BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0080.p (406 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181462.1| hypothetical protein Mther054601 [Moorella th... 528 e-149 ref|NP_244216.1| aspartate aminotransferase [Bacillus halodurans... 502 e-141 emb|CAB15129.2| alanine transaminase [Bacillus subtilis subsp. s... 484 e-135 ref|ZP_00061526.1| COG0436: Aspartate/tyrosine/aromatic aminotra... 474 e-132 ref|NP_834574.1| Aromatic amino acid aminotransferase [Bacillus ... 473 e-132 >ref|ZP_00181462.1| hypothetical protein Mther054601 [Moorella thermoacetica ATCC 39073] Length = 404 Score = 528 bits (1360), Expect = e-149, Method: Compositional matrix adjust. Identities = 242/387 (62%), Positives = 308/387 (79%) Query: 13 GKSMHSYLAPLVQQIQPSGIRKFFDLAAGSKDIISLGVGEPDFKTPWHVREACVYSLERG 72 G ++ P+V+ + PSGIR+FF+L A +K +ISLGVGEPDF TPWH+REACV SLERG Sbjct: 6 GFEARRFITPVVRDLPPSGIRRFFELVASTKGVISLGVGEPDFVTPWHIREACVQSLERG 65 Query: 73 FTGYTSNAGMPELREGIAEYLHTRFAVEYNPANEIIATVGGSEAIDLALRALISPGDEIL 132 +T YTSN G+PELR IA+YL RF + Y+P +I+ T+G SEA+DLALR +++PGDE+L Sbjct: 66 YTMYTSNYGLPELRRAIADYLAWRFGLTYDPMKQIMVTIGASEAVDLALRTVLNPGDEVL 125 Query: 133 IPEPCYISYSPITAIGGGIPVGIETFGENNFKLTAEGLEAKITPRSKILILCYPSNPTGA 192 IPEPCY+SY PIT + GGIPV I T + F LTA LE ITPRSK+LILC+P+NPTGA Sbjct: 126 IPEPCYVSYQPITQLAGGIPVPIPTTMTDGFALTAARLEHYITPRSKVLILCFPNNPTGA 185 Query: 193 IMSREDWEPIARIVEKHDLIVISDEIYAELTYSGNHVSFASLPGMKDRTILVSGFSKAFA 252 ++SRE+ + IAR+VEK++L+VISDEIYAEL Y G +SFASLPGM++RTILVSGFSKAFA Sbjct: 186 VLSREEMQAIARLVEKYNLLVISDEIYAELRYEGQPLSFASLPGMQERTILVSGFSKAFA 245 Query: 253 MTGWRMGYACGHPDLIAAMLKIHQYTVMCAPSMGQVAALEALTNGMEEKDRMVDSYNQRR 312 MTGWR+GY HPD +AAM+KIHQYT++CAP MGQ+AALEAL +G ++ +RMV+ Y+QRR Sbjct: 246 MTGWRIGYVAAHPDFLAAMVKIHQYTILCAPVMGQMAALEALRHGRQDVERMVEQYDQRR 305 Query: 313 RLIVKGLRDAGLECHEPQGAFYAFPSIRRTGLTSDEFAQRLLLEHKVAAVPGSVFGLGGE 372 RL+ LR+ GL+C EP+GAFY FPSI TGL S FA+ LL E KVA VPG+ FG GE Sbjct: 306 RLVYSRLREMGLDCFEPRGAFYIFPSIAATGLDSVTFAEELLKEEKVAVVPGTAFGASGE 365 Query: 373 GYLRCSYATSVSQLNEAVERIGAFVSQ 399 G++RCSYA S++ L EA+ R+ FV++ Sbjct: 366 GFIRCSYAASLADLTEAMNRMERFVAR 392 >ref|NP_244216.1| aspartate aminotransferase [Bacillus halodurans] pir||F84068 aspartate aminotransferase BH3350 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07069.1| aspartate aminotransferase [Bacillus halodurans] Length = 393 Score = 502 bits (1293), Expect = e-141, Method: Compositional matrix adjust. Identities = 227/391 (58%), Positives = 305/391 (78%) Query: 12 TGKSMHSYLAPLVQQIQPSGIRKFFDLAAGSKDIISLGVGEPDFKTPWHVREACVYSLER 71 + S + L+ VQ IQPSGIR+FFDLA+ ++IISLGVGEPDF TPW+VREA + S+ER Sbjct: 3 SSTSQRTRLSTRVQSIQPSGIRRFFDLASKMENIISLGVGEPDFVTPWNVREASISSMER 62 Query: 72 GFTGYTSNAGMPELREGIAEYLHTRFAVEYNPANEIIATVGGSEAIDLALRALISPGDEI 131 GFT Y++NAG+ ELRE I+ YL+ RF + Y+P +EI+ TVG SEAID+ +RA+I GDE+ Sbjct: 63 GFTAYSANAGIIELREAISRYLYERFHIGYDPESEILVTVGASEAIDIGMRAIIDEGDEV 122 Query: 132 LIPEPCYISYSPITAIGGGIPVGIETFGENNFKLTAEGLEAKITPRSKILILCYPSNPTG 191 ++ EP ++SY+P+ + GG+PV + T + +F++T +EA ITPR+K +ILC+P+NPTG Sbjct: 123 IVVEPSFVSYAPLVTMAGGVPVPVGTHIDTDFQVTPAQIEAAITPRTKAIILCFPNNPTG 182 Query: 192 AIMSREDWEPIARIVEKHDLIVISDEIYAELTYSGNHVSFASLPGMKDRTILVSGFSKAF 251 +IM +E+ E +A+++ +DLIV SDEIYAELTY G HVS AS+ GM++RT+L+SGFSKAF Sbjct: 183 SIMGKEELEAVAKVISGNDLIVFSDEIYAELTYDGTHVSLASMDGMRERTVLISGFSKAF 242 Query: 252 AMTGWRMGYACGHPDLIAAMLKIHQYTVMCAPSMGQVAALEALTNGMEEKDRMVDSYNQR 311 AMTGWR+GY C D+++AMLKIHQY++MCAP+M Q ALEAL GM++ RMV SY QR Sbjct: 243 AMTGWRLGYVCAPDDILSAMLKIHQYSLMCAPTMAQHGALEALETGMDDVHRMVQSYRQR 302 Query: 312 RRLIVKGLRDAGLECHEPQGAFYAFPSIRRTGLTSDEFAQRLLLEHKVAAVPGSVFGLGG 371 R +VK + GL C P GAFYAFPS++ TGLTS+EFA+RLL+E VA VPG+VFG GG Sbjct: 303 RNFVVKTFTEIGLTCPMPGGAFYAFPSVKETGLTSEEFAERLLMEEHVAVVPGNVFGEGG 362 Query: 372 EGYLRCSYATSVSQLNEAVERIGAFVSQLER 402 EG++RCSYATS+ L A+ERIG FV + ++ Sbjct: 363 EGHIRCSYATSMEHLETALERIGRFVQKCKQ 393 >emb|CAB15129.2| alanine transaminase [Bacillus subtilis subsp. subtilis str. 168] Length = 386 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 219/384 (57%), Positives = 293/384 (76%) Query: 16 MHSYLAPLVQQIQPSGIRKFFDLAAGSKDIISLGVGEPDFKTPWHVREACVYSLERGFTG 75 M SYL+ VQQI+PSGIRKFFDLAA + +ISLGVGEPDF T W+VREA + SLE+G+T Sbjct: 1 MTSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTS 60 Query: 76 YTSNAGMPELREGIAEYLHTRFAVEYNPANEIIATVGGSEAIDLALRALISPGDEILIPE 135 YT+NAG+ LRE I+ YL RF + Y+P NE+I TVG S+A+D+A+RA+++PG+E++IPE Sbjct: 61 YTANAGLYSLREEISRYLSNRFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPE 120 Query: 136 PCYISYSPITAIGGGIPVGIETFGENNFKLTAEGLEAKITPRSKILILCYPSNPTGAIMS 195 PC+++Y + ++ GGIPV + T + FK TA EA +T ++K +++C PSNPTG++ S Sbjct: 121 PCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVTEKTKAILICSPSNPTGSVYS 180 Query: 196 REDWEPIARIVEKHDLIVISDEIYAELTYSGNHVSFASLPGMKDRTILVSGFSKAFAMTG 255 +E+ IA +KHD+IV++DEIYAELTY S A+LPGMK+RT+++SGFSKAFAMTG Sbjct: 181 KEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG 240 Query: 256 WRMGYACGHPDLIAAMLKIHQYTVMCAPSMGQVAALEALTNGMEEKDRMVDSYNQRRRLI 315 WR+G+A L AMLKIHQY +MCAP+M Q AALE L NGME+ ++M SY +RR L Sbjct: 241 WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLF 300 Query: 316 VKGLRDAGLECHEPQGAFYAFPSIRRTGLTSDEFAQRLLLEHKVAAVPGSVFGLGGEGYL 375 V+ L + GL CH P GAFYAFPSI+ G++S++FA+ LL + KVA VPGSVFG GEGY+ Sbjct: 301 VESLNEIGLSCHHPGGAFYAFPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYI 360 Query: 376 RCSYATSVSQLNEAVERIGAFVSQ 399 RCSYATS+ QL EA+ R+ F+ + Sbjct: 361 RCSYATSIEQLQEALVRMKRFLHK 384 >ref|ZP_00061526.1| COG0436: Aspartate/tyrosine/aromatic aminotransferase [Clostridium thermocellum ATCC 27405] Length = 390 Score = 474 bits (1221), Expect = e-132, Method: Compositional matrix adjust. Identities = 219/385 (56%), Positives = 286/385 (74%), Gaps = 3/385 (0%) Query: 22 PLVQQIQPSGIRKFFDLAAGSKDIISLGVGEPDFKTPWHVREACVYSLERGFTGYTSNAG 81 P V+Q+ PSGIRKFFDL KD ISL +GEPDF TPW++REA +YSLE+G T Y+ NAG Sbjct: 9 PTVRQVPPSGIRKFFDLVNEMKDAISLSIGEPDFVTPWNIREAGIYSLEKGHTHYSPNAG 68 Query: 82 MPELREGIAEYLHTRFAVEYNPANEIIATVGGSEAIDLALRALISPGDEILIPEPCYISY 141 ELRE I +Y ++ ++Y+P ++I TVGGSE ID+ALRAL+ PGDE++IPEPC+++Y Sbjct: 69 FMELREEICKYQERKYNLKYDPKTQVIVTVGGSEGIDIALRALVGPGDEVIIPEPCFVAY 128 Query: 142 SPITAIGGGIPVGIETFGENNFKLTAEGLEAKITPRSKILILCYPSNPTGAIMSREDWEP 201 P T G PV + EN F+LT E LE IT R+K+LIL YP+NPTGA+M ++D E Sbjct: 129 KPCTIFTGATPVIVNLSAENEFRLTPELLEKAITDRTKVLILPYPNNPTGAVMEKKDLEA 188 Query: 202 IARIVEKHDLIVISDEIYAELTYSGNHVSFASLPGMKDRTILVSGFSKAFAMTGWRMGYA 261 I +++ D+IVISDEIY+ELTY HVS AS P MKD+T++++GFSKA+AMTGWR+GY Sbjct: 189 IVEVLKDKDIIVISDEIYSELTYGVKHVSIASFPEMKDKTVVINGFSKAYAMTGWRLGYC 248 Query: 262 CGHPDLIAAMLKIHQYTVMCAPSMGQVAALEALTNGMEEKDRMVDSYNQRRRLIVKGLRD 321 GHPDLI+ M KIHQY +MC+P+ Q AA+EAL N ++ + MV YN+RRR++V+G R Sbjct: 249 LGHPDLISQMYKIHQYAIMCSPTTAQYAAIEALRNSDDDVEAMVREYNRRRRIMVEGFRS 308 Query: 322 AGLECHEPQGAFYAFPSIRRTGLTSDEFAQRLLLEHKVAAVPGSVFGLGGEGYLRCSYAT 381 AGLEC EP GAFY FP I TG+TS EF++RLL+E KVA VPG+ FG GEGY+R YA+ Sbjct: 309 AGLECFEPLGAFYVFPCIESTGMTSYEFSERLLMEEKVAVVPGTAFGECGEGYVRSCYAS 368 Query: 382 SVSQLNEAVERIGAFVSQLERERTE 406 S+ + EA++RI FV ER R + Sbjct: 369 SMDNIIEAMKRIKRFV---ERNRKK 390 >ref|NP_834574.1| Aromatic amino acid aminotransferase [Bacillus cereus ATCC 14579] gb|AAP11775.1| Aromatic amino acid aminotransferase [Bacillus cereus ATCC 14579] Length = 396 Score = 473 bits (1218), Expect = e-132, Method: Compositional matrix adjust. Identities = 219/380 (57%), Positives = 289/380 (76%) Query: 25 QQIQPSGIRKFFDLAAGSKDIISLGVGEPDFKTPWHVREACVYSLERGFTGYTSNAGMPE 84 + ++PSGIRKFFDLAA K +ISLGVGEPDF TPW+VR+AC+ SLE+G+T YT+NAG+ E Sbjct: 11 ESLKPSGIRKFFDLAANMKGVISLGVGEPDFVTPWNVRQACIRSLEQGYTSYTANAGLLE 70 Query: 85 LREGIAEYLHTRFAVEYNPANEIIATVGGSEAIDLALRALISPGDEILIPEPCYISYSPI 144 LR+ IA+YL +FAV Y+P++EII TVG S+A+D+A+RA+I+P DE+LI EP ++SY+P+ Sbjct: 71 LRQEIAKYLKKQFAVSYDPSDEIIVTVGASQALDVAMRAIINPDDEVLIVEPSFVSYAPL 130 Query: 145 TAIGGGIPVGIETFGENNFKLTAEGLEAKITPRSKILILCYPSNPTGAIMSREDWEPIAR 204 + GG+PV +ET EN FK+ E +EA IT ++K ++LC P+NPTGA++++ + E IA Sbjct: 131 VTLAGGVPVPVETKLENEFKVQPEQIEAAITAKTKAILLCSPNNPTGAMLNKSELEEIAV 190 Query: 205 IVEKHDLIVISDEIYAELTYSGNHVSFASLPGMKDRTILVSGFSKAFAMTGWRMGYACGH 264 IVEK++LIV+SDEIYAEL Y + SFAS+ M++ TIL+SGFSK FAMTGWR+G Sbjct: 191 IVEKYNLIVLSDEIYAELVYDEAYTSFASIKNMREHTILISGFSKGFAMTGWRLGMIAAP 250 Query: 265 PDLIAAMLKIHQYTVMCAPSMGQVAALEALTNGMEEKDRMVDSYNQRRRLIVKGLRDAGL 324 MLKIHQY++MCAP+M Q AALEAL G +E RM DSY +RR + + GL Sbjct: 251 VQFSELMLKIHQYSMMCAPTMSQFAALEALRAGNDEVIRMRDSYKKRRNFMTTSFNEMGL 310 Query: 325 ECHEPQGAFYAFPSIRRTGLTSDEFAQRLLLEHKVAAVPGSVFGLGGEGYLRCSYATSVS 384 CH P GAFY FPSI TGL+S EFA++LLLE KVA VPGSVFG GEG++RCSYATS+ Sbjct: 311 TCHVPGGAFYVFPSISSTGLSSAEFAEQLLLEEKVAVVPGSVFGESGEGFIRCSYATSLE 370 Query: 385 QLNEAVERIGAFVSQLERER 404 QL EA++R+ FV +R + Sbjct: 371 QLMEAMKRMERFVENKKRTK 390 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 614,112,765 Number of extensions: 26215305 Number of successful extensions: 69242 Number of sequences better than 1.0e-05: 3591 Number of HSP's gapped: 62502 Number of HSP's successfully gapped: 3592 Length of query: 406 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 278 Effective length of database: 409,704,906 Effective search space: 113897963868 Effective search space used: 113897963868 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)