BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0003.p (498 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAL82575.1| exo-inulinase [Paenibacillus polymyxa] 627 e-178 gb|AAF44125.1| exo-inulinase [Pseudomonas mucidolens] 550 e-155 dbj|BAC45010.1| exo-inulinase [Geobacillus stearothermophilus] 481 e-134 ref|NP_390581.1| levanase [Bacillus subtilis] >gi|134175|sp|P056... 478 e-133 pir||A27286 levanase (EC 3.2.1.65) precursor - Bacillus subtilis... 478 e-133 >gb|AAL82575.1| exo-inulinase [Paenibacillus polymyxa] Length = 485 Score = 627 bits (1618), Expect = e-178, Method: Compositional matrix adjust. Identities = 294/484 (60%), Positives = 357/484 (73%) Query: 1 MPASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKD 60 M ++ YR YHFSP++ WMNDPNGMVY+EG YHLF+QHHP G T G MHWGHAVS D Sbjct: 1 MNVIRQEKYRPSYHFSPKSGWMNDPNGMVYYEGRYHLFYQHHPFGTTWGPMHWGHAVSTD 60 Query: 61 LVTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 L+ WEE P+AL PDELG IFSGSAVVD +T+GFF KPGLVAIFTHH +P + Q Sbjct: 61 LMNWEEQPIALEPDELGTIFSGSAVVDEQDTSGFFGGKPGLVAIFTHHASLPGTEQVRQC 120 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 QSLAYS D+G+TW KY GNPVLED IDFRDPKVFW++ T +WVM++ACGQT+ +YHSP Sbjct: 121 QSLAYSTDSGKTWIKYTGNPVLEDKECIDFRDPKVFWHKPTEQWVMVLACGQTIRIYHSP 180 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 +L WTFASEFG GIGSHD VWECPDLFPL++D +KWVMLVSIG P EGSRTQ Sbjct: 181 NLKEWTFASEFGHGIGSHDAVWECPDLFPLYIDEKREQVKWVMLVSIGDVPEIREGSRTQ 240 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQT 300 YFTG+FDG F D +S +RWLDYGRDNYAGV WSD+P DGRRLF+GWMSNW YA QT Sbjct: 241 YFTGEFDGTTFVADAESEKVRWLDYGRDNYAGVCWSDIPAEDGRRLFMGWMSNWRYANQT 300 Query: 301 PAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASLAGLQ 360 P E +RGAM++PREL LE+R+G + LVQ+P +ELE R PVLSL + + +EV +L+ LQ Sbjct: 301 PTERWRGAMSIPRELALETRKGTVALVQRPVRELEGLRTPVLSLTEPSWEEVRNALSALQ 360 Query: 361 LDAYEIAAKLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERYPGRHS 420 LD YE+ A+ GFKVRV +ET+ G + T E+YVDR SG + FHE + G+H Sbjct: 361 LDCYELVAEFATTGDFGFKVRVSDEQETLVGYTSATQEVYVDRKRSGRSDFHEDFAGKHG 420 Query: 421 AALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSLE 480 A+L + D+ IYVDR+S+EVF + GQ ITDLIFP E+ G+ F+ E+ SLE Sbjct: 421 ASLTILPDRMDIRIYVDRSSIEVFFDRGQVAITDLIFPDAEAKGLEIFSAEEQITVFSLE 480 Query: 481 VYKL 484 +Y L Sbjct: 481 LYAL 484 >gb|AAF44125.1| exo-inulinase [Pseudomonas mucidolens] Length = 501 Score = 550 bits (1417), Expect = e-155, Method: Compositional matrix adjust. Identities = 260/481 (54%), Positives = 328/481 (68%), Gaps = 2/481 (0%) Query: 6 KQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWE 65 ++ +R +HF+P+ WMNDPNG+VY+EGEYHLF+Q+HP G T G MHWGHAVSKDL+ W+ Sbjct: 7 QEKFRPQFHFTPETNWMNDPNGLVYYEGEYHLFYQYHPFGNTWGPMHWGHAVSKDLIHWD 66 Query: 66 ELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQRQSLAY 125 LP+AL+PD +G IFSGSAV+DW +T+GFF G+VA+FTH P+ P QRQSLAY Sbjct: 67 HLPIALYPDHIGQIFSGSAVIDWQDTSGFFNGGAGMVALFTHADKFPDSGRPRQRQSLAY 126 Query: 126 SHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSPDLIHW 185 S D GRTW KYEGNP D+A DFRDPKVFW++ + KW+MI+A G V +YHS +L W Sbjct: 127 STDRGRTWIKYEGNPFFADEAMTDFRDPKVFWHKASNKWIMILAAGNCVRIYHSSNLKQW 186 Query: 186 TFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQYFTGD 245 FASEFG GSH GVWECPDLF L VDGD + KWVM+VSIG + EGSRTQYF G Sbjct: 187 KFASEFGANEGSHTGVWECPDLFELPVDGDRNRKKWVMVVSIGNSDEYLEGSRTQYFIGQ 246 Query: 246 FDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQTPAEEF 305 FDG FT + D T+ W+D+GRDNYAGV+WSD+PE DGRRLFI WMSNW YA TP E + Sbjct: 247 FDGIRFTNENDPETVLWVDHGRDNYAGVTWSDIPEEDGRRLFIAWMSNWKYANHTPTEIW 306 Query: 306 RGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASLAGLQLDAYE 365 R AMT+PR L L S L Q P ELE+ R+ L KD+ V L+ + D +E Sbjct: 307 RSAMTIPRSLSLRSTPEGTRLFQTPVTELESLRLEKLEWKDLEVTTNGNVLSNISGDIFE 366 Query: 366 IAA--KLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERYPGRHSAAL 423 I A +L + GFKVR A +ET+ G DA+ L++DR SG++ F E +P +H A + Sbjct: 367 IIAEFELNTALEFGFKVRKSADQETIVGYDAEQQMLFIDRTQSGVSDFCEHFPCKHGAVM 426 Query: 424 QAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSLEVYK 483 +HI+VDR+SVEVFGN G+ +TDLIFP S GI +A+ +SL ++ Sbjct: 427 IPNQNRIQMHIFVDRSSVEVFGNNGELTMTDLIFPDDSSTGIEVYAKEGNVKLVSLMLFP 486 Query: 484 L 484 L Sbjct: 487 L 487 >dbj|BAC45010.1| exo-inulinase [Geobacillus stearothermophilus] Length = 493 Score = 481 bits (1237), Expect = e-134, Method: Compositional matrix adjust. Identities = 231/476 (48%), Positives = 305/476 (64%), Gaps = 14/476 (2%) Query: 1 MPASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKD 60 M + YR +HFSP+ WMNDPNG+VYF+GEYHLFFQH+P+ G MHWGHAVSKD Sbjct: 1 MKTHNSEKYRPTFHFSPKKNWMNDPNGLVYFKGEYHLFFQHNPNDSIWGPMHWGHAVSKD 60 Query: 61 LVTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 ++ WEEL +AL+PDE G IFSGS V+DWNNT+GFF +PG+VAIFT +L + P+Q Sbjct: 61 MIEWEELDIALYPDENGTIFSGSVVIDWNNTSGFFPKEPGMVAIFTQNLHDADHTTPIQT 120 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 QSLA+SHD GRTWTKYE NPVL+ +DFRDPKVFW+ E+ KW+M++A GQTV +Y SP Sbjct: 121 QSLAFSHDRGRTWTKYEKNPVLKHPTKVDFRDPKVFWHYESEKWIMVLATGQTVSIYSSP 180 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 +LI W F SEFG+ IG HDGVWECPDLF L V+ ++ KWVM VSIG + F GSRTQ Sbjct: 181 NLIDWQFESEFGENIGCHDGVWECPDLFELPVE-NSEEKKWVMFVSIGDNRQFDSGSRTQ 239 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQT 300 YF G FDG+ F DE++ + WLD+G+DNYAGV++SD+P+ DGRR++IGWMSNW YA + Sbjct: 240 YFIGSFDGSNFKVDENNNEVLWLDFGKDNYAGVTFSDIPKEDGRRIYIGWMSNWRYANEV 299 Query: 301 PAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQ-------EVN 353 P +RG MTLPR L L + ++Q P +EL+ + + D + ++N Sbjct: 300 PTVGWRGQMTLPRVLSLRKIGDKLRVIQNPVKELDNYFTKQIVIHDEIISSGIQKKIDIN 359 Query: 354 ASLAGLQLDAYEIAAKLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHE 413 + L+ + AK G + + T ID T L +DR SG F E Sbjct: 360 QPSVEIVLNVNNLGAK-----QFGLVLHHTENQSTTITIDLLTNLLSLDRTNSGEVSFSE 414 Query: 414 RYPGRHSAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFA 469 + + ++ +L I VD +SVE+F N G +T LI+P I+ F+ Sbjct: 415 NFLNNQVMKIHDTTKL-NLRIIVDSSSVEIFVNEGAHALTSLIYPDKACEKISIFS 469 >ref|NP_390581.1| levanase [Bacillus subtilis] sp|P05656|SACC_BACSU Levanase precursor (2,6-beta-D-fructan fructanohydrolase) emb|CAA63465.1| levanase precursor [Bacillus subtilis] emb|CAB14645.1| levanase [Bacillus subtilis subsp. subtilis str. 168] Length = 677 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 251/485 (51%), Positives = 318/485 (65%), Gaps = 14/485 (2%) Query: 7 QNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWEE 66 ++YR YHF+P+A WMNDPNGMVY+ GEYHLF+Q+HP G+ G MHWGHAVSKDLVTWE Sbjct: 32 EDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVSKDLVTWEH 91 Query: 67 LPVALFPDELGMIFSGSAVVDWNNTTGFFADKPG-LVAIFTHHLDMPEGQPPVQRQSLAY 125 LPVAL+PDE G IFSGSAVVD NNT+GF K LVAI+T EG Q QS+AY Sbjct: 92 LPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDR---EGH---QVQSIAY 145 Query: 126 SHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSPDLIHW 185 S+D GRTWTKY GNPV+ + DFRDPKVFW + KWVM++A G + +Y S +L W Sbjct: 146 SNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQW 205 Query: 186 TFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQYFTGD 245 T+ASEFG+ GSH GVWECPDLF L VDG+ + KWVM VS+G + A + GS QYF GD Sbjct: 206 TYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGGSGMQYFVGD 264 Query: 246 FDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQTPAEEF 305 FDG F + + W DYGRD YA VSWSD+P D RRL++GWMSNW YA P + Sbjct: 265 FDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVPTSPW 324 Query: 306 RGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNAS-LAGLQLDAY 364 R A ++PREL L++ + +VQ P +ELE R K++T+ + + LAG DAY Sbjct: 325 RSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAY 384 Query: 365 EIAA--KLPRGMSA--GFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERY-PGRH 419 EI A K+ G +A GFKVR G + T G D + +L+VDR+ SG F+ + G+ Sbjct: 385 EINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKE 444 Query: 420 SAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSL 479 +A L+ L I+VDR+SVEVFGN G+ VITD+I P S G+ +A + SL Sbjct: 445 TAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAANGGVKVKSL 504 Query: 480 EVYKL 484 ++ L Sbjct: 505 TIHPL 509 >pir||A27286 levanase (EC 3.2.1.65) precursor - Bacillus subtilis emb|CAA68542.1| unnamed protein product [Bacillus subtilis] Length = 677 Score = 478 bits (1229), Expect = e-133, Method: Compositional matrix adjust. Identities = 251/485 (51%), Positives = 318/485 (65%), Gaps = 14/485 (2%) Query: 7 QNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWEE 66 ++YR YHF+P+A WMNDPNGMVY+ GEYHLF+Q+HP G+ G MHWGHAVSKDLVTWE Sbjct: 32 EDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVSKDLVTWEH 91 Query: 67 LPVALFPDELGMIFSGSAVVDWNNTTGFFADKPG-LVAIFTHHLDMPEGQPPVQRQSLAY 125 LPVAL+PDE G IFSGSAVVD NNT+GF K LVAI+T EG Q QS+AY Sbjct: 92 LPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDR---EGH---QVQSIAY 145 Query: 126 SHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSPDLIHW 185 S+D GRTWTKY GNPV+ + DFRDPKVFW + KWVM++A G + +Y S +L W Sbjct: 146 SNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQW 205 Query: 186 TFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQYFTGD 245 T+ASEFG+ GSH GVWECPDLF L VDG+ + KWVM VS+G + A + GS QYF GD Sbjct: 206 TYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVG-NGAVSGGSGMQYFVGD 264 Query: 246 FDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQTPAEEF 305 FDG F + + W DYGRD YA VSWSD+P D RRL++GWMSNW YA P + Sbjct: 265 FDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVPTSPW 324 Query: 306 RGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNAS-LAGLQLDAY 364 R A ++PREL L++ + +VQ P +ELE R K++T+ + + LAG DAY Sbjct: 325 RSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAY 384 Query: 365 EIAA--KLPRGMSA--GFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERY-PGRH 419 EI A K+ G +A GFKVR G + T G D + +L+VDR+ SG F+ + G+ Sbjct: 385 EINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKE 444 Query: 420 SAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSL 479 +A L+ L I+VDR+SVEVFGN G+ VITD+I P S G+ +A + SL Sbjct: 445 TAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAANGGVKVKSL 504 Query: 480 EVYKL 484 ++ L Sbjct: 505 TIHPL 509 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 848,490,470 Number of extensions: 39532418 Number of successful extensions: 71582 Number of sequences better than 1.0e-05: 388 Number of HSP's gapped: 70351 Number of HSP's successfully gapped: 408 Length of query: 498 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 368 Effective length of database: 404,274,708 Effective search space: 148773092544 Effective search space used: 148773092544 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)