BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0061.p (509 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAA76421.2| alpha-L-arabinofuranosidase [Thermobacillus xyla... 761 0.0 ref|NP_242740.1| alpha-L-arabinosidase; beta-xylosidase / alpha-... 718 0.0 gb|AAN05450.1| alpha-L-arabinofuranosidase ArfA [Clostridium cel... 697 0.0 gb|AAC28125.1| alpha-L-arabinofuranosidase [Clostridium stercora... 678 0.0 ref|NP_390729.1| beta-xylosidase; alpha-L-arabinosidase [Bacillu... 675 0.0 >emb|CAA76421.2| alpha-L-arabinofuranosidase [Thermobacillus xylanilyticus] Length = 496 Score = 761 bits (1964), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/497 (72%), Positives = 413/497 (83%), Gaps = 3/497 (0%) Query: 10 IEMAQRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEAL 69 + +A RVV++ D KGTINRNIYGHFSEHLGRCIYEG+WVGEDSPIPNT GIRNDV+EAL Sbjct: 1 MNVASRVVVNADRVKGTINRNIYGHFSEHLGRCIYEGLWVGEDSPIPNTNGIRNDVLEAL 60 Query: 70 KEIKVPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAML 129 K++K+PVLRWPGGCFADEYHWKDG+G RE RKRM+NTHWGG +ENNHFGTHEFM LC +L Sbjct: 61 KQMKIPVLRWPGGCFADEYHWKDGVGPREKRKRMVNTHWGGVIENNHFGTHEFMMLCELL 120 Query: 130 ECEPYINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGC 189 CEPYI+GNVGSGTVQEMSEWVEY+TF+G SPMA R +NGRE+ W +KY+GVGNENWGC Sbjct: 121 GCEPYISGNVGSGTVQEMSEWVEYITFDGESPMANWRRENGREKPWRIKYWGVGNENWGC 180 Query: 190 GGNMRPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLH 249 GGNMR EYYADLYRQ+QTY+RNYGDN++H+IACG N DY W EVLM++A FM ++LH Sbjct: 181 GGNMRAEYYADLYRQFQTYLRNYGDNKLHKIACGANTADYHWTEVLMKQAAPFMHGLSLH 240 Query: 250 YYTVPGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEW 309 YYTVPG WE KG ATGF T E++ TL+KAL M+ LVT+HS IMD YDP+KR+ LIVDEW Sbjct: 241 YYTVPGP-WEKKGPATGFTTDEWWVTLKKALFMDRLVTKHSAIMDVYDPDKRIDLIVDEW 299 Query: 310 GTWYDVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTE 369 GTWYDVEPGTNPGFLYQQNSIRDALVAG TL+IFH+H DRVRMANIAQ +NVLQ+VILTE Sbjct: 300 GTWYDVEPGTNPGFLYQQNSIRDALVAGATLHIFHRHCDRVRMANIAQLVNVLQSVILTE 359 Query: 370 GEKMLLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSL 429 GE+MLLTPTYHVFNM+KVHQDAELLD T +S + E+P+VS SAS A+G IH+SL Sbjct: 360 GERMLLTPTYHVFNMFKVHQDAELLD-TWESVERTGPEGELPKVSVSASRAADGKIHISL 418 Query: 430 CNLNHASAAVLPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGD 489 CNL+ + A + +ELRGL G V GTTL ID HN+F EPE V P PF FKLEG Sbjct: 419 CNLDFETGASVDIELRGLNG-GVSATGTTLTSGRIDGHNTFDEPERVKPAPFRDFKLEGG 477 Query: 490 TVTVELPAMSVTVLEIT 506 + LP MSVTVLE+T Sbjct: 478 HLNASLPPMSVTVLELT 494 >ref|NP_242740.1| alpha-L-arabinosidase; beta-xylosidase / alpha-L-arabinosidase [Bacillus halodurans] pir||B83884 beta-xylosidase / alpha-L-arabinosidase xsa [imported] - Bacillus halodurans (strain C-125) dbj|BAB05593.1| alpha-L-arabinosidase [Bacillus halodurans] Length = 498 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/498 (66%), Positives = 400/498 (80%), Gaps = 2/498 (0%) Query: 12 MAQRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKE 71 M Q + ++TD+ G I+++IYGHFSEHLGRCIYEG+WVGEDSPIPNT+GIRNDV+ AL+E Sbjct: 1 MKQSIKVYTDLEHGKISKHIYGHFSEHLGRCIYEGLWVGEDSPIPNTEGIRNDVLGALQE 60 Query: 72 IKVPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLEC 131 + +PVLRWPGGCFADEYHWKDG+G RE RKRM+NTHWGG VENNHFGTHEFM LC +L Sbjct: 61 LNIPVLRWPGGCFADEYHWKDGVGPRENRKRMVNTHWGGVVENNHFGTHEFMRLCELLGA 120 Query: 132 EPYINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGG 191 EPYI GNVGSGTVQEM EWVEY+TF+G SPMA R +NGREE WA+KYFGVGNENWGCGG Sbjct: 121 EPYICGNVGSGTVQEMQEWVEYMTFDGESPMANWRTENGREEPWALKYFGVGNENWGCGG 180 Query: 192 NMRPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYY 251 +MRPEYYADLYR+YQTYVRNYGDN+I++IA G N DDY+W EVLMREA MD ++LHYY Sbjct: 181 HMRPEYYADLYRRYQTYVRNYGDNKIYKIAGGANVDDYRWTEVLMREAAHLMDGLSLHYY 240 Query: 252 TVPGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGT 311 TVPG WE+KG+A +E+F TL+K+ RM+EL+T+HS IMD+YDPEKRVGLIVDEWGT Sbjct: 241 TVPGDFWENKGSALDDRESEWFKTLKKSFRMDELLTKHSTIMDRYDPEKRVGLIVDEWGT 300 Query: 312 WYDVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGE 371 W+DVEPGTNPGFLYQQN+IRDALVAG+ +IFH+H+DRV MANIAQ +NVLQA++LTEGE Sbjct: 301 WFDVEPGTNPGFLYQQNTIRDALVAGVHFHIFHEHNDRVHMANIAQMVNVLQAMVLTEGE 360 Query: 372 KMLLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSLCN 431 +MLLTPTYHVFNMYKVHQDA L++ D G+Y F E+P V+ SAS EG IHVS+C+ Sbjct: 361 QMLLTPTYHVFNMYKVHQDATRLEVNADVGTYKFGDDELPAVTVSASKDNEGAIHVSVCH 420 Query: 432 LNHASAAVLPLELRGL--AGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGD 489 L+ L L+L GL + A ++G L ++AHN+F P V P P+ + G Sbjct: 421 LDPHRDTDLTLKLNGLNKSLSASSVSGELLTANELNAHNTFERPNEVEPVPYEPASVSGH 480 Query: 490 TVTVELPAMSVTVLEITP 507 + +++PA SV + I P Sbjct: 481 EMELKVPASSVLRVTIRP 498 >gb|AAN05450.1| alpha-L-arabinofuranosidase ArfA [Clostridium cellulovorans] Length = 492 Score = 697 bits (1799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/492 (64%), Positives = 396/492 (80%), Gaps = 2/492 (0%) Query: 14 QRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKEIK 73 ++ ++ +KG I++NIYG+F+EHLGRCIY G WVG+DS IPN GIR DVVEALKEIK Sbjct: 2 NKLTINAQNKKGQIDKNIYGNFAEHLGRCIYGGFWVGKDSSIPNVNGIRTDVVEALKEIK 61 Query: 74 VPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLECEP 133 +PVLRWPGGCFADEYHWKDGIG E R RM+N HWGGTVENNHFGTHEF+ELC ML EP Sbjct: 62 LPVLRWPGGCFADEYHWKDGIGDPEMRPRMVNAHWGGTVENNHFGTHEFLELCDMLGAEP 121 Query: 134 YINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGGNM 193 YINGNVGSGTVQEM EW+EY+TF+G SPMA LRA NGR+ W +KYFGVGNE+WGCGGNM Sbjct: 122 YINGNVGSGTVQEMREWIEYMTFDGESPMANLRAANGRKNPWEIKYFGVGNESWGCGGNM 181 Query: 194 RPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYYTV 253 RPEYYADL+R+Y +YVRN+ N++++IACGPN+ DY W EVLMREA +FMD++TLHYYT Sbjct: 182 RPEYYADLFRRYGSYVRNFSGNQVYKIACGPNSVDYNWTEVLMREAGQFMDALTLHYYTT 241 Query: 254 PGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGTWY 313 WE+KG+AT F+ +F T++ L MEELVT+H IM KYDPEKRV LIVDEWGTW+ Sbjct: 242 GTGVWENKGSATEFEEDLWFRTMKNTLVMEELVTKHDEIMTKYDPEKRVALIVDEWGTWF 301 Query: 314 DVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGEKM 373 +VE GTNPGFLYQQN+IRDALVAG+ LNIF++H DRV+MANIAQTINVLQAVILTEG KM Sbjct: 302 EVEKGTNPGFLYQQNTIRDALVAGINLNIFNEHCDRVKMANIAQTINVLQAVILTEGPKM 361 Query: 374 LLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSLCNLN 433 LLTPTYHVFNMYKVHQDAELL++ + Y F +++P++ +SASV +G IH+S+CNL+ Sbjct: 362 LLTPTYHVFNMYKVHQDAELLEVGKIAQDYIFGEEKVPQIHSSASVDKDGKIHISICNLD 421 Query: 434 HASAAVLPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGDTVTV 493 ++A + ++ G + V+ G+ LA I+ HN+F P+ V P F+ + L+ + +T+ Sbjct: 422 PKASASIECDVEGRDIKTVN--GSVLASEKINDHNTFECPDKVKPSEFSDYSLDNNKITL 479 Query: 494 ELPAMSVTVLEI 505 +PAMSV VLE+ Sbjct: 480 TVPAMSVLVLEL 491 >gb|AAC28125.1| alpha-L-arabinofuranosidase [Clostridium stercorarium] Length = 491 Score = 678 bits (1750), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/493 (63%), Positives = 397/493 (80%), Gaps = 7/493 (1%) Query: 15 RVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKEIKV 74 + V++T RK INR IYGHFSEHLGRCIY G +VGE+SPIPNTKGIRNDVV ALK+I++ Sbjct: 3 KYVINTLNRKSKINREIYGHFSEHLGRCIYGGFYVGENSPIPNTKGIRNDVVNALKKIRI 62 Query: 75 PVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLECEPY 134 PVLRWPGGCFADEYHW DGIG +E RK+MINTHWGG VE + FGT EFMELC LECEPY Sbjct: 63 PVLRWPGGCFADEYHWMDGIGPKEQRKKMINTHWGGVVEAS-FGTDEFMELCRQLECEPY 121 Query: 135 INGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGGNMR 194 INGN+GSGTV+EM +W+EYLTF+G+SPM+ELRAKNGR+E + +KYF +GNENWGCGGNMR Sbjct: 122 INGNLGSGTVKEMQDWIEYLTFDGISPMSELRAKNGRKEPYRLKYFCIGNENWGCGGNMR 181 Query: 195 PEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYYTVP 254 PE+YAD+YR+YQTYVRNYG+N+I++IACGPN+DDY W E +++ A RFMD+I+LHYYTVP Sbjct: 182 PEFYADMYRRYQTYVRNYGNNKIYKIACGPNSDDYNWTETVVKIAGRFMDAISLHYYTVP 241 Query: 255 GATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGTWYD 314 G TW KG++T F E++ TL K L+M+EL+T+H+ I+ +YDP+ R+GL+VDEWGTWYD Sbjct: 242 G-TWHSKGSSTKFTEEEWYITLFKTLKMDELITKHTAIIRRYDPDNRIGLVVDEWGTWYD 300 Query: 315 VEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGEKML 374 VEPGTNPGFLYQQN++RDALVAG+ LNIF+KHSD+V MAN+AQTINVLQAVILTEGEKML Sbjct: 301 VEPGTNPGFLYQQNTMRDALVAGINLNIFNKHSDKVVMANLAQTINVLQAVILTEGEKML 360 Query: 375 LTPTYHVFNMYKVHQDAELLD-LTLDSGSYSFDGK-EIPEVSASASVTAEGVIHVSLCNL 432 LTPTYHVF+MYK HQ AEL++ L L + S K +P + SASV A+G +H++LCNL Sbjct: 361 LTPTYHVFDMYKEHQGAELVEKLLLKTEKKSAQTKYTVPNLHESASVNADGKLHITLCNL 420 Query: 433 NHASAAVLPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGDTVT 492 + + + + E+ G +A ++ G L G D HN+F PE V PQ F + + + Sbjct: 421 SLSESYRVETEIIG--KKAENVTGRILTGKMND-HNTFDAPETVKPQTFDKISVRQNGLV 477 Query: 493 VELPAMSVTVLEI 505 +E+P +SV + + Sbjct: 478 IEIPPVSVVSITV 490 >ref|NP_390729.1| beta-xylosidase; alpha-L-arabinosidase [Bacillus subtilis] sp|P94552|ABF2_BACSU Alpha-L-arabinofuranosidase 2 (Arabinosidase) pir||G69734 beta-xylosidase / alpha-L-arabinosidase xsa - Bacillus subtilis emb|CAA99576.1| arabinosidase [Bacillus subtilis] emb|CAB14811.1| alpha-L-arabinosidase; beta-xylosidase [Bacillus subtilis subsp. subtilis str. 168] Length = 495 Score = 675 bits (1741), Expect = 0.0, Method: Compositional matrix adjust. Identities = 307/492 (62%), Positives = 386/492 (78%), Gaps = 4/492 (0%) Query: 14 QRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKEIK 73 + V+ TDI KGTIN+NIYGHF+EHLGR IYEGIWVG DS IPN GIR DV+EALK++ Sbjct: 4 HQAVIQTDIAKGTINKNIYGHFAEHLGRGIYEGIWVGTDSDIPNINGIRKDVLEALKQLH 63 Query: 74 VPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLECEP 133 +PVLRWPGGCFADEYHW +G+G RK M+NTHWGGT+E+N FGTHEFM LC +LECEP Sbjct: 64 IPVLRWPGGCFADEYHWANGVG---DRKTMLNTHWGGTIESNEFGTHEFMMLCELLECEP 120 Query: 134 YINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGGNM 193 YI GNVGSGTVQEMSEW+EY+TF +PM++ R +NGREE W +KYFGVGNENWGCGGNM Sbjct: 121 YICGNVGSGTVQEMSEWIEYMTFEEGTPMSDWRKQNGREEPWKLKYFGVGNENWGCGGNM 180 Query: 194 RPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYYTV 253 PEYYADLYR++QTYVRNY N I++IA G N DD+ W +VLM++A MD ++LHYYT+ Sbjct: 181 HPEYYADLYRRFQTYVRNYSGNDIYKIAGGANVDDFNWTDVLMKKAAGLMDGLSLHYYTI 240 Query: 254 PGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGTWY 313 PG W+ KG+AT F E+F T++KA ++EL+ +H IMD+YDPE+RVGLI+DEWGTW+ Sbjct: 241 PGDFWKGKGSATEFTEDEWFITMKKAKYIDELIQKHGTIMDRYDPEQRVGLIIDEWGTWF 300 Query: 314 DVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGEKM 373 D EPGTNPGFLYQQN+IRDALVA +IFH+H RV+MANIAQT+NVLQA+ILTEGE+M Sbjct: 301 DPEPGTNPGFLYQQNTIRDALVAASHFHIFHQHCRRVQMANIAQTVNVLQAMILTEGERM 360 Query: 374 LLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSLCNLN 433 LLTPTYHVFNM+KVHQDA LL S Y ++G+ P++S SAS AEG I++++CN++ Sbjct: 361 LLTPTYHVFNMFKVHQDASLLATETMSADYEWNGETFPQISISASKQAEGDINITICNID 420 Query: 434 HASAAVLPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGDTVTV 493 H + A +ELRGL +A D +G L ++AHN+F +P V P+ F + L + + V Sbjct: 421 HQNKAEAEIELRGLH-KAADHSGVILTAEKMNAHNTFDDPHHVKPESFRQYTLSKNKLKV 479 Query: 494 ELPAMSVTVLEI 505 +LP MSV +L + Sbjct: 480 KLPPMSVVLLTL 491 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 810,032,335 Number of extensions: 36132671 Number of successful extensions: 75587 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 75421 Number of HSP's successfully gapped: 72 Length of query: 509 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 379 Effective length of database: 404,274,708 Effective search space: 153220114332 Effective search space used: 153220114332 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)