BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0051.p (297 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44687 cobalamin biosynthesis protein [imported] - Bacillus... 335 6e-91 ref|NP_714706.1| cobalamin biosynthetic protein [Leptospira inte... 196 4e-49 ref|YP_003523.1| cobalamin biosynthesis protein [Leptospira inte... 196 5e-49 ref|ZP_00126067.1| COG1903: Cobalamin biosynthesis protein CbiD ... 195 1e-48 ref|ZP_00000571.1| COG1903: Cobalamin biosynthesis protein CbiD ... 194 1e-48 >pir||T44687 cobalamin biosynthesis protein [imported] - Bacillus megaterium emb|CAA04311.1| CbiD protein [Bacillus megaterium] Length = 367 Score = 335 bits (860), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 167/293 (56%), Positives = 207/293 (70%) Query: 1 VKDAGDDPDATHQARIEAAVSWRDSPGVEIDGGKGVGRVTKPGLPVAVGEAAINPVPRRM 60 +KDAGDDPDATH A I + VSW + + IDGG GVGRVTKPGLPV VGEAAINPVPR+M Sbjct: 70 IKDAGDDPDATHGALIISEVSWCNGVDIIIDGGVGVGRVTKPGLPVPVGEAAINPVPRKM 129 Query: 61 ILEAVSEVLEEHGAARGVRVVISVPEGEAIALKTLNPRXXXXXXXXXXXTRGVVTPFSTE 120 + E ++L E+ +GV+VVISVPEGE +A KTLN R TRG+V PFST Sbjct: 130 LKETAQQLLAEYNIQKGVKVVISVPEGEEMAKKTLNARLGILGGISILGTRGIVVPFSTA 189 Query: 121 AYKXXXXXXXXXXXXMGNKQIILTTGGSSEKYAMAMFAELPEEAFIQMGEYVGFSLEHAK 180 AYK + +++TTGG SEKY M F ELPEEAFIQMG++VGF+L+ K Sbjct: 190 AYKASIVQAISVAKASNCEHVVITTGGRSEKYGMKQFPELPEEAFIQMGDFVGFTLKQCK 249 Query: 181 AFGVRKITLVGMAGKFSKVAQGAMLIHSKNAPVDFSFLAKIAAESGADNELVGEIAAANT 240 G++K++LVGM GKFSKVAQG M++HSK+AP+DF+FLAK A+ESGA ELV EI ANT Sbjct: 250 KQGMKKVSLVGMMGKFSKVAQGVMMVHSKSAPIDFNFLAKAASESGASAELVEEIKGANT 309 Query: 241 ASQVTDLMTAAGNGVFFEQLCLHACRHCLKHMNGGMTVEMVLVTMKGRVLGRA 293 ASQV DLMT +G+ FFE+LC + C LK + G+ V+ L T+KG LG+A Sbjct: 310 ASQVGDLMTQSGHHQFFEKLCEYCCLSALKEVGDGIDVDTSLYTLKGDFLGQA 362 >ref|NP_714706.1| cobalamin biosynthetic protein [Leptospira interrogans serovar lai str. 56601] gb|AAN51721.1| cobalamin biosynthetic protein [Leptospira interrogans serovar lai str. 56601] Length = 367 Score = 196 bits (499), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 108/294 (36%), Positives = 159/294 (54%), Gaps = 1/294 (0%) Query: 1 VKDAGDDPDATHQARIEAAVSWRDSPGVEIDGGKGVGRVTKPGLPVAVGEAAINPVPRRM 60 VKDAGDDPD TH A + A V +++ GG GV VTK GL + +G++AINP+PR+ Sbjct: 65 VKDAGDDPDCTHGAELTARVRLTKENEIKLKGGDGVAVVTKAGLGLEIGQSAINPIPRKN 124 Query: 61 ILEAVSEVLEEHGAARGVRVVISVPEGEAIALKTLNPRXXXXXXXXXXXTRGVVTPFSTE 120 I E + E L+ + G V ISVP G+ +A KT+N R T G+V P+ST Sbjct: 125 IKEMILEELQ-GSSFNGAEVEISVPGGQEMAKKTMNERLGLIGGISILGTTGIVKPYSTA 183 Query: 121 AYKXXXXXXXXXXXXMGNKQIILTTGGSSEKYAMAMFAELPEEAFIQMGEYVGFSLEHAK 180 A+K G I+LTTGG SEK+AM + L E +FIQ+G+++G ++ + Sbjct: 184 AFKASVIQAIQMAKEYGIDTIVLTTGGKSEKFAMDLLPNLNELSFIQVGDFIGTGIKTSV 243 Query: 181 AFGVRKITLVGMAGKFSKVAQGAMLIHSKNAPVDFSFLAKIAAESGADNELVGEIAAANT 240 +R +VGM GK SK+A G M+ H + V+ + L+ IA G E+ EI ANT Sbjct: 244 KESIRHTIIVGMIGKLSKMADGVMMTHRGGSSVNTTMLSTIARSIGVPEEIAIEIQNANT 303 Query: 241 ASQVTDLMTAAGNGVFFEQLCLHACRHCLKHMNGGMTVEMVLVTMKGRVLGRAE 294 A V ++ +G + ++C C KH + + +V G++LG+ E Sbjct: 304 ARHVLEICKVSGYKIITTKICEIVAEKCSKHAGTNIMISCYMVDFDGKLLGKCE 357 >ref|YP_003523.1| cobalamin biosynthesis protein [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] gb|AAS72160.1| cobalamin biosynthesis protein [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 367 Score = 196 bits (499), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 108/294 (36%), Positives = 159/294 (54%), Gaps = 1/294 (0%) Query: 1 VKDAGDDPDATHQARIEAAVSWRDSPGVEIDGGKGVGRVTKPGLPVAVGEAAINPVPRRM 60 VKDAGDDPD TH A + A V +++ GG GV VTK GL + +G++AINP+PR+ Sbjct: 65 VKDAGDDPDCTHGAELTARVRLTKENEIKLKGGDGVAVVTKAGLGLEIGQSAINPIPRKN 124 Query: 61 ILEAVSEVLEEHGAARGVRVVISVPEGEAIALKTLNPRXXXXXXXXXXXTRGVVTPFSTE 120 I E + E L+ + G V ISVP G+ +A KT+N R T G+V P+ST Sbjct: 125 IKEMILEELQ-GSSFNGAEVEISVPGGQEMAKKTMNERLGLIGGISILGTTGIVKPYSTA 183 Query: 121 AYKXXXXXXXXXXXXMGNKQIILTTGGSSEKYAMAMFAELPEEAFIQMGEYVGFSLEHAK 180 A+K G I+LTTGG SEK+AM + L E +FIQ+G+++G ++ + Sbjct: 184 AFKASVIQAIQMAKEYGIDTIVLTTGGKSEKFAMDLLPNLNELSFIQVGDFIGTGIKTSV 243 Query: 181 AFGVRKITLVGMAGKFSKVAQGAMLIHSKNAPVDFSFLAKIAAESGADNELVGEIAAANT 240 +R +VGM GK SK+A G M+ H + V+ + L+ IA G E+ EI ANT Sbjct: 244 KESIRHTIIVGMIGKLSKMADGVMMTHRGGSSVNTTMLSTIARSIGVPEEIAIEIQNANT 303 Query: 241 ASQVTDLMTAAGNGVFFEQLCLHACRHCLKHMNGGMTVEMVLVTMKGRVLGRAE 294 A V ++ +G + ++C C KH + + +V G++LG+ E Sbjct: 304 ARHVLEICKVSGYKIITTKICEIVAEKCSKHAGTNIMISCYMVDFDGKLLGKCE 357 >ref|ZP_00126067.1| COG1903: Cobalamin biosynthesis protein CbiD [Pseudomonas syringae pv. syringae B728a] Length = 386 Score = 195 bits (495), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 117/295 (39%), Positives = 167/295 (56%), Gaps = 2/295 (0%) Query: 1 VKDAGDDPDATHQARIEAAVSWRDSPGVEIDGGKGVGRVTKPGLPVAVGEAAINPVPRRM 60 +KDAGDDPD TH A + A V + + GV G GVG VT+PGL +AVGE AINPVPRRM Sbjct: 87 LKDAGDDPDVTHGALVFARVRLQAAAGVRFLAGAGVGTVTRPGLVLAVGEPAINPVPRRM 146 Query: 61 ILEAVSEVLEEHGAARGVRVVISVPEGEAIALKTLNPRXXXXXXXXXXXTRGVVTPFSTE 120 + E + ++ EE G + G V I V GEA+ALKT+NPR T G+V PFS Sbjct: 147 MTEHLLQLAEEQGYSGGFEVTIGVEGGEALALKTMNPRLGILGGLSILGTSGIVRPFSCA 206 Query: 121 AYKXXXXXXXXXXXXMGNKQIILTTGGSSEKYAMAMFAELPEEAFIQMGEYVGFSLEHAK 180 AY G + I TG +SE M ++P+ A I+MG++VG L+H + Sbjct: 207 AYIASIHQGIDVATTNGYRHIAACTGNASED-TMRRVYKIPDIALIEMGDFVGAVLKHLR 265 Query: 181 AFGVRKITLVGMAGKFSKVAQGAMLIHSKNAPVDFSFLAKIAAESGADNELVGEIAAANT 240 V K+++ G GK SK+A G M +HS+++ +D LA+ AA+ GAD L +I AANT Sbjct: 266 RVSVDKLSVCGGFGKISKLAAGHMDLHSRHSSIDLPQLARWAADVGADAGLQQQILAANT 325 Query: 241 ASQVTDLMTAAGNGVFFEQLCLHACRHCLKHMNGGMTVEMVLVTMKGRVLGRAEI 295 + Q M+AA +++C HA + + VE+ + +G ++G+A + Sbjct: 326 SQQAL-AMSAAAGVPLGDEVCRHALNFARSIVPASVQVEVFAIDRQGGLVGQAGV 379 >ref|ZP_00000571.1| COG1903: Cobalamin biosynthesis protein CbiD [Ferroplasma acidarmanus] Length = 361 Score = 194 bits (494), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 100/282 (35%), Positives = 156/282 (55%), Gaps = 2/282 (0%) Query: 2 KDAGDDPDATHQARIEAAVSWRDSPGVEIDGGKGVGRVTKPGLPVAVGEAAINPVPRRMI 61 KD GDDPD T I + V + + G+EI GG+GVG VTK GLP+ G AINPVP +M+ Sbjct: 78 KDGGDDPDVTTGLYIYSRVEYSEKSGIEITGGEGVGVVTKEGLPIKPGNPAINPVPLKML 137 Query: 62 LEAVSEVLEEHGAARGVRVVISVPEGEAIALKTLNPRXXXXXXXXXXXTRGVVTPFSTEA 121 A +EVL+ + RG+++ ISVP G +A KT NP+ TRG+V PFS + Sbjct: 138 RAAATEVLDSYEVHRGLKITISVPGGAEVATKTCNPKLGIIGGISILGTRGIVIPFSDSS 197 Query: 122 YKXXXXXXXXXXXXMGNKQIILTTGGSSEKYAMAMFAELPEEAFIQMGEYVGFSLEHAKA 181 +K M ++ +TGG S+ +F + EE FI++G+++GFS++ A Sbjct: 198 WKASIVLGIRIASRMDMDTLVFSTGGRSDTAVHKIFPDFKEEQFIEIGDFLGFSVKRAVD 257 Query: 182 FGVRKITLVGMAGKFSKVAQGAMLIHSKNAPVDFSFLAKIAAESGADNELVGEIAAANTA 241 G+R + + GM GK SK+A M +HS + V+F FLA I + G +E++ I ANT Sbjct: 258 TGIRNLVIAGMPGKISKLADNNMDLHSSKSSVNFDFLASIGKKIGYPDEIISRIRHANTV 317 Query: 242 SQVTDLMTAAGNGVFFEQLCLHACRHCLKHMNGGMTVEMVLV 283 V +++ + +F + L + + K + V++ ++ Sbjct: 318 LNVMEIINY--DSIFLDTLKERSIENINKITGNKIKVDIEII 357 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 381,586,913 Number of extensions: 14051670 Number of successful extensions: 39018 Number of sequences better than 1.0e-05: 79 Number of HSP's gapped: 38890 Number of HSP's successfully gapped: 79 Length of query: 297 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 172 Effective length of database: 417,850,203 Effective search space: 71870234916 Effective search space used: 71870234916 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)