BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0103.p (95 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_980410.1| phosphocarrier protein HPr [Bacillus cereus ATC... 80 1e-14 sp|O69250|PTHP_BACME Phosphocarrier protein HPr (Histidine-conta... 79 3e-14 ref|NP_658087.1| PTS-HPr, PTS HPr component phosphorylation site... 79 5e-14 ref|ZP_00183149.1| hypothetical protein Exigu144401 [Exiguobacte... 78 7e-14 ref|NP_470338.1| PTS phosphocarrier protein Hpr (histidine conta... 77 1e-13 >ref|NP_980410.1| phosphocarrier protein HPr [Bacillus cereus ATCC 10987] gb|AAS43018.1| phosphocarrier protein HPr [Bacillus cereus ATCC 10987] Length = 87 Score = 80.1 bits (196), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 41/88 (46%), Positives = 55/88 (62%), Gaps = 1/88 (1%) Query: 5 MQKTFRIIDEDGIHARPATALVNTATKFKGTESFAEAKGKKVTLKSILGVLSLGLEAGDT 64 M+K F++ + GIHARPAT LVNTA+KF G++ E GK V LKSI+GV+SLG++ G Sbjct: 1 MEKIFKVTSDSGIHARPATLLVNTASKF-GSDINLEYNGKNVNLKSIMGVMSLGIQQGAE 59 Query: 65 LTLITEGXXXXXXXXXXQEVMVKEGLGE 92 + + G +E M EGLGE Sbjct: 60 IKITANGDDAAQALAAIEETMKNEGLGE 87 >sp|O69250|PTHP_BACME Phosphocarrier protein HPr (Histidine-containing protein) emb|CAA06331.1| HPr protein [Bacillus megaterium] Length = 88 Score = 79.0 bits (193), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 42/89 (47%), Positives = 55/89 (61%), Gaps = 1/89 (1%) Query: 4 IMQKTFRIIDEDGIHARPATALVNTATKFKGTESFAEAKGKKVTLKSILGVLSLGLEAGD 63 + QKTF + + GIHARPAT LV A+KF + E GK V LKSI+GV+SLG++ G Sbjct: 1 MAQKTFTVTADSGIHARPATTLVQAASKFDSDINL-EFNGKTVNLKSIMGVMSLGIQKGA 59 Query: 64 TLTLITEGXXXXXXXXXXQEVMVKEGLGE 92 T+T+ EG ++ M KEGLGE Sbjct: 60 TITISAEGSDEADALAALEDTMSKEGLGE 88 >ref|NP_658087.1| PTS-HPr, PTS HPr component phosphorylation site [Bacillus anthracis A2012] ref|NP_833767.1| Phosphocarrier protein HPr [Bacillus cereus ATCC 14579] ref|NP_846502.1| phosphocarrier protein HPr [Bacillus anthracis str. Ames] gb|AAG49026.1| PtsH [Bacillus thuringiensis] gb|AAP10968.1| Phosphocarrier protein HPr [Bacillus cereus ATCC 14579] gb|AAP27988.1| phosphocarrier protein HPr [Bacillus anthracis str. Ames] Length = 87 Score = 78.6 bits (192), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 40/88 (45%), Positives = 54/88 (61%), Gaps = 1/88 (1%) Query: 5 MQKTFRIIDEDGIHARPATALVNTATKFKGTESFAEAKGKKVTLKSILGVLSLGLEAGDT 64 M+K F++ + GIHARPAT LVNTA+KF G++ E GK V LKSI+GV+SLG++ Sbjct: 1 MEKIFKVTSDSGIHARPATLLVNTASKF-GSDINLEYNGKNVNLKSIMGVMSLGIQQNAE 59 Query: 65 LTLITEGXXXXXXXXXXQEVMVKEGLGE 92 + + G +E M EGLGE Sbjct: 60 IKITANGDDAAQALAAIEETMKNEGLGE 87 >ref|ZP_00183149.1| hypothetical protein Exigu144401 [Exiguobacterium sp. 255-15] Length = 94 Score = 77.8 bits (190), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 39/90 (43%), Positives = 56/90 (62%), Gaps = 1/90 (1%) Query: 3 IIMQKTFRIIDEDGIHARPATALVNTATKFKGTESFAEAKGKKVTLKSILGVLSLGLEAG 62 +IM+KTF++I + GIHARPAT LVNTA+KF+ + E GK V LKSI+GVLSLG+ Sbjct: 6 LIMEKTFKVIADSGIHARPATQLVNTASKFQSDINL-EYNGKTVNLKSIMGVLSLGIAKD 64 Query: 63 DTLTLITEGXXXXXXXXXXQEVMVKEGLGE 92 + ++ G +++ EGL + Sbjct: 65 SDIKIVANGDDASEAITTLSDMLTSEGLAQ 94 >ref|NP_470338.1| PTS phosphocarrier protein Hpr (histidine containing protein) [Listeria innocua] ref|NP_464527.1| PTS phosphocarrier protein Hpr (histidine containing protein) [Listeria monocytogenes EGD-e] sp|O31148|PTHP_LISMO Phosphocarrier protein HPr (Histidine-containing protein) pir||AB1200 PTS phosphocarrier protein Hpr (histidine containing protein) [imported] - Listeria monocytogenes (strain EGD-e) pir||AH1557 PTS phosphocarrier protein Hpr (histidine containing protein) [imported] - Listeria innocua (strain Clip11262) gb|AAC36127.1| HPr [Listeria monocytogenes] emb|CAC99080.1| PTS phosphocarrier protein Hpr (histidine containing protein) [Listeria monocytogenes] emb|CAC96232.1| PTS phosphocarrier protein Hpr (histidine containing protein) [Listeria innocua] Length = 88 Score = 77.0 bits (188), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 43/87 (49%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Query: 6 QKTFRIIDEDGIHARPATALVNTATKFKGTESFAEAKGKKVTLKSILGVLSLGLEAGDTL 65 Q +F +IDE GIHARPAT LV A+K+ ++ E GKKV LKSI+GV+SLG+ G + Sbjct: 3 QASFVVIDETGIHARPATLLVQAASKYS-SDVQIEYTGKKVNLKSIMGVMSLGIGKGADI 61 Query: 66 TLITEGXXXXXXXXXXQEVMVKEGLGE 92 T+ TEG EV+ KEGL E Sbjct: 62 TIYTEGSDEKEAIEGLTEVLKKEGLAE 88 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.134 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 105,876,447 Number of extensions: 3105559 Number of successful extensions: 5539 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 5626 Number of HSP's successfully gapped: 49 Length of query: 95 Length of database: 757,237,578 Length adjustment: 63 Effective length of query: 32 Effective length of database: 586,186,341 Effective search space: 18757962912 Effective search space used: 18757962912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 120 (50.8 bits)