BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0004.p (196 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_622201.1| conserved hypothetical protein [Thermoanaerobac... 124 8e-28 ref|NP_782946.1| putative membrane-associated alkaline phosphata... 102 4e-21 ref|NP_349312.1| Uncharacterized conserved membrane protein, Ded... 101 1e-20 ref|NP_561075.1| hypothetical protein [Clostridium perfringens] ... 101 1e-20 ref|NP_971611.1| membrane protein, putative [Treponema denticola... 83 4e-15 >ref|NP_622201.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM23805.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 223 Score = 124 bits (312), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 72/183 (39%), Positives = 111/183 (60%) Query: 4 DEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTG 63 ++FR +I S G G + I Q+ Q V+ IPGEVVQ+AGGY+YG LGT+Y G+ G Sbjct: 37 EKFRNWINSFGSLGVLIFISIQVFQVVVFVIPGEVVQVAGGYLYGTILGTLYSVIGITLG 96 Query: 64 SAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAG 123 S I F R +G + ++ ++KLK ++ + LF+ F +PG+PKD L YIAG Sbjct: 97 SLICFSIARILGYDFVKNIVSEEKLKKFDYIINNPKGEIVLFLLFFLPGMPKDALSYIAG 156 Query: 124 LTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKL 183 +TP+K F I L RLP + S +G+NL KNY +++++A+V F+ G+ +D + Sbjct: 157 ITPVKFYNFFIITLFARLPGIFFSAYIGSNLGSKNYLMAGLVAIVALVLFLAGVYKRDVI 216 Query: 184 IQR 186 I++ Sbjct: 217 IEK 219 >ref|NP_782946.1| putative membrane-associated alkaline phosphatase [Clostridium tetani E88] gb|AAO36883.1| putative membrane-associated alkaline phosphatase [Clostridium tetani E88] Length = 241 Score = 102 bits (255), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 56/183 (30%), Positives = 100/183 (54%) Query: 4 DEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTG 63 +E ++ I+S G I Q++Q V IPGE++QIAGGYI+ G+++ G+ G Sbjct: 47 EEIKRIIMSYGHNSIIAFIALQVIQVVFFFIPGEIIQIAGGYIFDAFYGSLFSIIGITIG 106 Query: 64 SAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAG 123 I F +RF G + ++I K +K+ + ++ + +F+ +LIPG+PKD+L YI G Sbjct: 107 GGIVFSISRFFGKPFVEKIISNKHIKFFSKVLNSDKINYIVFILYLIPGIPKDVLAYICG 166 Query: 124 LTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKL 183 ++ +K GRLP ++ S G N+ KN + +S++ V+ ++G++ + Sbjct: 167 ISNVKFKDFLIYSSLGRLPGIVISTYFGQNISSKNIPVLVGVSIVTVILIIIGVVKGRSI 226 Query: 184 IQR 186 I Sbjct: 227 INN 229 >ref|NP_349312.1| Uncharacterized conserved membrane protein, DedA family [Clostridium acetobutylicum] pir||A97233 uncharacterized conserved membrane protein, DedA family CAC2706 [imported] - Clostridium acetobutylicum gb|AAK80652.1| Uncharacterized conserved membrane protein, DedA family [Clostridium acetobutylicum] Length = 236 Score = 101 bits (251), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 59/176 (33%), Positives = 97/176 (55%), Gaps = 1/176 (0%) Query: 7 RKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTGSAI 66 +++ILS GK G + Q+LQ V IPGE++QIAGGYI+G GT+ G+ GSA+ Sbjct: 47 KEFILSYGKFGFFAFFVMQMLQVVAFFIPGEIIQIAGGYIFGTFWGTVISLLGITAGSAV 106 Query: 67 AFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAGLTP 126 AFY + +G + ++ K+ + + ++ + + +L+PGLPKD+L Y+ G+T Sbjct: 107 AFYLSNKLGRPFVKKISGKQNISFFEKLIKNGEIKYVITLIYLVPGLPKDILAYVCGITD 166 Query: 127 MKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDK 182 + GR P + S G + N +I++V++VV F LG ++K K Sbjct: 167 LSLKDFIIYSTIGRFPGIFISAYFGNTITHGNIKMMVIIAVISVVLFGLG-VFKGK 221 >ref|NP_561075.1| hypothetical protein [Clostridium perfringens] dbj|BAB79865.1| hypothetical protein [Clostridium perfringens str. 13] Length = 197 Score = 101 bits (251), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 63/179 (35%), Positives = 98/179 (54%) Query: 7 RKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTGSAI 66 + Y+ G + +FQILQ VI IPGEV+Q AGGYI+G GT+ G+ GSAI Sbjct: 14 KSYLERFGPWAAVIFFVFQILQVVIFFIPGEVIQAAGGYIFGALGGTLLSFFGIAVGSAI 73 Query: 67 AFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAGLTP 126 FY + G L+ R + K + ++ F + L + F +PG PKD L+ + L+ Sbjct: 74 LFYICQKFGKKLVQRFVPKDMYDKLEKILNSPKFKLILVILFFLPGAPKDSLIMVCALSN 133 Query: 127 MKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKLIQ 185 + + GR+P L+AS +GANL + N II+ V+A++A LG+I++ + + Sbjct: 134 ITIGHFIIFSMIGRIPALVASSFMGANLAEGNIVGVIIIGVIALIACALGVIFRKDVFK 192 >ref|NP_971611.1| membrane protein, putative [Treponema denticola ATCC 35405] gb|AAS11492.1| membrane protein, putative [Treponema denticola ATCC 35405] Length = 230 Score = 82.8 bits (203), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 51/160 (31%), Positives = 81/160 (50%) Query: 27 LQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTGSAIAFYFTRFMGGFLIGRLIEKK 86 LQ V+A IPG +I GY +G G + AG+ + F F + G I + Sbjct: 68 LQVVVAVIPGCPFEIGAGYAFGAVEGALVSLAGISAAGILIFLFVKKFGKAAIEIFFSPE 127 Query: 87 KLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAGLTPMKPLRLFGILLAGRLPWLIA 146 K++ + +K + +F+ FLIPG PKDLL Y+AGLTP++ + R P ++A Sbjct: 128 KIESIKIFKNKKRLHLIVFIVFLIPGTPKDLLTYMAGLTPIRLSEWLILSTLARFPAILA 187 Query: 147 SVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKLIQR 186 S G +L QKN + + ++ +A G++ +KL R Sbjct: 188 STIAGTSLAQKNTGLALAVFLIGSIAAFAGVLIFNKLNSR 227 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.147 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 286,239,635 Number of extensions: 11715631 Number of successful extensions: 45044 Number of sequences better than 1.0e-05: 87 Number of HSP's gapped: 45145 Number of HSP's successfully gapped: 87 Length of query: 196 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 77 Effective length of database: 434,140,797 Effective search space: 33428841369 Effective search space used: 33428841369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 122 (51.6 bits)