BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0530.0021.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_462502.1| putative phosphosugar isomerase [Salmonella typ... 264 2e-69 ref|NP_350087.1| Predicted phosphosugar isomerase [Clostridium a... 260 4e-68 ref|NP_391141.1| similar to opine catabolism [Bacillus subtilis]... 244 2e-63 ref|NP_459564.1| putative phosphosugar isomerases [Salmonella ty... 241 1e-62 ref|ZP_00036355.1| COG2222: Predicted phosphosugar isomerases [E... 231 2e-59 >ref|NP_462502.1| putative phosphosugar isomerase [Salmonella typhimurium LT2] gb|AAL22461.1| putative phosphosugar isomerase [Salmonella typhimurium LT2] Length = 325 Score = 264 bits (675), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 189/324 (58%), Gaps = 1/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M +K+ +S+I + E GG+KH+Y+VACGGS AA + ++KEA+ +YNS EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGVKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 + P L E +VV+ S T ET++A ++A + GA I +T + + + Y Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETY 121 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 + G+ + + L + E+L Q E YAHY + +K++ IV A + A Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA EYKDD++ Y +ASG Y AY + C FMEMQW H+ +HSGE+FHGPFE TD N P Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 SEG TR +DERAL FL +Y +R+ V+DAKELG++ I +V +YF + V Sbjct: 242 FFFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +AE R H + RRYMWKVEY Sbjct: 302 YNRALAEARQHPLTTRRYMWKVEY 325 >ref|NP_350087.1| Predicted phosphosugar isomerase [Clostridium acetobutylicum] pir||H97329 probable phosphosugar isomerase [imported] - Clostridium acetobutylicum gb|AAK81427.1| Predicted phosphosugar isomerase [Clostridium acetobutylicum] Length = 336 Score = 260 bits (664), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 135/330 (40%), Positives = 191/330 (57%), Gaps = 8/330 (2%) Query: 2 EIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVH 61 +IK IIS+I K K GG+K IY V CGGS A++ ++KE + ++ NSSEF+H Sbjct: 7 DIKSIISEILDKKKAKGGVKSIYVVGCGGSLGALYPAKTFMEKECTSIKSAWINSSEFIH 66 Query: 62 ATPAMLDEQCIVVL-CSLNATAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVYS 120 +TP I+ L C T ET+ A +L E GA I +T +E+ G Y + Y+ Sbjct: 67 STPKDFGVNSIICLACHKGNTPETIAAAKLGKEMGATVIILTWLKESEIIEFGDYIIRYT 126 Query: 121 NGEQQIYSQGNQS-------SVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYI 173 + +G+ L ++ E+L Q E Y +Y E + +I+ +AR ++ Sbjct: 127 FDASPDHLKGDIDYSGEKTICALLVAVELLAQTEGYENYNKFYEGTKMISNIIKNARNHV 186 Query: 174 KRIASRFADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFET 233 A +FA++YKDD + Y M SG +Y AY + C FMEMQW ++ +H+GEYFHGPFE Sbjct: 187 AERAVKFAEKYKDDSVIYTMGSGASYGAAYMESICIFMEMQWLNSSSIHTGEYFHGPFEI 246 Query: 234 TDKNIPIILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIM 293 TD N P ++ +SEG+TR LDERAL FL YA+R+ V+DAKELG++ ID SV +YF + Sbjct: 247 TDANRPFMIQISEGSTRDLDERALKFLNTYAKRIEVLDAKELGLSTIDASVVDYFNHSLF 306 Query: 294 IPVERDIVSRMAEIRNHSMNMRRYMWKVEY 323 V +A R H + RRYMWKVEY Sbjct: 307 NNVYPIYNHELATKREHPLPTRRYMWKVEY 336 >ref|NP_391141.1| similar to opine catabolism [Bacillus subtilis] pir||G70018 opine catabolism homolog yurP - Bacillus subtilis emb|CAB15251.1| yurP [Bacillus subtilis subsp. subtilis str. 168] Length = 328 Score = 244 bits (623), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 119/323 (36%), Positives = 186/323 (57%), Gaps = 6/323 (1%) Query: 2 EIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVH 61 E++ + D++ K + H++FVACGGS A ++ Y+ +E+++ + +Y+++EF+ Sbjct: 11 EVQAFLQDLKGKT-----IDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSANEFIQ 65 Query: 62 ATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVYS 120 P L E+ +V+LCS + T ETV+A A +GA TIAMT P + +A Y Y Sbjct: 66 RNPVQLGEKSLVILCSHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQYVAQYD 125 Query: 121 NGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASRF 180 G++ + N + +I F L E ++ A+E ++ ++ A A +F Sbjct: 126 WGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQAIEGLDQLQAVYEKALKQEADNAKQF 185 Query: 181 ADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIPI 240 A ++ + I Y MASG Y AY+ + C MEMQW H+ +H+GEYFHGPFE D+++P Sbjct: 186 AKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIIDESVPF 245 Query: 241 ILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERDI 300 I+ + TRPL+ERALTF +Y +++TV+DA IDDSV Y +++ V R Sbjct: 246 IILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNRVLRSY 305 Query: 301 VSRMAEIRNHSMNMRRYMWKVEY 323 +AE RNH ++ RRYMWKVEY Sbjct: 306 ADELAEERNHPLSHRRYMWKVEY 328 >ref|NP_459564.1| putative phosphosugar isomerases [Salmonella typhimurium LT2] gb|AAL19523.1| putative phosphosugar isomerases [Salmonella typhimurium LT2] Length = 328 Score = 241 bits (616), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 128/324 (39%), Positives = 181/324 (55%), Gaps = 8/324 (2%) Query: 4 KDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVHAT 63 + IISDI K ++ ++FV CGGS + G Y + EA S+EFVHAT Sbjct: 9 RRIISDILGKQN----IERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHAT 64 Query: 64 PAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVYSNG 122 P L + +V+L S TAETV A ++A E+GA TI + P T + Y + Y Sbjct: 65 PKALGKNSVVILASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWA 124 Query: 123 ---EQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 E +Q + L ++ EIL Q E YA Y + AF + +V A+ ++ A R Sbjct: 125 RYPETVDPAQQKAAYSLWLALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQR 184 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA E+KD+++ Y+M SGP++ A+ + C +EMQW ++ +HSGEYFHGPFE T+ P Sbjct: 185 FAAEWKDEKVVYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTP 244 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 IL S G TRPLD+RA+ F+ RY ++ +IDA +LGI + V EYF ++ V Sbjct: 245 FILLQSSGRTRPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDV 304 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +A RNH + RRYMWKVEY Sbjct: 305 YNLALATARNHPLTTRRYMWKVEY 328 >ref|ZP_00036355.1| COG2222: Predicted phosphosugar isomerases [Enterococcus faecium] Length = 321 Score = 231 bits (589), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 120/325 (36%), Positives = 187/325 (57%), Gaps = 6/325 (1%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M K+I+ +I +K KE +K I FV CG S+A ++ Y + + A+ S Y +SEF Sbjct: 1 MNSKEIVKEIVAK-KE---VKSIVFVGCGASKADLYPAKYFLDQNAKQLRISHYTASEFN 56 Query: 61 HATPAMLDEQCIVVLCSLNATA-ETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 +ATPA +DE +V+ SL T ETV+A +A E+GA I++T + + + Y + + Sbjct: 57 YATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSIDSPLTKDADYVIYH 116 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 E L ++ E+LHQ E Y HY+A E F + + + ++ A + Sbjct: 117 RFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIYDLAQKSAESARKDAKK 176 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA+ +KD+++ YVM+SG + AY+ + C MEMQW ++ HSGE+FHGPFE DK++P Sbjct: 177 FAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTFHSGEFFHGPFEIVDKDVP 236 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINI-IDDSVAEYFLSVIMIPVER 298 IL +++G TR +D RALTFL R+ + V+DA + G++ I V +YF ++ V R Sbjct: 237 FILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSSHIAKEVLDYFNPFVITAVFR 296 Query: 299 DIVSRMAEIRNHSMNMRRYMWKVEY 323 ++E R H + RRYMWK+ Y Sbjct: 297 VYAEELSEAREHPLTKRRYMWKLTY 321 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 446,874,645 Number of extensions: 17130537 Number of successful extensions: 40570 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 40622 Number of HSP's successfully gapped: 57 Length of query: 323 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 197 Effective length of database: 415,135,104 Effective search space: 81781615488 Effective search space used: 81781615488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)