BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0022.p (479 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00180513.1| hypothetical protein Mther151501 [Moorella th... 236 1e-60 ref|ZP_00019478.1| hypothetical protein [Chloroflexus aurantiacus] 199 2e-49 ref|NP_622668.1| Sensory transduction histidine kinases [Thermoa... 178 3e-43 ref|ZP_00097541.1| COG0642: Signal transduction histidine kinase... 172 2e-41 ref|NP_951511.1| sensor histidine kinase [Geobacter sulfurreduce... 165 2e-39 >ref|ZP_00180513.1| hypothetical protein Mther151501 [Moorella thermoacetica ATCC 39073] Length = 241 Score = 236 bits (601), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 123/253 (48%), Positives = 177/253 (69%), Gaps = 17/253 (6%) Query: 224 MLGRMEGFYTELEDSYATQRRFVSDASHELRTPLTTIRGNIDLLQKIWEMNPDESRMTEA 283 ML R++ YT LE++Y+ QRRFV+DASHELRTPLTTIRGN+DLL+K+ + Sbjct: 1 MLARLQRAYTRLEEAYSAQRRFVADASHELRTPLTTIRGNVDLLRKV-----------QG 49 Query: 284 EIRQLSIESVKDIADESKRMSRLVSDMLSLARADTGRTFDIEPVALEPMMTEVARRASFL 343 + E++ DIA E++RMSRLV+D+L+LARAD G+ EP+ + P++ EVAR+A L Sbjct: 50 QGEAWQDEALADIASEAERMSRLVNDLLTLARADAGQEIKREPLEILPLLQEVARQAPLL 109 Query: 344 PRQAEWSVGNLGHLNGKYIVGNKDYLQQMLFIFIDNAFKYTPSGEVAMDAVFY---QNQV 400 ++G L +L G +I+GN+DYL+Q+ FI +DNAFKYTPS E +D + Q ++ Sbjct: 110 GTATFTAIG-LENLAGVHIMGNRDYLKQLFFILLDNAFKYTPS-EGKIDLIVNVEPQQRL 167 Query: 401 GIRISDTGIGMDKDEVPHIFDRFYRADESRGITEGIGLGLSIAKWIIDEHGGSVEVVTRQ 460 I++ DTG G+ ++ HIF+RFYRAD +R +EG GLGL+IA+WI+++H G + V + Sbjct: 168 IIKVRDTGPGIPPRDLEHIFERFYRADATRS-SEGTGLGLAIARWIVEQHQGHIGVESTV 226 Query: 461 GEGTTFVIWLPLL 473 G+GTTF I +PLL Sbjct: 227 GKGTTFTITIPLL 239 >ref|ZP_00019478.1| hypothetical protein [Chloroflexus aurantiacus] Length = 539 Score = 199 bits (505), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 149/497 (29%), Positives = 245/497 (49%), Gaps = 46/497 (9%) Query: 1 LRLTAWYSGILAVMLLALSAAIYGFVYFNTYGDIKDRLKNQASQIV-------LQPVTYL 53 +RLT Y+G+ A+ L + ++ V Y ++ L + + Q+ Q + Sbjct: 59 VRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVGFQSIRTA 118 Query: 54 DGSSNIFVGGNLEGQNLFAQ----MYIYDIDKIIPNN--NMKTFNLRFNVPEQETIVS-- 105 G +++ N + LF + + ID + ++ N+++ L PE +S Sbjct: 119 SGELQLYL--NRDFIKLFTNRGVGVQFFRIDGTLASSTPNLESHMLPLP-PEAMQYISAG 175 Query: 106 --QQGFVRASYDGNPFLIYQRPVDIPGYDGNPAAVLQMAAYTGEQDRLMLRLKNILIVGS 163 Q VR +G P PV + +G LQ++ + + L+ ILI G Sbjct: 176 NDQMSVVR-EINGVPVKSLITPVTL--RNGQLIGFLQVSRPLDDVVNTLTLLQQILIGGG 232 Query: 164 FATLIAAFTFGLFLARKAMSPIGKVIEAANGIQTGTDLSSRIVYDGPQDEIGRLIQTVNS 223 LI ++L+++A+ PI ++ A I DL RI +DE+ L T+N Sbjct: 233 LLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTINE 292 Query: 224 MLGRMEGFYTELEDSYATQRRFVSDASHELRTPLTTIRGNIDLLQKIWEMNPDESRMTEA 283 +L R+E + TQ+RFV+D SHELRTPLT ++GN+++L + PD + + E Sbjct: 293 LLSRLERLFV-------TQQRFVADVSHELRTPLTAMQGNLEILDR--GTCPDPALLKEV 343 Query: 284 EIRQLSIESVKDIADESKRMSRLVSDMLSLARADTGRTFDIEPVALEPMMTEVARRASFL 343 + D+ E+ R+ R+V+D+L LA+++T EPV L+ ++ EV R L Sbjct: 344 ---------IGDMRRETARLIRMVNDLLLLAQSETHVVLRHEPVELDTLLLEVFRELRPL 394 Query: 344 PRQAEWSVGNLGHLNGKYIVGNKDYLQQMLFIFIDNAFKYTPSGE-VAMDAVFYQNQVGI 402 Q +G + + G++D ++Q L NA +YTP G V + Y+ + Sbjct: 395 AGQVRLRIGAEDQI---LVYGDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACL 451 Query: 403 RISDTGIGMDKDEVPHIFDRFYRADESRG-ITEGIGLGLSIAKWIIDEHGGSVEVVTRQG 461 ++DTG+G+ ++ HIF+RFYR D SR + G GLGLSI KW+ + HGG V V + G Sbjct: 452 SVADTGVGISEEARAHIFERFYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVG 511 Query: 462 EGTTFVIWLPLLFAPPL 478 G+TF I+LPL A P+ Sbjct: 512 RGSTFAIYLPLPEANPV 528 >ref|NP_622668.1| Sensory transduction histidine kinases [Thermoanaerobacter tengcongensis] gb|AAM24272.1| Sensory transduction histidine kinases [Thermoanaerobacter tengcongensis] Length = 462 Score = 178 bits (451), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 114/314 (36%), Positives = 180/314 (57%), Gaps = 31/314 (9%) Query: 165 ATLIAAFTFGLFLARKAMSPIGKVIEAANGIQTGTDLSSRIVYDGPQDEIGRLIQTVNSM 224 A + +F G F+ ++A+ P+ +I+ I L+ R+ G +DE+ RL +T N M Sbjct: 171 AGIFISFVTGYFVTKRALRPVDYMIKEVKDID-AKGLNKRLKVYGEEDELTRLAKTFNDM 229 Query: 225 LGRMEGFYTELEDSYATQRRFVSDASHELRTPLTTIRGNIDLLQKIWEMNPDESRMTEAE 284 L R LE+S+A Q RFVSDASHELRTP++ I+G ID+L + W D+ + E Sbjct: 230 LDR-------LEESFARQNRFVSDASHELRTPISVIKGYIDMLDR-W--GKDDREVLE-- 277 Query: 285 IRQLSIESVKDIADESKRMSRLVSDMLSLARADTGRTFDIEPVA--LEPMMTEVARRASF 342 E +K I E+ M LV +L LA+ D R+ +E + L+ + EV R Sbjct: 278 ------EGIKAIKKETLEMESLVEKLLFLAKGDD-RSIKLERESFDLKEIAEEVVREIKL 330 Query: 343 LPRQAEWSV-GNLGHLNGKYIVGNKDYLQQMLFIFIDNAFKYT-PSGEVAMDAVFYQNQV 400 + S+ G +N +K ++++L I +DNA KYT +G + ++ + ++ Sbjct: 331 IYEGKNVSLKGENVRINA-----DKKLIKEVLRILLDNAAKYTSKNGNIEIE-IGSGDEA 384 Query: 401 GIRISDTGIGMDKDEVPHIFDRFYRADESRGI-TEGIGLGLSIAKWIIDEHGGSVEVVTR 459 I++ D GIG+ ++++P+IF+RFYR D++R T G GLGLSIAKWI++EHGG + V + Sbjct: 385 YIKVKDDGIGIPEEDLPYIFERFYRVDKARSKDTGGTGLGLSIAKWIVEEHGGVIGVKSE 444 Query: 460 QGEGTTFVIWLPLL 473 G+GT F + LPL+ Sbjct: 445 VGKGTEFTVMLPLI 458 >ref|ZP_00097541.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 494 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 118/347 (34%), Positives = 173/347 (49%), Gaps = 45/347 (12%) Query: 150 RLMLRLKNILIVGSFATLIAAFTFGLFLARKAMSPIGKVIEAANGIQ------------- 196 R++ + I+++ TL+ + G + R+ + PI ++ E A + Sbjct: 162 RILAAMFGIMLLIEGITLLRSIASGARVIRRTLQPIEQLAETARNLSHEGIFSPEQLKDL 221 Query: 197 -------TGTDLSSRIVYDGPQDEIGRLIQTVNSMLGRMEGFYTELEDSYATQRRFVSDA 249 T L +RI D Q E+ L +N ML R + +SY +Q RFVSDA Sbjct: 222 AGKLDDINATHLDTRISVDETQSELQSLAAAINGMLDR-------INESYRSQARFVSDA 274 Query: 250 SHELRTPLTTIRGNIDLLQKIWEMNPDESRMTEAEIRQLSIESVKDIADESKRMSRLVSD 309 SHELRTP++ I+G +LL + W DE + ES+ I +E+ M LV Sbjct: 275 SHELRTPISVIQGYANLLDR-WGKK-DEKTLQ---------ESIDAIKNETAHMKTLVEQ 323 Query: 310 MLSLARADTGR-TFDIEPVALEPMMTEVARRASFLPRQAEWSVGNLGHLNGKYIVGNKDY 368 +L LAR D IE L + E+ R + E+S L YI G+K Sbjct: 324 LLFLARGDNNTMALQIESFELSALAQEILRETQMIDGGHEYST----ILTPVYIKGDKGL 379 Query: 369 LQQMLFIFIDNAFKYTPSG-EVAMDAVFYQNQVGIRISDTGIGMDKDEVPHIFDRFYRAD 427 ++Q I +DNA KYTP+G ++ + N + + D GIG++ + VP IFDRF+R D Sbjct: 380 IKQATRILMDNAMKYTPAGKQITLSVSSKDNLARLTVQDEGIGIEPEAVPLIFDRFFRTD 439 Query: 428 ESRG-ITEGIGLGLSIAKWIIDEHGGSVEVVTRQGEGTTFVIWLPLL 473 ESR T G GLGL+IAKWI + HGG +EV++R GT I LP++ Sbjct: 440 ESRTRATGGSGLGLAIAKWITERHGGHLEVLSRLEVGTRISIVLPMV 486 >ref|NP_951511.1| sensor histidine kinase [Geobacter sulfurreducens PCA] gb|AAR33784.1| sensor histidine kinase [Geobacter sulfurreducens PCA] Length = 469 Score = 165 bits (418), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 139/488 (28%), Positives = 231/488 (47%), Gaps = 44/488 (9%) Query: 3 LTAWYSGILAVMLLALSAAIYGFVYFNTYGDIKDRLKNQASQIVLQPV---------TYL 53 LT WY+ LAV+L+ S+ IY V N DR ++ V P + Sbjct: 10 LTLWYAVTLAVILVLFSSFIY-LVLSNQLNKGIDRELLTVAEAVASPTLEPFRHAAPSVF 68 Query: 54 DGSSNIFVGGNLEGQNLFAQMYIYDIDKIIPNNNMKTFNLRFNVPEQETIVSQQGFV--- 110 D F+G L G++ + + D + ++ LR + + Q G V Sbjct: 69 DQVLEDFIGTRLTGKH----VQVLDGSGAVAASSKSMEELRIPLGKTALRRVQAGKVSYE 124 Query: 111 -RASYDGNPFLIYQRPVDIPGYDGNPAAVLQMAAYTGEQDRLMLRLKNILIVGSFATLIA 169 R + D P P+ DG ++ + + +++ + V LI Sbjct: 125 TRVNLDVYPVRTIVYPI---MTDGRLDQIVLVGTSMRGPAETLEKVQLVFAVSIPLALIL 181 Query: 170 AFTFGLFLARKAMSPIGKVIEAANGIQTGTDLSSRIVYDGPQDEIGRLIQTVNSMLGRME 229 G FLA +A+ P+ + +A I T +L R+ PQDEIGRL T N L R Sbjct: 182 WSLGGWFLAGRALKPVDLITRSARKI-TAENLGLRLEVINPQDEIGRLATTFNDTLER-- 238 Query: 230 GFYTELEDSYATQRRFVSDASHELRTPLTTIRGNIDLLQKIWEMNPDESRMTEAEIRQLS 289 LE+++ R+F D SHELRTPLT +RG ++ K W P+E R Sbjct: 239 -----LENAFNRIRQFTGDVSHELRTPLTILRGEAEVGLK-WAKEPEEFR---------- 282 Query: 290 IESVKDIADESKRMSRLVSDMLSLARADTGRTFDIEPVALEPMMTEVARRASFLPRQAEW 349 E ++ +E RMS+++ +L L+R + G ++ + L ++ E+ +++ + Sbjct: 283 -ELLRSNLEEINRMSKIIETLLELSRVEGGVKLELADLDLSDLLAELVQQSRLIAPDKSL 341 Query: 350 SVGNLGHLNGKYIVGNKDYLQQMLFIFIDNAFKYTPS-GEVAMDAVFYQNQVGIRISDTG 408 + +G I+G+ L+Q+ +DNA KYTP+ GE+++ + + I D+G Sbjct: 342 RIAFVGQ-EPVTILGDWLRLRQVFMNLLDNAVKYTPADGEISVVVDTTGDSARVAIIDSG 400 Query: 409 IGMDKDEVPHIFDRFYRADESRGITEG-IGLGLSIAKWIIDEHGGSVEVVTRQGEGTTFV 467 G+ +++PHIF+RFYR D++R +G GLGLS+ K ++ HGG +EVV+ G+G+ F Sbjct: 401 PGIPPEDLPHIFERFYRVDKARNRADGGCGLGLSLVKTFVEAHGGRIEVVSEAGKGSIFT 460 Query: 468 IWLPLLFA 475 + LP + A Sbjct: 461 VLLPRVVA 468 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 700,008,166 Number of extensions: 30114563 Number of successful extensions: 105870 Number of sequences better than 1.0e-05: 4387 Number of HSP's gapped: 98964 Number of HSP's successfully gapped: 4414 Length of query: 479 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 350 Effective length of database: 406,989,807 Effective search space: 142446432450 Effective search space used: 142446432450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)