BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0016.p (404 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO46884.1| methionine gamma-lyase [Citrobacter freundii] 387 e-106 ref|NP_783053.1| Cys/Met metabolism lyase (PLP-dependent) [Clost... 385 e-106 dbj|BAC02724.1| L-methionine-alpha-deamino-gamma-mercaptomethane... 377 e-103 ref|NP_617435.1| methionine gamma-lyase [Methanosarcina acetivor... 369 e-101 ref|NP_972801.1| methionine gamma-lyase [Treponema denticola ATC... 366 e-100 >gb|AAO46884.1| methionine gamma-lyase [Citrobacter freundii] Length = 398 Score = 387 bits (994), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/390 (48%), Positives = 255/390 (65%), Gaps = 7/390 (1%) Query: 5 WKMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTS 64 + +T+I+H Q PDP TGA+S + + F Y R GNPT+ Sbjct: 7 YGFNTQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARFALEESGYIYTRLGNPTT 66 Query: 65 RTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPR 124 LEKK+A LE GE L +SG++AI+ LL + GDH++ +YG T+ FL+ P+ Sbjct: 67 DALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYGCTHAFLSHSMPK 126 Query: 125 FGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKLPVIVDN 184 FGI VD + A++P TK VYIETP+NP L+++DI+ + ++VDN Sbjct: 127 FGINVRLVDAGKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDN 186 Query: 185 TFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVI---RFMR-KRLMGDLG 240 TFMSP Q+PL+LGAD+VVHS TKYINGHGDV+ G +VG ++ I RF+ K + G Sbjct: 187 TFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGKQEFIDQARFVGLKDITGGCM 246 Query: 241 QNLNAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLAL 300 NAW L LRG+KT+ +R+ERHC NA +A FLE HPSI RVYYPGL SHPQ++L Sbjct: 247 SPFNAW---LTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLSSHPQYELGQ 303 Query: 301 AQMKGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKER 360 QM GGI+SFE+ GGL G+++INS++L +++ SLGD ETL+QHPASMTH+ + +ER Sbjct: 304 RQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEER 363 Query: 361 VQFGITDGLIRVSVGLEDVDDIIADFEQAL 390 ++ GITDGLIR+SVGLED +DII D E A+ Sbjct: 364 LKAGITDGLIRLSVGLEDPEDIINDLEHAI 393 >ref|NP_783053.1| Cys/Met metabolism lyase (PLP-dependent) [Clostridium tetani E88] gb|AAO36990.1| Cys/Met metabolism lyase (PLP-dependent) [Clostridium tetani E88] Length = 398 Score = 385 bits (990), Expect = e-106, Method: Compositional matrix adjust. Identities = 195/384 (50%), Positives = 257/384 (66%), Gaps = 2/384 (0%) Query: 9 TRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTSRTLE 68 T+ IH D GA+S + + F Y R GNPT+ LE Sbjct: 10 TKAIHGGNKKDTQYGALSTPIYQTSTFIFDSAEQGGKRFAGEESGHIYTRLGNPTTGQLE 69 Query: 69 KKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPRFGIE 128 K+A LEGGE + +SGM AIS AL LK GDHV+ + +YG T+ L PRFG+E Sbjct: 70 DKMAILEGGEAVIATASGMGAISSALWTALKAGDHVVAGETLYGCTFALLCHGLPRFGVE 129 Query: 129 CDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQ-ACKLHKLPVIVDNTFM 187 FVD T+ + +AIK NTK VY+ETP+NP L + DIK I++ A K V VDNTF Sbjct: 130 VTFVDTTNLEEVEKAIKENTKVVYLETPANPNLKVADIKAISEIAHKKEDCMVFVDNTFC 189 Query: 188 SPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGDL-GQNLNAW 246 +PC+Q+PLELGADVVVHSATKY+NGHGDVLAGFVVG ++ I +R + D+ G L+ + Sbjct: 190 TPCIQRPLELGADVVVHSATKYLNGHGDVLAGFVVGKQEFINNVRGFGVKDMTGSVLSPF 249 Query: 247 DSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLALAQMKGM 306 D+FL+LRG+KT+ +R+ERHC NAQ VAEFL+ H ++ +VYYPGL + +A QM+ Sbjct: 250 DAFLVLRGLKTLEIRMERHCANAQKVAEFLQGHEAVSKVYYPGLNDSEYYDIAKKQMRMP 309 Query: 307 GGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKERVQFGIT 366 G ++SFEV GGL EGKK+++S+KLA ++ SLGD ETL+QHPASMTH+ ++ER GI+ Sbjct: 310 GAMISFEVNGGLEEGKKIMDSVKLANLAVSLGDAETLIQHPASMTHSPYTKEEREAAGIS 369 Query: 367 DGLIRVSVGLEDVDDIIADFEQAL 390 D L+R+SVGLE+V+DII D QAL Sbjct: 370 DNLVRLSVGLENVEDIIEDLRQAL 393 >dbj|BAC02724.1| L-methionine-alpha-deamino-gamma-mercaptomethane -lyase [Fusobacterium nucleatum] Length = 395 Score = 377 bits (969), Expect = e-103, Method: Compositional matrix adjust. Identities = 189/341 (55%), Positives = 242/341 (70%), Gaps = 10/341 (2%) Query: 56 YGRYGNPTSRTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTY 115 Y R GNPT+ LE KIAALE GE A+ SSGM AIS L LK GDHV+ K +YG T+ Sbjct: 56 YTRLGNPTTTVLENKIAALEEGEAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTF 115 Query: 116 NFLTSLAPRFGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKL 175 + RFGIE FVD ++ + A+K NT+ VY+ETP+NP L I+D++ +++ Sbjct: 116 ALMCHGLTRFGIEVTFVDTSNLDEVKNAMKKNTRVVYLETPANPNLKIVDLEALSKLA-- 173 Query: 176 HKLP---VIVDNTFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSK---DVIR 229 H P VIVDNTF +P +QKPL+LGAD+VVHS TKYINGHGDV+AG V+ +K D IR Sbjct: 174 HTNPNTLVIVDNTFATPYMQKPLKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIR 233 Query: 230 FMRKRLMGDLGQNLNAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPG 289 F+ + M G L D++ I+RGMKT +R+ERHC+NA+ V EFL HP IERVYYPG Sbjct: 234 FIGLKDM--TGAVLGPQDAYYIIRGMKTFEIRMERHCKNAKKVVEFLNKHPKIERVYYPG 291 Query: 290 LESHPQHKLALAQMKGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPAS 349 LE+HP H++A QMK G ++SFE+KGG GK L+N+LKL ++ SLGD ETL+QHPAS Sbjct: 292 LETHPGHEIAKKQMKDFGAMISFELKGGFEAGKTLLNNLKLCSLAVSLGDTETLIQHPAS 351 Query: 350 MTHASIPQKERVQFGITDGLIRVSVGLEDVDDIIADFEQAL 390 MTH+ ++ER GITDGL+R+SVGLE+V+DIIAD EQ L Sbjct: 352 MTHSPYTKEEREAAGITDGLVRLSVGLENVEDIIADLEQGL 392 >ref|NP_617435.1| methionine gamma-lyase [Methanosarcina acetivorans str. C2A] gb|AAM05915.1| methionine gamma-lyase [Methanosarcina acetivorans str. C2A] Length = 406 Score = 369 bits (947), Expect = e-101, Method: Compositional matrix adjust. Identities = 180/396 (45%), Positives = 259/396 (65%), Gaps = 3/396 (0%) Query: 1 MQAEWKMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRY- 59 M+ E K T+ +H + PDP+ GA + + + F + Y R Sbjct: 1 MERELKFATKCVHAGEEPDPVFGAHTTPIFQTSTFIFKNVEQGAARFAGEESGYVYTRIP 60 Query: 60 -GNPTSRTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFL 118 PT L +K+A LEGGE +SGMAAI+ +L LK GDH++ T VYG TY+ Sbjct: 61 PNTPTHAVLAEKVAVLEGGEAGQTFASGMAAITAVVLSTLKQGDHLISTDVVYGCTYSLF 120 Query: 119 TSLAPRFGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKL 178 + + P FGI+ FVD +D + A KP T+ V++ETP+NP +T+ DI++I++ K Sbjct: 121 SEILPGFGIDVSFVDTSDIKKVRAAFKPETRMVFLETPANPTITVCDIREISKLAKAQGA 180 Query: 179 PVIVDNTFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGD 238 IVDNTF +P QKPLELGAD+ + S TKYI GH D+L G VVGS+D ++ + K ++G Sbjct: 181 ICIVDNTFATPYFQKPLELGADISLSSCTKYIGGHADLLGGIVVGSRDFMKSLGK-VVGY 239 Query: 239 LGQNLNAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKL 298 G + +++L +RG+KT+ +R+ERH NA TVA FLEAHP++E V YPGL +HPQH++ Sbjct: 240 TGGIMGLHEAWLCIRGLKTLHIRMERHAENALTVANFLEAHPTVEWVRYPGLSNHPQHEV 299 Query: 299 ALAQMKGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQK 358 A QM G GG++SFE++GG+ G++L++S++L ++ SLG +TL+QHPASMTHA IP Sbjct: 300 AKKQMSGYGGMLSFEIRGGVEAGRRLMDSVRLCSLAVSLGATDTLIQHPASMTHACIPGH 359 Query: 359 ERVQFGITDGLIRVSVGLEDVDDIIADFEQALAALF 394 R + GI DGL+R+SVG+ED +DIIAD EQAL+ ++ Sbjct: 360 IRNKVGIKDGLVRLSVGIEDPEDIIADLEQALSKVY 395 >ref|NP_972801.1| methionine gamma-lyase [Treponema denticola ATCC 35405] gb|AAS12720.1| methionine gamma-lyase [Treponema denticola ATCC 35405] Length = 401 Score = 366 bits (940), Expect = e-100, Method: Compositional matrix adjust. Identities = 181/374 (48%), Positives = 251/374 (67%), Gaps = 3/374 (0%) Query: 23 GAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTSRTLEKKIAALEGGEDALG 82 GA++ + +AF Y R GNPT+ +E+K+A LE GE + Sbjct: 26 GALATPIYQTSTFAFDSAEQGGRRFALEEEGYIYTRLGNPTTTVVEEKLACLENGEACMS 85 Query: 83 VSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPRFGIECDFVDCTDTAAMLQ 142 SSG+ A++ + + GDH++ K +YG T+ FL RFG++ FVD D + + Sbjct: 86 ASSGIGAVTSCIWSIVNAGDHIVAGKTLYGCTFAFLNHGLSRFGVDVTFVDTRDPENVKK 145 Query: 143 AIKPNTKAVYIETPSNPKLTILDIKQITQACKLH--KLPVIVDNTFMSPCLQKPLELGAD 200 A+KPNTK VY+ETP+NP + + DI +++ H + VIVDNT+M+P LQ+PL+LGAD Sbjct: 146 ALKPNTKIVYLETPANPNMYLCDIAAVSKIAHAHNPECKVIVDNTYMTPYLQRPLDLGAD 205 Query: 201 VVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGDL-GQNLNAWDSFLILRGMKTMA 259 VV+HSATKY+NGHGDV+AGFVVG K+ I +R + D+ G L ++++LI RGMKT+ Sbjct: 206 VVLHSATKYLNGHGDVIAGFVVGKKEFIDQVRFVGVKDMTGSTLGPFEAYLIGRGMKTLD 265 Query: 260 LRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLALAQMKGMGGIVSFEVKGGLT 319 +R+E+HC NAQ VAEFLE HP++E + +PGL+S PQ++LA QMK G +++F VKGGL Sbjct: 266 IRMEKHCANAQKVAEFLEKHPAVESIAFPGLKSFPQYELAKKQMKLCGAMIAFTVKGGLE 325 Query: 320 EGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKERVQFGITDGLIRVSVGLEDV 379 GK LINS+K A I+ SLGD ETL+QHPASMTH+ +ER I +GL+R+SVGLED Sbjct: 326 AGKTLINSVKFATIAVSLGDAETLIQHPASMTHSPYTPEERAASDIAEGLVRLSVGLEDA 385 Query: 380 DDIIADFEQALAAL 393 +DIIAD +QAL L Sbjct: 386 EDIIADLKQALDKL 399 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 566,909,732 Number of extensions: 22064101 Number of successful extensions: 58290 Number of sequences better than 1.0e-05: 1955 Number of HSP's gapped: 55375 Number of HSP's successfully gapped: 2288 Length of query: 404 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 276 Effective length of database: 409,704,906 Effective search space: 113078554056 Effective search space used: 113078554056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)