BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0004.p (291 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAK18166.1| unknown [environmental sequence] 220 3e-56 gb|EAJ76634.1| unknown [environmental sequence] 219 8e-56 gb|EAK26316.1| unknown [environmental sequence] 218 2e-55 ref|ZP_00031742.1| COG3618: Predicted metal-dependent hydrolase ... 206 7e-52 gb|EAJ90489.1| unknown [environmental sequence] 199 7e-50 >gb|EAK18166.1| unknown [environmental sequence] Length = 274 Score = 220 bits (561), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 103/278 (37%), Positives = 165/278 (59%), Gaps = 4/278 (1%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 MY+D+HQH+WK+ R DY W+TP++ VL++D+ P DLEP + + I++T++VQAA TV ET Sbjct: 1 MYVDSHQHFWKLSRGDYSWMTPDMKVLYKDFFPEDLEPLIKQKNITKTVIVQAADTVAET 60 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 E+ L L+ + E IAGV S K+ + +L + G R MI +++D +L Sbjct: 61 EFTLSLASKFEFIAGVVGWVDLD--SNNAKDDIDKLSESKYLKGFRPMIHDIQDDTWMLN 118 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQLEPWR 191 IE + YL +++L D LV + L LI+ +++ L VIDH+AKP I +++ W+ Sbjct: 119 DHLIENIKYLNEKNLTFDALVRPNHLQHLIQFVKKYDFLPIVIDHIAKPVIIKSEIDRWK 178 Query: 192 SRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPVCLM 251 M +++K ++CK SG++TE P +T+E Y+ +L +FG +++M+GSDWPV M Sbjct: 179 QNMTELSKASNVFCKFSGILTEVGP-DYTKEQLDPYIDFILNIFGSDKLMWGSDWPVLTM 237 Query: 252 AATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 A Y ++ + S +E +F NA FY L Sbjct: 238 ADNYSNWFDLAMNYCNS-FSEDEKNKIFSQNATQFYSL 274 >gb|EAJ76634.1| unknown [environmental sequence] Length = 278 Score = 219 bits (557), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 112/278 (40%), Positives = 164/278 (58%), Gaps = 3/278 (1%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 M ID+H H+WK D+Y WI+ + L RDYLP+DL H I+ ++ VQA T++ET Sbjct: 1 MNIDSHHHFWKYTADEYPWISTGMERLARDYLPADLIKATQPHDITGSVAVQARQTIQET 60 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 E++L L+ I GV SP+ E L + + KF G+R ++Q+ DP LL Sbjct: 61 EWLLELAANYSFIRGVVGWVDLR--SPSVSEDLEKFSENDKFVGVRHVVQDEPDPQFLLG 118 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQLEPWR 191 ++ L YL +L D+LV QLP E+++ +P V+DH+AKP I + W+ Sbjct: 119 NDFVRGLRYLPAYNLTYDILVFPHQLPAAYELVKMLPDQPFVLDHMAKPDIEHHAISEWK 178 Query: 192 SRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPVCLM 251 +A+H + CKLSG+VTEA+ + W Q DFT Y+ LE FGPER+++GSDWPVCL+ Sbjct: 179 RHFNALAEHENVVCKLSGLVTEANWKVWQQHDFTPYLDVALEAFGPERLLYGSDWPVCLL 238 Query: 252 AATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 +A Y EV+ + ++L R+S E + + G NA FY L Sbjct: 239 SAEYAEVL-CIANSLIDRVSLHEAKAIRGTNATQFYGL 275 >gb|EAK26316.1| unknown [environmental sequence] Length = 274 Score = 218 bits (554), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 102/278 (36%), Positives = 164/278 (58%), Gaps = 4/278 (1%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 MY+D+HQH+WK+ R DY W+TP++ VL++D+ P DLEP + + I++T++VQAA TV ET Sbjct: 1 MYVDSHQHFWKLSRGDYSWMTPDMKVLYKDFFPEDLEPLIKQKNITKTVIVQAADTVAET 60 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 E+ L L+ + E IAGV S K+ + +L ++ G R MI +++D +L Sbjct: 61 EFTLSLASKYEFIAGVVGWVDLD--SNNAKDDIDKLCENKFLKGFRPMIHDIQDDAWMLN 118 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQLEPWR 191 E ++YL ++L D LV + L LI+ +++ L VIDH+AKP I +++ W+ Sbjct: 119 DHLKENINYLNTKNLTFDALVRPNHLQHLIQFVKKYDFLPIVIDHIAKPVITKSEIDQWK 178 Query: 192 SRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPVCLM 251 M +++ +YCK SG++TE P +T+E Y+ +L +FG +++M+GSDWPV M Sbjct: 179 KDMTELSNASNVYCKFSGILTEVGP-DYTKEQLYPYIDFILNIFGSDKLMWGSDWPVLTM 237 Query: 252 AATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 A Y ++ + S +E +F NA FY L Sbjct: 238 ADNYSNWFDLAMNYCNS-FSEDEKNKIFSQNATQFYSL 274 >ref|ZP_00031742.1| COG3618: Predicted metal-dependent hydrolase of the TIM-barrel fold [Burkholderia fungorum] Length = 318 Score = 206 bits (523), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 117/280 (41%), Positives = 159/280 (56%), Gaps = 6/280 (2%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 M+IDAHQHYW R DY W+TPE+ +L+R + P+DL+P R GI RT+VVQAAPT++ET Sbjct: 43 MHIDAHQHYWDPARGDYEWLTPELKILYRTFGPADLKPLRERAGIERTVVVQAAPTIDET 102 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 Y+L ++ SIAGV PT + L KF G+R M+Q++ D + Sbjct: 103 RYLLDIARHEPSIAGVVGWVPLLL--PTAPAVIEALAHEPKFKGVRPMLQDLPDDTWIAN 160 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQ--LEP 189 A+ L DL D L+ A R P LR V+DH AKPPI G+ + Sbjct: 161 PDLAPAIEALIAHDLAFDALIYARHAEPFETFATRFPALRIVVDHGAKPPIRYGRAGYQS 220 Query: 190 WRSRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPVC 249 W + ++A+ P ++CKLSG+ TEA P GWT+ YV +L+ FGP R+M+GSDWPV Sbjct: 221 WADAITRLAQLPHVHCKLSGLATEASP-GWTEATLQPYVDHLLKSFGPARLMWGSDWPVL 279 Query: 250 LMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 + Y + + + L LS E E VFG NAA FY+L Sbjct: 280 DLNGDY-LLWHSVANTLLSSLSDGEREAVFGGNAAAFYRL 318 >gb|EAJ90489.1| unknown [environmental sequence] Length = 279 Score = 199 bits (506), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 111/281 (39%), Positives = 155/281 (55%), Gaps = 7/281 (2%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 M IDAHQH+W R DYGW+ + +L+R YL DLEP+L + GI +TI+VQAAP++EET Sbjct: 1 MKIDAHQHFWNPARGDYGWMPEDNKILYRSYLQHDLEPYLKKLGIDKTILVQAAPSIEET 60 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 EY+LG++ + +AGV A T QL +L H KF G+R M+Q++ D +L Sbjct: 61 EYLLGIADSSTVVAGVVGWVNFEKA--TDYSQLERLSKHPKFVGVRPMVQDIPDETWVLR 118 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPI---ASGQLE 188 A + + + DL D L L + L + +R VIDH KP I + Sbjct: 119 DDIQWAFAAIKEMDLTFDALGFPKHLENFRKCLLKHKDMRTVIDHFMKPRIENHSQDNFN 178 Query: 189 PWRSRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPV 248 W ++ +A+ Y K SG++TEA + WT +D YV +L FGP R+++GSDWPV Sbjct: 179 FWADGISALAEDTSAYIKFSGLITEA-SEDWTIDDLRPYVEHILMKFGPNRILWGSDWPV 237 Query: 249 CLMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 C + A YD S L LS++ VFG NA YKL Sbjct: 238 CRLRAEYD-AWHGTASELTAHLSSDNKANVFGKNAIKAYKL 277 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 433,804,319 Number of extensions: 17750496 Number of successful extensions: 35198 Number of sequences better than 1.0e-05: 134 Number of HSP's gapped: 35109 Number of HSP's successfully gapped: 134 Length of query: 291 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 167 Effective length of database: 420,565,302 Effective search space: 70234405434 Effective search space used: 70234405434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)