BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0019.p (474 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00178553.1| hypothetical protein Cwat078601 [Crocosphaera... 584 e-165 gb|AAB87965.1| glutamine synthetase [Synechococcus sp. PCC 7942] 564 e-159 sp|P28605|GLNA_SYNP2 GLUTAMINE SYNTHETASE (GLUTAMATE--AMMONIA LI... 560 e-158 ref|NP_682378.1| glutamate--ammonia ligase [Thermosynechococcus ... 556 e-157 ref|NP_894434.1| Glutamine synthetase, glutamate--ammonia ligase... 552 e-156 >ref|ZP_00178553.1| hypothetical protein Cwat078601 [Crocosphaera watsonii WH 8501] Length = 474 Score = 584 bits (1506), Expect = e-165, Method: Compositional matrix adjust. Identities = 274/473 (57%), Positives = 360/473 (76%), Gaps = 6/473 (1%) Query: 4 EKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGIEE 63 ++VL+ I++NNI+ +D +F+DL G H S + +DE++F GV FDGSSI G++ I E Sbjct: 6 QEVLKMIQDNNIQIIDLKFIDLPGIWQHCSFYHNQIDEDSFDAGVPFDGSSIRGWKAINE 65 Query: 64 SDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASGVGT 122 SDM M+PDP T +IDPF PTL+++C I P GE Y RDPR IA KA E+LQ++G+G Sbjct: 66 SDMCMVPDPKTAWIDPFYEEPTLSLICSIKEPRTGEWYSRDPRTIAKKAVEYLQSTGIGD 125 Query: 123 AAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGYVPV 182 AFF PE+EFF+FDDVR++ N + Y VDS E WNT R++EGGNLA+K G K GY PV Sbjct: 126 TAFFGPEAEFFVFDDVRFDQTENKAYYSVDSVEGRWNTGREEEGGNLAYKPGYKQGYFPV 185 Query: 183 APVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYKYIV 242 +P D+ QD+R+EM +++ G+ IE+HHHEVAT GQ E+ FRF TL AD LLTYKY++ Sbjct: 186 SPTDTLQDMRTEMLLTMAKCGVPIEKHHHEVATGGQNELGFRFATLIDAADYLLTYKYVI 245 Query: 243 QNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYIGGI 302 +N A++YGK TFMPKPLF DNGSGMHVHQSI+ G PLF+E+G YA+LS++A +YIGGI Sbjct: 246 KNVAKKYGKTVTFMPKPLFNDNGSGMHVHQSIWKGGQPLFWEEGTYADLSKLATHYIGGI 305 Query: 303 LYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIEFRT 362 L HAPAL+A +NP+TNS+KRLVPG+EAPVNLV+S+GNRSA++RIP+ PK R+EFR Sbjct: 306 LKHAPALLAFSNPTTNSYKRLVPGFEAPVNLVYSQGNRSASIRIPLTGSNPKAKRLEFRC 365 Query: 363 PDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGTLEE 422 PD+T+NPYLAF+AML AG+DGIK KI P E PLD + YEL+ E+ KI S PG+LE+ Sbjct: 366 PDATSNPYLAFAAMLCAGIDGIKNKIEPAE----PLDVDFYELSPEELSKIPSTPGSLEQ 421 Query: 423 ALDSLEADYEFLTEGGVFTKDFIDNYIALK-RSEAQAVAIRVHPHEYSLYFDV 474 AL+ L D+ FLT+GGVFT+DFI N+I K +E + +R HP+E++LY+DV Sbjct: 422 ALECLGKDHAFLTDGGVFTEDFITNWIEYKLDNEVNPLRLRPHPYEFALYYDV 474 >gb|AAB87965.1| glutamine synthetase [Synechococcus sp. PCC 7942] Length = 473 Score = 564 bits (1453), Expect = e-159, Method: Compositional matrix adjust. Identities = 270/475 (56%), Positives = 354/475 (74%), Gaps = 7/475 (1%) Query: 2 SVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGI 61 + ++VLQ I+E I+ VD +F+D+ G H++L S +DE +FV GV FDGSSI G++ I Sbjct: 4 TAQEVLQLIQEKGIQLVDLKFIDMPGIWQHLTLHVSQIDENSFVEGVPFDGSSIRGWKAI 63 Query: 62 EESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTPDGER-YERDPRGIAVKAEEFLQASGV 120 ESDM M+PDP T +IDPF PTL+++C I+ P YER PR IA KA + L+ASG+ Sbjct: 64 NESDMTMVPDPTTAWIDPFMKEPTLSMICTIYEPRTNTPYERCPRAIAQKAVDCLKASGI 123 Query: 121 GTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGYV 180 G A+F PE+EFFIFDDVR++ N S Y+VDS E WN+ R++EGGNL +K K GY Sbjct: 124 GDTAYFGPEAEFFIFDDVRFDQAENKSYYYVDSIEGRWNSGREEEGGNLGYKPRYKEGYF 183 Query: 181 PVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYKY 240 PVAP D+ QD+RSEM +++ G+ IE+HHHEVAT GQ E+ FRF L + AD L+TYKY Sbjct: 184 PVAPTDTSQDMRSEMLLTMADCGVPIEKHHHEVATGGQCELGFRFGKLIEAADWLMTYKY 243 Query: 241 IVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYIG 300 +++N R+YGK TFMPK LF DNGSGMH HQSI+N PLF YA LS+MAL YIG Sbjct: 244 VIKNVGRRYGKTITFMPKLLFNDNGSGMHTHQSIWNDGQPLF-AGDQYAGLSQMALWYIG 302 Query: 301 GILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIEF 360 GIL HAPAL+ALTNP+TNS+KRLVPG+EAPVNL +S+GNRSA+VRIP++ PK R+EF Sbjct: 303 GILKHAPALLALTNPTTNSYKRLVPGFEAPVNLAYSQGNRSASVRIPLSGTNPKAKRLEF 362 Query: 361 RTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGTL 420 R PD+T+NPYLAF+AML AG+DGIK +I+ E PLD +IY+L+ E+ KI S PG+L Sbjct: 363 RCPDATSNPYLAFAAMLCAGIDGIKNQIDLGE----PLDVDIYDLSPEELAKIPSTPGSL 418 Query: 421 EEALDSLEADYEFLTEGGVFTKDFIDNYIALK-RSEAQAVAIRVHPHEYSLYFDV 474 ++AL +LEAD++FLT GGVFT+DFI N+I K +E +++R HP+E++LY+D Sbjct: 419 KDALKALEADHDFLTVGGVFTEDFIQNWIEYKLDNEVIPISLRPHPYEFALYYDC 473 >sp|P28605|GLNA_SYNP2 GLUTAMINE SYNTHETASE (GLUTAMATE--AMMONIA LIGASE) pir||A47050 glutamate-ammonia ligase (EC 6.3.1.2) - Synechococcus sp emb|CAA78366.1| glutamine synthetase [Synechococcus sp. PCC 7002] Length = 473 Score = 560 bits (1442), Expect = e-158, Method: Compositional matrix adjust. Identities = 267/471 (56%), Positives = 347/471 (73%), Gaps = 7/471 (1%) Query: 6 VLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGIEESD 65 VL+ I+E NI+ +D +FVDL G H S +DE +FV+GV FDGSSI G++ I ESD Sbjct: 8 VLRLIQEENIQIIDLKFVDLPGIWQHCSFYQDQLDEASFVDGVPFDGSSIRGWKAINESD 67 Query: 66 MVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASGVGTAA 124 M M+PDP T +IDPF TL+++C I P GE Y RDPR IA KA ++L SG+G A Sbjct: 68 MAMVPDPTTAWIDPFCKEKTLSLICSIKEPRTGEWYSRDPRSIAQKAVDYLAPSGIGDTA 127 Query: 125 FFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGYVPVAP 184 +F PE+EFF+FDDVR++ N Y+VDS E WN+ RK+ GGNLA K G K GY PV P Sbjct: 128 YFGPEAEFFVFDDVRFDQTENKGFYYVDSVEGRWNSGRKEPGGNLAHKPGYKQGYFPVPP 187 Query: 185 VDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYKYIVQN 244 D+ QD+R+EM +++ G+ IE+HHHEVAT GQ E+ FRF TL K AD L+TYKY+++N Sbjct: 188 TDTLQDMRTEMLLTMAKCGVPIEKHHHEVATGGQNELGFRFATLLKAADYLMTYKYVIKN 247 Query: 245 TARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYIGGILY 304 AR+YG+ TFMPKPLF DNGSGMH HQS++ PLF+ YANLS++AL+YIGGIL Sbjct: 248 VARKYGRTVTFMPKPLFNDNGSGMHTHQSLWKEGQPLFW-GDRYANLSQLALHYIGGILK 306 Query: 305 HAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIEFRTPD 364 HAPAL+A +NPSTNS+KRLVPG+EAPVNL +S+GNRSA+VRIP++ PK R+EFR PD Sbjct: 307 HAPALLAFSNPSTNSYKRLVPGFEAPVNLAYSQGNRSASVRIPLSGPNPKAKRLEFRCPD 366 Query: 365 STANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGTLEEAL 424 +TANPYLAF+AML AG+DGIK I+P + PLD +IY+L E+ KI S P +LE AL Sbjct: 367 ATANPYLAFAAMLCAGIDGIKNAIDPGD----PLDVDIYDLTPEELSKIPSTPASLEAAL 422 Query: 425 DSLEADYEFLTEGGVFTKDFIDNYIALK-RSEAQAVAIRVHPHEYSLYFDV 474 ++L+ D++FLT GGVFT DFI+N+I K +E + +R HP+E++LY+D Sbjct: 423 EALQQDHDFLTAGGVFTTDFIENWIEYKLDAEVNPLRLRPHPYEFALYYDC 473 >ref|NP_682378.1| glutamate--ammonia ligase [Thermosynechococcus elongatus BP-1] dbj|BAC09140.1| glutamate--ammonia ligase [Thermosynechococcus elongatus BP-1] Length = 471 Score = 556 bits (1432), Expect = e-157, Method: Compositional matrix adjust. Identities = 262/475 (55%), Positives = 353/475 (74%), Gaps = 7/475 (1%) Query: 1 MSVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRG 60 M+ + + + ++ E +D +F D+ G H+++ S + E +F GV FDGSSI G++ Sbjct: 1 MATPQEILNLIDSKYELIDLKFTDMPGTWQHLTVHKSQISESSFTEGVPFDGSSIRGWKA 60 Query: 61 IEESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASG 119 I ESDMVM+PDP T + DPF PTL+++C I P GE Y+R PR IA +A E+L+A+G Sbjct: 61 INESDMVMVPDPNTAWEDPFMKEPTLSLICTIKDPRTGELYDRCPRSIATRAIEYLKATG 120 Query: 120 VGTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGY 179 +G A+F PE+EFF+FDDVRY+ S Y++DS E VWN+ R++EGGNL +K+ K GY Sbjct: 121 IGDTAYFGPEAEFFVFDDVRYDQNQKSGYYYIDSIEGVWNSGREEEGGNLGYKIRGKEGY 180 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 PVAP D+ QD+R+EM +++ G+ IE+HHHEVATAGQ E+ FRF TL + AD L+TYK Sbjct: 181 FPVAPTDTLQDLRTEMLLTMAKCGVPIEKHHHEVATAGQCELGFRFGTLVQAADWLMTYK 240 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Y V+N AR++GKVATFMPKP+F DNGSGMH HQSI+ PLF+ G YANLS++AL YI Sbjct: 241 YCVKNVARKHGKVATFMPKPVFNDNGSGMHTHQSIWKDGQPLFWGDG-YANLSQIALWYI 299 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 GGIL HAPAL+A TNP+TNS+KRLVPG+EAPVNL +S+GNRSA+VRIP+ PK R+E Sbjct: 300 GGILKHAPALLAFTNPTTNSYKRLVPGFEAPVNLAYSQGNRSASVRIPLTGPNPKAKRLE 359 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 FR PD+T+NPYLAF+AML AG+DGIK +I+P PLD +IY+L+ E+ KI S PG+ Sbjct: 360 FRCPDATSNPYLAFAAMLCAGIDGIKNQIDPGS----PLDVDIYDLSPEELAKIPSTPGS 415 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALK-RSEAQAVAIRVHPHEYSLYFD 473 L AL++L+ D+ FLT GGVF++DFI N+I K +E +++R HP+E++LYFD Sbjct: 416 LMAALENLQKDHSFLTAGGVFSEDFILNWIQYKLDTEVIPMSLRPHPYEFALYFD 470 >ref|NP_894434.1| Glutamine synthetase, glutamate--ammonia ligase [Prochlorococcus marinus str. MIT 9313] emb|CAE20776.1| Glutamine synthetase, glutamate--ammonia ligase [Prochlorococcus marinus str. MIT 9313] Length = 473 Score = 552 bits (1423), Expect = e-156, Method: Compositional matrix adjust. Identities = 266/474 (56%), Positives = 346/474 (72%), Gaps = 5/474 (1%) Query: 2 SVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGI 61 S + VL+ IK+ IE +D +F DL G+ H+++ + +DEE F NG+AFDGSSI G++ I Sbjct: 4 SPQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVCSDLIDEEAFANGLAFDGSSIRGWKAI 63 Query: 62 EESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASGV 120 ESDM M+PD T +IDPF H TL+++C I P GE Y R PR +A KA +L +G+ Sbjct: 64 NESDMDMVPDASTAWIDPFYRHKTLSLICSIREPRSGEPYARCPRALAQKALGYLAGTGL 123 Query: 121 GTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGYV 180 AFF PE EFFIFDDVRY SG Y VD+ EA WN+ R +EGGNLA+K+ +K GY Sbjct: 124 ADTAFFGPEPEFFIFDDVRYNSGEGGCFYSVDTIEAPWNSGRIEEGGNLAYKIQLKEGYF 183 Query: 181 PVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYKY 240 PV P D+ QDIRSEM L+ + G+ +E+HHHEVA AGQ E+ +F L + ADN++ YKY Sbjct: 184 PVPPNDTAQDIRSEMLLLMGQLGIPMEKHHHEVAGAGQHELGMKFAELIEAADNVMIYKY 243 Query: 241 IVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYIG 300 IV+N AR+YGK ATFMPKP+F DNG+GMHVHQS+F G PLF+ +G YANLS+ A YIG Sbjct: 244 IVRNVARKYGKTATFMPKPVFNDNGTGMHVHQSLFKGGQPLFFGEGTYANLSQTARWYIG 303 Query: 301 GILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIEF 360 GIL HAP+ +A TNP+TNS+KRLVPG+EAPVNLV+S+GNRSAAVRIP+ PK R+EF Sbjct: 304 GILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPNPKAKRLEF 363 Query: 361 RTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGTL 420 R D+ ANPYLAFSAM+MAG+DGIK +I+P G G D++++EL +E I +VP +L Sbjct: 364 RPGDALANPYLAFSAMMMAGVDGIKNQIDP---GDG-FDEDLFELPEERLASIPTVPASL 419 Query: 421 EEALDSLEADYEFLTEGGVFTKDFIDNYIALKRSEAQAVAIRVHPHEYSLYFDV 474 AL++L AD +L EGGVFT+DFIDN+I +K E Q + R HPHE+++Y+D Sbjct: 420 NGALEALNADKNYLMEGGVFTEDFIDNWIDIKYEEVQQLRQRPHPHEFTMYYDA 473 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 739,519,908 Number of extensions: 33016952 Number of successful extensions: 80599 Number of sequences better than 1.0e-05: 1925 Number of HSP's gapped: 76738 Number of HSP's successfully gapped: 1976 Length of query: 474 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 345 Effective length of database: 406,989,807 Effective search space: 140411483415 Effective search space used: 140411483415 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)