BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0003.p (377 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAN17729.1| glycerol dehydrogenase DhaD1 [Clostridium butyricum] 559 e-158 ref|NP_561015.1| glycerol dehydrogenase [Clostridium perfringens... 535 e-151 ref|NP_815079.1| glycerol dehydrogenase, putative [Enterococcus ... 498 e-140 ref|NP_796742.1| glycerol dehydrogenase GldH [Vibrio parahaemoly... 328 1e-88 ref|NP_931305.1| glycerol dehydrogenase (GLDH) [Photorhabdus lum... 322 1e-86 >gb|AAN17729.1| glycerol dehydrogenase DhaD1 [Clostridium butyricum] Length = 380 Score = 559 bits (1441), Expect = e-158, Method: Compositional matrix adjust. Identities = 267/376 (71%), Positives = 307/376 (81%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGED++LNLGYFV++FG+SALLIAH DDV RVK KL+ T KF I+ + Sbjct: 1 MRKAFICPTKYVQGEDDILNLGYFVRSFGESALLIAHKDDVTRVKDKLNKTCEKFKISFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 F+GECSR+EV RLQ +A E+ C+C IGLGGGKAIDTAKCVAEG+ALIIVPTIAATDA Sbjct: 61 ESGFKGECSREEVCRLQQIASENKCSCIIGLGGGKAIDTAKCVAEGEALIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYT EGAFDDYAYFKQSPSVV+IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTNEGAFDDYAYFKQSPSVVLIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + S+SNVNAGLPCG REGV EAKGT ALALA++CYETL+ DG KAK+A D N+VTPAL Sbjct: 181 SNSYSNVNAGLPCGFREGVCNEAKGTNTALALAKLCYETLINDGAKAKVASDCNIVTPAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIE NI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLEN+ Sbjct: 241 ENIIEANILLSGIGFESGGLAGAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENSPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 E+ +VL FC VGLPVCLADIGV SI EL+EVA+KAC EESIHSMPFP++ E VAA Sbjct: 301 EELNQVLDFCLEVGLPVCLADIGVESINENELMEVAKKACYKEESIHSMPFPISAEDVAA 360 Query: 361 AITVADQLGHAFKKGK 376 +I AD++G +K K Sbjct: 361 SIIAADKIGREYKLKK 376 >ref|NP_561015.1| glycerol dehydrogenase [Clostridium perfringens] dbj|BAB79805.1| glycerol dehydrogenase [Clostridium perfringens str. 13] Length = 376 Score = 535 bits (1377), Expect = e-151, Method: Compositional matrix adjust. Identities = 256/376 (68%), Positives = 302/376 (80%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGE+ELLNLGYFV+T+G SALLIAHP+D++RVK KLDAT+ KF I + Sbjct: 1 MRKAFICPTKYVQGENELLNLGYFVETYGKSALLIAHPEDIKRVKDKLDATAKKFNIEFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 G F+GECSRQEV RLQ LA+E C CT+GLGGGKA+DTAKCVAEG+ LI+VPTIAATDA Sbjct: 61 EGGFKGECSRQEVKRLQDLAKEMKCDCTVGLGGGKALDTAKCVAEGEKLIVVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYT +G+FDDYAYFK SPSVV++DT +IA+APTRFLV+GMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTEDGSFDDYAYFKASPSVVLVDTEVIAKAPTRFLVAGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SF+ VNAGLPCGVRE + A GT A+LALA++CYETLLEDG KAK ACD N VT +L Sbjct: 181 SSSFTKVNAGLPCGVREEKCRPAIGTNASLALAKLCYETLLEDGKKAKDACDCNCVTKSL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENI+ETNI HAIH+GLT+LEG+H Y+HGEKVAF +AQLVLENA Sbjct: 241 ENIVETNILLSGLGFESGGLAAAHAIHDGLTILEGTHGYFHGEKVAFGIIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 E+ EVL FC +GLPVCL DIGV ++ EEL EVA KACIPEESI+SMPF + V + Sbjct: 301 EELYEVLDFCLEIGLPVCLEDIGVTEVSDEELFEVAEKACIPEESIYSMPFGIEVSEVVS 360 Query: 361 AITVADQLGHAFKKGK 376 AI +AD++G +K K Sbjct: 361 AIKLADKIGKDYKNRK 376 >ref|NP_815079.1| glycerol dehydrogenase, putative [Enterococcus faecalis V583] gb|AAO81149.1| glycerol dehydrogenase, putative [Enterococcus faecalis V583] Length = 379 Score = 498 bits (1283), Expect = e-140, Method: Compositional matrix adjust. Identities = 243/373 (65%), Positives = 287/373 (76%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK I PTKY+QGEDELLNLGYFV TFG ALLIA+P DV RV+ +L+ T+ KF IT + Sbjct: 1 MRKALILPTKYVQGEDELLNLGYFVSTFGKKALLIANPADVDRVRPQLEETAKKFDITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 G F GE +R+E RLQ + ++ C IGLGGGKAID +K VAEG+ LIIVPTIAA DA Sbjct: 61 EGGFSGEVTREETKRLQNVGQKEQADCIIGLGGGKAIDASKVVAEGENLIIVPTIAAQDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLY +G+FDDYAYFKQSPSVVM+DT ++A+APTRFLV+GMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYHEDGSFDDYAYFKQSPSVVMVDTKVVAKAPTRFLVAGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 S+S VNA LP G REG++ A GT AALA+A++C+E L EDG+KAKLACD NVVT +L Sbjct: 181 HNSYSRVNASLPMGSREGLTPAASGTYAALAMAKLCWENLQEDGLKAKLACDANVVTESL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 IIETNI HAIHNG+TVL G+H Y HGEKVAF+T+AQLVLE+A Sbjct: 241 NKIIETNILLSGLGFESGGLAAAHAIHNGMTVLPGAHSYLHGEKVAFATIAQLVLEDAAK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 E++ VL F ASVGLP+ L+DIGV IT EE LEVA+ ACIPEESIHSMPFP+ E VA Sbjct: 301 DELEAVLDFSASVGLPLTLSDIGVDEITYEEALEVAKIACIPEESIHSMPFPIVVEEVAN 360 Query: 361 AITVADQLGHAFK 373 AI VAD++G +K Sbjct: 361 AIIVADKVGQEYK 373 >ref|NP_796742.1| glycerol dehydrogenase GldH [Vibrio parahaemolyticus RIMD 2210633] dbj|BAC58626.1| glycerol dehydrogenase GldH [Vibrio parahaemolyticus] Length = 360 Score = 328 bits (841), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 187/381 (49%), Positives = 233/381 (61%), Gaps = 32/381 (8%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHP-------DDVQRVKA--KLDAT 51 M K+ ISP+KY+QGE L ++ ++VKT G+ L+IA DDV++ A KL T Sbjct: 1 MDKIIISPSKYVQGEQVLTSIAHYVKTLGERPLVIADEFVTNLVGDDVKQSFADEKLPLT 60 Query: 52 SSKFGITLITGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVA--EGDAL 109 + FG GECSR E+ R+ + +G+GGGK +DTAK VA + Sbjct: 61 MNIFG---------GECSRIEIERITDICATQKHDVIVGIGGGKTLDTAKAVAFYTKIPV 111 Query: 110 IIVPTIAATDAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGD 169 ++VPTIA+TDAPTS AV+YTPEG F +Y ++P +V++DT++IA+AP R LVSGMGD Sbjct: 112 VVVPTIASTDAPTSALAVIYTPEGEFAEYLMIPKNPDMVIMDTSVIAKAPVRLLVSGMGD 171 Query: 170 ALSTYFEARATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKL 229 ALSTYFEARA S AG T++A ALA++CYETLLEDG+KAK Sbjct: 172 ALSTYFEARANMTSGKATMAG------------GLATRSAQALAKLCYETLLEDGLKAKA 219 Query: 230 ACDQNVVTPALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFST 289 A + V T A+ENIIE N HAIHNGLT LE HH YHGEKVAF T Sbjct: 220 AVENGVSTKAVENIIEANTYLSGIGFESSGLAGAHAIHNGLTKLEECHHLYHGEKVAFGT 279 Query: 290 LAQLVLENADSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSM 349 L QLVLENA EI VLAFC SVGLP L D+GV + +L EVA + E+IH+M Sbjct: 280 LVQLVLENAAMEEINTVLAFCRSVGLPTNLFDMGVKELNHAKLREVAEASTAEGETIHNM 339 Query: 350 PFPVTPEAVAAAITVADQLGH 370 PFPVT E V +AI A QLG Sbjct: 340 PFPVTAENVYSAILTAHQLGQ 360 >ref|NP_931305.1| glycerol dehydrogenase (GLDH) [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE16487.1| glycerol dehydrogenase (GLDH) [Photorhabdus luminescens subsp. laumondii TTO1] Length = 366 Score = 322 bits (824), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 169/376 (44%), Positives = 236/376 (62%), Gaps = 16/376 (4%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M K+ SP+KYIQG D LL++G + KT D L+I ++ V + ++ ++ IT Sbjct: 1 MLKIIQSPSKYIQGPDALLHIGQYTKTIADHVLVIIGNSAMKLVGDTVHSSLEQYEITRH 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDAL--IIVPTIAAT 118 F GECSR E+ RL + ++H C IG+GGGK DTAK VA L +I PTIA+T Sbjct: 61 FEVFGGECSRNEIKRLSDILKKHECQAVIGIGGGKVQDTAKAVAYESRLPVLIAPTIAST 120 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 +APTS +V+Y+ +G ++DY + +P +V+IDT+IIA+APTRFL++G+GDAL+TYFEAR Sbjct: 121 NAPTSALSVIYSDDGKYEDYLFLPTNPDIVLIDTSIIAKAPTRFLIAGIGDALATYFEAR 180 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 + P G+S + A+ALA +CYETLLE+G KAKLA + +V TP Sbjct: 181 ICGAGYK------PRMTEGGIS------RTAVALAHLCYETLLEEGYKAKLAVETDVSTP 228 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 A+EN+IE H++HNG TVLE HH YHGEKVAF L QL+LEN+ Sbjct: 229 AVENVIEAITYLSGLGFENTGVAAAHSVHNGFTVLEECHHMYHGEKVAFGILVQLILENS 288 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHS--ITREELLEVARKACIPEESIHSMPFPVTPE 356 + E+ +L FC +GLPV L +GV +E+++ VA +C +E+IH+MPF VTPE Sbjct: 289 PNEELDTLLDFCVQMGLPVTLDQLGVKDGEKLKEKVMAVATASCAEDETIHNMPFEVTPE 348 Query: 357 AVAAAITVADQLGHAF 372 V AAI AD++G + Sbjct: 349 KVYAAIMAADRMGRDW 364 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 496,798,251 Number of extensions: 18604945 Number of successful extensions: 46159 Number of sequences better than 1.0e-05: 131 Number of HSP's gapped: 45889 Number of HSP's successfully gapped: 135 Length of query: 377 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 250 Effective length of database: 412,420,005 Effective search space: 103105001250 Effective search space used: 103105001250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)