BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0046.p (371 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44691 precorrin methylase homolog [imported] - Bacillus me... 358 9e-98 ref|YP_003519.1| precorrin methylase [Leptospira interrogans ser... 263 7e-69 ref|NP_714702.1| cobalamin biosynthesis protein [Leptospira inte... 262 1e-68 gb|EAJ65858.1| unknown [environmental sequence] 226 5e-58 ref|NP_954035.1| cobalamin biosynthesis protein CbiG, putative [... 215 1e-54 >pir||T44691 precorrin methylase homolog [imported] - Bacillus megaterium emb|CAA04315.1| CbiG protein [Bacillus megaterium] Length = 374 Score = 358 bits (920), Expect = 9e-98, Method: Compositional matrix adjust. Identities = 180/359 (50%), Positives = 251/359 (69%), Gaps = 4/359 (1%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKY-SGNVQDATGHGQVFAEPVKELLPKLFR 63 YA VAIT +G+ +A LG ++V+ +K+ G+ Q+ + Q+F+ V+ LLP LF Sbjct: 18 YAVVAITKHGVEIARNLGRIFQQSDVYYMSKFEKGDEQEQ--NIQMFSGSVRMLLPSLFE 75 Query: 64 QYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVA 123 Y+GLI+ SLGA VR+IAP+L DK+TDPAV+VID++GE+VIS+LSGH+GGAN LTR+VA Sbjct: 76 SYKGLIIIISLGAVVRMIAPILKDKKTDPAVVVIDDKGENVISVLSGHIGGANELTREVA 135 Query: 124 QLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGER 183 + +HPVITTASDVQ VDL G FGW E + VSA++VN E +A +QESGE+ Sbjct: 136 AALRAHPVITTASDVQKTIPVDLFGKRFGWVWESAENVTPVSASVVNEEEIAVVQESGEK 195 Query: 184 GWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSLVL 243 W +P +++ S+ EA+ AA+VVT R L ++E A L E V+YRP+ L + Sbjct: 196 SWWHYEHPVPANIKTYSSIQTALEASPHAALVVTHRDLKKEEEAIL-ENGVLYRPKVLAI 254 Query: 244 GLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELALF 303 G+GCNRGT+ E+E V+ TLA++ S+ SV+ + T +K DE GLL + +KYGWE + Sbjct: 255 GMGCNRGTSAAEIETVIEKTLAELQFSMKSVKALCTIELKKDEEGLLEVASKYGWEFVYY 314 Query: 304 SPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARVSF 362 SP++LN++ ++QPS+ VFK TGAYGV EPAA+L SGA +L L KKKSGN TI+VA + + Sbjct: 315 SPQELNSISIQQPSDTVFKYTGAYGVSEPAAMLYSGADTLELVKKKSGNVTISVALIPY 373 >ref|YP_003519.1| precorrin methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] gb|AAS72156.1| precorrin methylase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 385 Score = 263 bits (671), Expect = 7e-69, Method: Compositional matrix adjust. Identities = 144/366 (39%), Positives = 218/366 (59%), Gaps = 11/366 (3%) Query: 3 KRYAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHGQVFAEPVKELLPKLF 62 K+Y+ IT +G+ +A K+ D ++F AK+ +QDA + ++ + P+ + L + F Sbjct: 6 KQYSVFVITKHGLEIAKKIQAIWQDIDLFVSAKF---IQDAPSNSKLLSFPMDKTLRETF 62 Query: 63 RQYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQV 122 R+Y+ I S+GA VR+IAPLL +K+ DPAV+ +D++ + I +LSGH+G N T ++ Sbjct: 63 REYDCHIFIISVGAVVRMIAPLLENKKVDPAVLCVDDKAKFSICVLSGHVGRGNFFTEKL 122 Query: 123 AQLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTV-SAALVNGEAVAFLQESG 181 ++++++ PVITTASDV G VD+LG EFGW E T AA+VN V F+QE+G Sbjct: 123 SEILNNIPVITTASDVSGTLTVDILGREFGWILENPDRNITKGCAAVVNETKVLFIQETG 182 Query: 182 ERGWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSL 241 E W P LP+ V S+ +Q + ++ TDR + + +V+Y ++L Sbjct: 183 EPNWWPLDKPLPKGVEYSISLNNVQPEDYEILLIATDRSNVHKTHKEHYKNSVLYYTKTL 242 Query: 242 VLGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELA 301 +LGLGC+R E +E + L + G ++ SVR +AT +K +E+ L L KYGWEL Sbjct: 243 ILGLGCDRNIPFEIVENGIYKILDENGFAIQSVRAIATIDLKKEESAFLKLSQKYGWELI 302 Query: 302 LFSPEQLNTVV-LEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKK------SGNAT 354 FSPE+L+ V + PSEIV TG V E A+LL+SGA SL+L K+K N T Sbjct: 303 FFSPEELDRVSQVRSPSEIVKGFTGTKSVSEAASLLASGADSLLLTKQKYKEEPNGKNLT 362 Query: 355 IAVARV 360 IAV+R+ Sbjct: 363 IAVSRI 368 >ref|NP_714702.1| cobalamin biosynthesis protein [Leptospira interrogans serovar lai str. 56601] gb|AAN51717.1| cobalamin biosynthesis protein [Leptospira interrogans serovar lai str. 56601] Length = 385 Score = 262 bits (669), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 144/366 (39%), Positives = 218/366 (59%), Gaps = 11/366 (3%) Query: 3 KRYAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHGQVFAEPVKELLPKLF 62 K+Y+ IT +G+ +A K+ D ++F AK+ +QDA + ++ + P+ + L + F Sbjct: 6 KQYSVFVITKHGLEIAKKIQAIWQDIDLFVSAKF---IQDAPSNSKLLSFPMDKTLRETF 62 Query: 63 RQYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQV 122 R+Y+ I S+GA VR+IAPLL +K+ DPAV+ +D++ + I +LSGH+G N T ++ Sbjct: 63 REYDCHIFIISVGAVVRMIAPLLENKKVDPAVLCVDDKAKFSICVLSGHVGRGNFFTEKL 122 Query: 123 AQLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTV-SAALVNGEAVAFLQESG 181 ++++++ PVITTASDV G VD+LG EFGW E T AA+VN V F+QE+G Sbjct: 123 SEILNNIPVITTASDVSGTLTVDILGREFGWILENPDRNITKGCAAVVNETKVLFIQETG 182 Query: 182 ERGWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSL 241 E W P LP+ V S+ +Q + ++ TDR + + +V+Y ++L Sbjct: 183 EPNWWPLDRPLPKGVEYSISLNNVQPEDYEILLIATDRSNVHKTHKEHYKNSVLYYTKTL 242 Query: 242 VLGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELA 301 +LGLGC+R E +E + L + G ++ SVR +AT +K +E+ L L KYGWEL Sbjct: 243 ILGLGCDRNIPFEIVENGIYKILDENGFAIQSVRAIATIDLKKEESAFLKLSQKYGWELI 302 Query: 302 LFSPEQLNTVV-LEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKK------SGNAT 354 FSPE+L+ V + PSEIV TG V E A+LL+SGA SL+L K+K N T Sbjct: 303 FFSPEELDRVSQVRSPSEIVKGFTGTKSVSEAASLLASGADSLLLTKQKYKEEPNGKNLT 362 Query: 355 IAVARV 360 IAV+R+ Sbjct: 363 IAVSRI 368 >gb|EAJ65858.1| unknown [environmental sequence] Length = 352 Score = 226 bits (577), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 126/358 (35%), Positives = 201/358 (56%), Gaps = 9/358 (2%) Query: 3 KRYAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHGQVFAEPVKELLPKLF 62 K+ A +AIT NGI + LKL D E+F K+S N + Q + E + + LF Sbjct: 2 KKIAVLAITKNGIEIGLKLKGYFPDFEIFAPIKFSDNNEKI----QWYDESTSQKIVNLF 57 Query: 63 RQYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQV 122 ++ +G+I FSLGA +RL+AP + DK+TDPAVIVID+ VIS+LSGHLGGAN L+ ++ Sbjct: 58 KENDGIICLFSLGAVIRLLAPHIKDKKTDPAVIVIDDNANFVISVLSGHLGGANELSNEI 117 Query: 123 AQLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGE 182 A+ M + VITTA+DV AVDL+G EFGW+ + + +SA +VN E + Q +G+ Sbjct: 118 AEKMGATSVITTAADVNKTIAVDLVGKEFGWKIHDDSNVTRISAFMVNKEQIGVFQNAGQ 177 Query: 183 RGWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSLV 242 + W +PE++ ++ +L+ + +++T+ ++ ++++ + +VVYR LV Sbjct: 178 KEWWK--GKMPENITFFSNIEDLKNSDSKGYLIITNDEIKDEDVL---KNSVVYRVPDLV 232 Query: 243 LGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELAL 302 +G+G + T +E + V +TL GL + + D GL+ L + + Sbjct: 233 IGIGLHWDTPKETILNGVNETLEKFGLKQNKIARFVSIKKDKDVIGLIELGKEMDIPVEY 292 Query: 303 FSPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARV 360 E+L T+ PS+ V G V E AA+ SS +V K+K N T+A+AR+ Sbjct: 293 IDREELATISAPNPSKTVQAFEGTASVSEAAAIKSSQGELIVEKQKFPPNLTVAIARI 350 >ref|NP_954035.1| cobalamin biosynthesis protein CbiG, putative [Geobacter sulfurreducens PCA] gb|AAR36385.1| cobalamin biosynthesis protein CbiG, putative [Geobacter sulfurreducens PCA] Length = 351 Score = 215 bits (548), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 139/362 (38%), Positives = 202/362 (55%), Gaps = 18/362 (4%) Query: 4 RYAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHGQV---FAEPVKELLPK 60 R A +AIT NG RL +L E L ++ K++G G+V FA +K L+ + Sbjct: 2 RIAIIAITRNGARLGSQLREGLGTADLHVLQKFAGQA------GKVAVPFAGDLKGLVAR 55 Query: 61 LFRQYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTR 120 L+ +G + + G VR+IAPLL K DPAV+V+DE G +S+LSGHLGGAN L Sbjct: 56 LWPDVKGFVFIMAAGIVVRMIAPLLESKDKDPAVVVMDEGGRFAVSLLSGHLGGANELAC 115 Query: 121 QVAQLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQES 180 + A + + VITTA+D + + DLL E GW E + +KT++A L+ GE +A + + Sbjct: 116 RCAHVTGAREVITTATDANDLPSFDLLAKEAGWAIEDLSRIKTLNALLLEGEEIAVVDPT 175 Query: 181 GE-RGWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPR 239 R + A L H + + ++ V T+R+LP Q + + ++ RPR Sbjct: 176 DRVRAYFNGKARLTFH----DTFVQALQSGAKGFVFATNRRLPPQ---SQSDALLILRPR 228 Query: 240 SLVLGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWE 299 +L LG+GCN GT EE+E VV L + L+L SV + +A K E GLL ++ E Sbjct: 229 NLALGIGCNSGTPAEEIEEVVTVQLKRLFLALKSVAVIGSAMAKRGEEGLLRFAERHCIE 288 Query: 300 LALFSPEQLNTV-VLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVA 358 L + E+LN V V PS +A GA GV EP+A+L+S SL+LKK KSGN T+A+A Sbjct: 289 LRFYESEELNGVAVPSPPSAHALEAIGAVGVAEPSAVLASNGGSLLLKKVKSGNVTLAIA 348 Query: 359 RV 360 + Sbjct: 349 EI 350 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 494,336,447 Number of extensions: 19021859 Number of successful extensions: 46979 Number of sequences better than 1.0e-05: 131 Number of HSP's gapped: 46839 Number of HSP's successfully gapped: 142 Length of query: 371 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 244 Effective length of database: 412,420,005 Effective search space: 100630481220 Effective search space used: 100630481220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)