BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0013.p (73 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAF21818.1| multiple resistance and pH regulation related pro... 72 4e-12 ref|NP_242179.1| Na+/H+ antiporter [Bacillus halodurans] >gi|253... 65 3e-10 ref|ZP_00097531.1| COG1320: Multisubunit Na+/H+ antiporter, MnhG... 65 5e-10 ref|NP_391044.1| similar to hypothetical proteins [Bacillus subt... 65 7e-10 ref|NP_694025.1| Na(+):H(+) antiporter [Oceanobacillus iheyensis... 65 7e-10 >gb|AAF21818.1| multiple resistance and pH regulation related protein G [Bacillus firmus] Length = 119 Score = 72.0 bits (175), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 34/65 (52%), Positives = 45/65 (69%) Query: 1 VLTGAFLFFYFYIEVVSIKLLIAIVFVFITSPVAGHLNGRAAYRSGVPLWKGSVQDDLKP 60 ++ FLFF+ KLL+ I+FVF+T+PVAG + GR+AYR GVPLW+ S QDDLK Sbjct: 50 IMLATFLFFFLVHGEFVGKLLLTILFVFLTAPVAGMMMGRSAYRVGVPLWEKSTQDDLKK 109 Query: 61 VLEKN 65 + EK Sbjct: 110 MYEKK 114 >ref|NP_242179.1| Na+/H+ antiporter [Bacillus halodurans] pir||A83814 Na+/H+ antiporter BH1313 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05032.1| Na+/H+ antiporter [Bacillus halodurans] Length = 117 Score = 65.5 bits (158), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 32/66 (48%), Positives = 43/66 (65%) Query: 1 VLTGAFLFFYFYIEVVSIKLLIAIVFVFITSPVAGHLNGRAAYRSGVPLWKGSVQDDLKP 60 ++ FL+F + K+L I+FVF+T+PVAG + R+AYR GVPLW SVQDDLK Sbjct: 50 IMVATFLYFLLIEGLFVGKILFTILFVFLTAPVAGLMISRSAYRIGVPLWDQSVQDDLKK 109 Query: 61 VLEKNK 66 V K + Sbjct: 110 VYAKKR 115 >ref|ZP_00097531.1| COG1320: Multisubunit Na+/H+ antiporter, MnhG subunit [Desulfitobacterium hafniense] Length = 101 Score = 65.1 bits (157), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 31/48 (64%), Positives = 38/48 (79%) Query: 17 SIKLLIAIVFVFITSPVAGHLNGRAAYRSGVPLWKGSVQDDLKPVLEK 64 SIKL + I FVF+TSPV+ H+ R+AYR+ VPL K SV+DDLK VLEK Sbjct: 53 SIKLFLGIFFVFLTSPVSSHVICRSAYRAQVPLAKASVRDDLKEVLEK 100 >ref|NP_391044.1| similar to hypothetical proteins [Bacillus subtilis] pir||E70008 conserved hypothetical protein yufB - Bacillus subtilis emb|CAB07906.1| unknown [Bacillus subtilis] emb|CAB15154.1| mrpG [Bacillus subtilis subsp. subtilis str. 168] Length = 124 Score = 64.7 bits (156), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 32/72 (44%), Positives = 46/72 (63%) Query: 1 VLTGAFLFFYFYIEVVSIKLLIAIVFVFITSPVAGHLNGRAAYRSGVPLWKGSVQDDLKP 60 +L G F + +F +S K+L+ I+F+FITSP+ GHL RAAY SGV L + SVQDD Sbjct: 51 ILLGVFFYLWFVTGELSAKILLGILFIFITSPIGGHLICRAAYNSGVKLDERSVQDDYNG 110 Query: 61 VLEKNKTKQDDS 72 + +++DS Sbjct: 111 IRNFVIKRKEDS 122 >ref|NP_694025.1| Na(+):H(+) antiporter [Oceanobacillus iheyensis HTE831] dbj|BAC15059.1| Na(+):H(+) antiporter [Oceanobacillus iheyensis HTE831] Length = 154 Score = 64.7 bits (156), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 30/72 (41%), Positives = 50/72 (69%), Gaps = 2/72 (2%) Query: 1 VLTGAFLFFYFYIEVVSIKLLIAIVFVFITSPVAGHLNGRAAYRSGVPLWKGSVQDDLKP 60 L GAF++F S++LL+ I FVF+T+PVAGHL RAAYR+ V + +++D+L+ Sbjct: 53 TLLGAFIYFASEQGFYSVRLLLGIAFVFLTAPVAGHLITRAAYRASVKMADTTIEDELRD 112 Query: 61 VLE--KNKTKQD 70 V++ + +TK++ Sbjct: 113 VIKEVQEETKEE 124 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.142 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 107,216,749 Number of extensions: 3527053 Number of successful extensions: 12857 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 12857 Number of HSP's successfully gapped: 9 Length of query: 73 Length of database: 757,237,578 Length adjustment: 44 Effective length of query: 29 Effective length of database: 637,773,222 Effective search space: 18495423438 Effective search space used: 18495423438 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 120 (50.8 bits)