BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0541.0012.p
         (97 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF 
           2,715,099 sequences; 757,237,578 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|ZP_00097532.1| COG2212: Multisubunit Na+/H+ antiporter, MnhF...    81   6e-15
ref|ZP_00183853.1| hypothetical protein Exigu073201 [Exiguobacte...    77   1e-13
ref|NP_471812.1| similar to proteins involved in resistance to c...    75   4e-13
gb|AAF21817.1| multiple resistance and pH regulation related pro...    73   2e-12
ref|NP_242180.1| Na+/H+ antiporter [Bacillus halodurans] >gi|254...    72   5e-12

>ref|ZP_00097532.1| COG2212: Multisubunit Na+/H+ antiporter, MnhF subunit
          [Desulfitobacterium hafniense]
          Length = 98

 Score = 81.3 bits (199), Expect = 6e-15,   Method: Compositional matrix adjust.
 Identities = 36/68 (52%), Positives = 53/68 (77%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
          YR ++GPT SDRV ALD +GI+++A +A+  +  RT  +F+++L+IGIL+FIGT A AR+
Sbjct: 26 YRCLKGPTASDRVQALDVLGINIIASVAIFSVLLRTTAFFEVILLIGILSFIGTIAFARF 85

Query: 82 IERDAVME 89
          IER  V+E
Sbjct: 86 IERGIVIE 93


>ref|ZP_00183853.1| hypothetical protein Exigu073201 [Exiguobacterium sp. 255-15]
          Length = 94

 Score = 77.4 bits (189), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 32/69 (46%), Positives = 54/69 (78%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
          YR+I+GP+ +DRV ALD+IGI L++V+ ++ +  RT +Y +++L+IGIL FIGT A +++
Sbjct: 22 YRVIKGPSVADRVIALDSIGISLISVVGLVSIILRTSNYLEVILLIGILAFIGTVAFSKF 81

Query: 82 IERDAVMEH 90
          IE+  ++E 
Sbjct: 82 IEKGEIIER 90


>ref|NP_471812.1| similar to proteins involved in resistance to cholate and to
          NA(+) and in pH homeostasis [Listeria innocua]
 ref|NP_465906.1| similar to proteins involved in resistance to cholate and to
          NA(+) and in pH homeostasis [Listeria monocytogenes
          EGD-e]
 pir||AE1742 protein involved in resistance to cholate and to NA(+) and in pH
          homeostasis homolog lin2482 [imported] - Listeria
          innocua (strain Clip11262)
 pir||AG1372 proteins involved in resistance to cholate and to NA(+) and in pH
          homeostasis homolog lmo2383 [imported] - Listeria
          monocytogenes (strain EGD-e)
 emb|CAD00461.1| lmo2383 [Listeria monocytogenes]
 emb|CAC97709.1| lin2482 [Listeria innocua]
          Length = 95

 Score = 75.1 bits (183), Expect = 4e-13,   Method: Compositional matrix adjust.
 Identities = 32/73 (43%), Positives = 54/73 (73%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
          YR+++GPT SD+V ALD+IG++L+A++A+L M   T  + D++L+I +L FIGT + A++
Sbjct: 23 YRILKGPTTSDKVVALDSIGMNLVAIVALLSMFYDTHAFLDVILLIALLAFIGTVSFAKF 82

Query: 82 IERDAVMEHGGDK 94
          IE+  V++   D 
Sbjct: 83 IEKGKVIDRERDN 95


>gb|AAF21817.1| multiple resistance and pH regulation related protein F [Bacillus
          firmus]
          Length = 91

 Score = 72.8 bits (177), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 36/69 (52%), Positives = 48/69 (69%)

Query: 23 RLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARYI 82
          R + GPT SDR+ ALDT GI+L+  I V+ M Q T  Y ++VLVI IL FIG+ AL+++I
Sbjct: 23 RTLIGPTMSDRIVALDTFGINLIGFIGVIMMLQETLAYSEVVLVISILAFIGSIALSKFI 82

Query: 83 ERDAVMEHG 91
          ER  V + G
Sbjct: 83 ERGVVFDRG 91


>ref|NP_242180.1| Na+/H+ antiporter [Bacillus halodurans]
 pir||B83814 Na+/H+ antiporter BH1314 [imported] - Bacillus halodurans
          (strain C-125)
 dbj|BAB05033.1| Na+/H+ antiporter [Bacillus halodurans]
          Length = 95

 Score = 71.6 bits (174), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 33/72 (45%), Positives = 50/72 (69%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
          YR I GPT SDR+ ALDT GI+L+  I ++ + Q T  Y +++LV+GIL F+G+ ALA++
Sbjct: 22 YRTIVGPTMSDRIVALDTFGINLIGFIGIIMILQETIAYAEVILVLGILAFVGSIALAKF 81

Query: 82 IERDAVMEHGGD 93
          +ER  + +   D
Sbjct: 82 LERGTIFDRSRD 93


  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Mar 24, 2004  1:53 AM
  Number of letters in database: 757,237,578
  Number of sequences in database:  2,715,099
  
Lambda     K      H
   0.328    0.144    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2715099
Number of Hits to DB: 99,282,715
Number of extensions: 2968410
Number of successful extensions: 10538
Number of sequences better than 1.0e-05: 46
Number of HSP's gapped: 10537
Number of HSP's successfully gapped: 46
Length of query: 97
Length of database: 757,237,578
Length adjustment: 65
Effective length of query: 32
Effective length of database: 580,756,143
Effective search space: 18584196576
Effective search space used: 18584196576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 120 (50.8 bits)