BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0008.p (328 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P48790|XYLA_CLOSR Xylosidase/arabinosidase [Includes: Beta-xy... 467 e-130 pir||JQ1936 xylan 1,4-beta-xylosidase (EC 3.2.1.37) [validated] ... 462 e-129 ref|NP_809282.1| endo-1,4-beta-xylanase D precursor [Bacteroides... 72 1e-11 gb|EAA78496.1| hypothetical protein FG11468.1 [Gibberella zeae P... 68 2e-10 ref|ZP_00060110.1| COG3507: Beta-xylosidase [Clostridium thermoc... 64 6e-09 >sp|P48790|XYLA_CLOSR Xylosidase/arabinosidase [Includes: Beta-xylosidase (1,4-beta-D-xylan xylohydrolase) (Xylan 1,4-beta-xylosidase); Alpha-L-arabinofuranosidase (Arabinosidase)] dbj|BAA02527.1| xylosidase [Clostridium stercorarium] prf||1909151A beta xylosidase Length = 473 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 221/328 (67%), Positives = 261/328 (79%), Gaps = 4/328 (1%) Query: 1 LTEGDTTYLYXXXXCGYGDKSRHGAMATVLGPDMLTIIEESVFVAPSQPYSKXXXXXXXX 60 LTEG+ TYLY C GDKSR GAMATVLGPDMLT++EE V + PS+PYS+ Sbjct: 148 LTEGERTYLYTGF-CPKGDKSRKGAMATVLGPDMLTVVEEPVIIVPSEPYSRGSGFEGHE 206 Query: 61 XXXAPSIRKRGDIYYLIYSSISMHELCYATSKHPTRGFTYQGVIVSNCDLHIDTYKPADQ 120 APSIRK+GD YY IYSS+ MHELCYATSKHPT+GF Y GVIVSNCDLHID+YKPA++ Sbjct: 207 FFEAPSIRKKGDTYYFIYSSVVMHELCYATSKHPTKGFKYGGVIVSNCDLHIDSYKPAEK 266 Query: 121 PMYYGGNNHGSIVEVNGEWYIFYHRHTNGTAFSRQGCIEPITFREDGTIPQVEITTSGPN 180 PMYYGGNNHGSIVE+NGEWYIFYHRHTNGT+FSRQGC+E I EDG+IPQVE+T+ G Sbjct: 267 PMYYGGNNHGSIVEINGEWYIFYHRHTNGTSFSRQGCMEKIKILEDGSIPQVEMTSCGSA 326 Query: 181 GGPLAGRGEYPAYLACHLFTKDQEMYTGGFGPMGAWMDSRFPKITQDGRDGDDEIGYIAN 240 PL GRGEYPAY+AC+LF ++ +YT GAWM+++FPKITQDG+DGD+E GYIAN Sbjct: 327 DEPLPGRGEYPAYIACNLFCGEESVYT---DLTGAWMNNQFPKITQDGKDGDEEPGYIAN 383 Query: 241 MTDSATAGFKYFDCQGVTRVSIKVRGYCHGEFQVKTAWDGPVLASIPVGFTNVWTEYTTD 300 M DSATAGFKYFDC+G+ V IKVRGYC G F+VKT+W+G VL IPV F+N+WTE++ Sbjct: 384 MKDSATAGFKYFDCKGIKSVKIKVRGYCRGVFEVKTSWNGEVLGKIPVEFSNIWTEFSAS 443 Query: 301 VAIPDGVQSLYFTYTGGGGAQLGSFTLA 328 + IPDG+ +LYFTY G G A L SFTL Sbjct: 444 IPIPDGIHALYFTYRGSGSASLKSFTLC 471 >pir||JQ1936 xylan 1,4-beta-xylosidase (EC 3.2.1.37) [validated] - Clostridium stercorarium Length = 473 Score = 462 bits (1188), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/328 (66%), Positives = 259/328 (78%), Gaps = 4/328 (1%) Query: 1 LTEGDTTYLYXXXXCGYGDKSRHGAMATVLGPDMLTIIEESVFVAPSQPYSKXXXXXXXX 60 LTEG+ TYLY C GDKSR GAMATVLGPDMLT++EE V + PS+PYS+ Sbjct: 148 LTEGERTYLYTGF-CPKGDKSRKGAMATVLGPDMLTVVEEPVIIVPSEPYSRGSGFEGHE 206 Query: 61 XXXAPSIRKRGDIYYLIYSSISMHELCYATSKHPTRGFTYQGVIVSNCDLHIDTYKPADQ 120 APSIRK+G+ YY I SS+ MHELCYATSKHPT+GF Y GVIVSNCDLHID+YKPA++ Sbjct: 207 FFEAPSIRKKGETYYFITSSVVMHELCYATSKHPTKGFKYGGVIVSNCDLHIDSYKPAEK 266 Query: 121 PMYYGGNNHGSIVEVNGEWYIFYHRHTNGTAFSRQGCIEPITFREDGTIPQVEITTSGPN 180 PMYYGGNNHGSIVE+NGEWYIFYHRHTNGT+FSRQGC+E I EDG+IPQVE+T+ G Sbjct: 267 PMYYGGNNHGSIVEINGEWYIFYHRHTNGTSFSRQGCMEKIKILEDGSIPQVEMTSCGSA 326 Query: 181 GGPLAGRGEYPAYLACHLFTKDQEMYTGGFGPMGAWMDSRFPKITQDGRDGDDEIGYIAN 240 PL GRGEYPAY+AC LF ++ +YT GAWM+++FPKITQDG+DGD+E GYIAN Sbjct: 327 DEPLPGRGEYPAYIACMLFCGEESVYT---DLTGAWMNNQFPKITQDGKDGDEEPGYIAN 383 Query: 241 MTDSATAGFKYFDCQGVTRVSIKVRGYCHGEFQVKTAWDGPVLASIPVGFTNVWTEYTTD 300 M DSATAGFKYFDC+G+ V IKVRGYC G F+VKT+W+G VL IPV F+N+WTE++ Sbjct: 384 MKDSATAGFKYFDCKGIKSVKIKVRGYCRGVFEVKTSWNGEVLGKIPVEFSNIWTEFSAS 443 Query: 301 VAIPDGVQSLYFTYTGGGGAQLGSFTLA 328 + IPDG+ +LYFTY G G A L SFTL Sbjct: 444 IPIPDGIHALYFTYRGSGSASLKSFTLC 471 >ref|NP_809282.1| endo-1,4-beta-xylanase D precursor [Bacteroides thetaiotaomicron VPI-5482] gb|AAO75476.1| endo-1,4-beta-xylanase D precursor [Bacteroides thetaiotaomicron VPI-5482] Length = 321 Score = 72.4 bits (176), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 40/118 (33%), Positives = 63/118 (53%), Gaps = 17/118 (14%) Query: 64 APSIRKRGDIYYLIYSSISMHELCYATSKHPTRGFTYQGVIVSNCDLHIDTYKPADQPMY 123 AP I K GD YYL Y+S ++CYA S+ T + Y+G++ ++ Sbjct: 216 APWIHKCGDWYYLSYASEFPEKICYAMSRSITGPWEYKGIL--------------NEIAG 261 Query: 124 YGGNNHGSIVEVNGEWYIFYHR---HTNGTAFSRQGCIEPITFREDGTIPQVEITTSG 178 NH +I+E G+WY YH +T G +F R CI+ + + EDGT+ ++++TT G Sbjct: 262 NSNTNHQAIIEFKGDWYFIYHNGSINTAGGSFRRSVCIDRLYYNEDGTMKRIQMTTEG 319 >gb|EAA78496.1| hypothetical protein FG11468.1 [Gibberella zeae PH-1] Length = 340 Score = 68.2 bits (165), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 41/106 (38%), Positives = 53/106 (50%), Gaps = 16/106 (15%) Query: 65 PSIRKRGDIYYLIYSSISMHELCYATSKHPTRGFTYQGVIVSNCDLHIDTYKPADQPMYY 124 P I KR IYYL YS+ + H L YATSK P +TYQG I+ D Sbjct: 245 PWIHKRNKIYYLTYSTGTTHYLVYATSKTPYGPYTYQGRILEPVD--------------- 289 Query: 125 GGNNHGSIVEVNGEWYIFYHR-HTNGTAFSRQGCIEPITFREDGTI 169 G H SIV+ G+W++FYH T+G + RQ + I + G I Sbjct: 290 GWTTHSSIVKYQGQWWLFYHDAKTSGKDYLRQVKAKKIWYDSKGKI 335 >ref|ZP_00060110.1| COG3507: Beta-xylosidase [Clostridium thermocellum ATCC 27405] Length = 679 Score = 63.5 bits (153), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 74/318 (23%), Positives = 121/318 (38%), Gaps = 62/318 (19%) Query: 17 YGDKSRHGAMATVLGPDMLTIIEESVFVAPSQPYSKXXXXXXXXXXXAPSIRKRGDIYYL 76 +G ++ LG DM++ + ++ ++ + + A + K YY Sbjct: 181 FGGNGQNNIRVIKLGNDMISTVGSAMTMSAPRFFE------------AAYMHKYNGKYYF 228 Query: 77 IYSS---ISMHELCYATSKHPTRGFTYQGVIVSNCDLHIDTYKPADQPMYYGGNNHGSIV 133 Y+S ++ Y S PT GF Y+GVI+ +P D Y NNH +IV Sbjct: 229 SYASDFSQGASKIEYMMSDKPTTGFQYKGVILP---------QPPDN---YSNNNHHAIV 276 Query: 134 EVNGEWYIFYHRHTNGT------AFSRQGCIEPITFREDGTIPQVEITTSGPNGGPLAGR 187 E G WY+ YH T + R CI+ + + DGTI QV T G Sbjct: 277 EYKGNWYVVYHNRTVAKQRGLDPVYQRNVCIDQMFYNADGTIKQVVPTVDG--------- 327 Query: 188 GEYPAYLACHLFTKDQEMYTGGFGPMGAWMDSRFPKITQDGRDGDDEIGYIANMTDSATA 247 +TK+ + M T++ +G + +I N Sbjct: 328 --LKQLKYVDPYTKN----------LAVTMHKESGIETEECSEGGRNVAFIENGDWIQVK 375 Query: 248 GFKYFDCQGVTRVSIKVRGYCHGEFQVKTAWDGP---VLASIPVGFTNVWTEYTT---DV 301 G + + G T +V +G ++ D P ++ + V T W ++ T V Sbjct: 376 GVDFGNV-GPTSFEARVASATNGG-NIEIRLDSPTGTLIGTCKVEGTGDWQKWVTKTCSV 433 Query: 302 AIPDGVQSLYFTYTGGGG 319 + GV L+F +TGG G Sbjct: 434 SKVTGVHDLFFRFTGGSG 451 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 537,081,587 Number of extensions: 23726768 Number of successful extensions: 35001 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 34982 Number of HSP's successfully gapped: 23 Length of query: 328 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 202 Effective length of database: 415,135,104 Effective search space: 83857291008 Effective search space used: 83857291008 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)