BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0069.p (1950 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_622644.1| Glycerophosphoryl diester phosphodiesterase [Th... 132 1e-28 ref|ZP_00143150.1| Glycerophosphoryl diester phosphodiesterase [... 108 1e-21 ref|NP_845028.1| glycerophosphoryl diester phosphodiesterase, pu... 108 2e-21 ref|NP_244567.1| glycerophosphoryl diester phosphodiesterase [Ba... 107 2e-21 ref|NP_979008.1| glycerophosphoryl diester phosphodiesterase, pu... 105 1e-20 >ref|NP_622644.1| Glycerophosphoryl diester phosphodiesterase [Thermoanaerobacter tengcongensis] gb|AAM24248.1| Glycerophosphoryl diester phosphodiesterase [Thermoanaerobacter tengcongensis] Length = 243 Score = 132 bits (331), Expect = 1e-28, Method: Composition-based stats. Identities = 79/238 (33%), Positives = 127/238 (53%), Gaps = 13/238 (5%) Query: 1139 KVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGS 1198 K +I HRG PENTI + + A++ GAD IE D+ LT DGHL++ H ++ TT+G Sbjct: 2 KTLVIAHRGDSKNVPENTIAAFKRAMELGADGIELDVQLTKDGHLVVIHDETVDRTTNGE 61 Query: 1199 GPVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSRGRLVYAEIKTQT---PEA 1255 G V++++LE++K +A + E F R+PTL E EL + LV EIK+ P Sbjct: 62 GFVKDFTLEEIKKLDAGIKFGEKFAGERIPTLYEVFELIGDKDFLVNIEIKSGIVLYPGI 121 Query: 1256 VDAFVRTVKEHKAEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLRDTL 1315 + ++ +KE+ E+ V + SF L + K+ P + GLL + L + Sbjct: 122 EEKLIKAIKEYNFEERVIISSFNHYSLRDVKKMAPHLKIGLL---------YQCGLVEPW 172 Query: 1316 KTVSKLN-LTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 ++ + + Y + PE +E K G+ + PWT++++ED+ R +K G GI TD Sbjct: 173 HMALRMEAYSLHPFYFNIIPELVEGCKKNGVKLFPWTVDRKEDMERMIKAGVDGIITD 230 >ref|ZP_00143150.1| Glycerophosphoryl diester phosphodiesterase [Fusobacterium nucleatum subsp. vincentii ATCC 49256] gb|EAA25277.1| Glycerophosphoryl diester phosphodiesterase [Fusobacterium nucleatum subsp. vincentii ATCC 49256] Length = 244 Score = 108 bits (271), Expect = 1e-21, Method: Composition-based stats. Identities = 69/218 (31%), Positives = 113/218 (51%), Gaps = 11/218 (5%) Query: 1144 GHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGSGPVEN 1203 HRG PENTI + + A++ GAD IE D+ LT DG ++I H ++ TTDG G V + Sbjct: 6 AHRGFSGKYPENTILAFQKAIEVGADGIELDVQLTKDGEVVIIHDETIDRTTDGKGYVVD 65 Query: 1204 YSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSRGRLVYAEIKTQTPEAV---DAFV 1260 Y+ ++L ++A+ Y + ++PTL E EL + + E+KT E + + Sbjct: 66 YTYKELSKFDASYIYRGKMGVNKIPTLKEYFELVKDLDFITNIELKTGINEYLGIEEKVY 125 Query: 1261 RTVKEHKAEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLRDTLKTVSK 1320 + +KE+K E V + SF + R+ K+ PE+ G L SE+ + + T S Sbjct: 126 QLIKEYKLEKKVIISSFNHFSILRMKKIAPELKCGFL-----SEDWI---IDAGAYTASH 177 Query: 1321 LNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDV 1358 F+ ++ L PE + K + I+ WT+N+ ED+ Sbjct: 178 KIECFHPRFNNLIPEVVAELKKNNIEINTWTVNREEDI 215 >ref|NP_845028.1| glycerophosphoryl diester phosphodiesterase, putative [Bacillus anthracis str. Ames] gb|AAP26514.1| glycerophosphoryl diester phosphodiesterase, putative [Bacillus anthracis str. Ames] Length = 287 Score = 108 bits (269), Expect = 2e-21, Method: Composition-based stats. Identities = 66/241 (27%), Positives = 119/241 (49%), Gaps = 15/241 (6%) Query: 1143 IGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGSGPVE 1202 I HRG + +PE+TI + +L D+IE D+ +T DGHL+ H + TT+G+G V+ Sbjct: 42 IAHRGASAYAPEHTIAAYKLGQQLKGDYIEIDLQMTKDGHLVAMHDETVNRTTNGTGLVK 101 Query: 1203 NYSLEQLKTYNANKTYPEGFPLV--------RMPTLDEQLELARSRGRLVYAEIKT--QT 1252 ++LE+LK NA TY + +P + ++PTL+E +E+ Y E K+ + Sbjct: 102 EHTLEELKQLNAGSTYNKKYPNLAKKEYENAKVPTLEEVIEVFGHDANY-YIETKSPDEY 160 Query: 1253 PEAVDAFVRTVKEHKAEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLR 1312 P + + + ++ +D V + SF L++L+ L IP L Y + E Sbjct: 161 PGMEEKLLEIINHYEIQDKVIIQSFSEVSLQKLHSLHVNIPLAQLLSYKKAVQLTESE-- 218 Query: 1313 DTLKTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 +K + +Y + +++ K GL + P+T++ D+ + + G G+ T+ Sbjct: 219 --IKKYKTYCIGLGMNYKYIDAAYVKRIKKHGLEVHPFTVDNETDMKKLILWGVDGMFTN 276 Query: 1373 Y 1373 Y Sbjct: 277 Y 277 >ref|NP_244567.1| glycerophosphoryl diester phosphodiesterase [Bacillus halodurans] pir||D84112 glycerophosphoryl diester phosphodiesterase glpQ [imported] - Bacillus halodurans (strain C-125) dbj|BAB07419.1| glycerophosphoryl diester phosphodiesterase [Bacillus halodurans] Length = 284 Score = 107 bits (268), Expect = 2e-21, Method: Composition-based stats. Identities = 70/241 (29%), Positives = 122/241 (50%), Gaps = 10/241 (4%) Query: 1138 RKVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDG 1197 +K+ I HRG +PENT+ + + D D+IE D+ +T DG L++ H ++ TT+G Sbjct: 37 QKMINIAHRGASGHAPENTMAAFQKGFDMKVDYIEFDVHMTKDGELVLMHDLTVDRTTNG 96 Query: 1198 SGPVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSR-GRLVYAEIKTQTPEAV 1256 G V + +L+++K ++A + E F ++PT +E L++ R + G L+ + + P Sbjct: 97 HGYVSDLTLDEIKQFDAGSWFHEDFVGEKVPTFEETLDVFRGKVGLLIELKSPERYPGME 156 Query: 1257 DAFVRTVKEHKAEDIVN----VMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLR 1312 + +KE K + N + SF +++ +L+P IP G+L G S NV Sbjct: 157 EKVAEALKERKMDRSSNHKVIIQSFNHESMKKSKELLPHIPHGVLAG--PSWANVTD--- 211 Query: 1313 DTLKTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 + L+ S FN + + + PE + G+ I P+T +E R +L GI TD Sbjct: 212 EQLEEFSTYADYFNPNMTIVTPELVSRIHAVGMKIFPYTSRTQEQANRLFELKVDGIITD 271 Query: 1373 Y 1373 + Sbjct: 272 F 272 >ref|NP_979008.1| glycerophosphoryl diester phosphodiesterase, putative [Bacillus cereus ATCC 10987] gb|AAS41616.1| glycerophosphoryl diester phosphodiesterase, putative [Bacillus cereus ATCC 10987] Length = 287 Score = 105 bits (262), Expect = 1e-20, Method: Composition-based stats. Identities = 66/241 (27%), Positives = 120/241 (49%), Gaps = 15/241 (6%) Query: 1143 IGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGSGPVE 1202 I HRG + +PE+TI + L D+IE D+ +T DG+L+ H L TT+G+G V+ Sbjct: 42 IAHRGASAYAPEHTIAAYTLGQQLKGDYIEIDLQMTKDGYLVAMHDETLNRTTNGTGLVK 101 Query: 1203 NYSLEQLKTYNANKTYPEGFPLV--------RMPTLDEQLELARSRGRLVYAEIKT--QT 1252 ++LE++K NA TY + +P + ++PTL+E +E+ Y E K+ + Sbjct: 102 EHTLEEIKQLNAGSTYNKKYPNLAKKEYENAKVPTLEEVIEVFGHDANY-YIETKSPDEY 160 Query: 1253 PEAVDAFVRTVKEHKAEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLR 1312 PE + + + ++ +D V + SF L++++ L IP L Y + E Sbjct: 161 PEMEEKLLEIINHYEIQDKVIIQSFSEESLQKIHNLNVNIPLVQLLSYKKAVQLTELE-- 218 Query: 1313 DTLKTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 +K + +Y + +++ K GL + P+T++ +D+ + L G G+ T+ Sbjct: 219 --IKKYKTYCIGLGMNYKYIDSAYVKRIKKHGLEVHPFTVDNEQDMKKLLLWGVDGMFTN 276 Query: 1373 Y 1373 Y Sbjct: 277 Y 277 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,668,311,115 Number of extensions: 111678377 Number of successful extensions: 218512 Number of sequences better than 1.0e-05: 370 Number of HSP's gapped: 218353 Number of HSP's successfully gapped: 372 Length of query: 1950 Length of database: 757,237,578 Length adjustment: 141 Effective length of query: 1809 Effective length of database: 374,408,619 Effective search space: 677305191771 Effective search space used: 677305191771 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)