BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0049.p (394 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44688 precorrin-6Y methylase [imported] - Bacillus megater... 332 6e-90 ref|YP_003521.1| precorrin-6y methylase [Leptospira interrogans ... 295 2e-78 ref|NP_714704.1| precorrin-6Y methylase [Leptospira interrogans ... 295 2e-78 ref|ZP_00073499.1| COG2242: Precorrin-6B methylase 2 [Trichodesm... 254 3e-66 ref|ZP_00017166.1| hypothetical protein [Chloroflexus aurantiacus] 243 4e-63 >pir||T44688 precorrin-6Y methylase [imported] - Bacillus megaterium emb|CAA04312.1| CbiET protein [Bacillus megaterium] Length = 398 Score = 332 bits (852), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 172/365 (47%), Positives = 234/365 (64%), Gaps = 17/365 (4%) Query: 26 VQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLASGDPLFFGI 85 + S+VL+GG+R L FF +QGEK+ ++ GL + ++L+ +E AVVLASGDPLF+GI Sbjct: 24 IYESDVLIGGKRHLDFFQDFQGEKVAIEGGLSSLVERLKN--EEGNAVVLASGDPLFYGI 81 Query: 86 AGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRIDGKHKIA 145 YL + VE+ P+ SS+QLAF+RL + WQDA S+HGR I+GLAQRIDG K+A Sbjct: 82 GSYLSTKL---DVEIYPYLSSIQLAFSRLKERWQDAYFTSVHGRSIKGLAQRIDGYKKVA 138 Query: 146 LLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNFAPLNVVIL 205 +LTDE N+P + YL FGMTEY+ FV E LGG E C+ L+E F+PLNVVIL Sbjct: 139 ILTDEQNSPTALANYLLSFGMTEYKMFVAENLGGETERCQLLSLEEAANQFFSPLNVVIL 198 Query: 206 RWKPGSGTAAVRRGFAYP----DEAFRQRRPEKGLITKREVRALVLSELNLFEEAVVWDI 261 + V +P D+ F QR+P+KGLITK+E+R L +S L L ++VVWDI Sbjct: 199 K--------QVEESPVWPLGIEDDEFIQRKPDKGLITKKEIRTLSISALQLKRDSVVWDI 250 Query: 262 GSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLERLPD 321 G+ +G+VA E A+IAR G+++A+EK+ +L N N +FR D + KAP+GL D Sbjct: 251 GTCTGSVAIEAAKIAREGQIFAVEKNEADLENCRENLAKFRVDAHTVHGKAPEGLNEFAD 310 Query: 322 PDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEVTQLX 381 PDAVFIGG+ G ++ I+ C RL GRIV+ A+TIE L ++A K G T VT Sbjct: 311 PDAVFIGGTAGGMETILDVCCSRLNSGGRIVLNAVTIENLAEAMKAFKERGFETAVTLAQ 370 Query: 382 XXKGK 386 + K Sbjct: 371 ISRSK 375 >ref|YP_003521.1| precorrin-6y methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] gb|AAS72158.1| precorrin-6y methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 411 Score = 295 bits (754), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 159/377 (42%), Positives = 234/377 (62%), Gaps = 13/377 (3%) Query: 18 LTPDSLSKVQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLAS 77 L+ + + V ++++L GG+R L FF + GEKIV K+ L ++ E+ E VLAS Sbjct: 17 LSSIAANAVAKAQILAGGKRHLDFFPQFSGEKIVFKDDLTQTIKRIAELSLEHTICVLAS 76 Query: 78 GDPLFFGIAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQR 137 GDPLFFGI + ++ GPE+V+ IP SSVQ AFA++G W DAE++SLHGR +GL + Sbjct: 77 GDPLFFGIGNLIRKKVGPEYVDFIPAPSSVQHAFAKIGIPWDDAEVLSLHGRSSKGLITK 136 Query: 138 IDGKHKIALLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDEL-EKGN 196 + +K+AL TD+ N P I +YL F +E+ AFVCE LGG +E R ++L L E+ Sbjct: 137 LQFLNKVALFTDKINHPKEIASYLLSFNESEWTAFVCENLGGEKERIRKFDLKSLSEEEE 196 Query: 197 FAPLNVVIL-----RWKPGSGTAAVRRGFAYPDEAFRQRRPEKGLITKREVRALVLSELN 251 PLNV++L WKP + V +E + +R P+KGLITK+EVR L ++ LN Sbjct: 197 IDPLNVLVLIRNSVNWKPPTVVPNVS------EENYSKRVPKKGLITKKEVRILSIAFLN 250 Query: 252 LFEEAVVWDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRAD-FEIIRD 310 + E++V+WDIG+GSG++A E A+IA+ GK YAIE E + + N + D +I Sbjct: 251 IREDSVIWDIGAGSGSIAIEAAQIAKNGKSYAIEIDPEGIEICKQNILLQKTDNVHVISG 310 Query: 311 KAPQGLERLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKL 370 KAP+ LE+LPDPD +F+GGS G++ II ++L P G +VI A+TI+ + ++ K Sbjct: 311 KAPEVLEKLPDPDCIFVGGSNGDIYEIIRISLNKLSPLGSLVINAVTIDNVSQIYQSFKK 370 Query: 371 AGMATEVTQLXXXKGKA 387 + EVT L +G+ Sbjct: 371 LKLVPEVTLLNVSRGRV 387 >ref|NP_714704.1| precorrin-6Y methylase [Leptospira interrogans serovar lai str. 56601] gb|AAN51719.1| precorrin-6Y methylase [Leptospira interrogans serovar lai str. 56601] Length = 411 Score = 295 bits (754), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 159/377 (42%), Positives = 234/377 (62%), Gaps = 13/377 (3%) Query: 18 LTPDSLSKVQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLAS 77 L+ + + V ++++L GG+R L FF + GEKIV K+ L ++ E+ E VLAS Sbjct: 17 LSSIAANAVAKAQILAGGKRHLDFFPQFSGEKIVFKDDLTQTIKRIAELSLEHTICVLAS 76 Query: 78 GDPLFFGIAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQR 137 GDPLFFGI + ++ GPE+V+ IP SSVQ AFA++G W DAE++SLHGR +GL + Sbjct: 77 GDPLFFGIGNLIRKKVGPEYVDFIPAPSSVQHAFAKIGIPWDDAEVLSLHGRSSKGLITK 136 Query: 138 IDGKHKIALLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDEL-EKGN 196 + +K+AL TD+ N P I +YL F +E+ AFVCE LGG +E R ++L L E+ Sbjct: 137 LQFLNKVALFTDKINHPKEIASYLLSFNESEWTAFVCENLGGEKERIRKFDLKSLSEEEG 196 Query: 197 FAPLNVVIL-----RWKPGSGTAAVRRGFAYPDEAFRQRRPEKGLITKREVRALVLSELN 251 PLNV++L WKP + V +E + +R P+KGLITK+EVR L ++ LN Sbjct: 197 IDPLNVLVLIRNSVNWKPPTVVPNVS------EENYSKRVPKKGLITKKEVRILSIAFLN 250 Query: 252 LFEEAVVWDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRAD-FEIIRD 310 + E++V+WDIG+GSG++A E A+IA+ GK YAIE E + + N + D +I Sbjct: 251 IREDSVIWDIGAGSGSIAIEAAQIAKNGKSYAIEIDPEGIEICKQNILLQKTDNVHVISG 310 Query: 311 KAPQGLERLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKL 370 KAP+ LE+LPDPD +F+GGS G++ II ++L P G +VI A+TI+ + ++ K Sbjct: 311 KAPEVLEKLPDPDCIFVGGSNGDIYEIIRISLNKLSPLGSLVINAVTIDNVSQIYQSFKK 370 Query: 371 AGMATEVTQLXXXKGKA 387 + EVT L +G+ Sbjct: 371 LKLVPEVTLLNVSRGRV 387 >ref|ZP_00073499.1| COG2242: Precorrin-6B methylase 2 [Trichodesmium erythraeum IMS101] Length = 437 Score = 254 bits (649), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 143/348 (41%), Positives = 204/348 (58%), Gaps = 8/348 (2%) Query: 26 VQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRA-VVLASGDPLFFG 84 V+R+ +L+G +R LS+F E IV+K+ L+A + + + VVLASGDPLFFG Sbjct: 42 VKRATLLVGSDRHLSYFSDNNAESIVIKDLLEAILEIRQHLETPNSCIVVLASGDPLFFG 101 Query: 85 IAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRI-DGKHK 143 + LV F E + PH SSVQLAF+R+ WQD +++S+HGRP+ L + + G K Sbjct: 102 LGRLLVAHFPAEKLRFYPHLSSVQLAFSRIKVPWQDTKIISVHGRPMDELIKALQQGAEK 161 Query: 144 IALLTDETNTPAVIGAYLREFGM-TEYEAFVCERLGGGEEVCRFWELDELEKGNFAPLNV 202 +A+LTD TNTP I L + Y+ +VCE LG +E + W +DE++K FAPLN+ Sbjct: 162 VAVLTDPTNTPQAIANLLLSLHLPATYQFWVCENLGAEDERVQQWSIDEIQKETFAPLNL 221 Query: 203 VILRWKPGSGTAAVRRG----FAYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAVV 258 V+L K V PD F GL++KREVR L+L+EL+L ++ Sbjct: 222 VVLLRKARMANQPVDLVDLPLLGIPDNYFLSYDDHPGLMSKREVRVLILAELSLQPGQII 281 Query: 259 WDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEI-IRDKAPQGLE 317 WDIG+G+G+VA E AR+ VYAIEK+A +E N R F+ + + AP+ L Sbjct: 282 WDIGAGTGSVAVEIARLCSSSIVYAIEKTAIGTSLIEENCRRFQLKNVVSLHGNAPEILH 341 Query: 318 RLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGL 365 L P VFIGGS G+L++I+ C R+ PEG IV+ T+E L+T + Sbjct: 342 HLRPPHRVFIGGSSGKLRNILGVCGIRMLPEGVIVLAFTTLENLHTAI 389 >ref|ZP_00017166.1| hypothetical protein [Chloroflexus aurantiacus] Length = 406 Score = 243 bits (621), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 147/369 (39%), Positives = 208/369 (56%), Gaps = 18/369 (4%) Query: 23 LSKVQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKE-RRAVVLASGDPL 81 + +V R+++L+GG R L+ F + GE++ ++ ++ +L W+ AVVLASGDPL Sbjct: 25 IERVLRADLLVGGRRHLAAFPDFAGERLTIEASVEPALTRLHAAWQNGEVAVVLASGDPL 84 Query: 82 FFGIAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRIDGK 141 ++GI L R F P+ +E++P +S QLAFA L + W DA L+S HGRP+ + + Sbjct: 85 WYGIGSSLRRVFPPDALEIVPAPTSAQLAFAALAEPWHDAVLLSAHGRPLAAIIPAVLTA 144 Query: 142 HKIALLTDETNTPAVIGAYLREFGMT-EYEAFVCERLGGGEEVCRFWELDELEKGNFAPL 200 K A+LTD TPAV+ L G+ E VCE+LGG ++ L ++ + F PL Sbjct: 145 SKAAILTDHHQTPAVVAQALIAAGLAPESRCAVCEQLGGTKQRIVQATLTDIAEATFDPL 204 Query: 201 NVVILRWKPGSGTAAVRRGF--AYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAVV 258 NV+I+ W RR F DEAF E G +TKREVR L L+EL L V+ Sbjct: 205 NVLIV-WNDHP-----RRPFPPGLTDEAFST---EAGQLTKREVRLLSLAELALQPGEVM 255 Query: 259 WDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRA-DFEIIRDKAPQGLE 317 WDIG+GSGAVA E AR +VYA+E+ A + +++ N F A + I +AP Sbjct: 256 WDIGAGSGAVAIEAARACPTAQVYAVERRATFIGHIQTNLVRFPAPNLHITHGEAPAACH 315 Query: 318 RLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEV 377 LPDP A+F+GGSGG L I+A HRLRP GR+V+ I L ++ L A V Sbjct: 316 DLPDPHAIFVGGSGGRLAEIVALARHRLRPGGRLVLNLIMTGHLAEAVQLLPTA----RV 371 Query: 378 TQLXXXKGK 386 TQ+ +G Sbjct: 372 TQIQVNRGT 380 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 573,838,083 Number of extensions: 24478848 Number of successful extensions: 52162 Number of sequences better than 1.0e-05: 184 Number of HSP's gapped: 51831 Number of HSP's successfully gapped: 185 Length of query: 394 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 266 Effective length of database: 409,704,906 Effective search space: 108981504996 Effective search space used: 108981504996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)