Gaps = 1/175 (0%) Query: 304 KLVVLDAGHGAKDSGAVGVTGK-YEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDTFLEL 362 K+VV+DAGHGAKDSGAVG++ K YEK FN Q K++VVLTRSDDTFLEL Sbjct: 2 KVVVIDAGHGAKDSGAVGISRKNYEKTFNLAMALKVESILKQNPKLEVVLTRSDDTFLEL 61 Query: 363 KERAGMANNLKADLFISVHANXXXXXXXXXXETYYQRAESKALANVMHKYLVQATGLSDR 422 K+R +A NLKA++F+S+HAN ETYYQR+ SKA ANVMHKY ATGL+DR Sbjct: 62 KQRVKVAENLKANVFVSIHANSSGSSASNGTETYYQRSASKAFANVMHKYFAPATGLTDR 121 Query: 423 GVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYL 477 G+RYGNFHVIRETTMPAVLLEVGYLSN +EA LF ED QN+VA G+ GI EYL Sbjct: 122 GIRYGNFHVIRETTMPAVLLEVGYLSNAKEEATLFDEDFQNRVAQGIADGITEYL 176 >ref|ZP_00157744.1| hypothetical protein Avar555501 [Anabaena variabilis ATCC 29413] Length = 627 Score = 131 bits (330), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 71/194 (36%), Positives = 107/194 (55%), Gaps = 4/194 (2%) Query: 287 NAASTDDPATLPGNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQEN 346 N P T P GR +V++D GHG KD GA+G+ G EK+ Q+N Sbjct: 435 NPRPLPQPGTRPIPKGRLVVLIDPGHGGKDPGAIGIGGVREKDI-ILPISQRIAEVLQQN 493 Query: 347 KVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXXX--ETYYQRAESKA 404 V VV+ R+ D F+ L R MA + AD+F+S+HAN ETYY + + Sbjct: 494 GVQVVMARNSDYFVSLPGRVQMAERVGADVFVSIHANSAGAGRPDVSGLETYYYDS-GLS 552 Query: 405 LANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNK 464 LA V+H +++ + DRGVR F+V+R+++MP++L+E GYL+ + D A L + Q + Sbjct: 553 LARVVHSSILRNVNVRDRGVRRARFYVLRKSSMPSILVETGYLTGRDDAAKLRNSAYQRQ 612 Query: 465 VAAGMVAGIKEYLK 478 +A + GI +YLK Sbjct: 613 MADAIARGILQYLK 626 >ref|NP_484136.1| N-acetylmuramoyl-L-alanine amidase [Nostoc sp. PCC 7120] pir||AD1818 N-acetylmuramoyl-L-alanine amidase [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB77616.1| N-acetylmuramoyl-L-alanine amidase [Nostoc sp. PCC 7120] Length = 627 Score = 130 bits (327), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 71/194 (36%), Positives = 103/194 (53%), Gaps = 4/194 (2%) Query: 287 NAASTDDPATLPGNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQEN 346 N P T P GR V +D GHG KD GA+G+ G EK+ Q+N Sbjct: 435 NPRPLPQPGTRPVPKGRLSVFIDPGHGGKDPGAIGIGGVREKDI-ILPISQRIAQVLQQN 493 Query: 347 KVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXX--XXXXXXXXETYYQRAESKA 404 V VV+ R+ D F+ L R MA +AD+F+S+HAN ETYY + Sbjct: 494 GVQVVMARNSDFFVSLPGRVQMAERARADVFVSIHANAIGGNRADVNGLETYYYDS-GLG 552 Query: 405 LANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNK 464 LA +H+ ++Q + DRGVR F+V+R+++MP++L+E GYL+ + D L S Q + Sbjct: 553 LARAVHRSILQNVNVRDRGVRRARFYVLRKSSMPSILVETGYLTGRDDNGKLQSPAYQRQ 612 Query: 465 VAAGMVAGIKEYLK 478 +A + GI +YLK Sbjct: 613 MADAIARGILQYLK 626 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 661,301,454 Number of extensions: 27124310 Number of successful extensions: 56425 Number of sequences better than 1.0e-05: 329 Number of HSP's gapped: 56028 Number of HSP's successfully gapped: 335 Length of query: 479 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 350 Effective length of database: 406,989,807 Effective search space: 142446432450 Effective search space used: 142446432450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)