BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0003.p (319 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAP21783.1| L-galactose dehydrogenase [Malus x domestica] 278 9e-74 ref|NP_195093.1| L-galactose dehydrogenase (L-GalDH) [Arabidopsi... 276 4e-73 gb|AAO18639.1| galactose dehydrogenase [Actinidia deliciosa] 270 3e-71 ref|NP_812525.1| putative oxidoreductase [Bacteroides thetaiotao... 259 4e-68 gb|EAD84961.1| unknown [environmental sequence] 251 1e-65 >gb|AAP21783.1| L-galactose dehydrogenase [Malus x domestica] Length = 324 Score = 278 bits (712), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 139/313 (44%), Positives = 203/313 (64%), Gaps = 10/313 (3%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 ++ R+LGNTGL+ V DEAI +V A GIN D SPYYG Sbjct: 9 VELRELGNTGLKLSCVGFGASPLGNVFGPVSDDEAIGSVREAFRRGINFFDTSPYYGGTL 68 Query: 61 AETVLGEALQT--VPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILL 118 +E VLG+ L+ VPR +Y+++TK GRY +G FDFS +R+ +S +ES+ RL DY+DIL Sbjct: 69 SEKVLGKTLKALGVPRSEYVVATKCGRYADG-FDFSADRVTKSIDESLERLQLDYVDILQ 127 Query: 119 LHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVL---PQTRLDVILSY 175 HD+EFG ++Q++ E IPAL++LK+AGKIR+ G++GLPL +F VL P +DV+LSY Sbjct: 128 CHDIEFGSLDQIVNETIPALKKLKEAGKIRFIGITGLPLGIFTYVLDRIPAGMVDVVLSY 187 Query: 176 CHYSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEY 235 CHY +ND+T + G G+++ASP++MGLL+ P+WHPA AE+++AC+ AA Y Sbjct: 188 CHYGINDSTLEDLIPYLKSKGVGIISASPLAMGLLTENGPPEWHPASAELKSACRAAAVY 247 Query: 236 CRDKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEE----PMDEQLLTEVRD 291 C+++G +I+KLA+Q+S +N+ I + LV + + N+ E +E++L EV Sbjct: 248 CKERGNNISKLALQYSLSNKDISSVLVGMNSINQVEENVAAAVELATIGKNEKILAEVEA 307 Query: 292 ILKPIDGATWPSG 304 ILKP+ TWPSG Sbjct: 308 ILKPVKNQTWPSG 320 >ref|NP_195093.1| L-galactose dehydrogenase (L-GalDH) [Arabidopsis thaliana] pir||T04984 hypothetical protein T16L1.160 - Arabidopsis thaliana emb|CAA20580.1| putative protein [Arabidopsis thaliana] emb|CAB80084.1| putative protein [Arabidopsis thaliana] gb|AAK91395.1| AT4g33670/T16L1_160 [Arabidopsis thaliana] emb|CAD10386.1| L-galactose dehydrogenase [Arabidopsis thaliana] gb|AAL90998.1| AT4g33670/T16L1_160 [Arabidopsis thaliana] Length = 319 Score = 276 bits (706), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 139/313 (44%), Positives = 200/313 (63%), Gaps = 10/313 (3%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 ++ R LGNTGL+ V D+A+ TV A LGIN D SPYYG Sbjct: 4 IELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGTL 63 Query: 61 AETVLGEALQT--VPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILL 118 +E +LG+ L+ VPR YI++TK GRY EG FDFS ER+ +S +ES+ RL DY+DIL Sbjct: 64 SEKMLGKGLKALQVPRSDYIVATKCGRYKEG-FDFSAERVRKSIDESLERLQLDYVDILH 122 Query: 119 LHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVL---PQTRLDVILSY 175 HD+EFG ++Q++ E IPAL++LKQ GK R+ G++GLPL +F VL P +DVILSY Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182 Query: 176 CHYSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEY 235 CHY +ND+T + G G+++ASP++MGLL+ + P+WHPA E+++A + A + Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242 Query: 236 CRDKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEE----PMDEQLLTEVRD 291 C+ KG+ I KLA+Q+S AN++I + LV ++ + + N+ E MD++ L+EV Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302 Query: 292 ILKPIDGATWPSG 304 IL+P+ TWPSG Sbjct: 303 ILEPVKNLTWPSG 315 >gb|AAO18639.1| galactose dehydrogenase [Actinidia deliciosa] Length = 319 Score = 270 bits (690), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 145/313 (46%), Positives = 204/313 (65%), Gaps = 10/313 (3%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 + R LGNTGL+ V +AI +V A+ LGIN D SPYYG Sbjct: 4 LDLRPLGNTGLKLSSVGFGASPLGNVFGPVSDHDAIASVREALRLGINFFDTSPYYGGTL 63 Query: 61 AETVLGEALQT--VPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILL 118 +E VLG+AL+ VPR++YI+STK GRY EG FDFS ER+ +S +ES+ RL DY+DIL Sbjct: 64 SEKVLGKALKALGVPRNEYIVSTKCGRYAEG-FDFSAERVTKSFDESLERLQLDYVDILQ 122 Query: 119 LHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVL---PQTRLDVILSY 175 HD+EFG ++Q++ E IPAL++LK+AGKIR+ G++GLPL VF VL P +DVILSY Sbjct: 123 CHDIEFGSLDQIVNETIPALQKLKEAGKIRFIGITGLPLGVFTYVLDRVPPGTVDVILSY 182 Query: 176 CHYSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEY 235 CHYS+ND+T + G G+++ASP++MGLL+ P+WHPA E++AACQ AA + Sbjct: 183 CHYSINDSTLEDLLPYLKSKGVGVISASPLAMGLLTESGPPEWHPASPELKAACQAAAAH 242 Query: 236 CRDKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEE----PMDEQLLTEVRD 291 C++KG +I+KLA+Q+S +N+ I + LV + + N+ E DE+ ++E+ + Sbjct: 243 CKEKGRNISKLAMQYSLSNKDISSILVGMNSVKQVEENVAAANELATFGKDEKTVSEIEE 302 Query: 292 ILKPIDGATWPSG 304 ILKP+ TW SG Sbjct: 303 ILKPVKNQTWLSG 315 >ref|NP_812525.1| putative oxidoreductase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO78719.1| putative oxidoreductase [Bacteroides thetaiotaomicron VPI-5482] Length = 310 Score = 259 bits (663), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 131/307 (42%), Positives = 189/307 (61%), Gaps = 6/307 (1%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 M+Y ++G TG++ ++ E I V TAI+ G+N IDVSPYYG K Sbjct: 1 MQYHEIGKTGMKVSSLSFGASSLGGVFHDLKEKEGIQAVFTAIEAGMNFIDVSPYYGHYK 60 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGE---GEFDFSRERIIRSAEESMGRLGTDYLDIL 117 AETVLG+AL+ +PRD+Y LSTK GRYG+ +D+S +R S ESM RL D++D++ Sbjct: 61 AETVLGKALKDIPRDRYYLSTKVGRYGKDGVNTWDYSAKRATESVYESMERLNIDFIDLI 120 Query: 118 LLHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVL---PQTRLDVILS 174 +HD+EF + QV+ E +PAL EL++ G + + G++ L L + V+ P ++ +LS Sbjct: 121 NVHDIEFADLNQVVNETLPALVELREKGVVGHVGITDLQLENLKWVIDRSPSGTIESVLS 180 Query: 175 YCHYSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAE 234 +CHY L D E G++NASP+SMGLLS R VP WHPA + AC++A E Sbjct: 181 FCHYCLCDDKLADFLDYFESKEIGVINASPLSMGLLSERGVPVWHPAPKPLVDACRKAME 240 Query: 235 YCRDKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDILK 294 +C+ K I KLA+QFS +N +I TTL ST P N++ NI + EEP+D +L+ EVR+I+ Sbjct: 241 HCKAKNYPIEKLAMQFSVSNPKIATTLFSTTNPENVKKNIGFIEEPVDWELVREVREIIG 300 Query: 295 PIDGATW 301 +W Sbjct: 301 EQQRVSW 307 >gb|EAD84961.1| unknown [environmental sequence] Length = 312 Score = 251 bits (642), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 128/294 (43%), Positives = 178/294 (60%), Gaps = 1/294 (0%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 M+ R LG TGLE R++ DEA+ +V AID G+ LID SP+YG Sbjct: 18 MQTRPLGTTGLEVPILSFGASSLGQEFRKITIDEAMSSVRVAIDNGMRLIDTSPFYGRGM 77 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILLLH 120 +E +LG ALQ +PRD Y+L TK GRY G FDFS +R+ S + S+ RLGTD+LDI+L H Sbjct: 78 SEIMLGLALQDIPRDDYLLCTKLGRYDVGHFDFSAQRVAESVDISLHRLGTDHLDIMLCH 137 Query: 121 DVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVLPQTRLDVILSYCHYSL 180 D+EF P++Q+++E IPAL + +QAGKIR+ G SG P +F + QT +D +L+Y Y+L Sbjct: 138 DIEFVPMQQIVDETIPALRQAQQAGKIRFIGFSGYPQKIFRFICDQTDVDCVLNYNQYTL 197 Query: 181 NDTTXXXXXX-XXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEYCRDK 239 +T ++ G G+M+A P S LL++ +P W ++ A + A CR+ Sbjct: 198 QNTRFTDETVPYLQEKGIGVMHAGPFSARLLTDAPLPPWLKEPENVRKAAREAVACCREH 257 Query: 240 GEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDIL 293 G +AKLA+QFS AN I TT+ +A P NIR +W EE +DE LL V I Sbjct: 258 GSSLAKLALQFSVANPAIATTVAGSANPNNIRQWAEWLEEALDEDLLKTVLKIF 311 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 448,986,410 Number of extensions: 17929734 Number of successful extensions: 34027 Number of sequences better than 1.0e-05: 972 Number of HSP's gapped: 33288 Number of HSP's successfully gapped: 972 Length of query: 319 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 194 Effective length of database: 417,850,203 Effective search space: 81062939382 Effective search space used: 81062939382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)