BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0054.p (847 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44683 precorrin methylase (EC 2.1.1.-) [imported] - Bacill... 431 e-119 ref|ZP_00043656.1| COG1010: Precorrin-3B methylase [Magnetococcu... 302 2e-80 ref|NP_714701.1| precorrin methylase [Leptospira interrogans ser... 296 1e-78 gb|EAJ15730.1| unknown [environmental sequence] 285 2e-75 gb|EAK39294.1| unknown [environmental sequence] 282 2e-74 >pir||T44683 precorrin methylase (EC 2.1.1.-) [imported] - Bacillus megaterium emb|CAA04307.1| precorrin-3 methylase [Bacillus megaterium] Length = 540 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 195/273 (71%), Positives = 239/273 (87%) Query: 4 QGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVS 63 +GKLL+IGFGPG EHIT RA A++ES+ +IGY TYV+LI+ LL Q+I+ TGMTEEVS Sbjct: 2 KGKLLVIGFGPGSFEHITQRAREAIQESDMIIGYKTYVELIQGLLTNQQIISTGMTEEVS 61 Query: 64 RAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQS 123 RAQEAV++AEAG+TVAVISSGDAGVYGMAGLVYEVLIE+GW + TGVE+EVIPG+SAI S Sbjct: 62 RAQEAVKQAEAGKTVAVISSGDAGVYGMAGLVYEVLIEKGWKKETGVELEVIPGISAINS 121 Query: 124 CSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEAR 183 C+SLLGAP+MHD+CTISLSDHLTPWE I R+EAA ADFV+AFYNP+SG+RTRQI EA+ Sbjct: 122 CASLLGAPVMHDACTISLSDHLTPWELIEKRIEAAAQADFVVAFYNPKSGRRTRQIVEAQ 181 Query: 184 QILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLMV 243 +ILL+YR P TPVG+VKSAYRDR++ ++T L+DML+HEIGML+TV+VGNS+T YD LM+ Sbjct: 182 RILLKYRSPDTPVGLVKSAYRDREEVVMTNLKDMLNHEIGMLTTVVVGNSSTFFYDDLMI 241 Query: 244 TPRGYERKYSLGADTQALKPHERLRTSSEPWSL 276 TPRGY+RKY+L Q L+PH+RLR +EPW+L Sbjct: 242 TPRGYQRKYTLNQTEQPLRPHQRLRKEAEPWAL 274 Score = 109 bits (273), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 51/145 (35%), Positives = 78/145 (53%) Query: 279 LNPTDNVVKVKTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLE 338 L+P +V +K C FC K A L ++L G +P+EL++ + GCGMAC AV E Sbjct: 390 LSPVGDVFTIKACDFCDGEKSDAIPHTEELQKRLGGMDMPKELKLGINGCGMACYGAVQE 449 Query: 339 DIGLVYSKGSYELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERF 398 DIG+VY KG+++L+LG K GR H+G V G++ +Y +G +ERF Sbjct: 450 DIGIVYRKGAFDLFLGAKTVGRNAHSGQIVAEGIAPDDIVEIVENIIHEYKEKGHPNERF 509 Query: 399 YAFFERMGSGISAHYPQAESALRCE 423 + FF+R+ + Y ++ E Sbjct: 510 HKFFKRVKNVYGFDYQDITPKIKVE 534 >ref|ZP_00043656.1| COG1010: Precorrin-3B methylase [Magnetococcus sp. MC-1] Length = 423 Score = 302 bits (774), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 141/250 (56%), Positives = 187/250 (74%) Query: 3 KQGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEV 62 K GK+ ++G GPG H+T RA A+ +++ VIGYTTY+ L++PLL Q++V GM EE+ Sbjct: 2 KAGKIYLVGLGPGAEAHMTARAREAIADADVVIGYTTYIRLVQPLLSHQQVVKKGMAEEL 61 Query: 63 SRAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQ 122 R +EA+ A+ G+ VA+ISSGD G+YGMAG YEVL + GW +G+ VEV+PG +A+ Sbjct: 62 DRCEEAIVFAKQGKKVALISSGDVGIYGMAGPTYEVLFKSGWSPDSGIAVEVVPGCTALS 121 Query: 123 SCSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEA 182 SC+SL+GAP+ HD C+ISLSD LTPW IA R+ AA +DFVIA YNP+SG+RT QI A Sbjct: 122 SCASLVGAPLGHDFCSISLSDLLTPWPVIAKRLNAAACSDFVIALYNPKSGRRTEQIVAA 181 Query: 183 RQILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLM 242 ++ILL +R TPV IVKSAYR RQ ++TTL+ M+D EIGMLSTV++GN T V +G+M Sbjct: 182 QKILLHHRSGKTPVAIVKSAYRPRQSVVMTTLKKMVDEEIGMLSTVLIGNGETFVKEGIM 241 Query: 243 VTPRGYERKY 252 +TPRGY KY Sbjct: 242 ITPRGYADKY 251 >ref|NP_714701.1| precorrin methylase [Leptospira interrogans serovar lai str. 56601] ref|YP_003518.1| precorrin-3 C-17 methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] gb|AAN51716.1| precorrin methylase [Leptospira interrogans serovar lai str. 56601] gb|AAS72155.1| precorrin-3 C-17 methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 497 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 148/294 (50%), Positives = 204/294 (69%), Gaps = 9/294 (3%) Query: 3 KQGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEV 62 K+G+L I+G GPG EHIT A A++E++ VIGY TY++L+K L +++ TGMTEE+ Sbjct: 2 KKGRLNIVGIGPGNDEHITPAAFHAIQEADLVIGYVTYINLVKHQLVGKQVTQTGMTEEI 61 Query: 63 SRAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQ 122 RAQEAV A+ G+ V ++SSGDAGVYGMAGLV+EVL + GW + E+++IPG++A Sbjct: 62 DRAQEAVNTAKEGKIVTLVSSGDAGVYGMAGLVFEVLRKMGWKKEESPEIKIIPGITADS 121 Query: 123 SCSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEA 182 SC+SL+GAPI+HD+ ISLSD LTPW I R++ A DFVI YNP SG+R RQI +A Sbjct: 122 SCASLIGAPIIHDTARISLSDLLTPWSVIENRLKCAAQGDFVITLYNPASGRRQRQIVKA 181 Query: 183 RQILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLM 242 +I+ R+R TPV ++KSAYR +Q ++ L + L++EIGM +TVIVG+S T VY+G M Sbjct: 182 SKIIKRHRSGDTPVALIKSAYRKQQNVFLSDLDNFLNYEIGMNTTVIVGSSNTFVYEGFM 241 Query: 243 VTPRGYERKYSLGADTQALKPHER----LRTSSEPWSLAALNPTD---NVVKVK 289 +TPRGY KYSL + +LK ++ LRT + S L T NV K++ Sbjct: 242 ITPRGYTNKYSL--EDASLKEGQKKGFSLRTIEDLKSNTNLEDTSLNLNVTKIR 293 >gb|EAJ15730.1| unknown [environmental sequence] Length = 261 Score = 285 bits (730), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 130/253 (51%), Positives = 186/253 (73%), Gaps = 2/253 (0%) Query: 4 QGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVS 63 +GKL I+G GPG +H+T RA +EES+ ++GYTTYV+L++ L++ +EI MT+EV Sbjct: 2 EGKLYIVGVGPGHHDHMTFRAKQVIEESDTIVGYTTYVNLVEDLIQGKEIHSYAMTQEVE 61 Query: 64 RAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQS 123 RA + + A+ G+ V+++SSGD G+YGMAGL++EVL E GWD +G+ VE+IPGVSA+ S Sbjct: 62 RAHQCIDLAKEGKIVSLVSSGDPGIYGMAGLIFEVLAESGWDPKSGLHVELIPGVSALNS 121 Query: 124 CSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEAR 183 C+S++G+P+M D S+SD + PWE I RVEAA DFV+ YNP S KR Q+++ R Sbjct: 122 CASIIGSPLMTDFAVCSMSDLMVPWEIIVKRVEAAAQGDFVLVIYNPASKKRVHQLQDTR 181 Query: 184 QILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDH--EIGMLSTVIVGNSATMVYDGL 241 +ILL+YR P+TPV I+K AYR+ + I+T L+ M ++ E+GM STVIVGNS+T + L Sbjct: 182 KILLKYRKPTTPVAIIKGAYRESEAVIITDLEHMENYAEELGMTSTVIVGNSSTYNFKDL 241 Query: 242 MVTPRGYERKYSL 254 M+ PRGY+ KY+L Sbjct: 242 MINPRGYKSKYNL 254 >gb|EAK39294.1| unknown [environmental sequence] Length = 262 Score = 282 bits (722), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 128/252 (50%), Positives = 183/252 (72%), Gaps = 2/252 (0%) Query: 5 GKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVSR 64 GKL I+G GPG +H+T RA +EES+ ++GY TYV+L+K L+ +++ MT+EV R Sbjct: 3 GKLYIVGVGPGHHDHMTFRAKQVIEESDTIVGYETYVNLVKDLINGKDVYRYAMTQEVER 62 Query: 65 AQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQSC 124 A + + A+ G+ V+++SSGD G+YGMAGL++EVL E GWD +G+ VE+IPGVSA+ SC Sbjct: 63 AHQCIDLAKEGKIVSLVSSGDPGIYGMAGLIFEVLAESGWDPKSGLHVELIPGVSALNSC 122 Query: 125 SSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEARQ 184 +S++G+P+M D S+SD L PWE I RVEAA DFVI YNP S KR Q+++ R+ Sbjct: 123 ASIIGSPLMTDFAVCSMSDLLVPWEIIVKRVEAAAQGDFVIVIYNPSSKKRIHQLQDTRK 182 Query: 185 ILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDH--EIGMLSTVIVGNSATMVYDGLM 242 ILL+YR P TPV I+K AYR+ + ++T L+ M ++ ++GM+STVI+GNSAT + LM Sbjct: 183 ILLKYRKPETPVAIIKGAYRESETVVMTDLEHMENYADKLGMISTVIIGNSATYNFKDLM 242 Query: 243 VTPRGYERKYSL 254 + PRGY+ KY+L Sbjct: 243 INPRGYKSKYNL 254 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,110,781,191 Number of extensions: 43251718 Number of successful extensions: 128763 Number of sequences better than 1.0e-05: 362 Number of HSP's gapped: 128615 Number of HSP's successfully gapped: 415 Length of query: 847 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 713 Effective length of database: 393,414,312 Effective search space: 280504404456 Effective search space used: 280504404456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)