BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0053.p (230 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44685 cobalamin biosynthesis J protein homologcobalamin bi... 214 2e-54 ref|ZP_00180570.1| hypothetical protein Mther147701 [Moorella th... 144 2e-33 ref|ZP_00100023.1| COG2099: Precorrin-6x reductase [Desulfitobac... 143 4e-33 ref|NP_781404.1| precorrin-6X reductase [Clostridium tetani E88]... 131 1e-29 ref|NP_930224.1| Precorrin-6X reductase [Photorhabdus luminescen... 122 5e-27 >pir||T44685 cobalamin biosynthesis J protein homologcobalamin biosynthesis J protein homolog [imported] - Bacillus megaterium emb|CAA04309.1| precorrin-6x reductase [Bacillus megaterium] Length = 255 Score = 214 bits (544), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 110/224 (49%), Positives = 155/224 (69%), Gaps = 4/224 (1%) Query: 1 VTPSAAERLEAAGITTRVGRLDQQGMIACLRGLDCSAIIDASHPFAQEAHDHAMGAAEAL 60 VT +AA L+ A + ++GRL ++ M + AI+DASHPFA+EA +A+GAA Sbjct: 30 VTDNAAIELQRAEVKVKIGRLTKEDMTDFINEHGVKAIVDASHPFAEEASKNAIGAAAET 89 Query: 61 ELPYLRYERRSLKYEPHPRLLVVSSYGEAARRAKQLKGSVMLTTGGKTLEIFAEALLGDP 120 +PY+RYER S + + + +VS+Y EAA A + KG +MLTTG KTL++F E LL Sbjct: 90 AIPYIRYERASQAF-TYDNMTMVSTYEEAAEVAAEKKGVIMLTTGSKTLQVFTEKLLPLS 148 Query: 121 AIRLTVRLLPCLENMQKCHLLGIEQRNIIALQGPFSRELNEALYRQYGTQVMVTKESGAE 180 +RL R+LP L+NM+KC LG+ Q+NIIA+QGPF++E + ALY+QYG VMVTKESG Sbjct: 149 DVRLVARMLPRLDNMEKCQQLGLPQKNIIAIQGPFTKEFDRALYKQYGVTVMVTKESGKV 208 Query: 181 GSLDEKVHAALDMGIYVILIARPQAFYKGHGRVYESFEEIVSAV 224 GS+D+KV AA ++G+ +I+I RP+ Y G VY +FEE+V A+ Sbjct: 209 GSVDKKVEAAKELGLDIIMIGRPKIEY---GTVYSTFEEVVHAL 249 >ref|ZP_00180570.1| hypothetical protein Mther147701 [Moorella thermoacetica ATCC 39073] Length = 254 Score = 144 bits (362), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 81/207 (39%), Positives = 124/207 (59%), Gaps = 9/207 (4%) Query: 1 VTPSAAERLEAAGIT-TRVGRLDQQGMIACLRGLDCSAIIDASHPFAQEAHDHAMGAAEA 59 VTP A+ AG R G L+ + + A L A+IDA+HP+A A Sbjct: 30 VTPYGAKLAREAGADRVREGALEGEDLAALLGTGKIEAVIDATHPYATTITSRAREICRV 89 Query: 60 LELPYLRYERRSLKYEPHPRLLVVSSYGEAARRAKQLKGSVMLTTGGKTLEIFAEALLGD 119 +PY RY+R +++ HP LL+ + + +A A + G++MLT G + LE+F A Sbjct: 90 TGIPYFRYQRPAVELPAHPNLLIAADWTQAVELASRY-GTIMLTIGTRHLELFTAA---- 144 Query: 120 PAI---RLTVRLLPCLENMQKCHLLGIEQRNIIALQGPFSRELNEALYRQYGTQVMVTKE 176 PA+ R+ R+LP + +++KC LG+ +IIA+QGP S ELN ALYRQ+G + +VTK+ Sbjct: 145 PAMAGKRIIARVLPEVTSLEKCRQLGLWPGDIIAIQGPCSYELNRALYRQFGVEAVVTKD 204 Query: 177 SGAEGSLDEKVHAALDMGIYVILIARP 203 SG+ G ++ K+ AALD+G+ VI++ RP Sbjct: 205 SGSTGGVESKIQAALDLGLQVIVLRRP 231 >ref|ZP_00100023.1| COG2099: Precorrin-6x reductase [Desulfitobacterium hafniense] Length = 261 Score = 143 bits (360), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 83/212 (39%), Positives = 118/212 (55%), Gaps = 7/212 (3%) Query: 1 VTPSAAERLEAAGITTRVGRLDQQGMIACLRGLDCSAIIDASHPFAQEAHDHAMGAAEAL 60 +T AE E G+ T G LDQ ++ L+ A+IDA+HP+AQ + A A Sbjct: 31 LTEYGAELAERQGLRTCSGALDQDSLMKILQEQAIHALIDATHPYAQNIRNLARCVAGEC 90 Query: 61 ELPYLRYERRSLKYEPHPRLLVVSSYGEAARRAKQLKGSVMLTTGGKTLEIFAEALLGDP 120 LPY R+ER S Y HP L ++ AAR A +L + L+TG K L + E+ P Sbjct: 91 NLPYFRWERPSGAYPVHPLLYFTATIEGAARLAARLGKRIFLSTGSKNLRDWMES----P 146 Query: 121 AIR---LTVRLLPCLENMQKCHLLGIEQRNIIALQGPFSRELNEALYRQYGTQVMVTKES 177 ++ L VR+LP + + +C G++ IIA+QGPFS+ NE L+ Q V++TK+S Sbjct: 147 KLKDCQLYVRVLPTSQVLSQCEEAGLKPYQIIAMQGPFSQRFNEGLWEQLNIDVVITKDS 206 Query: 178 GAEGSLDEKVHAALDMGIYVILIARPQAFYKG 209 GA G DEKV A L +GI +I++ RPQ G Sbjct: 207 GAVGGTDEKVQACLQLGIPIIVLERPQETQAG 238 >ref|NP_781404.1| precorrin-6X reductase [Clostridium tetani E88] gb|AAO35341.1| precorrin-6X reductase [Clostridium tetani E88] Length = 256 Score = 131 bits (330), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 69/203 (33%), Positives = 117/203 (57%), Gaps = 8/203 (3%) Query: 21 LDQQGMIACLRGLDCSAIIDASHPFAQEAHDHAMGAAEALELPYLRYERRSL--KYEPHP 78 LD++ MI + +IDASHP+A E +AM ++ Y+R+ER + K+ Sbjct: 51 LDRENMIKLFKEKKVEVLIDASHPYALEVSKNAMEVCNHTDIEYIRFEREKVIEKFLEKE 110 Query: 79 RLLVVSSYGEAARRAKQLKGSVMLTTGGKTLEIFAEALLGDPAIRLTVRLLPCLENMQKC 138 ++LVV +Y + + K +KG+++ TTG + +E L + R+ R+LP L+ +++C Sbjct: 111 KVLVVENYEDLYSKLKDIKGNILNTTGSRNIEKIIRLSLEN---RIIHRVLPSLKVIEEC 167 Query: 139 HLLGIEQRNIIALQGPFSRELNEALYRQYGTQVMVTKESGAEGSLDEKVHAALDMGIYVI 198 LG+E NIIA++GP S +LN A R+Y + ++ K+SG EG +EK+ AA+D I I Sbjct: 168 FHLGLELHNIIAVKGPISYDLNRAFIREYDAKAILLKDSGKEGGTEEKIKAAIDENICAI 227 Query: 199 LIARPQAFYKGHGRVYESFEEIV 221 ++ R YK + + EE++ Sbjct: 228 VVDRKSLDYKNK---FNNKEEVI 247 >ref|NP_930224.1| Precorrin-6X reductase [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE15364.1| Precorrin-6X reductase [Photorhabdus luminescens subsp. laumondii TTO1] Length = 261 Score = 122 bits (307), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 77/214 (35%), Positives = 112/214 (52%), Gaps = 5/214 (2%) Query: 18 VGRLDQQGMIACLRGLDCSAIIDASHPFAQEAHDHAMGAAEALELPYLRYERRS-LKYEP 76 VGR+D + M+ +IDASHP+A + + A L L RY+R S + Sbjct: 51 VGRMDAEAMVDYFDQHKVRWVIDASHPYADALSQNVLTACRRLSLRLTRYQRPSEIDLLS 110 Query: 77 HPRLLVVSSYGEAARRAKQLKGSVMLTTGGKTLEIFAEALLGDPAIRLTVRLLPCLENMQ 136 HP L V+S A A+ L ++LTTG K LE++ L P L VR+LP E + Sbjct: 111 HPLLHKVNSIEAACDVARTLGQRILLTTGSKQLELYQRLL---PGKTLLVRVLPTAEVLI 167 Query: 137 KCHLLGIEQRNIIALQGPFSRELNEALYRQYGTQVMVTKESGAEGSLDEKVHAALDMGIY 196 +C LG+ +IIAL+GPF+ E N A+Y V++TKESGAEG +KV L + I Sbjct: 168 QCEALGLGVDHIIALRGPFTAEFNHAVYEFCHPDVVITKESGAEGGYQQKVAPCLALNIP 227 Query: 197 VILIARPQAFYKG-HGRVYESFEEIVSAVTAFNK 229 I++ RP +G G S +E V + ++ + Sbjct: 228 CIVVCRPTQTLQGDEGEQISSLDEFVQHLASWQE 261 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 306,770,663 Number of extensions: 11209412 Number of successful extensions: 32550 Number of sequences better than 1.0e-05: 60 Number of HSP's gapped: 32495 Number of HSP's successfully gapped: 60 Length of query: 230 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 109 Effective length of database: 428,710,599 Effective search space: 46729455291 Effective search space used: 46729455291 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)