BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0029.p (291 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00079954.1| COG2159: Predicted metal-dependent hydrolase ... 72 1e-11 ref|ZP_00188560.1| hypothetical protein Rxyl038301 [Rubrobacter ... 70 6e-11 ref|NP_949534.1| Amidohydrolase 2 [Rhodopseudomonas palustris CG... 69 1e-10 ref|NP_343501.1| Conserved hypothetical protein [Sulfolobus solf... 69 2e-10 ref|NP_774189.1| blr7549 [Bradyrhizobium japonicum] >gi|27355832... 68 2e-10 >ref|ZP_00079954.1| COG2159: Predicted metal-dependent hydrolase of the TIM-barrel fold [Geobacter metallireducens] Length = 286 Score = 72.0 bits (175), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 65/228 (28%), Positives = 104/228 (45%), Gaps = 29/228 (12%) Query: 63 SPNPMGLDLEEEVPPF------LMECVGINPNRLAGAQAQAELDRIEARMQAPEVAGIKL 116 P G+ E+V F L+ INP+ EL R+ ++ G+KL Sbjct: 77 CPITTGICSNEQVEAFCKGRKRLIPFCSINPH--LTTDLGGELRRL---VETEGFRGLKL 131 Query: 117 YAGYYHYYVHDKIYAPVYELAAKYSLPVVIHTGDTYSMNGLLKYSHPLTVDELAYQQRGV 176 Y Y HYY++D P+Y+ A + +PV+IHTG + +KY P+ +D++A + Sbjct: 132 YPSYQHYYLNDPRVYPLYQAAQELGIPVLIHTGSSVFKGTRIKYCDPIHLDDVANDFPSL 191 Query: 177 NFMICHLGDPWVMDAAEVVAK-NPNVYADLSGLVVGDRPHFERFMNEPLFMDHFRRALVY 235 N ++ H G + D A ++K + N+Y ++SGL M +F Sbjct: 192 NLVMAHSGRGFWYDRAFFLSKLHSNLYMEVSGLPPSK------------LMTYFPELARN 239 Query: 236 CDHYEKMLFGTDWPLAP-IGLYAEFIRRL-VPGQHHEKVFYRNAFELF 281 D K++FG+DWP A I + I L +P + EK+ NA L Sbjct: 240 TD---KVIFGSDWPGASQIKRNMDAIGALPLPAEGVEKILGGNAARLL 284 >ref|ZP_00188560.1| hypothetical protein Rxyl038301 [Rubrobacter xylanophilus DSM 9941] Length = 286 Score = 70.1 bits (170), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 57/193 (29%), Positives = 89/193 (46%), Gaps = 26/193 (13%) Query: 64 PNPMGLDLEEEVPPFLMECVGINPNRLAGAQAQAELDRIEARMQAPEVAGIKLYAGYYHY 123 PN L+ +E P L+ I+P R A +A R+ V G K + + Sbjct: 75 PNEEVLEAAKENPDVLIPFASIHPARGAMGVKEAR------RLIEMGVRGFKFHPNLQDF 128 Query: 124 YVHDKIYAPVYELAAKYSLPVVIHTGDTYSMNGL-------LKYSHPLTVDELAYQQRGV 176 Y +D++ P+YE+ A+ L + HTG + G+ LKYS+P+ +D++A + Sbjct: 129 YPNDRMAYPLYEVIAEAGLVALFHTGHSGMGTGMPGGGGVRLKYSNPMYIDDVAVDFPEM 188 Query: 177 NFMICHLGDPWVMDAAEVVAKNPNVYADLSGLVVGDRPHFERFMNEPLFMDHFRRALVYC 236 ++ H PW +A V PNVY DLSG ++ E L H+ L+ Sbjct: 189 KIILAHPSFPWQDEALSVCLHKPNVYIDLSGWS-------PKYFPENLV--HYANTLL-- 237 Query: 237 DHYEKMLFGTDWP 249 KMLFG+D+P Sbjct: 238 --KRKMLFGSDYP 248 >ref|NP_949534.1| Amidohydrolase 2 [Rhodopseudomonas palustris CGA009] emb|CAE29639.1| Amidohydrolase 2 [Rhodopseudomonas palustris CGA009] Length = 289 Score = 69.3 bits (168), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 50/179 (27%), Positives = 83/179 (46%), Gaps = 21/179 (11%) Query: 111 VAGIKLYAGYYHYYVHDKIYAPVYELAAKYSLPVVIHTGDTYSMNGL-------LKYSHP 163 + G K + +Y +D++ P+YE + + HTG T +G+ LKYS+P Sbjct: 122 IKGFKFHPTMQGFYANDRLAYPLYEAIQEGGAIALFHTGQTGVGSGMPGGMGMRLKYSNP 181 Query: 164 LTVDELAYQQRGVNFMICHLGDPWVMDAAEVVAKNPNVYADLSGLVVGDRPHFERFMNEP 223 + +D++A + ++ H PW +A V PNVY DLSG P P Sbjct: 182 MYMDDVAADFPDMKIVLAHPSFPWQEEALSVATHKPNVYIDLSGWSPKYFP--------P 233 Query: 224 LFMDHFRRALVYCDHYEKMLFGTDWP-LAPIGLYAEFIRRLVPGQHHEKVFYRNAFELF 281 + + + L +KMLFG+DWP + P ++F + + + KV +NA +L Sbjct: 234 ILVRYINSIL-----QDKMLFGSDWPVITPDRWLSDFAKLEIREEVRAKVLKQNARKLL 287 >ref|NP_343501.1| Conserved hypothetical protein [Sulfolobus solfataricus] pir||S74024 hypothetical protein c0206 - Sulfolobus solfataricus emb|CAA69484.1| orf c02006 [Sulfolobus solfataricus] gb|AAK42291.1| Conserved hypothetical protein [Sulfolobus solfataricus] Length = 269 Score = 68.6 bits (166), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 54/211 (25%), Positives = 99/211 (46%), Gaps = 26/211 (12%) Query: 79 LMECVGINPNRLAGAQAQAELDRIEARMQAPEVAGIKLYAGYYHYYVHDKIYAPVYELAA 138 +++ + ++P++ A EL R ++ E G+KL+ ++ D+ +YE+ Sbjct: 78 IVKFISVDPSK---PNADEELKR---SIKEFEPVGVKLHPQLQGFHPLDERALKIYEIID 131 Query: 139 KYSLPVVIHTGDTYSMNGL-----LKYSHPLTVDELAYQQRGVNFMICHLGDPWVMDAAE 193 + L VV HTG + G+ L Y P+ DE+A + + + ++ H G PW +A Sbjct: 132 NHGLIVVFHTGTSGIGAGVKSSIRLDYGRPIYFDEIAVRFQNMKIILAHFGWPWTEEAIA 191 Query: 194 VVAKNPNVYADLSGLVVGDRPHFERFMNEPLFMDHFRRALVYCDHYEKMLFGTDWPLA-P 252 + PNVY DLSG R++ + ++ + R + +K+LFG+D+PL P Sbjct: 192 IALHKPNVYLDLSGWA-------PRYIPQVIWSNAKRLS-------DKLLFGSDFPLIRP 237 Query: 253 IGLYAEFIRRLVPGQHHEKVFYRNAFELFPR 283 EF + +K+ NA +L + Sbjct: 238 ERWLEEFKSVNLSQDIKDKILKHNAEKLVKK 268 >ref|NP_774189.1| blr7549 [Bradyrhizobium japonicum] dbj|BAC52814.1| blr7549 [Bradyrhizobium japonicum USDA 110] Length = 294 Score = 68.2 bits (165), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 60/213 (28%), Positives = 95/213 (44%), Gaps = 30/213 (14%) Query: 79 LMECVGINPNRLAGAQAQAELDRIEARMQAPE--VAGIKLYAGYYHYYVHDKIYAPVYEL 136 L+ V I+P++ +L EAR E V G K + +Y +D++ P+YE Sbjct: 100 LIPFVSIDPHK-------GKLGAREARKLIEEYGVRGFKFHPTMQGFYANDRMAYPLYEE 152 Query: 137 AAKYSLPVVIHTGDTYSMNGL-------LKYSHPLTVDELAYQQRGVNFMICHLGDPWVM 189 + HTG T +G+ LKYS+P+ +D++A + ++ H PW Sbjct: 153 INNGGAIALFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVAADFPDLKIILAHPSFPWQE 212 Query: 190 DAAEVVAKNPNVYADLSGLVVGDRPHFERFMNEPLFMDHFRRALVYCDHYEKMLFGTDWP 249 +A V PNVY DLSG P P+ + + L +KMLFG+DWP Sbjct: 213 EALSVATHKPNVYIDLSGWSPKYFP--------PILVRYINSIL-----QDKMLFGSDWP 259 Query: 250 -LAPIGLYAEFIRRLVPGQHHEKVFYRNAFELF 281 + P ++F + + + KV NA +L Sbjct: 260 VITPDRWLSDFAKIDIRDEIRPKVLKANARKLL 292 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 435,758,114 Number of extensions: 19255460 Number of successful extensions: 40540 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 40546 Number of HSP's successfully gapped: 23 Length of query: 291 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 167 Effective length of database: 420,565,302 Effective search space: 70234405434 Effective search space used: 70234405434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)