BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0045.p (574 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|O87698|COBB_BACME Cobyrinic acid A,C-diamide synthase >gi|112... 234 3e-60 ref|NP_242764.1| cobyrinic acid-diamide synthase [Bacillus halod... 221 4e-56 ref|ZP_00180576.1| hypothetical protein Mther148401 [Moorella th... 192 2e-47 ref|NP_470491.1| similar to cobyrinic acid a,c-diamide synthase ... 181 6e-44 ref|NP_071054.1| cobyrinic acid a,c-diamide synthase (cbiA) [Arc... 179 1e-43 >sp|O87698|COBB_BACME Cobyrinic acid A,C-diamide synthase pir||T44692 cobyrinic acid a,c-diamide synthase [imported] - Bacillus megaterium emb|CAA04316.1| CbiA protein [Bacillus megaterium] Length = 460 Score = 234 bits (598), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 111/198 (56%), Positives = 139/198 (70%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ FKCGPDYIDP YH AVT RT+RN+DSWM + E R+S+ AD+S++EGVMG + Sbjct: 35 VQGFKCGPDYIDPTYHTAVTERTSRNIDSWMLEHDMVREIVARASQDADISIMEGVMGFF 94 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 DGK GSTAEI+L+T SPVLLVV+ SM RSAAAIV GFQQ P+ I VI NR Sbjct: 95 DGKNPLTNEGSTAEISLITNSPVLLVVNCASMARSAAAIVKGFQQFLPEANIVGVIANRV 154 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADLL 225 GS HY+ VKAAIE C IPV+G+L + L IPERHLGL+P++ERGEL P F++ L+ Sbjct: 155 GSEGHYKLVKAAIEQECHIPVVGYLKTNDELTIPERHLGLIPSIERGELTPFFEQLGQLV 214 Query: 226 EAGTDLEVLLQIAAAASV 243 D+E + ++A A + Sbjct: 215 HDTIDIEKVYELALAPKI 232 Score = 167 bits (423), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 86/220 (39%), Positives = 112/220 (50%), Gaps = 10/220 (4%) Query: 355 PAPVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFA 414 P IAVA+DAAFNFYY +N FSPL GE +P +ADG+Y+GGGFPEEFA Sbjct: 245 PQVKIAVARDAAFNFYYEENFELLKACGAELVEFSPLKGEMVPQDADGLYIGGGFPEEFA 304 Query: 415 ATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDR 474 T+A ECGG+M L + +EM G+IP MQ + Sbjct: 305 ETLAQQIDVKNSVRAAIQKGLPTLAECGGFMFLTDGIVTTDDTCYEMVGLIPGQVRMQTK 364 Query: 475 RTALGYREVTARADCLLLKQGERLRGHEFH----YSQMSYSPGEPQLYAYDSRGRSGSQP 530 ALGYREVT + L K + +GHEFH YS+ +SP AYD++G G + Sbjct: 365 LAALGYREVTGKPGNFLFKGDIQAKGHEFHYSTFYSEKEFSP------AYDTKGMRGMKE 418 Query: 531 EGYARGNIMAAYAHIHLASHIPAAIRLVEACRSYHRSNKD 570 EGY N++A Y H H S VE C++ + +D Sbjct: 419 EGYMNNNLIAGYTHFHFGSSTKMVENWVEQCKAAKKERRD 458 >ref|NP_242764.1| cobyrinic acid-diamide synthase [Bacillus halodurans] sp|Q9KBM8|COBB_BACHD Cobyrinic acid A,C-diamide synthase pir||B83887 cobyrinic acid-diamide synthase BH1898 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05617.1| cobyrinic acid-diamide synthase [Bacillus halodurans] Length = 465 Score = 221 bits (563), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 111/199 (55%), Positives = 138/199 (69%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ FKCGPDYIDP+YH AVT R +RNLDSWM S + + F+ GADLS+IEGVMG Y Sbjct: 34 VQGFKCGPDYIDPSYHRAVTKRASRNLDSWMVSEDVVNDIFIEGCNGADLSIIEGVMGFY 93 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 DGK +T GSTA+I+LL +PVLLVVD+ M RSAAA+V GFQQL V+IA VI+N Sbjct: 94 DGKASTSDKGSTADISLLLQAPVLLVVDISGMARSAAAVVKGFQQLNEKVKIAGVILNGA 153 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADLL 225 GS H AI+ ACGIP +G+L + IPERHLGL+PA+ERGE + LF + A ++ Sbjct: 154 GSERHAEMCSEAIKEACGIPALGYLKKGDVPSIPERHLGLVPALERGEHDSLFSQLAAVV 213 Query: 226 EAGTDLEVLLQIAAAASVL 244 DLE + ++A AS L Sbjct: 214 SRQFDLERIERLALEASAL 232 Score = 164 bits (415), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 87/206 (42%), Positives = 113/206 (54%), Gaps = 2/206 (0%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 IAVA DAAF+FYY +N FSPL GE +P DG+YLGGGFPEEFA ++ Sbjct: 250 IAVAYDAAFHFYYEENFTLLRQAGAELVYFSPLRGEDLPSHCDGLYLGGGFPEEFADELS 309 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDRRTAL 478 ECGGYM L LT G EMAG+IP M+++ +L Sbjct: 310 QWIKDHPQLKKRVQSGLPTLAECGGYMFLGEQLTTTKGKTVEMAGVIPMVVQMENKLVSL 369 Query: 479 GYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQPEGYARGNI 538 GYR+VT D LL +G+++RGHEFHYS+ Y+ E + AY +G G EGY ++ Sbjct: 370 GYRDVTPLDDSFLLSKGQQIRGHEFHYSKAVYT--ETERPAYFVKGLFGEGKEGYMSDHL 427 Query: 539 MAAYAHIHLASHIPAAIRLVEACRSY 564 +A Y H+H AS A + V+AC Y Sbjct: 428 LAGYTHLHFASEPAVATQWVKACEVY 453 >ref|ZP_00180576.1| hypothetical protein Mther148401 [Moorella thermoacetica ATCC 39073] Length = 466 Score = 192 bits (487), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 105/208 (50%), Positives = 136/208 (65%), Gaps = 3/208 (1%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ FK GPDYIDP YH TGR +RNLD+++ + +LE F R++ +D++VIEGVMGLY Sbjct: 35 VQGFKVGPDYIDPGYHTLATGRPSRNLDTYLMTPAAVLEAFERAAATSDIAVIEGVMGLY 94 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 DG TG +GSTA IA L +PVLLVVD S+G+S AA VLG++ L+P V +A VI+NR Sbjct: 95 DGHRDTG-SGSTATIARLLAAPVLLVVDATSLGQSVAAEVLGYRSLDPGVNLAGVILNRV 153 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADLL 225 S H ++ AIE GIPV+GWL R +P RHLGL+PA E+ +L P+ A + Sbjct: 154 SSEGHLEVLRQAIEEYTGIPVVGWLRRGSLPPLPSRHLGLIPAGEQEDLRPVLAELAATI 213 Query: 226 EAGTDLEVLLQIAAAASVLRYPAAGKIL 253 G DLE +L +A A L PA G L Sbjct: 214 ATGLDLERVLDLATQAGPL--PAGGSRL 239 Score = 122 bits (306), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 83/216 (38%), Positives = 107/216 (49%), Gaps = 4/216 (1%) Query: 350 ASVYDPAPVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGF 409 A V + PV AVA D AFNFYY D+ PFSPL + +P A GI +GGGF Sbjct: 245 AGVREKIPV-AVALDKAFNFYYQDSLDYLAVLGAELLPFSPLEDDRLPAGAAGIIIGGGF 303 Query: 410 PEEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHS 469 PE F A + N ECGG M L R + D G+ MAGI+PA Sbjct: 304 PEIFLAPLTDNRPLLADLRRQVARGIPLYAECGGLMYLAREIIDLEGSKWPMAGIVPAAC 363 Query: 470 VMQDRRTALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGE-PQLYAYDSRGRSGS 528 MQ LGYRE + L+ + + LRGHEFHYS M+ + P Y + RG Sbjct: 364 RMQKSLAGLGYREARLCRETLVGHRDDCLRGHEFHYSTMTSKDTDFPPAYTWKHRG--SI 421 Query: 529 QPEGYARGNIMAAYAHIHLASHIPAAIRLVEACRSY 564 +GY I+A+Y H+H ++ AA + ACR+Y Sbjct: 422 WYDGYGTRQIVASYLHLHFLGNVGAAQNFLAACRAY 457 >ref|NP_470491.1| similar to cobyrinic acid a,c-diamide synthase [Listeria innocua] sp|Q92CL7|COBB_LISIN Cobyrinic acid A,C-diamide synthase pir||AI1576 cobyrinic acid a,c-diamide synthase homolog cbiA [imported] - Listeria innocua (strain Clip11262) emb|CAC96385.1| cbiA [Listeria innocua] Length = 452 Score = 181 bits (458), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 93/205 (45%), Positives = 132/205 (64%), Gaps = 1/205 (0%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYML-EYFLRSSEGADLSVIEGVMGL 104 VQ FK GPDYID YH A+TG + NLDS++ ML F + E AD+SVIEGVMGL Sbjct: 33 VQPFKVGPDYIDTNYHQAITGVASINLDSFLIDDDEMLASLFQKHGESADISVIEGVMGL 92 Query: 105 YDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNR 164 +DG ST+ IA T +PV+LVVD +++ SAAAIV GF + +P+++IA VI+NR Sbjct: 93 FDGLGTDRDNASTSFIAKCTKTPVILVVDGKAISTSAAAIVDGFNRFDPELKIAGVIINR 152 Query: 165 CGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADL 224 S +H+ +K AIE +PV+G+L ++ + +PERHLGL+P E ELE ++ +DL Sbjct: 153 VASENHFSLIKGAIERYTDVPVLGYLPKNAAVALPERHLGLVPQEEMTELEAKWELLSDL 212 Query: 225 LEAGTDLEVLLQIAAAASVLRYPAA 249 + DL+ LL+I+ ++ L A Sbjct: 213 ITTHVDLDKLLEISKSSENLSTSKA 237 Score = 108 bits (271), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 71/193 (36%), Positives = 89/193 (46%), Gaps = 9/193 (4%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 +A A DAAF+FYY DN PFSPL +P EAD IY+GGGFPE FA + Sbjct: 249 VAYALDAAFHFYYQDNLDLIRLTGAELIPFSPLEDNEVP-EADFIYIGGGFPEIFAKQLN 307 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDRRTAL 478 N ECGG M L SL + G +EM G+ S M R Sbjct: 308 DNRSMRKSILAAHEKGIPIYAECGGLMYLGSSL-EMEGKQYEMVGVFNGISKMTTRLRKF 366 Query: 479 GYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQPE----GYA 534 GY + L+ K+G +RGHEFH+S + E S+ R G + GY Sbjct: 367 GYCIAEPLEETLIGKKGMSIRGHEFHHS--VFETTETACMKL-SKKRDGEIVKEWRGGYQ 423 Query: 535 RGNIMAAYAHIHL 547 +GN A+Y HIH Sbjct: 424 KGNTFASYLHIHF 436 >ref|NP_071054.1| cobyrinic acid a,c-diamide synthase (cbiA) [Archaeoglobus fulgidus DSM 4304] sp|O28054|COBB_ARCFU Probable cobyrinic acid A,C-diamide synthase pir||E69528 cobyrinic acid a,c-diamide synthase (cbiA) homolog - Archaeoglobus fulgidus gb|AAB89025.1| cobyrinic acid a,c-diamide synthase (cbiA) [Archaeoglobus fulgidus DSM 4304] Length = 458 Score = 179 bits (455), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 86/196 (43%), Positives = 131/196 (66%), Gaps = 1/196 (0%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ +K GPD+IDP +H TGR +RNLDS+M S +LE F+R+ GAD+++IEG GLY Sbjct: 40 VQPYKVGPDFIDPGFHHLATGRYSRNLDSFMLSRSAILETFIRNFRGADVAIIEGKTGLY 99 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 D +A GS AE++ + +PV+LV +V + R+AAAI+LG++ +PDV + VI+NR Sbjct: 100 DSSDAVSEKGSVAEVSKILKAPVVLVANVERLNRTAAAIILGYKLFDPDVLLKGVILNRV 159 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADLL 225 GS H V+ A+E G+ V+G + R + + +P RHLGL+ A ER +++ L D A+++ Sbjct: 160 GSERHAGKVRTAVEKLAGVRVLGVVPRKK-VKMPYRHLGLVTAYEREDMDELLDNIAEIV 218 Query: 226 EAGTDLEVLLQIAAAA 241 E D++ +L+IA A Sbjct: 219 EKHVDVDKILEIAEKA 234 Score = 95.1 bits (235), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 65/222 (29%), Positives = 94/222 (42%), Gaps = 5/222 (2%) Query: 339 PPLDRSAPQALASVYDPAPVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPP 398 PPLD I V +D F+FYY DN + L+ + +P Sbjct: 235 PPLDSVFEDEKEDEEKKYVKIGVIRDQVFSFYYQDNLDELSKYAELVF-VNSLTDKRLP- 292 Query: 399 EADGIYLGGGFPEEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAV 458 + D +Y+GGGFPE FA + N ECGG M L SL G Sbjct: 293 DVDALYIGGGFPEVFAEGLEKNEKLRNEIYDFCQSGNPVYAECGGLMYLGESLETSEGE- 351 Query: 459 HEMAGIIPAHSVMQDRRTALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLY 518 EM G +P + M +R A GY C++ K+ +++ GHEFHYS+ + + G+ Sbjct: 352 FEMVGFLPLKTKMYERFQAQGYSVYRTLKPCIIAKRNQKIVGHEFHYSRPTLT-GKADFA 410 Query: 519 AYDSRGRS-GSQPEGYARGNIMAAYAHIHLASHIPAAIRLVE 559 RG + +G + N + Y H+H S A R V+ Sbjct: 411 FRVERGFGIDGRRDGILKENTLGCYIHVHFLSDKSIARRFVK 452 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 741,981,733 Number of extensions: 28347897 Number of successful extensions: 66908 Number of sequences better than 1.0e-05: 194 Number of HSP's gapped: 66267 Number of HSP's successfully gapped: 317 Length of query: 574 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 443 Effective length of database: 401,559,609 Effective search space: 177890906787 Effective search space used: 177890906787 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)