BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0002.p (339 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_355742.1| AGR_C_5103p [Agrobacterium tumefaciens] >gi|179... 334 2e-90 gb|EAK46949.1| unknown [environmental sequence] 323 3e-87 gb|EAJ13457.1| unknown [environmental sequence] 304 2e-81 ref|NP_418778.1| putative oxidoreductase [Escherichia coli K12] ... 295 1e-78 sp|P39400|YJJN_ECOLI Hypothetical zinc-type alcohol dehydrogenas... 295 1e-78 >ref|NP_355742.1| AGR_C_5103p [Agrobacterium tumefaciens] ref|NP_533479.1| zinc-binding dehydrogenase [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||F97696 hypothetical zinc-type alcohol dehydrogenase-like protein in tsr-mdoB intergenic region [imported] - Agrobacterium tumefaciens (strain C58, Cereon) pir||AE2922 zinc-binding dehydrogenase Atu2814 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAK88527.1| AGR_C_5103p [Agrobacterium tumefaciens str. C58 (Cereon)] gb|AAL43795.1| zinc-binding dehydrogenase [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 336 Score = 334 bits (857), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 165/340 (48%), Positives = 229/340 (67%), Gaps = 7/340 (2%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 M I C+ G+ + DLP P +GE ++ IRRIG+CGTDLH + GNQPY +YPR++GH Sbjct: 1 MLAIFCDTPGQLTAK-DLPNPVRGEGEVLVRIRRIGVCGTDLHIFTGNQPYLSYPRIMGH 59 Query: 61 EVSGIIERIGDNPQG--LRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGM 118 E+SG +E + P G L AGD V++IPY+ CG+C ACL GK+NCC+ + V GVH DGGM Sbjct: 60 ELSGTVE---EAPAGSHLSAGDVVTIIPYMSCGKCNACLKGKSNCCRNIGVLGVHRDGGM 116 Query: 119 RERVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMA 178 E +S+P + +LK +GL+LDQAAM E LAIGAHAVRR + G +VL++GAGPIG+ V Sbjct: 117 VEYLSVPQQFVLKAEGLSLDQAAMTEFLAIGAHAVRRGAVEKGQKVLIVGAGPIGMAVAV 176 Query: 179 MARYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGN 238 A G +V +D +RL FCK HTV+ K++LS +T GNF V D+TGN Sbjct: 177 FAVLDGTEVTMIDGRTDRLDFCKDHLGVAHTVALGDGDKDRLSDITGGNFFDAVFDATGN 236 Query: 239 ISSMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIR 298 +ME F VGHGG+ V +V +I+F+DP+FH RE T+ GSRNAT +DFERV+ A+R Sbjct: 237 PKAMERGFSFVGHGGSYVLVSIVASDISFNDPEFHKRETTLLGSRNATADDFERVLRALR 296 Query: 299 DGYIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 +G + ++ I+HR + ++ +F P++ VIK +VE+ Sbjct: 297 EGKVP-EALITHRMTLADVPSKFAGLTDPKAGVIKGMVEV 335 >gb|EAK46949.1| unknown [environmental sequence] Length = 336 Score = 323 bits (829), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 162/337 (48%), Positives = 219/337 (64%), Gaps = 2/337 (0%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 M +ICE G +E P+PE + + ++ ++R+G+CGTDLH + GNQPY YPRV+GH Sbjct: 1 MLTVICETPGTLSAQER-PKPETGENDVLLRVKRVGVCGTDLHIFTGNQPYLAYPRVMGH 59 Query: 61 EVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMRE 120 E+SGI+E+ + GL AGDQV V+PYL CG+C AC GKTNCC ++V GVH DG E Sbjct: 60 ELSGIVEQAPPH-SGLAAGDQVFVMPYLSCGQCIACRRGKTNCCVNIQVLGVHRDGAFTE 118 Query: 121 RVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAMA 180 +S+P + + K +G+TLDQAAM+E LAIGAHAVRR + G++VLV+GAGPIG+ V+ Sbjct: 119 YLSVPAQFVHKAEGITLDQAAMVEFLAIGAHAVRRGDVKQGERVLVVGAGPIGMAVLTFC 178 Query: 181 RYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNIS 240 R G V A+D +RL FC+ A V +LSALT+G+F V D+TGN Sbjct: 179 RLRGATVTALDTRADRLDFCEQHLGAAAAVQIGDGDHARLSALTEGDFFDVVFDATGNPK 238 Query: 241 SMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRDG 300 +ME F + HGG V +V I+FSDP+FH RE T+ GSRNAT EDFE VI A+R G Sbjct: 239 AMERGFQFIAHGGRYVLVSIVADTISFSDPEFHKREATLLGSRNATLEDFETVIEAMRAG 298 Query: 301 YIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVE 337 I + +HR + ++ +F + L P V+KA+VE Sbjct: 299 NIPDRALNTHRLALADVPARFATLLDPAGGVVKAIVE 335 >gb|EAJ13457.1| unknown [environmental sequence] Length = 336 Score = 304 bits (779), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 155/338 (45%), Positives = 209/338 (61%), Gaps = 4/338 (1%) Query: 1 MKGIICEEVGKFVFREDLPEPELR-DGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLG 59 M +ICE G V R E +R GE ++ ++R+GICGTDLH + GNQPY YPRV+G Sbjct: 1 MLSVICESPG--VLRVQHRELPVRGSGEVLLRVQRVGICGTDLHIFTGNQPYLDYPRVMG 58 Query: 60 HEVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMR 119 HE+S ++ + GL AGD V V+PYL CG C AC G TNCC ++V GVH DG + Sbjct: 59 HELSAVVVE-AEAESGLGAGDAVYVMPYLSCGHCVACRHGNTNCCVNIKVLGVHRDGALA 117 Query: 120 ERVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAM 179 E +S+P + + K DG++LDQAAMLE LAIGAHAVRR+ I AG + LV+GAGPIG+ M Sbjct: 118 EYLSVPAQFVHKADGISLDQAAMLEFLAIGAHAVRRADIRAGQRALVVGAGPIGMAAMIF 177 Query: 180 ARYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNI 239 A+ G V +D +RL FC+ + V + E+L++LT+G + V D+TGN+ Sbjct: 178 AKLRGADVTCLDTRADRLAFCRQHLAVDAAVEVGEGDAERLASLTNGEYFDAVFDATGNL 237 Query: 240 SSMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRD 299 +M F V HGG T + +V+G++ FSDP+FH RE T+ SRNAT EDF V+ A+R Sbjct: 238 DAMNRGFEFVAHGGKYTLISIVRGHVAFSDPEFHKRETTLLASRNATAEDFATVLDAMRA 297 Query: 300 GYIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVE 337 G I + +HR E+ L V+KALVE Sbjct: 298 GRIPDRALNTHRMRLDEVPDALPRLLDAGQTVVKALVE 335 >ref|NP_418778.1| putative oxidoreductase [Escherichia coli K12] pir||S56585 L-iditol 2-dehydrogenase (EC 1.1.1.14) homolog yjjN - Escherichia coli (strain K-12) gb|AAA97256.1| ORF_o345 [Escherichia coli] gb|AAC77314.1| putative oxidoreductase [Escherichia coli K12] Length = 345 Score = 295 bits (754), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 154/348 (44%), Positives = 218/348 (62%), Gaps = 21/348 (6%) Query: 1 MKGIICEEVGKFVFRE-DLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLG 59 M +IC++ + V+++ ++P P D EA+I I+ +GICGTD+HA+ GNQP+F+YPRVLG Sbjct: 9 MNVLICQQPKELVWKQREIPIP--GDNEALIKIKSVGICGTDIHAWGGNQPFFSYPRVLG 66 Query: 60 HEVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMR 119 HE+ G I +G N L+ G QV+VIPY+ C +C AC SG+TNCC+K+ V GVH DGG Sbjct: 67 HEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGRTNCCEKISVIGVHQDGGFS 126 Query: 120 ERVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAM 179 E +S+PV N+L DG+ AA++EP AI AHAVRR+ I+ G+QVLV+GAGPIGLG A+ Sbjct: 127 EYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAI 186 Query: 180 ARYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEP---------KEQLSALTDGNFLP 230 A+ G +V+ D + R EH + L+ P QL A G+ Sbjct: 187 AKADGAQVVVADTSPAR---------REHVATRLELPLLDPSAEDFDAQLRAQFGGSLAQ 237 Query: 231 HVIDSTGNISSMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDF 290 VID+TGN +M L+ HGGT+ +VGL KG + FSDP+FH +E T+ GSRNAT EDF Sbjct: 238 KVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDF 297 Query: 291 ERVISAIRDGYIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 +V + +G I D ++HR F + +E + ++IK ++ Sbjct: 298 AKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF 345 >sp|P39400|YJJN_ECOLI Hypothetical zinc-type alcohol dehydrogenase-like protein yjjN Length = 337 Score = 295 bits (754), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 154/348 (44%), Positives = 218/348 (62%), Gaps = 21/348 (6%) Query: 1 MKGIICEEVGKFVFRE-DLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLG 59 M +IC++ + V+++ ++P P D EA+I I+ +GICGTD+HA+ GNQP+F+YPRVLG Sbjct: 1 MNVLICQQPKELVWKQREIPIP--GDNEALIKIKSVGICGTDIHAWGGNQPFFSYPRVLG 58 Query: 60 HEVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMR 119 HE+ G I +G N L+ G QV+VIPY+ C +C AC SG+TNCC+K+ V GVH DGG Sbjct: 59 HEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGRTNCCEKISVIGVHQDGGFS 118 Query: 120 ERVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAM 179 E +S+PV N+L DG+ AA++EP AI AHAVRR+ I+ G+QVLV+GAGPIGLG A+ Sbjct: 119 EYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAI 178 Query: 180 ARYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEP---------KEQLSALTDGNFLP 230 A+ G +V+ D + R EH + L+ P QL A G+ Sbjct: 179 AKADGAQVVVADTSPAR---------REHVATRLELPLLDPSAEDFDAQLRAQFGGSLAQ 229 Query: 231 HVIDSTGNISSMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDF 290 VID+TGN +M L+ HGGT+ +VGL KG + FSDP+FH +E T+ GSRNAT EDF Sbjct: 230 KVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDF 289 Query: 291 ERVISAIRDGYIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 +V + +G I D ++HR F + +E + ++IK ++ Sbjct: 290 AKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF 337 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 528,282,506 Number of extensions: 22685860 Number of successful extensions: 75700 Number of sequences better than 1.0e-05: 2261 Number of HSP's gapped: 72657 Number of HSP's successfully gapped: 2274 Length of query: 339 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 213 Effective length of database: 415,135,104 Effective search space: 88423777152 Effective search space used: 88423777152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)