BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0011.p (438 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAS55729.1| mannose-1-phosphate guanylyltransferase [Aneurini... 296 8e-79 ref|ZP_00038029.1| COG0836: Mannose-1-phosphate guanylyltransfer... 258 1e-67 ref|NP_069926.1| mannose-6-phosphate isomerase/mannose-1-phospha... 112 2e-23 ref|NP_578318.1| mannose-6-phosphate isomerase/mannose-1-phospha... 109 2e-22 ref|ZP_00149223.1| hypothetical protein Mbur022101 [Methanococco... 105 2e-21 >gb|AAS55729.1| mannose-1-phosphate guanylyltransferase [Aneurinibacillus thermoaerophilus] Length = 464 Score = 296 bits (757), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 160/439 (36%), Positives = 238/439 (54%), Gaps = 7/439 (1%) Query: 4 ILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 ILL GGSG++ RSK FL++L + ESM+ RV QL GL + + Sbjct: 4 ILLSGGSGKRLWPLSNDSRSKQFLKVLKNDNNEFESMVQRVWNQLHTVGLAEDSYVATSK 63 Query: 63 DQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYTDDA 122 Q+ + P+I EP +R TF + +D + V P D Y + Sbjct: 64 SQVDMLHAQLGTEVPLIIEPERRDTFPAIALAATYLYSMEGVREDDVISVLPVDPYVESR 123 Query: 123 FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVLS---FQEKPD 179 FFD L S AEL L+G +PT PS++YGYI+P A VL+ F EKP Sbjct: 124 FFDRVKDLETALVQSSAELALIGVKPTFPSEKYGYIVPKKGEHDVVANVLAVDYFVEKPS 183 Query: 180 SARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAE 239 ++RA EL++QQA WNCGIFAF+L +++S L+ LP + Y P SFD EV E Sbjct: 184 TSRAQELMKQQAFWNCGIFAFKLGYLISLLKEKKLPTDYEEMLHTYNQLPKISFDYEVVE 243 Query: 240 RSRKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVS 299 +++ ++L +EGEW+DLG+W+ LT + ++G+G I +TH++N+L IP+ ++G+S Sbjct: 244 KAQNIIMLPYEGEWKDLGTWNTLTEEMNTNLLGKGIISEGCENTHLINELDIPVTILGIS 303 Query: 300 GIVAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRTF--SGPELILT 357 V A SP+GIL+ K + IK+I++ + P Y E WG Y VLD T +G E +LT Sbjct: 304 NAVIAASPDGILVTDKEASPRIKDIMKNFNQRPMYEERRWGWYRVLDHTKVENGNE-VLT 362 Query: 358 LALSLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAF 417 + + G +L S+ W I+SG+G+ VLN + G + I G +H IRA Sbjct: 363 KRIGVHAGKNLSYQIHHKRSEVWTIISGEGQFVLNDQLYHVKPGDVLQIPVGAKHAIRAI 422 Query: 418 TALKLIEIRTCGQDDEQKI 436 T L+ +E++T + E+ I Sbjct: 423 TDLQFVEVQTGSELIEEDI 441 >ref|ZP_00038029.1| COG0836: Mannose-1-phosphate guanylyltransferase [Enterococcus faecium] Length = 437 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 142/440 (32%), Positives = 234/440 (53%), Gaps = 13/440 (2%) Query: 1 VHTILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFV 59 ++ +LL GGSG++ RSK F+++ DG ESM+ RV RQ+ A+ Sbjct: 1 MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDG-YESMVQRVYRQIKKVDKDATVTIA 59 Query: 60 AHEDQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYT 119 + Q++ H V EP +R TF PE+++ V P D Y Sbjct: 60 TSKTQVSAIHNHLGEDVCVSVEPCRRDTFPAIALATAYLVDVMGVNPEESVVVCPVDPYV 119 Query: 120 DDAFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVLSFQEKPD 179 +D +F+ +D +A LVL+G PT+PS++YGYIIP +A A+ V +F+EKP Sbjct: 120 EDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIPESAKQTAF--VNTFKEKPT 177 Query: 180 SARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAE 239 A A E I Q ALWN G+FA++L +++ ++ + Y SFD V E Sbjct: 178 EAVAKEYITQGALWNGGVFAYKLKYVMERAHQLIDFVDYQDLFNKYETLTKISFDYAVVE 237 Query: 240 RSRKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVS 299 K V R G W+DLG+W+ LT + + ++G+G + + HI+N+L +P+ +G+ Sbjct: 238 HEDKIQVQRFAGMWKDLGTWNTLTEAMEESIVGKGVMNENCDGVHILNELAVPVLAMGLH 297 Query: 300 GIVAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRTFSGPELILTLA 359 +V + SPEGIL++ K +++ IK + Q ++ + E WGSY V+D +T+ Sbjct: 298 DVVISASPEGILVSDKEQSSYIKPFVDQFEQQIMFAEKSWGSYKVMDVETES----MTIK 353 Query: 360 LSLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAFTA 419 ++L GH + ++ + WV+++G G+ +++G A + G + T++ G RH + A T Sbjct: 354 VTLNAGHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQAVKAGDVITMSAGCRHTVLADTE 413 Query: 420 LKLIEIRTCGQD----DEQK 435 LKLIE++ G+D D+QK Sbjct: 414 LKLIEVQ-LGKDINVKDKQK 432 >ref|NP_069926.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) [Archaeoglobus fulgidus DSM 4304] pir||H69386 mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) homolog - Archaeoglobus fulgidus gb|AAB90149.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) [Archaeoglobus fulgidus DSM 4304] Length = 448 Score = 112 bits (279), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 90/339 (26%), Positives = 149/339 (43%), Gaps = 30/339 (8%) Query: 107 EDTLCVAPAD--MYTDDAFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAG 164 E V P+D + D+ + + F + S+ +V G PT P YGYI PG Sbjct: 101 EGKFAVLPSDHLIKADEEYLNAFRSAEKL---SENYIVTFGITPTRPHTGYGYIKPGKEL 157 Query: 165 PGAYAPVLSFQEKPDSARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTE- 223 G + V F+EKP A E + + LWN G+F F + L+ + P E Sbjct: 158 EGGFE-VEQFKEKPSRELAEEYVSKGYLWNSGMFVFDSKVFVEELKELA-PEFAKVLEEG 215 Query: 224 --LYPGFPVRSFDQEVAERSRKAVVLRHEGEWRDLGSWDALTARL-----ADPVIGEGGI 276 Y P SFD + E+S + V+ + W DLG++D++ + + + E I Sbjct: 216 EEAYKQIPEASFDYAILEKSGRVAVVPIKTFWSDLGNFDSIYEVMEKDERGNAIKSESCI 275 Query: 277 WGSSRDTHIVNDLGIPLHVIGVSGIVAAGSPEGILIAGKGEANAIKEILQ-------QAA 329 S + ++ L IG+ ++ + + +L+A +GEA ++E+ + +A Sbjct: 276 PVDSENNLVITQRLTAL--IGLRDLIVIDTDDALLVARRGEAEKVREVYRLLAEKGDKAV 333 Query: 330 EEPRYGETGWGSYTVLDRTFSGPELILTLALSLLPGHDLPELQCQHSSKSWVIVSGQGEI 389 E R WGSYTVL+ S +++ P L + H S+ WV+V G I Sbjct: 334 EVHRTAHRPWGSYTVLEENKSYK----IKRITVKPKKRLSLQRHYHRSEHWVVVKGTARI 389 Query: 390 VLNGLPAAAQTGGIFTIARGDRHGIR--AFTALKLIEIR 426 V++G ++G + G H I L++IEI+ Sbjct: 390 VVDGNEILLRSGESTFVPAGAIHRIENPGKIPLEIIEIQ 428 >ref|NP_578318.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase [Pyrococcus furiosus DSM 3638] gb|AAL80713.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase; (manC) [Pyrococcus furiosus DSM 3638] Length = 461 Score = 109 bits (272), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 87/302 (28%), Positives = 132/302 (43%), Gaps = 30/302 (9%) Query: 110 LCVAPADMYTD--DAFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAGPGA 167 + V P+D D + + + F + + LA++ LV G +PT P YGYI PG Sbjct: 109 VAVLPSDHAIDVNENYIEAFKK-AEKLAENY--LVTFGIKPTKPHTGYGYIKPGEKISNL 165 Query: 168 YAPVLSFQEKPDSARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAF------ 221 V F+EKPD A ++ WN G+F FR + + R P AF Sbjct: 166 GFLVDEFKEKPDLETAKRYVENGYYWNSGMFMFRTTLFMEEA-RKHAPDVVKAFEEGKTI 224 Query: 222 TELYPGFPVRSFDQEVAERSRKAVVLRHEGEWRDLGSWDALTARLADPVIGEG----GIW 277 E Y P S D + E++ KA V+ W DLGS+DA+ L G G Sbjct: 225 EEAYELAPEISVDYGIMEKTDKAAVVPLNTYWNDLGSFDAVYEALQKDENGNAVEVRGFK 284 Query: 278 GSSRDTHIVNDLGIPLHV---IGVSGIVAAGSPEGILIAGKGEANAIKEILQQAAEEP-- 332 + N+L + + +GV ++ + + +L+A KGE +KE+ ++ EE Sbjct: 285 AKYINVDSKNNLILTERLTATVGVEDLIIVDTGDALLVAKKGETQKVKEVYKKLKEENDE 344 Query: 333 -----RYGETGWGSYTVLDRTFSGPELILTLALSLLPGHDLPELQCQHSSKSWVIVSGQG 387 R WGSYTVL+ E +++LPG L H S+ WV+V G Sbjct: 345 RAIVHRTAYRPWGSYTVLEEG----ERYKIKRITVLPGKRLSLQLHYHRSEHWVVVRGTA 400 Query: 388 EI 389 ++ Sbjct: 401 KV 402 >ref|ZP_00149223.1| hypothetical protein Mbur022101 [Methanococcoides burtonii DSM 6242] Length = 416 Score = 105 bits (263), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 84/323 (26%), Positives = 140/323 (43%), Gaps = 36/323 (11%) Query: 141 LVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVLSFQEKPDSARALELIQQQALWNCGIFAF 200 LV G P+ P YGYI PGN G VL F+EKPD A ++Q LWN G+F F Sbjct: 83 LVTFGVVPSSPHTGYGYIHPGN-DIGFGNEVLEFKEKPDGEIAKMYVKQGYLWNSGMFLF 141 Query: 201 RLSFMLSHLERMGLPAEPAAF-------------TELYPGFPVRSFDQEVAERSRKAVVL 247 + + EP+ F +Y P S D + ERS K V+ Sbjct: 142 DTDIFFEEVRKY----EPSMFDAFCGDEGVIRNIVSIYSSLPSISIDYGIMERSNKVAVV 197 Query: 248 RHEGEWRDLGSWDALTARL-----ADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVSGIV 302 + E +W DLG++DA+ ++ V + SS + + + +I ++ +V Sbjct: 198 KLEHKWSDLGNFDAIYGESDKDDHSNAVYDCDSNFISSNGNLVYSKPDKIVSLIDINDMV 257 Query: 303 AAGSPEGILIAGKGEANAIKEILQ----QAAEEPRYGET---GWGSYTVLDRTFSGPELI 355 +P+ +L+ + + +K+++ + E +T WG+YT+L+ T E Sbjct: 258 VVDTPDALLVCPRKSSQKVKDVVNSLSVEGDERISLHQTVYRPWGTYTILEST----ERH 313 Query: 356 LTLALSLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIR 415 ++++P L H S+ WV+V G + + G + G I GD+H + Sbjct: 314 KIKNITVMPQKKLSYQLHHHRSEHWVVVEGIACVQIEGKHFYLRQGESTFIRPGDKHRLL 373 Query: 416 --AFTALKLIEIRTCGQDDEQKI 436 L++IE++ DE I Sbjct: 374 NPGKLPLEIIEVQLGESVDEDDI 396 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 655,549,666 Number of extensions: 27728544 Number of successful extensions: 61125 Number of sequences better than 1.0e-05: 274 Number of HSP's gapped: 60742 Number of HSP's successfully gapped: 275 Length of query: 438 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 309 Effective length of database: 406,989,807 Effective search space: 125759850363 Effective search space used: 125759850363 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)