BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0009.p (586 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAA22770.1| subtilin >gi|143566|gb|AAA22776.1| this protein h... 254 3e-66 gb|AAL15565.1| putative SpaT [Bacillus subtilis] 254 6e-66 sp|P33116|SPAT_BACSU Subtilin transport ATP-binding protein spaT... 253 8e-66 ref|NP_488995.1| ATP-binding protein of ABC transporter [Nostoc ... 251 3e-65 ref|ZP_00108235.1| COG1132: ABC-type multidrug transport system,... 240 9e-62 >gb|AAA22770.1| subtilin gb|AAA22776.1| this protein homologous to HlyB, which participates in the secretion of hemolysin protein in E.coli Length = 599 Score = 254 bits (650), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 152/528 (28%), Positives = 275/528 (52%), Gaps = 10/528 (1%) Query: 65 LVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQVNKKLSVISMEQLENPAVYD 124 ++ I Y + E I+ N + + NI + +V KK S ++++ ENP +YD Sbjct: 57 VITIFLTYLGVSFFSELISQISEFYNGKFQLNIGYKLNYKVMKKSSNLALKDFENPEIYD 116 Query: 125 LLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXXXXXXXAVLATVPYLLLMN 184 L+RV ++ I +++ ++ V L Sbjct: 117 KLERVTKEISYKPYQIIQAIITMTTSFVTLLSSIAFLMSWNPKVSLLLLVIPVISLFYFL 176 Query: 185 AQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLIDYFNDRTEEIRKDVWKEK 244 G++ +F +++ R+ Y+ +LT KE++++ L DY ++ +I+K ++ Sbjct: 177 KIGQEEFFIHWKRAGKERKSWYISYILTHDFSFKELKLYNLKDYLLNKYWDIKKSFIEQD 236 Query: 245 FKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGM 304 K+L TL +I + + + + + ++ +G+VM I S+ + + + Sbjct: 237 TKILRKKTLLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNVMSYIRSVSLVQNHSQSI 296 Query: 305 VNKISSMNNYTLYIEDMMNFLELPEDTGSGHKKIVESP--TIQFKHVDFKYPNSKTHTLK 362 + I S+ N LY+ + FLEL E+ GHKK + P ++ F++V F YPN TLK Sbjct: 297 MTSIYSIYNSNLYMNQLYEFLELKEEKSQGHKKPIVEPIHSVVFQNVSFIYPNQGEQTLK 356 Query: 363 NIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTV 421 +I+++++KGE++A+VG NG GK+TFI +L GLY+ G++L+ G + ++ +D + Sbjct: 357 HINVSLHKGERVAIVGPNGSGKSTFIKLLTGLYEVQQGDILINGINIKELDMDSYMNQIA 416 Query: 422 CVFQNFIKYQLSVADNIKAGNFGEDFHK-----QSLQIFSMDSFIDNLPKGLDTQLGQLE 476 +FQ+F+KY++++ +NI G + H+ + L I D + DTQLG Sbjct: 417 ALFQDFMKYEMTLKENIGFGQI-DKLHQTNKMHEVLDIVRADFLKSHSSYQFDTQLGLWF 475 Query: 477 NNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIM 536 + +LSGGQWQ++A+ARA R + + I+DEP+++LDP E + ++ F +L ++K I Sbjct: 476 DEGRQLSGGQWQKIALARAYFREAS-LYILDEPSSALDPIAEKETFDTFFSLSKDKIGIF 534 Query: 537 ISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYSAQSYL 584 ISHRL +L D I V D+GEI G+H EL+ Y +M +++Y+ Sbjct: 535 ISHRLVAAKLADRIIVMDKGEIVGIGTHEELLKTCPLYKKMDESENYM 582 >gb|AAL15565.1| putative SpaT [Bacillus subtilis] Length = 614 Score = 254 bits (648), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 153/528 (28%), Positives = 274/528 (51%), Gaps = 10/528 (1%) Query: 65 LVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQVNKKLSVISMEQLENPAVYD 124 ++ I Y + E I+ + N + + NI + +V KK S ++++ ENP +YD Sbjct: 72 VISIFLTYLGVSFFSELISQISEYYNGKFQLNIGYKLNYKVMKKSSNLALKDFENPEIYD 131 Query: 125 LLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXXXXXXXAVLATVPYLLLMN 184 L+RV ++ I +++ ++ V L Sbjct: 132 KLERVTKEISYKPYQIIQAIITMTTSCVTLLSSIAFLMSWNPKVSLLLLVIPVISLFYFL 191 Query: 185 AQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLIDYFNDRTEEIRKDVWKEK 244 G++ +F +++ R+ Y+ +LT KE++++ L DY ++ +I+K + Sbjct: 192 KIGQQEFFIHWKRAGQERKSWYISYILTHDFSFKELKLYNLKDYLLNKYWDIKKTFIGQD 251 Query: 245 FKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGM 304 K+L T +I + + + + + ++ +G+VM I S+ + + + Sbjct: 252 TKILRKKTYLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNVMSYIRSVSLVQNHSQSI 311 Query: 305 VNKISSMNNYTLYIEDMMNFLELPEDTGSGHKKIVESP--TIQFKHVDFKYPNSKTHTLK 362 + I S+ N LY+ + FLEL E+ GHKK + P ++ F++V F YPN TLK Sbjct: 312 MTSIYSIYNSNLYMNQLYEFLELKEEKNEGHKKQIVEPIDSVVFQNVSFIYPNQGEQTLK 371 Query: 363 NIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTV 421 +I+++++KGE++A+VG NG GK+TFI +L GLY+ G++L+ G + ++ +D + Sbjct: 372 HINVSLHKGERVAIVGPNGSGKSTFIKLLTGLYEVQQGDILINGINIKELDMDSYMNQIA 431 Query: 422 CVFQNFIKYQLSVADNIKAGNFGEDFHK-----QSLQIFSMDSFIDNLPKGLDTQLGQLE 476 +FQ+F+KY++++ +NI G + H+ + L I +D + DTQLG Sbjct: 432 ALFQDFMKYEMTLKENIGFGQI-DKLHQTNKMHEVLDIVKVDFLKSHSSYQFDTQLGLWF 490 Query: 477 NNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIM 536 + +LSGGQWQ++A+ARA R + + I+DEP+A+LDP E +I++ F +L ++K I Sbjct: 491 DEGRQLSGGQWQKIALARAYFREAS-MYILDEPSAALDPIAEKEIFDTFFSLSKDKIGIF 549 Query: 537 ISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYSAQSYL 584 ISHRL +L D I V D GEI G H EL+ Y +M +++Y+ Sbjct: 550 ISHRLVAAKLADRIIVMDNGEIVGIGKHEELLKTCPLYKKMDESENYM 597 >sp|P33116|SPAT_BACSU Subtilin transport ATP-binding protein spaT pir||B43935 spaT protein - Bacillus subtilis gb|AAA22838.1| involved in the biosynthesis of subtilin gb|AAB91587.1| SpaT [Bacillus subtilis] Length = 614 Score = 253 bits (647), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 155/545 (28%), Positives = 281/545 (51%), Gaps = 13/545 (2%) Query: 48 LIDDLAFVHSASAVTQKLVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQVNK 107 LI+ L + ++ ++ I Y + E I+ N + + NI + +V K Sbjct: 58 LINSLVTIRKEVSI---VITIFLTYLGVSFFSELISQISEFYNGKFQLNIGYKLNYKVMK 114 Query: 108 KLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXXX 167 K S ++++ ENP +YD L+RV ++ I +++ Sbjct: 115 KSSNLALKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSFVTLLSSIAFLMSWNPK 174 Query: 168 XXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLID 227 ++ V L G++ +F +++ R+ Y+ +LT KE++++ L D Sbjct: 175 VSLLLLVIPVISLFYFLKIGQEEFFIHWKRAGKERKSWYISYILTHDFSFKELKLYNLKD 234 Query: 228 YFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDV 287 Y ++ +I+K ++ K+L TL +I + + + + + ++ +G+V Sbjct: 235 YLLNKYWDIKKSFIEQDTKILRKKTLLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNV 294 Query: 288 MLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLELPEDTGSGHKKIVESP--TIQ 345 M I S+ + + ++ I S+ N LY+ + FLEL E+ GHKK + P ++ Sbjct: 295 MSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQGHKKPIVEPIHSVV 354 Query: 346 FKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVG 405 F++V F YPN TLK+I+++++KGE++A+VG NG GK TFI +L GLY+ G++L+ Sbjct: 355 FQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKKTFIKLLTGLYEVHEGDILIN 414 Query: 406 GDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHK-----QSLQIFSMDS 459 G + ++ +D + +FQ+F+KY++++ +NI G + H+ + L I D Sbjct: 415 GINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQI-DKLHQTNKMHEVLDIVRADF 473 Query: 460 FIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVET 519 + DTQLG + +LSGGQWQ++A+ARA R + + I+DEP+++LDP E Sbjct: 474 LKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREAS-LYILDEPSSALDPIAEK 532 Query: 520 QIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYS 579 + ++ F +L ++K I ISHRL +L D I V D+GEI G+H EL+ Y +M Sbjct: 533 ETFDTFFSLSKDKIGIFISHRLVAAKLADRIIVMDKGEIVGIGTHEELLKTCPLYKKMDE 592 Query: 580 AQSYL 584 +++Y+ Sbjct: 593 SENYM 597 >ref|NP_488995.1| ATP-binding protein of ABC transporter [Nostoc sp. PCC 7120] pir||AC2425 ATP-binding protein of ABC transporter all4955 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB76654.1| ATP-binding protein of ABC transporter [Nostoc sp. PCC 7120] Length = 606 Score = 251 bits (641), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 146/486 (30%), Positives = 241/486 (49%), Gaps = 7/486 (1%) Query: 107 KKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXX 166 KK + + EN YD L + + LS +N + +V Sbjct: 120 KKALTLDLTHFENSEFYDKLTNARREASVRPLSLVNRTFGLVQNALSLITFGFLLVKFSI 179 Query: 167 XXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLI 226 ++A +P + +++ ++ R+ +Y+ ++L +A E++++ L Sbjct: 180 WAVIVLIVAAMPAFIAETRFAGEAFRLFSWRAPETRQQHYIENLLAREDFATEVKLYQLG 239 Query: 227 DYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGD 286 D R + + ++ E L + + G + + VA I I + R+S+GD Sbjct: 240 DMLLGRYRNVFRQLYNEDRDLTIRRGMWGYLLGLVSTVAFYIAYAWIVIEAVVGRISLGD 299 Query: 287 VMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLE--LPEDTGSGHKKIVESPTI 344 + + +T + + + I M LY+ ++ +FLE + + G K I I Sbjct: 300 MTMYLTVFRQGQSTFANALTSIGGMYEDNLYLSNLYDFLEEKVTQPWGKITKGIDTQDGI 359 Query: 345 QFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLV 404 +F++V F YP S LKNI + + GEK+A+VGENG GKTT I +L LY P SG +L+ Sbjct: 360 RFENVTFTYPGSSQPALKNISLHLKPGEKLAIVGENGSGKTTLIKLLTRLYTPDSGRILL 419 Query: 405 GGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHKQSLQIFSMD----S 459 G L +D R +FQNF++YQ +V +NI G+ QI + S Sbjct: 420 DGVDLQSWNVDALRQRIGVIFQNFVRYQFTVGENIGVGDVDSLNDTTRWQIAAQKGNAHS 479 Query: 460 FIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVET 519 FI+ LP+ TQLG+ + ELSGGQWQ++A+ARA R +IL++DEPT+++D + E Sbjct: 480 FIERLPQQFQTQLGKWFKSGQELSGGQWQKIALARAFMRTQADILVLDEPTSAIDAQAEY 539 Query: 520 QIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYS 579 +I+ F AL +N+ +ISHR R+ D I V + GE+ E G+H EL+ R G Y +++ Sbjct: 540 EIFNHFRALTQNQMVFLISHRFSTVRMADKILVIENGEVREQGTHEELLAREGLYAKLFL 599 Query: 580 AQSYLY 585 Q+ Y Sbjct: 600 LQAAGY 605 >ref|ZP_00108235.1| COG1132: ABC-type multidrug transport system, ATPase and permease components [Nostoc punctiforme] Length = 587 Score = 240 bits (612), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 136/486 (27%), Positives = 238/486 (48%), Gaps = 7/486 (1%) Query: 107 KKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXX 166 +K + + Q E+ YD L + + LS +N + +V Sbjct: 101 EKALTLDLRQFEDSEFYDKLTNARREASVRPLSLVNRTFGLVQNALSLITYGILLVNFSV 160 Query: 167 XXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLI 226 +LA +P + + + ++ R+ +Y+ ++L + E++++ L Sbjct: 161 WAVVVLILAAMPVFIAETKFAGEGFRLFSWRAAETRQQHYLENLLAREDFVTEVKLYQLG 220 Query: 227 DYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGD 286 + R + ++ E L L G + + A + + + ++S+GD Sbjct: 221 EMLLGRYRNLFHQLYGEDRDLTLRRGFWGYLLGLVSTSAFYLAYAWIVLETVRGKISLGD 280 Query: 287 VMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLE--LPEDTGSGHKKIVESPTI 344 + + +T + S + I M LY+ ++ +FLE +P+ G + I Sbjct: 281 MTMYLTVFRQGQSTFSNALTSIGGMYEDNLYLSNLYDFLEEEVPKSWGKATIGLNPQDGI 340 Query: 345 QFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLV 404 +F++V F YP S L NI + + EK+A+VGENG GKTT I +L LY P SG + + Sbjct: 341 RFENVSFTYPGSSKPALTNISLHLKPREKLAIVGENGSGKTTLIKLLTRLYTPDSGRIFL 400 Query: 405 GGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHKQSLQIFS----MDS 459 G L + +D R +FQNF++YQ SV +NI G+ +K + + S Sbjct: 401 DGLDLQEWDVDVLRRRIGVIFQNFVRYQFSVGENIGVGDVEHLENKNRWETAAEKGMAQS 460 Query: 460 FIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVET 519 FID LP+ TQLG+ ELSGGQWQ++A++RA R+ +IL++DEPT+++D + E Sbjct: 461 FIDQLPQSFQTQLGRWFKGGQELSGGQWQKIALSRAFMRSQADILVLDEPTSAIDAQAEF 520 Query: 520 QIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYS 579 +I+ F A+ +N+ ++ISHR R+ D I V + GE+ E G+H EL+ +G+Y +++ Sbjct: 521 EIFNHFRAITQNQMVLLISHRFSTVRMADKIVVIENGEVIEQGTHEELLQLKGRYAKLFL 580 Query: 580 AQSYLY 585 Q+ Y Sbjct: 581 LQAAGY 586 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 789,532,444 Number of extensions: 31791250 Number of successful extensions: 164908 Number of sequences better than 1.0e-05: 15942 Number of HSP's gapped: 143034 Number of HSP's successfully gapped: 16969 Length of query: 586 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 455 Effective length of database: 401,559,609 Effective search space: 182709622095 Effective search space used: 182709622095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)