BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0529.0008.p (1252 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clo... 133 3e-29 ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellu... 123 2e-26 gb|AAP48997.1| cellulosomal bifunctional enzyme-binding/scaffold... 122 5e-26 gb|AAP48996.1| cellulosomal scaffoldin anchoring protein C; ScaC... 120 2e-25 ref|ZP_00061799.1| hypothetical protein [Clostridium thermocellu... 111 1e-22 >ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clostridium thermocellum ATCC 27405] sp|Q06853|SLP2_CLOTM CELL SURFACE GLYCOPROTEIN 2 PRECURSOR (S-LAYER PROTEIN 2) pir||T18263 S-layer protein - Clostridium thermocellum emb|CAA47842.1| S-layer protein [Clostridium thermocellum] Length = 688 Score = 133 bits (335), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 72/188 (38%), Positives = 103/188 (54%), Gaps = 15/188 (7%) Query: 1076 YMTAPANGLFRPNTAISRYETIAALAPLLDMEE---VNVGNLFNDVTAEHQALTA--FFA 1130 Y+T + +FRP +I+R E A LL E +N + DV + H A A F + Sbjct: 483 YLTGYPDKMFRPEKSITRAEAAVIFAKLLGANENTKINYNVSYTDVDSSHWASWAIKFVS 542 Query: 1131 SAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQS-------DIKGHWSE 1183 + GYPDG F + +TRAEFS T V L V+ G+ ++ D KGHW++ Sbjct: 543 YKKLFTGYPDGSFKPNQNITRAEFS---TVVFKLLVSEKGLKEEKIEKSKFGDTKGHWAQ 599 Query: 1184 KYVNALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ LS GY+ G+PDGTFKP I R+++V LINR G L+ F+D+P +HWA Sbjct: 600 QFIEQLSDLGYINGYPDGTFKPNNNIKRSESVALINRAMGRGPLHGAPQVFEDVPQTHWA 659 Query: 1244 YKDIMSAV 1251 +KDI V Sbjct: 660 FKDIAEGV 667 >ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellum ATCC 27405] sp|Q06848|ANCA_CLOTM Cellulosome anchoring protein precursor pir||T18264 cellulosome anchoring protein - Clostridium thermocellum emb|CAA47843.1| cellulosome anchoring protein [Clostridium thermocellum] Length = 447 Score = 123 bits (309), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 68/179 (37%), Positives = 96/179 (53%), Gaps = 10/179 (5%) Query: 1083 GLFRPNTAISRYETIAALAPLLDMEEVNVGN----LFNDVTAEHQALTA--FFASAGIIE 1136 GLF+P I+R E A LL +E + G F D+ H A A + + Sbjct: 249 GLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIKYVTEQNLFG 308 Query: 1137 GYPDGGFGGTKGLTRAEFS----KIMTAVLNLDVTAAGVTKQSDIKGHWSEKYVNALSKA 1192 GYPDG F K +TRAEF+ K + + ++ T+ DI+GHW++KY+ L Sbjct: 309 GYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDIEGHWAQKYIETLVAK 368 Query: 1193 GYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKDIMSAV 1251 GY++G+PD TF+P A I RA++V LINR LN + F D+P ++WAYKDI V Sbjct: 369 GYIKGYPDETFRPQASIKRAESVALINRSLERGPLNGAVLEFTDVPVNYWAYKDIAEGV 427 Score = 57.8 bits (138), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 38/128 (29%), Positives = 61/128 (47%), Gaps = 9/128 (7%) Query: 1132 AGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAG----VTKQSDIKGHWSEKYVN 1187 A ++GYP G F +TRAE + I +L D +AG +T + HW+ + Sbjct: 240 APFLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIK 299 Query: 1188 ALSKAGYVQGFPDGTFKPGAPITRAQAVVL----INRIAGTKQLNVTTVRFKDLPASHWA 1243 +++ G+PDGTF P ITRA+ + + ++ +Q + KD+ HWA Sbjct: 300 YVTEQNLFGGYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDI-EGHWA 358 Query: 1244 YKDIMSAV 1251 K I + V Sbjct: 359 QKYIETLV 366 >gb|AAP48997.1| cellulosomal bifunctional enzyme-binding/scaffoldin-anchoring protein D; ScaD [Acetivibrio cellulolyticus] Length = 852 Score = 122 bits (307), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 64/181 (35%), Positives = 98/181 (54%), Gaps = 5/181 (2%) Query: 1072 GEVK-YMTAPANGLFRPNTAISRYETIAALAPLLDM-EEVNVGNL-FNDVTAEHQALTA- 1127 GE K Y+ + FRP I+R E +A +D+ N N+ F DVT +H A + Sbjct: 651 GEHKSYLKGYTDNCFRPKKEITRAEAAVIIAKFVDVGSSANSNNVNFPDVTNKHWAKESI 710 Query: 1128 -FFASAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQSDIKGHWSEKYV 1186 G+ +GYPDG F + + R EF+ ++ +L+LD + +DI HW++ ++ Sbjct: 711 ELVTKVGLFKGYPDGSFKPDQTIKRGEFAAVIYNLLDLDASTGLSNNFTDIDDHWAKSFI 770 Query: 1187 NALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKD 1246 L+ Y+ G+ DGTFKP I R + VVL+NR LN T+ F D+P ++WA+KD Sbjct: 771 LKLANLRYINGYSDGTFKPDNKIKRDECVVLVNRALNRGPLNGATLDFTDVPKNYWAFKD 830 Query: 1247 I 1247 I Sbjct: 831 I 831 >gb|AAP48996.1| cellulosomal scaffoldin anchoring protein C; ScaC [Acetivibrio cellulolyticus] Length = 1237 Score = 120 bits (302), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 67/170 (39%), Positives = 96/170 (56%), Gaps = 4/170 (2%) Query: 1082 NGLFRPNTAISRYETIAALAPL--LDMEEVNVGNLFNDVTAEHQALTA--FFASAGIIEG 1137 NGLF P+ I+R E LA L D+ V+ F DV H AL A + G +G Sbjct: 1047 NGLFLPDNNITRAEAATILAKLSGADVTYVSDKVSFPDVKDTHWALWAIKYTTDKGYFKG 1106 Query: 1138 YPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQSDIKGHWSEKYVNALSKAGYVQG 1197 Y DG F + +TRAEF+ I+ L ++ T K D+KGHW++ Y+ L++ Y+ G Sbjct: 1107 YTDGTFKPDQKITRAEFATIVYHFLGIEDTNITKYKFEDVKGHWAQVYIEKLTELKYISG 1166 Query: 1198 FPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKDI 1247 +P+G F+P A I R ++V L+NR L TT +F D+P +HWA+KDI Sbjct: 1167 YPNGNFEPQASIKRCESVALLNRALKRGPLYGTTQKFPDVPTTHWAFKDI 1216 >ref|ZP_00061799.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 631 Score = 111 bits (277), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 60/183 (32%), Positives = 97/183 (53%), Gaps = 12/183 (6%) Query: 1076 YMTAPANGLFRPNTAISRYETIAALAPLL------DMEEVNVGNLFNDVTAEHQALTAFF 1129 Y+ + F+P+ I+R E LA ++ ++EE F DV H A Sbjct: 435 YINGYPDNSFKPDNHITRAEIATILANVMGLSVPDNIEETG----FKDVDKNHWAARYIK 490 Query: 1130 A--SAGIIEGYPDGGFGGTKGLTRAEFSKIMTAVLNLDVTAAGVTKQSDIKGHWSEKYVN 1187 A AGI +GY D F + ++RAE + ++ L+LD A + +D KGHW+ ++ Sbjct: 491 AVTDAGIFKGYEDSTFRPNETISRAELATVIFKCLDLDEKKAVKSSFTDTKGHWASDFIE 550 Query: 1188 ALSKAGYVQGFPDGTFKPGAPITRAQAVVLINRIAGTKQLNVTTVRFKDLPASHWAYKDI 1247 +S+ ++G+ DG+FKP +TRA+AVV+IN + +NV + +F DL HWA+ I Sbjct: 551 EVSRNKIIRGYEDGSFKPNGKVTRAEAVVMINNMLYRDPINVDSSKFTDLDKKHWAFGHI 610 Query: 1248 MSA 1250 +A Sbjct: 611 EAA 613 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,620,823,747 Number of extensions: 65227923 Number of successful extensions: 128993 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 128942 Number of HSP's successfully gapped: 132 Length of query: 1252 Length of database: 757,237,578 Length adjustment: 137 Effective length of query: 1115 Effective length of database: 385,269,015 Effective search space: 429574951725 Effective search space used: 429574951725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)